Restriction map for
pFN21AA0133
(Circular) (Six-base) MAPSORT of: pFN21AA0133.seq Check: 8283 from: 1 to: 9277
pFN21AA0133 9277 bp
With 182 enzymes: SgfI *
October 20, 2009 10:37 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2410 2613 2674 3030 2410
Size: 203 61 356 8657
Fragments arranged by size:
8657 356 203 61
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 6462 7087 278
Size: 53 83 186 936 4926 625 2468
Fragments arranged by size:
4926 2468 936 625 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2396 6620 1937
Size: 459 4224 4594
Fragments arranged by size:
4594 4224 459
Acc65I G'GTAC_C
Cuts at: 6598 6598
Size: 9277
AclI AA'CG_TT
Cuts at: 1563 7404 7777 1563
Size: 5841 373 3063
Fragments arranged by size:
5841 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2210 2273 3260 3285 4104 6028 6098 7340
Size: 578 63 987 25 819 1924 70 1242
Cuts at: 7340 8724 8992 1632
Size: 1384 268 1917
Fragments arranged by size:
1924 1917 1384 1242 987 819 578 268
70 63 25
AflII C'TTAA_G
Cuts at: 820 1017 2033 820
Size: 197 1016 8064
Fragments arranged by size:
8064 1016 197
AflIII A'CryG_T
Cuts at: 1203 3540 5778 6363 8176 9153 1203
Size: 2337 2238 585 1813 977 1327
Fragments arranged by size:
2337 2238 1813 1327 977 585
AgeI A'CCGG_T
Cuts at: 8108 8108
Size: 9277
AhdI GACnn_n'nnGTC
Cuts at: 3544 5768 8006 3544
Size: 2224 2238 4815
Fragments arranged by size:
4815 2238 2224
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 9277
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2267 3615 3704 1184
Size: 1083 1348 89 6757
Fragments arranged by size:
6757 1348 1083 89
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3845 3877 4613 4645 3845
Size: 32 736 32 8477
Fragments arranged by size:
8477 736 32 32
AlwNI CAG_nnn'CTG
Cuts at: 2437 2689 3449 3535 3751 5230 5294 5488 5642
Size: 252 760 86 216 1479 64 194 154
Cuts at: 5642 5764 6469 7020 8592 2437
Size: 122 705 551 1572 3122
Fragments arranged by size:
3122 1572 1479 760 705 551 252 216
194 154 122 86 64
ApaI G_GGCC'C
Cuts at: 3485 3847 3903 5326 3485
Size: 362 56 1423 7436
Fragments arranged by size:
7436 1423 362 56
ApaLI G'TGCA_C
Cuts at: 6276 7334 8490 6276
Size: 1058 1156 7063
Fragments arranged by size:
7063 1156 1058
ApoI r'AATT_y
Cuts at: 1493 3141 4991 5646 6586 6812 6964 9073 1493
Size: 1648 1850 655 940 226 152 2109 1697
Fragments arranged by size:
2109 1850 1697 1648 940 655 226 152
AseI AT'TA_AT
Cuts at: 160 1032 2283 7831 160
Size: 872 1251 5548 1606
Fragments arranged by size:
5548 1606 1251 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 9277
AvaI C'yCGr_G
Cuts at: 1870 1943 3478 5325 6106 6602 1870
Size: 73 1535 1847 781 496 4545
Fragments arranged by size:
4545 1847 1535 781 496 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 4019 4052 4019
Size: 33 9244
BamHI G'GATC_C
Cuts at: 2345 6607 6974 2345
Size: 4262 367 4648
Fragments arranged by size:
4648 4262 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 4481 5334 6598 8053 618
Size: 325 217 627 2694 853 1264 1455 1842
Fragments arranged by size:
2694 1842 1455 1264 853 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2778 3485 3741 3847 3903 5326 6516 6596
Size: 1387 707 256 106 56 1423 1190 80
Cuts at: 6596 1391
Size: 4072
Fragments arranged by size:
4072 1423 1387 1190 707 256 106 80
56
BbeI G_GCGC'C
Cuts at: 5338 5338
Size: 9277
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2810 3248 3364 6094 928
Size: 971 911 438 116 2730 4111
Fragments arranged by size:
4111 2730 971 911 438 116
BbvCI CC'TCA_GC
Cuts at: 2678 3450 5825 6321 2678
Size: 772 2375 496 5634
Fragments arranged by size:
5634 2375 772 496
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 7468 7502 1903
Size: 34 5531 34 3678
Fragments arranged by size:
5531 3678 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4277 7169 8379 4277
Size: 2892 1210 5175
Fragments arranged by size:
5175 2892 1210
BclI T'GATC_A
Cuts at: 1548 5518 1548
Size: 3970 5307
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3353 3608 7888
Size: 107 122 71 631 605 1681 255 4280
Cuts at: 7888 136
Size: 1525
Fragments arranged by size:
4280 1681 1525 631 605 255 122 107
71
BglII A'GATC_T
Cuts at: 3924 9272 3924
Size: 5348 3929
BlpI GC'TnA_GC
Cuts at: 2453 2690 3186 5054 6694 2453
Size: 237 496 1868 1640 5036
Fragments arranged by size:
5036 1868 1640 496 237
Bme1580I G_kGCm'C
Cuts at: 1790 2651 3485 3847 3903 4486 5326 6280 7338
Size: 861 834 362 56 583 840 954 1058
Cuts at: 7338 8494 1790
Size: 1156 2573
Fragments arranged by size:
2573 1156 1058 954 861 840 834 583
362 56
BmgBI CAC'GTC
Cuts at: 1318 2579 6031 1318
Size: 1261 3452 4564
Fragments arranged by size:
4564 3452 1261
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2500 6352 7008
Size: 416 53 83 399 24 1083 3852 656
Cuts at: 7008 7966 442
Size: 958 1753
Fragments arranged by size:
3852 1753 1083 958 656 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 9277
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2594 3533 3578 3941 4447 4682 5741 6107
Size: 1355 939 45 363 506 235 1059 366
Cuts at: 6107 7937 1239
Size: 1830 2579
Fragments arranged by size:
2579 1830 1355 1059 939 506 366 363
235 45
Bpu10I CC'TnA_GC
Cuts at: 2678 2748 3450 5825 6321 2678
Size: 70 702 2375 496 5634
Fragments arranged by size:
5634 2375 702 496 70
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2249 4805 5752 6515 7402 8267 8565 2249
Size: 2556 947 763 887 865 298 2961
Fragments arranged by size:
2961 2556 947 887 865 763 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 7940 882
Size: 617 6441 2219
Fragments arranged by size:
6441 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3181 3543 6364 7068 493
Size: 711 1977 362 2821 704 2702
Fragments arranged by size:
2821 2702 1977 711 704 362
BsaBI GATnn'nnATC
Cuts at: 5039 9271 5039
Size: 4232 5045
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 5335 6459 7084 7466
Size: 53 83 186 936 3802 1124 625 382
Cuts at: 7466 8101 275
Size: 635 1451
Fragments arranged by size:
3802 1451 1124 936 635 625 382 186
83 53
BsaWI w'CCGG_w
Cuts at: 4077 7710 8108 8382 8529 8940 4077
Size: 3633 398 274 147 411 4414
Fragments arranged by size:
4414 3633 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2484 2514 1178
Size: 30 165 30 1081 30 7941
Fragments arranged by size:
7941 1081 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2252 2745 3044 3679 4620 4795 4798 5036
Size: 1066 493 299 635 941 175 3 238
Cuts at: 5036 6020 6338 1186
Size: 984 318 4125
Fragments arranged by size:
4125 1066 984 941 635 493 318 299
238 175 3
BseYI C'CCAG_C
Cuts at: 4485 5230 5245 8480 8976 4485
Size: 745 15 3235 496 4786
Fragments arranged by size:
4786 3235 745 496 15
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2898 3626 3764 5816 5970 6098 1163
Size: 573 1162 728 138 2052 154 128 4342
Fragments arranged by size:
4342 2052 1162 728 573 154 138 128
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 6576 6727 7488 8516 1540
Size: 275 186 4575 151 761 1028 2301
Fragments arranged by size:
4575 2301 1028 761 275 186 151
BsiHKAI G_wGCw'C
Cuts at: 3741 3825 4879 5067 6126 6280 6516 7338 7423
Size: 84 1054 188 1059 154 236 822 85
Cuts at: 7423 8494 3741
Size: 1071 4524
Fragments arranged by size:
4524 1071 1059 1054 822 236 188 154
85 84
BsmI GAATG_Cn'
Cuts at: 4467 5087 6788 6881 4467
Size: 620 1701 93 6863
Fragments arranged by size:
6863 1701 620 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2651 2778 3485 3741 3825 3847 3903
Size: 399 861 127 707 256 84 22 56
Cuts at: 3903 4486 4879 5067 5326 6126 6280 6516 6596
Size: 583 393 188 259 800 154 236 80
Cuts at: 6596 7338 7423 8494 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1071 861 800 742 707 583 399
393 259 256 236 188 154 127 85
84 80 56 22
BspEI T'CCGG_A
Cuts at: 8940 8940
Size: 9277
BspHI T'CATG_A
Cuts at: 7166 8896 7166
Size: 1730 7547
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2410 2554 2613 2674 3030 3300 4047 5263
Size: 1566 144 59 61 356 270 747 1216
Cuts at: 5263 6264 6632 844
Size: 1001 368 3489
Fragments arranged by size:
3489 1566 1216 1001 747 368 356 270
144 61 59
BsrBI CCG'CTC
Cuts at: 1394 1823 7164 1394
Size: 429 5341 3507
Fragments arranged by size:
5341 3507 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 7772 7946 65
Size: 1183 6524 174 1396
Fragments arranged by size:
6524 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3220 7921 8108 1700
Size: 110 1410 4701 187 2869
Fragments arranged by size:
4701 2869 1410 187 110
BsrGI T'GTAC_A
Cuts at: 96 6297 96
Size: 6201 3076
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 9277
BssSI C'ACGA_G
Cuts at: 3740 6315 7337 8349 3740
Size: 2575 1022 1012 4668
Fragments arranged by size:
4668 2575 1022 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3469 3751 5278 6121 794
Size: 2675 282 1527 843 3950
Fragments arranged by size:
3950 2675 1527 843 282
BstBI TT'CG_AA
Cuts at: 3334 8081 3334
Size: 4747 4530
BstEII G'GTnAC_C
Cuts at: 1184 6382 1184
Size: 5198 4079
BstXI CCAn_nnnn'nTGG
Cuts at: 2914 3795 4850 2914
Size: 881 1055 7341
Fragments arranged by size:
7341 1055 881
BstYI r'GATC_y
Cuts at: 1972 2217 2345 3531 3924 5638 6607 6974 7359
Size: 245 128 1186 393 1714 969 367 385
Cuts at: 7359 7376 8828 8914 8926 9272 1972
Size: 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1714 1452 1186 969 393 385 367
346 245 128 86 17 12
Bsu36I CC'TnA_GG
Cuts at: 2627 6150 2627
Size: 3523 5754
BtgI C'CryG_G
Cuts at: 513 1065 2004 2835 3404 3489 4317 4773 513
Size: 552 939 831 569 85 828 456 5017
Fragments arranged by size:
5017 939 831 828 569 552 456 85
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 3112 4125 6292 487
Size: 683 56 1886 1013 2167 3472
Fragments arranged by size:
3472 2167 1886 1013 683 56
BtsI GCAGTG_nn'
Cuts at: 3643 5395 5753 5819 5987 6146 6793 7587 7607
Size: 1752 358 66 168 159 647 794 20
Cuts at: 7607 3643
Size: 5313
Fragments arranged by size:
5313 1752 794 647 358 168 159 66
20
ClaI AT'CG_AT
Cuts at: 1554 3312 1554
Size: 1758 7519
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 9242
DraI TTT'AAA
Cuts at: 6581 6581
Size: 9277
DraIII CAC_nnn'GTG
Cuts at: 5852 6121 5852
Size: 269 9008
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 8284 809
Size: 507 6968 1802
Fragments arranged by size:
6968 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2424 2536 2665
Size: 54 1425 50 275 18 594 112 129
Cuts at: 2665 2904 3105 4177 4290 4776 6573 6724 7613
Size: 239 201 1072 113 486 1797 151 889
Cuts at: 7613 9242 8
Size: 1629 43
Fragments arranged by size:
1797 1629 1425 1072 889 594 486 275
239 201 151 129 113 112 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 6573 6724 1537
Size: 275 4761 151 4090
Fragments arranged by size:
4761 4090 275 151
EarI CTCTTCn'nnn_
Cuts at: 1353 3147 3411 4344 5620 6131 7207 1353
Size: 1794 264 933 1276 511 1076 3423
Fragments arranged by size:
3423 1794 1276 1076 933 511 264
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 7864 8238 8384 387
Size: 1064 138 6275 374 146 1280
Fragments arranged by size:
6275 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 3739 6514 3739
Size: 2775 6502
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2210 2273 2594 3260 3285 3533 3578
Size: 393 578 63 321 666 25 248 45
Cuts at: 3578 3941 4104 4447 4682 5741 6028 6098 6107
Size: 363 163 343 235 1059 287 70 9
Cuts at: 6107 7340 7937 8724 8992 1239
Size: 1233 597 787 268 1524
Fragments arranged by size:
1524 1233 1059 787 666 597 578 393
363 343 321 287 268 248 235 163
70 63 45 25 9
EcoNI CCTnn'n_nnAGG
Cuts at: 3026 5465 3026
Size: 2439 6838
EcoO109I rG'GnC_Cy
Cuts at: 3482 3900 5207 5242 5322 3482
Size: 418 1307 35 80 7437
Fragments arranged by size:
7437 1307 418 80 35
EcoRI G'AATT_C
Cuts at: 3141 4991 6586 6964 3141
Size: 1850 1595 378 5454
Fragments arranged by size:
5454 1850 1595 378
EcoRV GAT'ATC
Cuts at: 3252 3252
Size: 9277
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2153 4406 4663 5074 6403 2153
Size: 2253 257 411 1329 5027
Fragments arranged by size:
5027 2253 1329 411 257
FspI TGC'GCA
Cuts at: 7783 7783
Size: 9277
HaeII r_GCGC'y
Cuts at: 1678 3359 5338 8424 1678
Size: 1681 1979 3086 2531
Fragments arranged by size:
3086 2531 1979 1681
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2269 2301 2741 2773 3424
Size: 32 135 32 697 32 440 32 651
Cuts at: 3424 3456 3530 3562 4403 4435 4960 4992 5199
Size: 32 74 32 841 32 525 32 207
Cuts at: 5199 5231 5754 5786 6376 6408 7918 7950 7992
Size: 32 523 32 590 32 1510 32 42
Cuts at: 7992 8024 1373
Size: 32 2626
Fragments arranged by size:
2626 1510 841 697 651 590 525 523
440 207 135 74 42 32 32 32
32 32 32 32 32 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2397 2569 6621 6867 669
Size: 988 281 459 172 4052 246 3079
Fragments arranged by size:
4052 3079 988 459 281 246 172
HindIII A'AGCT_T
Cuts at: 748 2036 2180 4144 5925 748
Size: 1288 144 1964 1781 4100
Fragments arranged by size:
4100 1964 1781 1288 144
HpaI GTT'AAC
Cuts at: 6867 6867
Size: 9277
KasI G'GCGC_C
Cuts at: 5334 5334
Size: 9277
KpnI G_GTAC'C
Cuts at: 6602 6602
Size: 9277
MfeI C'AATT_G
Cuts at: 6876 6876
Size: 9277
MluI A'CGCG_T
Cuts at: 9153 9153
Size: 9277
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3418 4656 5472 6348 8391 8575 1305
Size: 2113 1238 816 876 2043 184 2007
Fragments arranged by size:
2113 2043 2007 1238 876 816 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2538 2667 2906 3107 4179
Size: 54 1425 343 706 129 239 201 1072
Cuts at: 4179 4292 10
Size: 113 4995
Fragments arranged by size:
4995 1425 1072 706 343 239 201 129
113 54
MslI CAynn'nnrTG
Cuts at: 518 1166 4547 5045 5702 7235 7594 7753 518
Size: 648 3381 498 657 1533 359 159 2042
Fragments arranged by size:
3381 2042 1533 657 648 498 359 159
MspA1I CmG'CkG
Cuts at: 1606 2677 3234 3446 4033 4660 4720 5813 6085
Size: 1071 557 212 587 627 60 1093 272
Cuts at: 6085 6398 6693 7370 8518 8763 1606
Size: 313 295 677 1148 245 2120
Fragments arranged by size:
2120 1148 1093 1071 677 627 587 557
313 295 272 245 212 60
NarI GG'CG_CC
Cuts at: 5335 5335
Size: 9277
NcoI C'CATG_G
Cuts at: 513 1065 2004 2835 4317 4773 513
Size: 552 939 831 1482 456 5017
Fragments arranged by size:
5017 1482 939 831 552 456
NdeI CA'TA_TG
Cuts at: 387 387
Size: 9277
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 9277
NotI GC'GGCC_GC
Cuts at: 6724 6724
Size: 9277
NspI r_CATG'y
Cuts at: 1132 1229 3507 5782 6635 8180 1132
Size: 97 2278 2275 853 1545 2229
Fragments arranged by size:
2278 2275 2229 1545 853 97
PasI CC'CwG_GG
Cuts at: 1348 5155 1348
Size: 3807 5470
PciI A'CATG_T
Cuts at: 5778 8176 5778
Size: 2398 6879
PflMI CCAn_nnn'nTGG
Cuts at: 1108 5203 5764 5852 1108
Size: 4095 561 88 4533
Fragments arranged by size:
4533 4095 561 88
PfoI T'CCnGG_A
Cuts at: 3510 3534 3765 6435 3510
Size: 24 231 2670 6352
Fragments arranged by size:
6352 2670 231 24
PmeI GTTT'AAAC
Cuts at: 6581 6581
Size: 9277
PmlI CAC'GTG
Cuts at: 3181 3543 3181
Size: 362 8915
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2708 2740 3146 3178 7346 7378 8859 8891 2708
Size: 32 406 32 4168 32 1481 32 3094
Fragments arranged by size:
4168 3094 1481 406 32 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 5207 5207
Size: 9277
PshAI GACnn'nnGTC
Cuts at: 1613 4497 5388 1613
Size: 2884 891 5502
Fragments arranged by size:
5502 2884 891
PsiI TTA'TAA
Cuts at: 2172 6847 2172
Size: 4675 4602
PspOMI G'GGCC_C
Cuts at: 3481 3843 3899 5322 3481
Size: 362 56 1423 7436
Fragments arranged by size:
7436 1423 362 56
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 2118 2150 2118
Size: 32 9245
PstI C_TGCA'G
Cuts at: 830 2480 2559 3027 3804 4044 5271 5286 5304
Size: 1650 79 468 777 240 1227 15 18
Cuts at: 5304 5402 6141 6417 6486 6629 830
Size: 98 739 276 69 143 3478
Fragments arranged by size:
3478 1650 1227 777 739 468 276 240
143 98 79 69 18 15
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 9277
PvuII CAG'CTG
Cuts at: 2677 3446 4033 4660 4720 5813 6398 2677
Size: 769 587 627 60 1093 585 5556
Fragments arranged by size:
5556 1093 769 627 587 585 60
SacI G_AGCT'C
Cuts at: 3741 6516 3741
Size: 2775 6502
SalI G'TCGA_C
Cuts at: 1936 2395 6619 1936
Size: 459 4224 4594
Fragments arranged by size:
4594 4224 459
SapI GCTCTTCn'nnn_
Cuts at: 6131 6131
Size: 9277
SbfI CC_TGCA'GG
Cuts at: 2480 3027 6629 2480
Size: 547 3602 5128
Fragments arranged by size:
5128 3602 547
ScaI AGT'ACT
Cuts at: 7525 9237 7525
Size: 1712 7565
SexAI A'CCwGG_T
Cuts at: 5186 5186
Size: 9277
SfcI C'TryA_G
Cuts at: 826 1045 2476 2555 3023 3800 3874 4040 4212
Size: 219 1431 79 468 777 74 166 172
Cuts at: 4212 5267 5282 5300 5398 6137 6413 6482 6625
Size: 1055 15 18 98 739 276 69 143
Cuts at: 6625 7760 8441 8632 826
Size: 1135 681 191 1471
Fragments arranged by size:
1471 1431 1135 1055 777 739 681 468
276 219 191 172 166 143 98 79
74 69 18 15
SfoI GGC'GCC
Cuts at: 5336 5336
Size: 9277
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 9277
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 9277
SmaI CCC'GGG
Cuts at: 1872 5327 6604 1872
Size: 3455 1277 4545
Fragments arranged by size:
4545 3455 1277
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2033 2264 4820 5731 6530 7381
Size: 197 926 90 231 2556 911 799 851
Cuts at: 7381 8282 8544 820
Size: 901 262 1553
Fragments arranged by size:
2556 1553 926 911 901 851 799 262
231 197 90
SnaBI TAC'GTA
Cuts at: 493 7068 493
Size: 6575 2702
SpeI A'CTAG_T
Cuts at: 152 4070 152
Size: 3918 5359
SphI G_CATG'C
Cuts at: 1132 3507 6635 1132
Size: 2375 3128 3774
Fragments arranged by size:
3774 3128 2375
SspI AAT'ATT
Cuts at: 5 52 7201 5
Size: 47 7149 2081
Fragments arranged by size:
7149 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 9277
StyI C'CwwG_G
Cuts at: 513 1065 2004 2533 2835 4317 4773 5425 6716
Size: 552 939 529 302 1482 456 652 1291
Cuts at: 6716 513
Size: 3074
Fragments arranged by size:
3074 1482 1291 939 652 552 529 456
302
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3229 5335 5714 7304 7321 7474 7659
Size: 1255 1649 2106 379 1590 17 153 185
Cuts at: 7659 8104 325
Size: 445 1498
Fragments arranged by size:
2106 1649 1590 1498 1255 445 379 185
153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4924 6297 7523
Size: 274 80 33 51 1443 2947 1373 1226
Cuts at: 7523 9235 96
Size: 1712 138
Fragments arranged by size:
2947 1712 1443 1373 1226 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4180 4212 9237 9269 4180
Size: 32 5025 32 4188
Fragments arranged by size:
5025 4188 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 9277
XbaI T'CTAG_A
Cuts at: 2938 6613 2938
Size: 3675 5602
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3073 3073
Size: 9277
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 9277
XmaI C'CCGG_G
Cuts at: 1870 5325 6602 1870
Size: 3455 1277 4545
Fragments arranged by size:
4545 3455 1277
XmnI GAAnn'nnTTC
Cuts at: 6188 7406 6188
Size: 1218 8059
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 6460 7085 276
Size: 53 83 186 936 4926 625 2468
Fragments arranged by size:
4926 2468 936 625 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII
BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII
BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI
CspCI DraI DraIII DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI
HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI
MluI MmeI MscI MslI MspA1I NarI NcoI NdeI
NheI NotI NspI PasI PciI PflMI PfoI PmeI
PmlI PpiI PpuMI PshAI PsiI PspOMI PsrI PstI
PvuI PvuII SacI SalI SapI SbfI ScaI SexAI
SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AscI AvrII BfrBI BplI BsiWI BsmBI BstZ17I
FseI FspAI NaeI NgoMIV NruI NsiI PacI PspXI
RsrII SacII SanDI SfiI SrfI SwaI