Restriction map for
pFN21AA0136
(Circular) (Six-base) MAPSORT of: pFN21AA0136.seq Check: 2874 from: 1 to: 7522
pFN21AA0136 7522 bp
With 182 enzymes: SgfI *
February 27, 2009 14:45 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5332 278
Size: 53 83 186 936 3796 2468
Fragments arranged by size:
3796 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4865 1937
Size: 2928 4594
Acc65I G'GTAC_C
Cuts at: 4843 4843
Size: 7522
AclI AA'CG_TT
Cuts at: 1563 5649 6022 1563
Size: 4086 373 3063
Fragments arranged by size:
4086 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2242 3381 3951 4047 4593 4724 5585 6969
Size: 610 1139 570 96 546 131 861 1384
Cuts at: 6969 7237 1632
Size: 268 1917
Fragments arranged by size:
1917 1384 1139 861 610 570 546 268
131 96
AflII C'TTAA_G
Cuts at: 820 1017 3059 820
Size: 197 2042 5283
Fragments arranged by size:
5283 2042 197
AflIII A'CryG_T
Cuts at: 1203 4378 6421 7398 1203
Size: 3175 2043 977 1327
Fragments arranged by size:
3175 2043 1327 977
AgeI A'CCGG_T
Cuts at: 6353 6353
Size: 7522
AhdI GACnn_n'nnGTC
Cuts at: 6251 6251
Size: 7522
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 7522
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 7522
AlwNI CAG_nnn'CTG
Cuts at: 2105 5265 6837 2105
Size: 3160 1572 2790
Fragments arranged by size:
3160 2790 1572
ApaLI G'TGCA_C
Cuts at: 5579 6735 5579
Size: 1156 6366
ApoI r'AATT_y
Cuts at: 1493 2068 2664 2882 3190 3422 3556 3697 4831
Size: 575 596 218 308 232 134 141 1134
Cuts at: 4831 5057 5209 7318 1493
Size: 226 152 2109 1697
Fragments arranged by size:
2109 1697 1134 596 575 308 232 226
218 152 141 134
AseI AT'TA_AT
Cuts at: 160 1032 2111 2475 3459 4281 6076 160
Size: 872 1079 364 984 822 1795 1606
Fragments arranged by size:
1795 1606 1079 984 872 822 364
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7522
AvaI C'yCGr_G
Cuts at: 1870 1943 2669 4847 1870
Size: 73 726 2178 4545
Fragments arranged by size:
4545 2178 726 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 3918 3951 3918
Size: 33 7489
BamHI G'GATC_C
Cuts at: 4852 5219 4852
Size: 367 7155
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 4843 6298 618
Size: 325 217 627 3056 1455 1842
Fragments arranged by size:
3056 1842 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 4841 1391
Size: 3450 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2831 3541 3978 928
Size: 971 932 710 437 4472
Fragments arranged by size:
4472 971 932 710 437
BbvCI CC'TCA_GC
Cuts at: 2036 2036
Size: 7522
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4049 4083 5713 5747 1903
Size: 34 2112 34 1630 34 3678
Fragments arranged by size:
3678 2112 1630 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2022 4188 5414 6624 2022
Size: 2166 1226 1210 2920
Fragments arranged by size:
2920 2166 1226 1210
BclI T'GATC_A
Cuts at: 1548 3223 4168 1548
Size: 1675 945 4902
Fragments arranged by size:
4902 1675 945
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2009 6133 136
Size: 107 122 71 631 605 337 4124 1525
Fragments arranged by size:
4124 1525 631 605 337 122 107 71
BglII A'GATC_T
Cuts at: 7517 7517
Size: 7522
BlpI GC'TnA_GC
Cuts at: 2246 4939 2246
Size: 2693 4829
Bme1580I G_kGCm'C
Cuts at: 1790 5583 6739 1790
Size: 3793 1156 2573
Fragments arranged by size:
3793 2573 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 7522
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2285 5253 6211
Size: 416 53 83 399 24 868 2968 958
Cuts at: 6211 442
Size: 1753
Fragments arranged by size:
2968 1753 958 868 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7522
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4688 4688
Size: 7522
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 6182 1239
Size: 4943 2579
Bpu10I CC'TnA_GC
Cuts at: 2036 2036
Size: 7522
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 5647 6512 6810 5647
Size: 865 298 6359
Fragments arranged by size:
6359 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 6185 882
Size: 617 4686 2219
Fragments arranged by size:
4686 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2810 5313 493
Size: 711 1606 2503 2702
Fragments arranged by size:
2702 2503 1606 711
BsaBI GATnn'nnATC
Cuts at: 2871 4734 7516 2871
Size: 1863 2782 2877
Fragments arranged by size:
2877 2782 1863
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 5329 5711 6346 275
Size: 53 83 186 936 3796 382 635 1451
Fragments arranged by size:
3796 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 3439 5955 6353 6627 6774 7185 3439
Size: 2516 398 274 147 411 3776
Fragments arranged by size:
3776 2516 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3667 3697 1178
Size: 30 165 30 2264 30 5003
Fragments arranged by size:
5003 2264 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 4655 1186
Size: 3469 4053
BseYI C'CCAG_C
Cuts at: 6725 7221 6725
Size: 496 7026
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2118 4466 1163
Size: 573 382 2348 4219
Fragments arranged by size:
4219 2348 573 382
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4972 5733 6761 1540
Size: 275 186 2971 761 1028 2301
Fragments arranged by size:
2971 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 5583 5668 6739 5583
Size: 85 1071 6366
Fragments arranged by size:
6366 1071 85
BsmI GAATG_Cn'
Cuts at: 2450 2799 3667 4397 5033 5126 2450
Size: 349 868 730 636 93 4846
Fragments arranged by size:
4846 868 730 636 349 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 4841 5583 5668 6739 1391
Size: 399 3051 742 85 1071 2174
Fragments arranged by size:
3051 2174 1071 742 399 85
BspEI T'CCGG_A
Cuts at: 7185 7185
Size: 7522
BspHI T'CATG_A
Cuts at: 3778 5411 7141 3778
Size: 1633 1730 4159
Fragments arranged by size:
4159 1730 1633
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3775 4877 844
Size: 2931 1102 3489
Fragments arranged by size:
3489 2931 1102
BsrBI CCG'CTC
Cuts at: 1394 1823 5409 1394
Size: 429 3586 3507
Fragments arranged by size:
3586 3507 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 6017 6191 65
Size: 1183 4769 174 1396
Fragments arranged by size:
4769 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 6166 6353 1700
Size: 110 4356 187 2869
Fragments arranged by size:
4356 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 7522
BssHII G'CGCG_C
Cuts at: 1925 2040 1925
Size: 115 7407
BssSI C'ACGA_G
Cuts at: 5582 6594 5582
Size: 1012 6510
BstAPI GCAn_nnn'nTGC
Cuts at: 794 4076 794
Size: 3282 4240
BstBI TT'CG_AA
Cuts at: 2840 6326 2840
Size: 3486 4036
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 7522
BstYI r'GATC_y
Cuts at: 1972 4852 5219 5604 5621 7073 7159 7171 7517
Size: 2880 367 385 17 1452 86 12 346
Cuts at: 7517 1972
Size: 1977
Fragments arranged by size:
2880 1977 1452 385 367 346 86 17
12
Bsu36I CC'TnA_GG
Cuts at: 2756 3516 2756
Size: 760 6762
BtgI C'CryG_G
Cuts at: 513 1065 2004 2023 2532 513
Size: 552 939 19 509 5503
Fragments arranged by size:
5503 939 552 509 19
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6783
Fragments arranged by size:
6783 683 56
BtsI GCAGTG_nn'
Cuts at: 4005 5038 5832 5852 4005
Size: 1033 794 20 5675
Fragments arranged by size:
5675 1033 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7522
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 7487
DraI TTT'AAA
Cuts at: 4826 4826
Size: 7522
DraIII CAC_nnn'GTG
Cuts at: 4556 4556
Size: 7522
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 6529 809
Size: 507 5213 1802
Fragments arranged by size:
5213 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2087 3479 3862
Size: 54 1425 50 275 18 257 1392 383
Cuts at: 3862 4969 5858 7487 8
Size: 1107 889 1629 43
Fragments arranged by size:
1629 1425 1392 1107 889 383 275 257
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4969 1537
Size: 275 3157 4090
Fragments arranged by size:
4090 3157 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2837 4692 5452 1353
Size: 1484 1855 760 3423
Fragments arranged by size:
3423 1855 1484 760
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3277 6109 6483 6629 387
Size: 1064 138 1688 2832 374 146 1280
Fragments arranged by size:
2832 1688 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2242 3381 3951 4047 4593 4724 5585
Size: 393 610 1139 570 96 546 131 861
Cuts at: 5585 6182 6969 7237 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 1139 861 787 610 597 570 546
393 268 131 96
EcoRI G'AATT_C
Cuts at: 2664 4831 5209 2664
Size: 2167 378 4977
Fragments arranged by size:
4977 2167 378
EcoRV GAT'ATC
Cuts at: 2659 2659
Size: 7522
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2516 3525 2516
Size: 1009 6513
FspI TGC'GCA
Cuts at: 6028 6028
Size: 7522
HaeII r_GCGC'y
Cuts at: 1678 6669 1678
Size: 4991 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3779 3811 6163 6195 6237
Size: 32 135 32 2207 32 2352 32 42
Cuts at: 6237 6269 1373
Size: 32 2626
Fragments arranged by size:
2626 2352 2207 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 4696 4866 5112 669
Size: 988 281 2758 170 246 3079
Fragments arranged by size:
3079 2758 988 281 246 170
HindIII A'AGCT_T
Cuts at: 748 2248 2979 3502 4329 748
Size: 1500 731 523 827 3941
Fragments arranged by size:
3941 1500 827 731 523
HpaI GTT'AAC
Cuts at: 4696 5112 4696
Size: 416 7106
KpnI G_GTAC'C
Cuts at: 4847 4847
Size: 7522
MfeI C'AATT_G
Cuts at: 5121 5121
Size: 7522
MluI A'CGCG_T
Cuts at: 7398 7398
Size: 7522
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2275 2974 3007 3016 6636 6820 1305
Size: 970 699 33 9 3620 184 2007
Fragments arranged by size:
3620 2007 970 699 184 33 9
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2089 3864 10
Size: 54 1425 343 257 1775 3668
Fragments arranged by size:
3668 1775 1425 343 257 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2381 3895 4659 5480 5839 5998 518
Size: 648 1215 1514 764 821 359 159 2042
Fragments arranged by size:
2042 1514 1215 821 764 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2025 2833 4938 5615 6763 7008 1606
Size: 419 808 2105 677 1148 245 2120
Fragments arranged by size:
2120 2105 1148 808 677 419 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 6031
Fragments arranged by size:
6031 939 552
NdeI CA'TA_TG
Cuts at: 387 4158 4308 4656 387
Size: 3771 150 348 3253
Fragments arranged by size:
3771 3253 348 150
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7522
NotI GC'GGCC_GC
Cuts at: 4969 4969
Size: 7522
NspI r_CATG'y
Cuts at: 1132 1229 2432 4382 4880 6425 1132
Size: 97 1203 1950 498 1545 2229
Fragments arranged by size:
2229 1950 1545 1203 498 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 7522
PciI A'CATG_T
Cuts at: 4378 6421 4378
Size: 2043 5479
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 7522
PmeI GTTT'AAAC
Cuts at: 4826 4826
Size: 7522
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5591 5623 7104 7136 5591
Size: 32 1481 32 5977
Fragments arranged by size:
5977 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7522
PsiI TTA'TAA
Cuts at: 3708 4143 5092 3708
Size: 435 949 6138
Fragments arranged by size:
6138 949 435
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 2529 2561 2529
Size: 32 7490
PstI C_TGCA'G
Cuts at: 830 2927 3772 3919 4072 4874 830
Size: 2097 845 147 153 802 3478
Fragments arranged by size:
3478 2097 845 802 153 147
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7522
PvuII CAG'CTG
Cuts at: 2833 2833
Size: 7522
SacII CC_GC'GG
Cuts at: 2026 2026
Size: 7522
SalI G'TCGA_C
Cuts at: 1936 4864 1936
Size: 2928 4594
SbfI CC_TGCA'GG
Cuts at: 4874 4874
Size: 7522
ScaI AGT'ACT
Cuts at: 4819 5770 7482 4819
Size: 951 1712 4859
Fragments arranged by size:
4859 1712 951
SfcI C'TryA_G
Cuts at: 826 1045 2923 3768 3915 4068 4870 6005 6686
Size: 219 1878 845 147 153 802 1135 681
Cuts at: 6686 6877 826
Size: 191 1471
Fragments arranged by size:
1878 1471 1135 845 802 681 219 191
153 147
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7522
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7522
SmaI CCC'GGG
Cuts at: 1872 4849 1872
Size: 2977 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3059 5626 6527 6789 820
Size: 197 926 1116 2567 901 262 1553
Fragments arranged by size:
2567 1553 1116 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 5313 493
Size: 4820 2702
SpeI A'CTAG_T
Cuts at: 152 2075 152
Size: 1923 5599
SphI G_CATG'C
Cuts at: 1132 4880 1132
Size: 3748 3774
SspI AAT'ATT
Cuts at: 5 52 5446 5
Size: 47 5394 2081
Fragments arranged by size:
5394 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 7522
StyI C'CwwG_G
Cuts at: 513 1065 2004 4961 513
Size: 552 939 2957 3074
Fragments arranged by size:
3074 2957 939 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3011 3833 5549 5566 5719 5904 6349
Size: 1255 1431 822 1716 17 153 185 445
Cuts at: 6349 325
Size: 1498
Fragments arranged by size:
1716 1498 1431 1255 822 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3102 4817 5768
Size: 274 80 33 51 1443 1125 1715 951
Cuts at: 5768 7480 96
Size: 1712 138
Fragments arranged by size:
1715 1712 1443 1125 951 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2805 2837 7482 7514 2805
Size: 32 4645 32 2813
Fragments arranged by size:
4645 2813 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2267 4152 1316
Size: 951 1885 4686
Fragments arranged by size:
4686 1885 951
XbaI T'CTAG_A
Cuts at: 4858 4858
Size: 7522
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7522
XmaI C'CCGG_G
Cuts at: 1870 4847 1870
Size: 2977 4545
XmnI GAAnn'nnTTC
Cuts at: 2312 3525 3556 5651 2312
Size: 1213 31 2095 4183
Fragments arranged by size:
4183 2095 1213 31
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5330 276
Size: 53 83 186 936 3796 2468
Fragments arranged by size:
3796 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BaeI BamHI BanI BanII BbsI BbvCI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I
BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI
EaeI EagI EarI EciI Eco57MI EcoRI EcoRV FalI
FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI
MluI MmeI MscI MslI MspA1I NcoI NdeI NheI
NotI NspI PasI PciI PflMI PmeI PpiI PshAI
PsiI PsrI PstI PvuI PvuII SacII SalI SbfI
ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BbeI BfrBI
BsiWI BsmBI BstXI BstZ17I EcoICRI EcoNI EcoO109I FseI
FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI
PfoI PmlI PpuMI PspOMI PspXI RsrII SacI SanDI
SapI SexAI SfiI SfoI SrfI SwaI XcmI