Restriction map for
pFN21AA0130
(Circular) (Six-base) MAPSORT of: pFN21AA0130.seq Check: 4661 from: 1 to: 7672
pFN21AA0130 7672 bp
With 182 enzymes: SgfI *
January 16, 2009 15:22 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5482 278
Size: 53 83 186 936 3946 2468
Fragments arranged by size:
3946 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4176 4227 5015 1937
Size: 2239 51 788 4594
Fragments arranged by size:
4594 2239 788 51
Acc65I G'GTAC_C
Cuts at: 4993 4993
Size: 7672
AclI AA'CG_TT
Cuts at: 1563 5799 6172 1563
Size: 4236 373 3063
Fragments arranged by size:
4236 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2042 2216 2474 3050 4283 5735 7119 7387
Size: 410 174 258 576 1233 1452 1384 268
Cuts at: 7387 1632
Size: 1917
Fragments arranged by size:
1917 1452 1384 1233 576 410 268 258
174
AfeI AGC'GCT
Cuts at: 2054 3065 2054
Size: 1011 6661
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 7475
AflIII A'CryG_T
Cuts at: 1203 2310 6571 7548 1203
Size: 1107 4261 977 1327
Fragments arranged by size:
4261 1327 1107 977
AgeI A'CCGG_T
Cuts at: 6503 6503
Size: 7672
AhdI GACnn_n'nnGTC
Cuts at: 6401 6401
Size: 7672
AleI CACnn'nnGTG
Cuts at: 1166 3662 4076 4856 1166
Size: 2496 414 780 3982
Fragments arranged by size:
3982 2496 780 414
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2368 4850 1184
Size: 1184 2482 4006
Fragments arranged by size:
4006 2482 1184
AlwNI CAG_nnn'CTG
Cuts at: 2417 2462 2497 2791 3563 5415 6987 2417
Size: 45 35 294 772 1852 1572 3102
Fragments arranged by size:
3102 1852 1572 772 294 45 35
ApaI G_GGCC'C
Cuts at: 2533 3475 2533
Size: 942 6730
ApaLI G'TGCA_C
Cuts at: 5729 6885 5729
Size: 1156 6516
ApoI r'AATT_y
Cuts at: 1493 2090 3404 3422 4981 5207 5359 7468 1493
Size: 597 1314 18 1559 226 152 2109 1697
Fragments arranged by size:
2109 1697 1559 1314 597 226 152 18
AseI AT'TA_AT
Cuts at: 160 1032 6226 160
Size: 872 5194 1606
Fragments arranged by size:
5194 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7672
AvaI C'yCGr_G
Cuts at: 1870 1943 2271 2753 2894 4417 4997 1870
Size: 73 328 482 141 1523 580 4545
Fragments arranged by size:
4545 1523 580 482 328 141 73
BamHI G'GATC_C
Cuts at: 5002 5369 5002
Size: 367 7305
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2660 2759 3568 4993 6448
Size: 325 217 627 873 99 809 1425 1455
Cuts at: 6448 618
Size: 1842
Fragments arranged by size:
1842 1455 1425 873 809 627 325 217
99
BanII G_rGCy'C
Cuts at: 1391 2374 2533 2883 2913 3249 3475 4592 4625
Size: 983 159 350 30 336 226 1117 33
Cuts at: 4625 4991 1391
Size: 366 4072
Fragments arranged by size:
4072 1117 983 366 350 336 226 159
33 30
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 4524 928
Size: 971 2625 4076
Fragments arranged by size:
4076 2625 971
BbvCI CC'TCA_GC
Cuts at: 2510 3564 4922 2510
Size: 1054 1358 5260
Fragments arranged by size:
5260 1358 1054
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4558 4592 5863 5897 1903
Size: 34 2621 34 1271 34 3678
Fragments arranged by size:
3678 2621 1271 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 5564 6774 5564
Size: 1210 6462
BclI T'GATC_A
Cuts at: 1548 3996 1548
Size: 2448 5224
BfrBI ATG'CAT
Cuts at: 2355 2355
Size: 7672
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4439 6283 136
Size: 107 122 71 631 605 2767 1844 1525
Fragments arranged by size:
2767 1844 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2856 7667 2856
Size: 4811 2861
BlpI GC'TnA_GC
Cuts at: 3153 4586 5089 3153
Size: 1433 503 5736
Fragments arranged by size:
5736 1433 503
Bme1580I G_kGCm'C
Cuts at: 1790 2533 2762 3475 3573 4424 4456 5733 6889
Size: 743 229 713 98 851 32 1277 1156
Cuts at: 6889 1790
Size: 2573
Fragments arranged by size:
2573 1277 1156 851 743 713 229 98
32
BmgBI CAC'GTC
Cuts at: 1318 3886 4033 1318
Size: 2568 147 4957
Fragments arranged by size:
4957 2568 147
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4762 5403 6361
Size: 416 53 83 399 24 3345 641 958
Cuts at: 6361 442
Size: 1753
Fragments arranged by size:
3345 1753 958 641 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7672
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2578 2578
Size: 7672
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2077 2439 2462 2519 2873 4034 4181 4829
Size: 838 362 23 57 354 1161 147 648
Cuts at: 4829 6332 1239
Size: 1503 2579
Fragments arranged by size:
2579 1503 1161 838 648 362 354 147
57 23
Bpu10I CC'TnA_GC
Cuts at: 2510 3564 4922 2510
Size: 1054 1358 5260
Fragments arranged by size:
5260 1358 1054
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2897 2921 3251 5797 6662 6960 2897
Size: 24 330 2546 865 298 3609
Fragments arranged by size:
3609 2546 865 330 298 24
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3575 4749 6335 882
Size: 617 2076 1174 1586 2219
Fragments arranged by size:
2219 2076 1586 1174 617
BsaAI yAC'GTr
Cuts at: 493 1204 5463 493
Size: 711 4259 2702
Fragments arranged by size:
4259 2702 711
BsaBI GATnn'nnATC
Cuts at: 7666 7666
Size: 7672
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4224 4487 5479 5861
Size: 53 83 186 936 2691 263 992 382
Cuts at: 5861 6496 275
Size: 635 1451
Fragments arranged by size:
2691 1451 992 936 635 382 263 186
83 53
BsaWI w'CCGG_w
Cuts at: 2637 3298 3888 6105 6503 6777 6924 7335 2637
Size: 661 590 2217 398 274 147 411 2974
Fragments arranged by size:
2974 2217 661 590 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2900 2930 3552 3582 4007
Size: 30 165 30 1497 30 622 30 425
Cuts at: 4007 4037 4169 4199 1178
Size: 30 132 30 4651
Fragments arranged by size:
4651 1497 622 425 165 132 30 30
30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2570 2921 3623 3685 4378 4459 4720 4805
Size: 1384 351 702 62 693 81 261 85
Cuts at: 4805 1186
Size: 4053
Fragments arranged by size:
4053 1384 702 693 351 261 85 81
62
BseYI C'CCAG_C
Cuts at: 2462 3183 3310 3895 4170 4354 6875 7371 2462
Size: 721 127 585 275 184 2521 496 2763
Fragments arranged by size:
2763 2521 721 585 496 275 184 127
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2389 2522 3734 4196 4580 4796 4950
Size: 573 653 133 1212 462 384 216 154
Cuts at: 4950 1163
Size: 3885
Fragments arranged by size:
3885 1212 653 573 462 384 216 154
133
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 5122 5883 6911 1540
Size: 275 186 3121 761 1028 2301
Fragments arranged by size:
3121 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2913 3177 4284 4592 5733 5818 6889 2913
Size: 264 1107 308 1141 85 1071 3696
Fragments arranged by size:
3696 1141 1107 1071 308 264 85
BsmI GAATG_Cn'
Cuts at: 2357 2584 3208 5183 5276 2357
Size: 227 624 1975 93 4753
Fragments arranged by size:
4753 1975 624 227 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2774 4262 2774
Size: 1488 6184
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2374 2533 2762 2883 2913 3177 3249
Size: 399 584 159 229 121 30 264 72
Cuts at: 3249 3475 3573 4284 4424 4456 4592 4625 4991
Size: 226 98 711 140 32 136 33 366
Cuts at: 4991 5733 5818 6889 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 711 584 399 366 264
229 226 159 140 136 121 98 85
72 33 32 30
BspEI T'CCGG_A
Cuts at: 2637 3888 7335 2637
Size: 1251 3447 2974
Fragments arranged by size:
3447 2974 1251
BspHI T'CATG_A
Cuts at: 3999 5561 7291 3999
Size: 1562 1730 4380
Fragments arranged by size:
4380 1730 1562
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3605 4110 5027 844
Size: 2761 505 917 3489
Fragments arranged by size:
3489 2761 917 505
BsrBI CCG'CTC
Cuts at: 1394 1823 2425 4185 5559 1394
Size: 429 602 1760 1374 3507
Fragments arranged by size:
3507 1760 1374 602 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2291 4959 6167 6341 65
Size: 1183 1043 2668 1208 174 1396
Fragments arranged by size:
2668 1396 1208 1183 1043 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2447 2720 3457 6316 6503 1700
Size: 110 637 273 737 2859 187 2869
Fragments arranged by size:
2869 2859 737 637 273 187 110
BsrGI T'GTAC_A
Cuts at: 96 3080 96
Size: 2984 4688
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7672
BssSI C'ACGA_G
Cuts at: 5732 6744 5732
Size: 1012 6660
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2417 4288 4843 794
Size: 1623 1871 555 3623
Fragments arranged by size:
3623 1871 1623 555
BstBI TT'CG_AA
Cuts at: 4568 6476 4568
Size: 1908 5764
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 7672
BstXI CCAn_nnnn'nTGG
Cuts at: 2011 3104 3524 3664 4078 4159 2011
Size: 1093 420 140 414 81 5524
Fragments arranged by size:
5524 1093 420 414 140 81
BstYI r'GATC_y
Cuts at: 1972 2607 2856 3618 5002 5369 5754 5771 7223
Size: 635 249 762 1384 367 385 17 1452
Cuts at: 7223 7309 7321 7667 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 1384 762 635 385 367 346
249 86 17 12
Bsu36I CC'TnA_GG
Cuts at: 3606 3606
Size: 7672
BtgI C'CryG_G
Cuts at: 513 1065 4822 513
Size: 552 3757 3363
Fragments arranged by size:
3757 3363 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2527 487
Size: 683 56 1301 5632
Fragments arranged by size:
5632 1301 683 56
BtsI GCAGTG_nn'
Cuts at: 2660 4302 4422 4784 5188 5982 6002 2660
Size: 1642 120 362 404 794 20 4330
Fragments arranged by size:
4330 1642 794 404 362 120 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7672
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2934 2969 529
Size: 35 2370 35 5232
Fragments arranged by size:
5232 2370 35 35
DraI TTT'AAA
Cuts at: 4976 4976
Size: 7672
DraIII CAC_nnn'GTG
Cuts at: 2743 2743
Size: 7672
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2282 6679 809
Size: 507 966 4397 1802
Fragments arranged by size:
4397 1802 966 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 4440 4707 4819
Size: 54 1425 50 275 18 2610 267 112
Cuts at: 4819 4908 5119 6008 7637 8
Size: 89 211 889 1629 43
Fragments arranged by size:
2610 1629 1425 889 275 267 211 112
89 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 5119 1537
Size: 275 3307 4090
Fragments arranged by size:
4090 3307 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2236 2569 3335 4499 4547 5602 1353
Size: 883 333 766 1164 48 1055 3423
Fragments arranged by size:
3423 1164 1055 883 766 333 48
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3257 3635 3923 6259 6633 6779
Size: 1064 138 1668 378 288 2336 374 146
Cuts at: 6779 387
Size: 1280
Fragments arranged by size:
2336 1668 1280 1064 378 374 288 146
138
EcoICRI GAG'CTC
Cuts at: 2911 4590 2911
Size: 1679 5993
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2042 2077 2216 2439 2462 2474 2519
Size: 393 410 35 139 223 23 12 45
Cuts at: 2519 2873 3050 4034 4181 4283 4829 5735 6332
Size: 354 177 984 147 102 546 906 597
Cuts at: 6332 7119 7387 1239
Size: 787 268 1524
Fragments arranged by size:
1524 984 906 787 597 546 410 393
354 268 223 177 147 139 102 45
35 23 12
EcoNI CCTnn'n_nnAGG
Cuts at: 2753 4250 4811 2753
Size: 1497 561 5614
Fragments arranged by size:
5614 1497 561
EcoO109I rG'GnC_Cy
Cuts at: 2275 2530 3472 3790 4687 2275
Size: 255 942 318 897 5260
Fragments arranged by size:
5260 942 897 318 255
EcoRI G'AATT_C
Cuts at: 3422 4981 5359 3422
Size: 1559 378 5735
Fragments arranged by size:
5735 1559 378
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2110 2740 2110
Size: 630 7042
FspI TGC'GCA
Cuts at: 6178 6178
Size: 7672
HaeII r_GCGC'y
Cuts at: 1678 2056 2385 2497 3067 6819 1678
Size: 378 329 112 570 3752 2531
Fragments arranged by size:
3752 2531 570 378 329 112
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2771 2803 4169 4201 6313
Size: 32 135 32 1199 32 1366 32 2112
Cuts at: 6313 6345 6387 6419 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 2112 1366 1199 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2017 3007 3139 4678 5016 5262
Size: 988 281 79 990 132 1539 338 246
Cuts at: 5262 669
Size: 3079
Fragments arranged by size:
3079 1539 990 988 338 281 246 132
79
HindIII A'AGCT_T
Cuts at: 748 2865 748
Size: 2117 5555
HpaI GTT'AAC
Cuts at: 5262 5262
Size: 7672
KpnI G_GTAC'C
Cuts at: 4997 4997
Size: 7672
MfeI C'AATT_G
Cuts at: 5271 5271
Size: 7672
MluI A'CGCG_T
Cuts at: 7548 7548
Size: 7672
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3027 4842 6786 6970 1305
Size: 1722 1815 1944 184 2007
Fragments arranged by size:
2007 1944 1815 1722 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 4709 4910 10
Size: 54 1425 343 2877 201 2772
Fragments arranged by size:
2877 2772 1425 343 201 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2009 2309 3662 4076 4856 5630 5989
Size: 648 843 300 1353 414 780 774 359
Cuts at: 5989 6148 518
Size: 159 2042
Fragments arranged by size:
2042 1353 843 780 774 648 414 359
300 159
MspA1I CmG'CkG
Cuts at: 1606 2462 2803 3229 3940 4754 5088 5765 6913
Size: 856 341 426 711 814 334 677 1148
Cuts at: 6913 7158 1606
Size: 245 2120
Fragments arranged by size:
2120 1148 856 814 711 677 426 341
334 245
NaeI GCC'GGC
Cuts at: 2449 2449
Size: 7672
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 7120
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7672
NgoMIV G'CCGG_C
Cuts at: 2447 2447
Size: 7672
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7672
NotI GC'GGCC_GC
Cuts at: 5119 5119
Size: 7672
NsiI A_TGCA'T
Cuts at: 2357 2357
Size: 7672
NspI r_CATG'y
Cuts at: 1132 1229 2314 2702 2824 3331 3604 4024 4333
Size: 97 1085 388 122 507 273 420 309
Cuts at: 4333 4684 5030 6575 1132
Size: 351 346 1545 2229
Fragments arranged by size:
2229 1545 1085 507 420 388 351 346
309 273 122 97
PasI CC'CwG_GG
Cuts at: 1348 4933 1348
Size: 3585 4087
PciI A'CATG_T
Cuts at: 2310 6571 2310
Size: 4261 3411
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2462 1108
Size: 1354 6318
PfoI T'CCnGG_A
Cuts at: 2850 3335 4011 2850
Size: 485 676 6511
Fragments arranged by size:
6511 676 485
PmeI GTTT'AAAC
Cuts at: 4976 4976
Size: 7672
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5741 5773 7254 7286 5741
Size: 32 1481 32 6127
Fragments arranged by size:
6127 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2275 3790 4687 2275
Size: 1515 897 5260
Fragments arranged by size:
5260 1515 897
PshAI GACnn'nnGTC
Cuts at: 1613 4346 4486 1613
Size: 2733 140 4799
Fragments arranged by size:
4799 2733 140
PsiI TTA'TAA
Cuts at: 5242 5242
Size: 7672
PspOMI G'GGCC_C
Cuts at: 2529 3471 2529
Size: 942 6730
PspXI vC'TCGA_Gb
Cuts at: 2753 2753
Size: 7672
PstI C_TGCA'G
Cuts at: 830 2422 3927 3975 4041 4839 5024 830
Size: 1592 1505 48 66 798 185 3478
Fragments arranged by size:
3478 1592 1505 798 185 66 48
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7672
PvuII CAG'CTG
Cuts at: 2803 3229 3940 2803
Size: 426 711 6535
Fragments arranged by size:
6535 711 426
RsrII CG'GwC_CG
Cuts at: 3166 3166
Size: 7672
SacI G_AGCT'C
Cuts at: 2913 4592 2913
Size: 1679 5993
SalI G'TCGA_C
Cuts at: 1936 5014 1936
Size: 3078 4594
SanDI GG'GwC_CC
Cuts at: 4687 4687
Size: 7672
SbfI CC_TGCA'GG
Cuts at: 5024 5024
Size: 7672
ScaI AGT'ACT
Cuts at: 2972 5920 7632 2972
Size: 2948 1712 3012
Fragments arranged by size:
3012 2948 1712
SfcI C'TryA_G
Cuts at: 826 1045 2418 3923 3971 4037 4835 5020 6155
Size: 219 1373 1505 48 66 798 185 1135
Cuts at: 6155 6836 7027 826
Size: 681 191 1471
Fragments arranged by size:
1505 1471 1373 1135 798 681 219 191
185 66 48
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7672
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7672
SmaI CCC'GGG
Cuts at: 1872 4419 4999 1872
Size: 2547 580 4545
Fragments arranged by size:
4545 2547 580
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2753 2912 2936 3266 5776 6677
Size: 197 926 810 159 24 330 2510 901
Cuts at: 6677 6939 820
Size: 262 1553
Fragments arranged by size:
2510 1553 926 901 810 330 262 197
159 24
SnaBI TAC'GTA
Cuts at: 493 5463 493
Size: 4970 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7672
SphI G_CATG'C
Cuts at: 1132 2702 2824 3604 5030 1132
Size: 1570 122 780 1426 3774
Fragments arranged by size:
3774 1570 1426 780 122
SspI AAT'ATT
Cuts at: 5 52 5596 5
Size: 47 5544 2081
Fragments arranged by size:
5544 2081 47
StuI AGG'CCT
Cuts at: 1517 2561 3734 1517
Size: 1044 1173 5455
Fragments arranged by size:
5455 1173 1044
StyI C'CwwG_G
Cuts at: 513 1065 4152 4896 5111 513
Size: 552 3087 744 215 3074
Fragments arranged by size:
3087 3074 744 552 215
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3183 4647 4781 5699 5716 5869 6054
Size: 1255 1603 1464 134 918 17 153 185
Cuts at: 6054 6499 325
Size: 445 1498
Fragments arranged by size:
1603 1498 1464 1255 918 445 185 153
134 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2970 3080 5918
Size: 274 80 33 51 1443 993 110 2838
Cuts at: 5918 7630 96
Size: 1712 138
Fragments arranged by size:
2838 1712 1443 993 274 138 110 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2832 2864 2900 2932 3317 3349 3552 3584 4007
Size: 32 36 32 385 32 203 32 423
Cuts at: 4007 4039 7632 7664 2832
Size: 32 3593 32 2840
Fragments arranged by size:
3593 2840 423 385 203 36 32 32
32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2785 1316
Size: 1469 6203
XbaI T'CTAG_A
Cuts at: 5008 5008
Size: 7672
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 4321 4367 4897 4903 4321
Size: 46 530 6 7090
Fragments arranged by size:
7090 530 46 6
XhoI C'TCGA_G
Cuts at: 1943 2753 1943
Size: 810 6862
XmaI C'CCGG_G
Cuts at: 1870 4417 4997 1870
Size: 2547 580 4545
Fragments arranged by size:
4545 2547 580
XmnI GAAnn'nnTTC
Cuts at: 2090 3392 3422 5801 2090
Size: 1302 30 2379 3961
Fragments arranged by size:
3961 2379 1302 30
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5480 276
Size: 53 83 186 936 3946 2468
Fragments arranged by size:
3946 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI
BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI
CspCI DraI DraIII DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI
NotI NsiI NspI PasI PciI PflMI PfoI PmeI
PpiI PpuMI PshAI PsiI PspOMI PspXI PstI PvuI
PvuII RsrII SacI SalI SanDI SbfI ScaI SfcI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AloI AscI AvrII BaeI BbeI BsiWI BstZ17I
EcoRV FseI FspAI KasI NarI NruI PacI PmlI
PsrI SacII SapI SexAI SfiI SfoI SrfI SwaI