Restriction map for
pFN21AA0129
(Circular) (Six-base) MAPSORT of: pFN21AA0129.seq Check: 7145 from: 1 to: 6031
pFN21AA0129 6031 bp
With 182 enzymes: SgfI *
April 15, 2010 09:27 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3252 3252
Size: 6031
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3841 278
Size: 53 83 186 936 2305 2468
Fragments arranged by size:
2468 2305 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3374 1937
Size: 1437 4594
Acc65I G'GTAC_C
Cuts at: 2747 3352 2747
Size: 605 5426
AclI AA'CG_TT
Cuts at: 1563 4158 4531 1563
Size: 2595 373 3063
Fragments arranged by size:
3063 2595 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2357 2522 4094 5478 5746 1632
Size: 725 165 1572 1384 268 1917
Fragments arranged by size:
1917 1572 1384 725 268 165
AfeI AGC'GCT
Cuts at: 2385 3101 2385
Size: 716 5315
AflII C'TTAA_G
Cuts at: 820 1017 2696 820
Size: 197 1679 4155
Fragments arranged by size:
4155 1679 197
AflIII A'CryG_T
Cuts at: 1203 4930 5907 1203
Size: 3727 977 1327
Fragments arranged by size:
3727 1327 977
AgeI A'CCGG_T
Cuts at: 4862 4862
Size: 6031
AhdI GACnn_n'nnGTC
Cuts at: 4760 4760
Size: 6031
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6031
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3164 1184
Size: 1980 4051
AlwNI CAG_nnn'CTG
Cuts at: 2499 3774 5346 2499
Size: 1275 1572 3184
Fragments arranged by size:
3184 1572 1275
ApaI G_GGCC'C
Cuts at: 2901 2901
Size: 6031
ApaLI G'TGCA_C
Cuts at: 2201 4088 5244 2201
Size: 1887 1156 2988
Fragments arranged by size:
2988 1887 1156
ApoI r'AATT_y
Cuts at: 1493 2345 2396 3340 3566 3718 5827 1493
Size: 852 51 944 226 152 2109 1697
Fragments arranged by size:
2109 1697 944 852 226 152 51
AseI AT'TA_AT
Cuts at: 160 1032 4585 160
Size: 872 3553 1606
Fragments arranged by size:
3553 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6031
AvaI C'yCGr_G
Cuts at: 1870 1943 2585 3356 1870
Size: 73 642 771 4545
Fragments arranged by size:
4545 771 642 73
BamHI G'GATC_C
Cuts at: 2818 3361 3728 2818
Size: 543 367 5121
Fragments arranged by size:
5121 543 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2747 2999 3039 3090 3352
Size: 325 217 627 960 252 40 51 262
Cuts at: 3352 4807 618
Size: 1455 1842
Fragments arranged by size:
1842 1455 960 627 325 262 252 217
51 40
BanII G_rGCy'C
Cuts at: 1391 2106 2520 2625 2901 3350 1391
Size: 715 414 105 276 449 4072
Fragments arranged by size:
4072 715 449 414 276 105
BbeI G_GCGC'C
Cuts at: 3003 3043 3003
Size: 40 5991
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3175 928
Size: 971 1276 3784
Fragments arranged by size:
3784 1276 971
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4222 4256 1903
Size: 34 2285 34 3678
Fragments arranged by size:
3678 2285 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3923 5133 3923
Size: 1210 4821
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6031
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2886 4642 136
Size: 107 122 71 631 605 1214 1756 1525
Fragments arranged by size:
1756 1525 1214 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6026 6026
Size: 6031
BlpI GC'TnA_GC
Cuts at: 2100 3448 2100
Size: 1348 4683
Bme1580I G_kGCm'C
Cuts at: 1790 2205 2610 2901 2907 4092 5248 1790
Size: 415 405 291 6 1185 1156 2573
Fragments arranged by size:
2573 1185 1156 415 405 291 6
BmgBI CAC'GTC
Cuts at: 1318 2986 3223 1318
Size: 1668 237 4126
Fragments arranged by size:
4126 1668 237
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2983 3127 3762
Size: 416 53 83 399 24 1566 144 635
Cuts at: 3762 4720 442
Size: 958 1753
Fragments arranged by size:
1753 1566 958 635 416 399 144 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6031
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2210 2411 2521 4691 1239
Size: 971 201 110 2170 2579
Fragments arranged by size:
2579 2170 971 201 110
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2288 3080 3131 4156 5021 5319 2288
Size: 792 51 1025 865 298 3000
Fragments arranged by size:
3000 1025 865 792 298 51
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4694 882
Size: 617 3195 2219
Fragments arranged by size:
3195 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2206 3822 493
Size: 711 1002 1616 2702
Fragments arranged by size:
2702 1616 1002 711
BsaBI GATnn'nnATC
Cuts at: 6025 6025
Size: 6031
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2678 3000 3040 3180
Size: 53 83 186 936 1145 322 40 140
Cuts at: 3180 3204 3838 4220 4855 275
Size: 24 634 382 635 1451
Fragments arranged by size:
1451 1145 936 635 634 382 322 186
140 83 53 40 24
BsaWI w'CCGG_w
Cuts at: 4464 4862 5136 5283 5694 4464
Size: 398 274 147 411 4801
Fragments arranged by size:
4801 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5806
Fragments arranged by size:
5806 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 6031
BseYI C'CCAG_C
Cuts at: 2152 2884 5234 5730 2152
Size: 732 2350 496 2453
Fragments arranged by size:
2453 2350 732 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2228 3008 1163
Size: 573 492 780 4186
Fragments arranged by size:
4186 780 573 492
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2022 2092 2860 2965 3057 3172
Size: 275 186 21 70 768 105 92 115
Cuts at: 3172 3481 4242 5270 1540
Size: 309 761 1028 2301
Fragments arranged by size:
2301 1028 768 761 309 275 186 115
105 92 70 21
BsiHKAI G_wGCw'C
Cuts at: 2106 2205 4092 4177 5248 2106
Size: 99 1887 85 1071 2889
Fragments arranged by size:
2889 1887 1071 99 85
BsmI GAATG_Cn'
Cuts at: 3542 3635 3542
Size: 93 5938
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2106 2205 2520 2610 2625 2901 2907
Size: 399 316 99 315 90 15 276 6
Cuts at: 2907 3350 4092 4177 5248 1391
Size: 443 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 443 399 316 315 276
99 90 85 15 6
BspEI T'CCGG_A
Cuts at: 5694 5694
Size: 6031
BspHI T'CATG_A
Cuts at: 2475 3920 5650 2475
Size: 1445 1730 2856
Fragments arranged by size:
2856 1730 1445
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3252 3386 844
Size: 2408 134 3489
Fragments arranged by size:
3489 2408 134
BsrBI CCG'CTC
Cuts at: 1394 1823 3277 3918 1394
Size: 429 1454 641 3507
Fragments arranged by size:
3507 1454 641 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4526 4700 65
Size: 1183 3278 174 1396
Fragments arranged by size:
3278 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2960 3002 3171 3200 4675 4862 1700
Size: 110 1150 42 169 29 1475 187 2869
Fragments arranged by size:
2869 1475 1150 187 169 110 42 29
BsrGI T'GTAC_A
Cuts at: 96 2433 96
Size: 2337 3694
BssHII G'CGCG_C
Cuts at: 1925 2136 2157 2801 2837 1925
Size: 211 21 644 36 5119
Fragments arranged by size:
5119 644 211 36 21
BssSI C'ACGA_G
Cuts at: 2648 2943 4091 5103 2648
Size: 295 1148 1012 3576
Fragments arranged by size:
3576 1148 1012 295
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2566 2712 794
Size: 1772 146 4113
Fragments arranged by size:
4113 1772 146
BstBI TT'CG_AA
Cuts at: 4835 4835
Size: 6031
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6031
BstYI r'GATC_y
Cuts at: 1972 2818 3361 3728 4113 4130 5582 5668 5680
Size: 846 543 367 385 17 1452 86 12
Cuts at: 5680 6026 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 846 543 385 367 346 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2164 2658 3043 513
Size: 552 939 160 494 385 3501
Fragments arranged by size:
3501 939 552 494 385 160
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5292
Fragments arranged by size:
5292 683 56
BtsI GCAGTG_nn'
Cuts at: 3547 4341 4361 3547
Size: 794 20 5217
Fragments arranged by size:
5217 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6031
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5996
DraI TTT'AAA
Cuts at: 2232 3335 2232
Size: 1103 4928
DraIII CAC_nnn'GTG
Cuts at: 2176 2176
Size: 6031
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5038 809
Size: 507 3722 1802
Fragments arranged by size:
3722 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2167 2388 2550
Size: 54 1425 50 275 18 337 221 162
Cuts at: 2550 2962 3054 3133 3478 4367 5996 8
Size: 412 92 79 345 889 1629 43
Fragments arranged by size:
1629 1425 889 412 345 337 275 221
162 92 79 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2962 3054 3478 1537
Size: 275 1150 92 424 4090
Fragments arranged by size:
4090 1150 424 275 92
EarI CTCTTCn'nnn_
Cuts at: 1353 2111 2370 3961 1353
Size: 758 259 1591 3423
Fragments arranged by size:
3423 1591 758 259
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2842 4618 4992 5138 387
Size: 1064 138 1253 1776 374 146 1280
Fragments arranged by size:
1776 1280 1253 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2104 2104
Size: 6031
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2210 2357 2411 2521 2522 4094 4691
Size: 393 578 147 54 110 1 1572 597
Cuts at: 4691 5478 5746 1239
Size: 787 268 1524
Fragments arranged by size:
1572 1524 787 597 578 393 268 147
110 54 1
EcoO109I rG'GnC_Cy
Cuts at: 2644 2897 3048 2644
Size: 253 151 5627
Fragments arranged by size:
5627 253 151
EcoRI G'AATT_C
Cuts at: 3340 3718 3340
Size: 378 5653
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2653 2653
Size: 6031
FspI TGC'GCA
Cuts at: 4537 4537
Size: 6031
HaeII r_GCGC'y
Cuts at: 1678 2074 2189 2387 3003 3043 3103 5178 1678
Size: 396 115 198 616 40 60 2075 2531
Fragments arranged by size:
2531 2075 616 396 198 115 60 40
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4672 4704 4746 4778 1373
Size: 32 135 32 3100 32 42 32 2626
Fragments arranged by size:
3100 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2983 3375 3621 669
Size: 988 281 1045 392 246 3079
Fragments arranged by size:
3079 1045 988 392 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6031
HpaI GTT'AAC
Cuts at: 3621 3621
Size: 6031
KasI G'GCGC_C
Cuts at: 2999 3039 2999
Size: 40 5991
KpnI G_GTAC'C
Cuts at: 2751 3356 2751
Size: 605 5426
MfeI C'AATT_G
Cuts at: 3630 3630
Size: 6031
MluI A'CGCG_T
Cuts at: 5907 5907
Size: 6031
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2025 2514 5145 5329 1305
Size: 720 489 2631 184 2007
Fragments arranged by size:
2631 2007 720 489 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2169 2390 10
Size: 54 1425 343 337 221 3651
Fragments arranged by size:
3651 1425 343 337 221 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3989 4348 4507 518
Size: 648 2823 359 159 2042
Fragments arranged by size:
2823 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2118 2127 2198 2239 3045 3447 4124 5272
Size: 512 9 71 41 806 402 677 1148
Cuts at: 5272 5517 1606
Size: 245 2120
Fragments arranged by size:
2120 1148 806 677 512 402 245 71
41 9
NaeI GCC'GGC
Cuts at: 3004 3202 3004
Size: 198 5833
NarI GG'CG_CC
Cuts at: 3000 3040 3000
Size: 40 5991
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4540
Fragments arranged by size:
4540 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6031
NgoMIV G'CCGG_C
Cuts at: 3002 3200 3002
Size: 198 5833
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6031
NotI GC'GGCC_GC
Cuts at: 3478 3478
Size: 6031
NspI r_CATG'y
Cuts at: 1132 1229 3389 4934 1132
Size: 97 2160 1545 2229
Fragments arranged by size:
2229 2160 1545 97
PasI CC'CwG_GG
Cuts at: 1348 2036 1348
Size: 688 5343
PciI A'CATG_T
Cuts at: 4930 4930
Size: 6031
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2176 1108
Size: 1068 4963
PfoI T'CCnGG_A
Cuts at: 3186 3267 3186
Size: 81 5950
PmeI GTTT'AAAC
Cuts at: 3335 3335
Size: 6031
PmlI CAC'GTG
Cuts at: 2206 2206
Size: 6031
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4100 4132 5613 5645 4100
Size: 32 1481 32 4486
Fragments arranged by size:
4486 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6031
PsiI TTA'TAA
Cuts at: 3601 3601
Size: 6031
PspOMI G'GGCC_C
Cuts at: 2897 2897
Size: 6031
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 2346 2378 2346
Size: 32 5999
PstI C_TGCA'G
Cuts at: 830 3383 830
Size: 2553 3478
PvuI CG_AT'CG
Cuts at: 2001 2860 2001
Size: 859 5172
PvuII CAG'CTG
Cuts at: 2239 2239
Size: 6031
SacI G_AGCT'C
Cuts at: 2106 2106
Size: 6031
SacII CC_GC'GG
Cuts at: 3046 3046
Size: 6031
SalI G'TCGA_C
Cuts at: 1936 3373 1936
Size: 1437 4594
SapI GCTCTTCn'nnn_
Cuts at: 2111 2111
Size: 6031
SbfI CC_TGCA'GG
Cuts at: 3383 3383
Size: 6031
ScaI AGT'ACT
Cuts at: 3116 4279 5991 3116
Size: 1163 1712 3156
Fragments arranged by size:
3156 1712 1163
SfcI C'TryA_G
Cuts at: 826 1045 3379 4514 5195 5386 826
Size: 219 2334 1135 681 191 1471
Fragments arranged by size:
2334 1471 1135 681 219 191
SfoI GGC'GCC
Cuts at: 3001 3041 3001
Size: 40 5991
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6031
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6031
SmaI CCC'GGG
Cuts at: 1872 3358 1872
Size: 1486 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2303 2696 3095 3110 4135 5036
Size: 197 926 360 393 399 15 1025 901
Cuts at: 5036 5298 820
Size: 262 1553
Fragments arranged by size:
1553 1025 926 901 399 393 360 262
197 15
SnaBI TAC'GTA
Cuts at: 493 3822 493
Size: 3329 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6031
SphI G_CATG'C
Cuts at: 1132 3389 1132
Size: 2257 3774
SspI AAT'ATT
Cuts at: 5 52 3955 5
Size: 47 3903 2081
Fragments arranged by size:
3903 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6031
StyI C'CwwG_G
Cuts at: 513 1065 2004 3470 513
Size: 552 939 1466 3074
Fragments arranged by size:
3074 1466 939 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3001 4058 4075 4228 4413 4858 325
Size: 1255 1421 1057 17 153 185 445 1498
Fragments arranged by size:
1498 1421 1255 1057 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2433 2666 3114
Size: 274 80 33 51 1443 456 233 448
Cuts at: 3114 4277 5989 96
Size: 1163 1712 138
Fragments arranged by size:
1712 1443 1163 456 448 274 233 138
80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2803 2835 5991 6023 2803
Size: 32 3156 32 2811
Fragments arranged by size:
3156 2811 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6031
XbaI T'CTAG_A
Cuts at: 3367 3367
Size: 6031
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6031
XmaI C'CCGG_G
Cuts at: 1870 3356 1870
Size: 1486 4545
XmnI GAAnn'nnTTC
Cuts at: 4160 4160
Size: 6031
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3839 276
Size: 53 83 186 936 2305 2468
Fragments arranged by size:
2468 2305 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI
MfeI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NdeI NgoMIV NheI NotI NspI PasI PciI
PflMI PfoI PmeI PmlI PpiI PshAI PsiI PspOMI
PsrI PstI PvuI PvuII SacI SacII SalI SapI
SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AloI AscI AvrII BaeI BbvCI BfrBI BplI Bpu10I
BsiWI BsmBI BstXI BstZ17I Bsu36I EcoNI EcoRV FseI
FspAI NruI NsiI PacI PpuMI PspXI RsrII SanDI
SexAI SfiI SrfI SwaI XcmI