Restriction map for
pFN21AA0121
(Circular) (Six-base) MAPSORT of: pFN21AA0121.seq Check: 1259 from: 1 to: 5575
pFN21AA0121 5575 bp
With 182 enzymes: SgfI *
February 27, 2009 14:31 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3385 278
Size: 53 83 186 936 1849 2468
Fragments arranged by size:
2468 1849 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2918 1937
Size: 981 4594
Acc65I G'GTAC_C
Cuts at: 2896 2896
Size: 5575
AclI AA'CG_TT
Cuts at: 1563 3702 4075 1563
Size: 2139 373 3063
Fragments arranged by size:
3063 2139 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3638 5022 5290 1632
Size: 2006 1384 268 1917
Fragments arranged by size:
2006 1917 1384 268
AfeI AGC'GCT
Cuts at: 2605 2605
Size: 5575
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5378
AflIII A'CryG_T
Cuts at: 1203 2745 4474 5451 1203
Size: 1542 1729 977 1327
Fragments arranged by size:
1729 1542 1327 977
AgeI A'CCGG_T
Cuts at: 4406 4406
Size: 5575
AhdI GACnn_n'nnGTC
Cuts at: 4304 4304
Size: 5575
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5575
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5575
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2474 2506 2474
Size: 32 5543
AlwNI CAG_nnn'CTG
Cuts at: 2107 2562 3318 4890 2107
Size: 455 756 1572 2792
Fragments arranged by size:
2792 1572 756 455
ApaLI G'TGCA_C
Cuts at: 3632 4788 3632
Size: 1156 4419
ApoI r'AATT_y
Cuts at: 1493 2884 3110 3262 5371 1493
Size: 1391 226 152 2109 1697
Fragments arranged by size:
2109 1697 1391 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4129 160
Size: 872 3097 1606
Fragments arranged by size:
3097 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5575
AvaI C'yCGr_G
Cuts at: 1870 1943 2295 2464 2573 2675 2900 1870
Size: 73 352 169 109 102 225 4545
Fragments arranged by size:
4545 352 225 169 109 102 73
BamHI G'GATC_C
Cuts at: 2905 3272 2905
Size: 367 5208
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2516 2683 2896 4351 618
Size: 325 217 627 729 167 213 1455 1842
Fragments arranged by size:
1842 1455 729 627 325 217 213 167
BanII G_rGCy'C
Cuts at: 1391 2676 2711 2894 1391
Size: 1285 35 183 4072
Fragments arranged by size:
4072 1285 183 35
BbeI G_GCGC'C
Cuts at: 2520 2520
Size: 5575
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4604
BbvCI CC'TCA_GC
Cuts at: 2126 2126
Size: 5575
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2299 2333 2708 2742 3766 3800 1903
Size: 34 362 34 375 34 1024 34 3678
Fragments arranged by size:
3678 1024 375 362 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3467 4677 3467
Size: 1210 4365
BclI T'GATC_A
Cuts at: 1548 2496 1548
Size: 948 4627
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2352 4186 136
Size: 107 122 71 631 605 680 1834 1525
Fragments arranged by size:
1834 1525 680 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5570 5570
Size: 5575
BlpI GC'TnA_GC
Cuts at: 2992 2992
Size: 5575
Bme1580I G_kGCm'C
Cuts at: 1790 3636 4792 1790
Size: 1846 1156 2573
Fragments arranged by size:
2573 1846 1156
BmgBI CAC'GTC
Cuts at: 1318 2224 1318
Size: 906 4669
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3306 4264 442
Size: 416 53 83 399 24 1889 958 1753
Fragments arranged by size:
1889 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5575
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2818 2818
Size: 5575
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2411 2738 4235 1239
Size: 1172 327 1497 2579
Fragments arranged by size:
2579 1497 1172 327
Bpu10I CC'TnA_GC
Cuts at: 2126 2126
Size: 5575
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3700 4565 4863 3700
Size: 865 298 4412
Fragments arranged by size:
4412 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2283 2875 4238 882
Size: 617 784 592 1363 2219
Fragments arranged by size:
2219 1363 784 617 592
BsaAI yAC'GTr
Cuts at: 493 1204 2748 3366 493
Size: 711 1544 618 2702
Fragments arranged by size:
2702 1544 711 618
BsaBI GATnn'nnATC
Cuts at: 5569 5569
Size: 5575
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2012 2427 2517 3382
Size: 53 83 186 936 479 415 90 865
Cuts at: 3382 3764 4399 275
Size: 382 635 1451
Fragments arranged by size:
1451 936 865 635 479 415 382 186
90 83 53
BsaWI w'CCGG_w
Cuts at: 2589 4008 4406 4680 4827 5238 2589
Size: 1419 398 274 147 411 2926
Fragments arranged by size:
2926 1419 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2131 2161 2210 2240 2474
Size: 30 165 30 728 30 49 30 234
Cuts at: 2474 2504 2840 2870 1178
Size: 30 336 30 3883
Fragments arranged by size:
3883 728 336 234 165 49 30 30
30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2648 1186
Size: 1462 4113
BseYI C'CCAG_C
Cuts at: 2162 2378 4778 5274 2162
Size: 216 2400 496 2463
Fragments arranged by size:
2463 2400 496 216
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2333 1163
Size: 573 597 4405
Fragments arranged by size:
4405 597 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3025 3786 4814 1540
Size: 275 186 1024 761 1028 2301
Fragments arranged by size:
2301 1028 1024 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3636 3721 4792 3636
Size: 85 1071 4419
Fragments arranged by size:
4419 1071 85
BsmI GAATG_Cn'
Cuts at: 2103 3086 3179 2103
Size: 983 93 4499
Fragments arranged by size:
4499 983 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2003 2230 2003
Size: 227 5348
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2676 2711 2894 3636 3721 4792 1391
Size: 399 886 35 183 742 85 1071 2174
Fragments arranged by size:
2174 1071 886 742 399 183 85 35
BspEI T'CCGG_A
Cuts at: 5238 5238
Size: 5575
BspHI T'CATG_A
Cuts at: 3464 5194 3464
Size: 1730 3845
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2930 844
Size: 2086 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2302 2473 3462 1394
Size: 429 479 171 989 3507
Fragments arranged by size:
3507 989 479 429 171
BsrDI GCAATG_nn'
Cuts at: 65 1248 4070 4244 65
Size: 1183 2822 174 1396
Fragments arranged by size:
2822 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2110 2141 2441 2484 2680 4219 4406
Size: 110 300 31 300 43 196 1539 187
Cuts at: 4406 1700
Size: 2869
Fragments arranged by size:
2869 1539 300 300 196 187 110 43
31
BsrGI T'GTAC_A
Cuts at: 96 2364 96
Size: 2268 3307
BssHII G'CGCG_C
Cuts at: 1925 2323 1925
Size: 398 5177
BssSI C'ACGA_G
Cuts at: 3635 4647 3635
Size: 1012 4563
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2107 794
Size: 1313 4262
BstBI TT'CG_AA
Cuts at: 4379 4379
Size: 5575
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5575
BstXI CCAn_nnnn'nTGG
Cuts at: 2737 2737
Size: 5575
BstYI r'GATC_y
Cuts at: 1972 2905 3272 3657 3674 5126 5212 5224 5570
Size: 933 367 385 17 1452 86 12 346
Cuts at: 5570 1972
Size: 1977
Fragments arranged by size:
1977 1452 933 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2625 2712 513
Size: 552 939 621 87 3376
Fragments arranged by size:
3376 939 621 552 87
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2533 487
Size: 683 56 1307 3529
Fragments arranged by size:
3529 1307 683 56
BtsI GCAGTG_nn'
Cuts at: 2539 3091 3885 3905 2539
Size: 552 794 20 4209
Fragments arranged by size:
4209 794 552 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5575
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5540
DraI TTT'AAA
Cuts at: 2879 2879
Size: 5575
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4582 809
Size: 507 3266 1802
Fragments arranged by size:
3266 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2766 3022 3911
Size: 54 1425 50 275 18 936 256 889
Cuts at: 3911 5540 8
Size: 1629 43
Fragments arranged by size:
1629 1425 936 889 275 256 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3022 1537
Size: 275 1210 4090
Fragments arranged by size:
4090 1210 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3505 1353
Size: 2152 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2788 4162 4536 4682 387
Size: 1064 138 1199 1374 374 146 1280
Fragments arranged by size:
1374 1280 1199 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2411 2738 3638 4235 5022 5290 1239
Size: 393 779 327 900 597 787 268 1524
Fragments arranged by size:
1524 900 787 779 597 393 327 268
EcoNI CCTnn'n_nnAGG
Cuts at: 2806 2806
Size: 5575
EcoO109I rG'GnC_Cy
Cuts at: 2200 2200
Size: 5575
EcoRI G'AATT_C
Cuts at: 2884 3262 2884
Size: 378 5197
FspI TGC'GCA
Cuts at: 4081 4081
Size: 5575
HaeII r_GCGC'y
Cuts at: 1678 2520 2607 4722 1678
Size: 842 87 2115 2531
Fragments arranged by size:
2531 2115 842 87
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2012 2044 2210 2242 2765
Size: 32 135 32 440 32 166 32 523
Cuts at: 2765 2797 4216 4248 4290 4322 1373
Size: 32 1419 32 42 32 2626
Fragments arranged by size:
2626 1419 523 440 166 135 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2919 3165 669
Size: 988 281 981 246 3079
Fragments arranged by size:
3079 988 981 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5575
HpaI GTT'AAC
Cuts at: 3165 3165
Size: 5575
KasI G'GCGC_C
Cuts at: 2516 2516
Size: 5575
KpnI G_GTAC'C
Cuts at: 2900 2900
Size: 5575
MfeI C'AATT_G
Cuts at: 3174 3174
Size: 5575
MluI A'CGCG_T
Cuts at: 5451 5451
Size: 5575
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4689 4873 1305
Size: 3384 184 2007
Fragments arranged by size:
3384 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3753
Fragments arranged by size:
3753 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2055 3533 3892 4051 518
Size: 648 889 1478 359 159 2042
Fragments arranged by size:
2042 1478 889 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2559 2991 3668 4816 5061 1606
Size: 953 432 677 1148 245 2120
Fragments arranged by size:
2120 1148 953 677 432 245
NaeI GCC'GGC
Cuts at: 2443 2682 2443
Size: 239 5336
NarI GG'CG_CC
Cuts at: 2517 2517
Size: 5575
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4084
Fragments arranged by size:
4084 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5575
NgoMIV G'CCGG_C
Cuts at: 2441 2680 2441
Size: 239 5336
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5575
NotI GC'GGCC_GC
Cuts at: 3022 3022
Size: 5575
NspI r_CATG'y
Cuts at: 1132 1229 2054 2933 4478 1132
Size: 97 825 879 1545 2229
Fragments arranged by size:
2229 1545 879 825 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5575
PciI A'CATG_T
Cuts at: 4474 4474
Size: 5575
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5575
PmeI GTTT'AAAC
Cuts at: 2879 2879
Size: 5575
PmlI CAC'GTG
Cuts at: 2748 2748
Size: 5575
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2474 2506 3644 3676 5157 5189 2474
Size: 32 1138 32 1481 32 2860
Fragments arranged by size:
2860 1481 1138 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2200 2200
Size: 5575
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5575
PsiI TTA'TAA
Cuts at: 3145 3145
Size: 5575
PstI C_TGCA'G
Cuts at: 830 2927 830
Size: 2097 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5575
SalI G'TCGA_C
Cuts at: 1936 2917 1936
Size: 981 4594
SbfI CC_TGCA'GG
Cuts at: 2927 2927
Size: 5575
ScaI AGT'ACT
Cuts at: 3823 5535 3823
Size: 1712 3863
SfcI C'TryA_G
Cuts at: 826 1045 2923 4058 4739 4930 826
Size: 219 1878 1135 681 191 1471
Fragments arranged by size:
1878 1471 1135 681 219 191
SfoI GGC'GCC
Cuts at: 2518 2518
Size: 5575
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5575
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5575
SmaI CCC'GGG
Cuts at: 1872 2297 2466 2575 2902 1872
Size: 425 169 109 327 4545
Fragments arranged by size:
4545 425 327 169 109
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3679 4580 4842 820
Size: 197 926 1736 901 262 1553
Fragments arranged by size:
1736 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3366 493
Size: 2873 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5575
SphI G_CATG'C
Cuts at: 1132 2054 2933 1132
Size: 922 879 3774
Fragments arranged by size:
3774 922 879
SrfI GCCC'GGGC
Cuts at: 2575 2575
Size: 5575
SspI AAT'ATT
Cuts at: 5 52 3499 5
Size: 47 3447 2081
Fragments arranged by size:
3447 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5575
StyI C'CwwG_G
Cuts at: 513 1065 2004 2383 2769 3014 513
Size: 552 939 379 386 245 3074
Fragments arranged by size:
3074 939 552 386 379 245
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2701 2729 3602 3619 3772 3957 4402
Size: 1255 1121 28 873 17 153 185 445
Cuts at: 4402 325
Size: 1498
Fragments arranged by size:
1498 1255 1121 873 445 185 153 28
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2364 3821 5533
Size: 274 80 33 51 1443 387 1457 1712
Cuts at: 5533 96
Size: 138
Fragments arranged by size:
1712 1457 1443 387 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2131 2163 2840 2872 5535 5567 2131
Size: 32 677 32 2663 32 2139
Fragments arranged by size:
2663 2139 677 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2222 1316
Size: 906 4669
XbaI T'CTAG_A
Cuts at: 2911 2911
Size: 5575
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2386 2419 2386
Size: 33 5542
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5575
XmaI C'CCGG_G
Cuts at: 1870 2295 2464 2573 2900 1870
Size: 425 169 109 327 4545
Fragments arranged by size:
4545 425 327 169 109
XmnI GAAnn'nnTTC
Cuts at: 3704 3704
Size: 5575
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3383 276
Size: 53 83 186 936 1849 2468
Fragments arranged by size:
2468 1849 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI
DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI
EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI
KasI KpnI MfeI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI
PasI PciI PflMI PmeI PmlI PpiI PpuMI PshAI
PsiI PstI PvuI SalI SbfI ScaI SfcI SfoI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI ApaI AscI AvrII BaeI BfrBI BsiWI BstZ17I
Bsu36I DraIII EcoICRI EcoRV FalI FseI FspAI NruI
NsiI PacI PfoI PspOMI PspXI PsrI PvuII RsrII
SacI SacII SanDI SapI SexAI SfiI SwaI