Restriction map for
pFN21AA0128
(Circular) (Six-base) MAPSORT of: pFN21AA0128.seq Check: 6582 from: 1 to: 5986
pFN21AA0128 5986 bp
With 182 enzymes: SgfI *
January 16, 2009 15:22 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2709 2709
Size: 5986
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3796 278
Size: 53 83 186 936 2260 2468
Fragments arranged by size:
2468 2260 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3329 1937
Size: 1392 4594
Acc65I G'GTAC_C
Cuts at: 3307 3307
Size: 5986
AclI AA'CG_TT
Cuts at: 1563 4113 4486 1563
Size: 2550 373 3063
Fragments arranged by size:
3063 2550 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2510 2756 2941 3137 3281 4049 5433 5701
Size: 878 246 185 196 144 768 1384 268
Cuts at: 5701 1632
Size: 1917
Fragments arranged by size:
1917 1384 878 768 268 246 196 185
144
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5789
AflIII A'CryG_T
Cuts at: 1203 2068 4885 5862 1203
Size: 865 2817 977 1327
Fragments arranged by size:
2817 1327 977 865
AgeI A'CCGG_T
Cuts at: 4817 4817
Size: 5986
AhdI GACnn_n'nnGTC
Cuts at: 4715 4715
Size: 5986
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5986
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5986
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2174 2206 2174
Size: 32 5954
AlwNI CAG_nnn'CTG
Cuts at: 3259 3729 5301 3259
Size: 470 1572 3944
Fragments arranged by size:
3944 1572 470
ApaLI G'TGCA_C
Cuts at: 4043 5199 4043
Size: 1156 4830
ApoI r'AATT_y
Cuts at: 1493 2201 2363 3295 3521 3673 5782 1493
Size: 708 162 932 226 152 2109 1697
Fragments arranged by size:
2109 1697 932 708 226 162 152
AseI AT'TA_AT
Cuts at: 160 1032 4540 160
Size: 872 3508 1606
Fragments arranged by size:
3508 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5986
AvaI C'yCGr_G
Cuts at: 1870 1943 3311 1870
Size: 73 1368 4545
Fragments arranged by size:
4545 1368 73
AvrII C'CTAG_G
Cuts at: 2998 2998
Size: 5986
BamHI G'GATC_C
Cuts at: 3316 3683 3316
Size: 367 5619
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3307 4762 618
Size: 325 217 627 1520 1455 1842
Fragments arranged by size:
1842 1520 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 3075 3236 3305 1391
Size: 1684 161 69 4072
Fragments arranged by size:
4072 1684 161 69
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5015
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4177 4211 1903
Size: 34 2240 34 3678
Fragments arranged by size:
3678 2240 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2781 3878 5088 2781
Size: 1097 1210 3679
Fragments arranged by size:
3679 1210 1097
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5986
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4597 136
Size: 107 122 71 631 605 2925 1525
Fragments arranged by size:
2925 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2637 5981 2637
Size: 3344 2642
BlpI GC'TnA_GC
Cuts at: 3213 3403 3213
Size: 190 5796
Bme1580I G_kGCm'C
Cuts at: 1790 2783 4047 5203 1790
Size: 993 1264 1156 2573
Fragments arranged by size:
2573 1264 1156 993
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5986
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3717 4675 442
Size: 416 53 83 399 24 2300 958 1753
Fragments arranged by size:
2300 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5986
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2231 2933 2993 3170 3206 3264 4646 1239
Size: 992 702 60 177 36 58 1382 2579
Fragments arranged by size:
2579 1382 992 702 177 60 58 36
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4111 4976 5274 4111
Size: 865 298 4823
Fragments arranged by size:
4823 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4649 882
Size: 617 3150 2219
Fragments arranged by size:
3150 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3777 493
Size: 711 2573 2702
Fragments arranged by size:
2702 2573 711
BsaBI GATnn'nnATC
Cuts at: 5980 5980
Size: 5986
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3793 4175 4810 275
Size: 53 83 186 936 2260 382 635 1451
Fragments arranged by size:
2260 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4419 4817 5091 5238 5649 4419
Size: 398 274 147 411 4756
Fragments arranged by size:
4756 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2423 2453 1178
Size: 30 165 30 1020 30 4711
Fragments arranged by size:
4711 1020 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2930 3038 1186
Size: 1744 108 4134
Fragments arranged by size:
4134 1744 108
BseYI C'CCAG_C
Cuts at: 5189 5685 5189
Size: 496 5490
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2807 3080 3110 1163
Size: 573 1071 273 30 4039
Fragments arranged by size:
4039 1071 573 273 30
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2016 2301 3436 4197 5225 1540
Size: 275 186 15 285 1135 761 1028 2301
Fragments arranged by size:
2301 1135 1028 761 285 275 186 15
BsiHKAI G_wGCw'C
Cuts at: 3075 4047 4132 5203 3075
Size: 972 85 1071 3858
Fragments arranged by size:
3858 1071 972 85
BsmI GAATG_Cn'
Cuts at: 3190 3497 3590 3190
Size: 307 93 5586
Fragments arranged by size:
5586 307 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2783 3075 3236 3305 4047 4132 5203
Size: 399 993 292 161 69 742 85 1071
Cuts at: 5203 1391
Size: 2174
Fragments arranged by size:
2174 1071 993 742 399 292 161 85
69
BspEI T'CCGG_A
Cuts at: 5649 5649
Size: 5986
BspHI T'CATG_A
Cuts at: 3875 5605 3875
Size: 1730 4256
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2400 2709 2779 3341 844
Size: 1556 309 70 562 3489
Fragments arranged by size:
3489 1556 562 309 70
BsrBI CCG'CTC
Cuts at: 1394 1823 3873 1394
Size: 429 2050 3507
Fragments arranged by size:
3507 2050 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2466 2550 4481 4655 65
Size: 1183 1218 84 1931 174 1396
Fragments arranged by size:
1931 1396 1218 1183 174 84
BsrFI r'CCGG_y
Cuts at: 1700 1810 4630 4817 1700
Size: 110 2820 187 2869
Fragments arranged by size:
2869 2820 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5986
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5986
BssSI C'ACGA_G
Cuts at: 3098 4046 5058 3098
Size: 948 1012 4026
Fragments arranged by size:
4026 1012 948
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5986
BstBI TT'CG_AA
Cuts at: 2205 2573 4790 2205
Size: 368 2217 3401
Fragments arranged by size:
3401 2217 368
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5986
BstXI CCAn_nnnn'nTGG
Cuts at: 2179 2179
Size: 5986
BstYI r'GATC_y
Cuts at: 1972 2409 2637 2857 3316 3683 4068 4085 5537
Size: 437 228 220 459 367 385 17 1452
Cuts at: 5537 5623 5635 5981 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 459 437 385 367 346 228
220 86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2475 513
Size: 552 939 471 4024
Fragments arranged by size:
4024 939 552 471
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5247
Fragments arranged by size:
5247 683 56
BtsI GCAGTG_nn'
Cuts at: 2806 3502 4296 4316 2806
Size: 696 794 20 4476
Fragments arranged by size:
4476 794 696 20
ClaI AT'CG_AT
Cuts at: 1554 2370 1554
Size: 816 5170
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5951
DraI TTT'AAA
Cuts at: 3290 3290
Size: 5986
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4993 809
Size: 507 3677 1802
Fragments arranged by size:
3677 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3433 4322 5951
Size: 54 1425 50 275 18 1603 889 1629
Cuts at: 5951 8
Size: 43
Fragments arranged by size:
1629 1603 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3433 1537
Size: 275 1621 4090
Fragments arranged by size:
4090 1621 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2394 2410 2570 3015 3257 3916 1353
Size: 1041 16 160 445 242 659 3423
Fragments arranged by size:
3423 1041 659 445 242 160 16
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4573 4947 5093 387
Size: 1064 138 2984 374 146 1280
Fragments arranged by size:
2984 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 3073 3073
Size: 5986
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2231 2510 2756 2933 2941 2993 3137
Size: 393 599 279 246 177 8 52 144
Cuts at: 3137 3170 3206 3264 3281 4049 4646 5433 5701
Size: 33 36 58 17 768 597 787 268
Cuts at: 5701 1239
Size: 1524
Fragments arranged by size:
1524 787 768 599 597 393 279 268
246 177 144 58 52 36 33 17
8
EcoRI G'AATT_C
Cuts at: 2363 3295 3673 2363
Size: 932 378 4676
Fragments arranged by size:
4676 932 378
FspI TGC'GCA
Cuts at: 4492 4492
Size: 5986
HaeII r_GCGC'y
Cuts at: 1678 5133 1678
Size: 3455 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2622 2648 2654 2680 4627
Size: 32 135 32 1050 26 6 26 1947
Cuts at: 4627 4659 4701 4733 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1947 1050 135 42 32 32 32
32 26 26 6
HincII GTy'rAC
Cuts at: 669 1657 1938 2848 3330 3576 669
Size: 988 281 910 482 246 3079
Fragments arranged by size:
3079 988 910 482 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5986
HpaI GTT'AAC
Cuts at: 3576 3576
Size: 5986
KpnI G_GTAC'C
Cuts at: 3311 3311
Size: 5986
MfeI C'AATT_G
Cuts at: 3585 3585
Size: 5986
MluI A'CGCG_T
Cuts at: 5862 5862
Size: 5986
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5100 5284 1305
Size: 3795 184 2007
Fragments arranged by size:
3795 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4164
Fragments arranged by size:
4164 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2177 3944 4303 4462 518
Size: 648 1011 1767 359 159 2042
Fragments arranged by size:
2042 1767 1011 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2098 2904 3402 4079 5227 5472 1606
Size: 492 806 498 677 1148 245 2120
Fragments arranged by size:
2120 1148 806 677 498 492 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4495
Fragments arranged by size:
4495 939 552
NdeI CA'TA_TG
Cuts at: 387 2974 387
Size: 2587 3399
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5986
NotI GC'GGCC_GC
Cuts at: 3433 3433
Size: 5986
NspI r_CATG'y
Cuts at: 1132 1229 2072 3344 4889 1132
Size: 97 843 1272 1545 2229
Fragments arranged by size:
2229 1545 1272 843 97
PasI CC'CwG_GG
Cuts at: 1348 3229 1348
Size: 1881 4105
PciI A'CATG_T
Cuts at: 2068 4885 2068
Size: 2817 3169
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5986
PmeI GTTT'AAAC
Cuts at: 3290 3290
Size: 5986
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4055 4087 5568 5600 4055
Size: 32 1481 32 4441
Fragments arranged by size:
4441 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5986
PsiI TTA'TAA
Cuts at: 3556 3556
Size: 5986
PstI C_TGCA'G
Cuts at: 830 2397 3338 830
Size: 1567 941 3478
Fragments arranged by size:
3478 1567 941
PvuI CG_AT'CG
Cuts at: 2001 2301 2001
Size: 300 5686
PvuII CAG'CTG
Cuts at: 2098 2098
Size: 5986
SacI G_AGCT'C
Cuts at: 3075 3075
Size: 5986
SalI G'TCGA_C
Cuts at: 1936 3328 1936
Size: 1392 4594
SapI GCTCTTCn'nnn_
Cuts at: 2394 2394
Size: 5986
SbfI CC_TGCA'GG
Cuts at: 2397 3338 2397
Size: 941 5045
ScaI AGT'ACT
Cuts at: 4234 5946 4234
Size: 1712 4274
SfcI C'TryA_G
Cuts at: 826 1045 2393 2652 3334 4469 5150 5341 826
Size: 219 1348 259 682 1135 681 191 1471
Fragments arranged by size:
1471 1348 1135 682 681 259 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5986
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5986
SmaI CCC'GGG
Cuts at: 1872 3313 1872
Size: 1441 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4090 4991 5253 820
Size: 197 926 2147 901 262 1553
Fragments arranged by size:
2147 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3777 493
Size: 3284 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5986
SphI G_CATG'C
Cuts at: 1132 3344 1132
Size: 2212 3774
SspI AAT'ATT
Cuts at: 5 52 3910 5
Size: 47 3858 2081
Fragments arranged by size:
3858 2081 47
StuI AGG'CCT
Cuts at: 1517 2387 1517
Size: 870 5116
StyI C'CwwG_G
Cuts at: 513 1065 2004 2774 2998 3425 513
Size: 552 939 770 224 427 3074
Fragments arranged by size:
3074 939 770 552 427 224
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2024 2030 2237 4013 4030 4183 4368
Size: 1255 444 6 207 1776 17 153 185
Cuts at: 4368 4813 325
Size: 445 1498
Fragments arranged by size:
1776 1498 1255 445 444 207 185 153
17 6
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4232 5944 96
Size: 274 80 33 51 1443 2255 1712 138
Fragments arranged by size:
2255 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5946 5978 5946
Size: 32 5954
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5986
XbaI T'CTAG_A
Cuts at: 3322 3322
Size: 5986
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5986
XmaI C'CCGG_G
Cuts at: 1870 3311 1870
Size: 1441 4545
XmnI GAAnn'nnTTC
Cuts at: 2993 3188 4115 2993
Size: 195 927 4864
Fragments arranged by size:
4864 927 195
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3794 276
Size: 53 83 186 936 2260 2468
Fragments arranged by size:
2468 2260 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI
AseI AsiSI AvaI AvrII BamHI BanI BanII BbsI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoRI FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NcoI NdeI NheI NotI NspI PasI
PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI
PvuII SacI SalI SapI SbfI ScaI SfcI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AfeI ApaI AscI BaeI BbeI BbvCI BfrBI BplI
Bpu10I BsiWI BsmBI BstZ17I Bsu36I DraIII EcoNI EcoO109I
EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV
NruI NsiI PacI PfoI PmlI PpuMI PspOMI PspXI
PsrI RsrII SacII SanDI SexAI SfiI SfoI SrfI
SwaI XcmI