Restriction map for
pFN21AA0090
(Circular) (Six-base) MAPSORT of: pFN21AA0090.seq Check: 6992 from: 1 to: 7681
pFN21AA0090 7681 bp
With 182 enzymes: SgfI *
February 27, 2009 14:31 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 4590 4590
Size: 7681
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5491 278
Size: 53 83 186 936 3955 2468
Fragments arranged by size:
3955 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2070 4842 5024 1937
Size: 133 2772 182 4594
Fragments arranged by size:
4594 2772 182 133
Acc65I G'GTAC_C
Cuts at: 5002 5002
Size: 7681
AclI AA'CG_TT
Cuts at: 1563 5808 6181 1563
Size: 4245 373 3063
Fragments arranged by size:
4245 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2462 2569 3066 3878 4450 4889 5744 7128
Size: 830 107 497 812 572 439 855 1384
Cuts at: 7128 7396 1632
Size: 268 1917
Fragments arranged by size:
1917 1384 855 830 812 572 497 439
268 107
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 7484
AflIII A'CryG_T
Cuts at: 1203 4887 6580 7557 1203
Size: 3684 1693 977 1327
Fragments arranged by size:
3684 1693 1327 977
AgeI A'CCGG_T
Cuts at: 6512 6512
Size: 7681
AhdI GACnn_n'nnGTC
Cuts at: 3784 6410 3784
Size: 2626 5055
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 7681
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 7681
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2616 2648 2616
Size: 32 7649
AlwNI CAG_nnn'CTG
Cuts at: 2671 5424 6996 2671
Size: 2753 1572 3356
Fragments arranged by size:
3356 2753 1572
ApaI G_GGCC'C
Cuts at: 2569 2872 2569
Size: 303 7378
ApaLI G'TGCA_C
Cuts at: 3760 5738 6894 3760
Size: 1978 1156 4547
Fragments arranged by size:
4547 1978 1156
ApoI r'AATT_y
Cuts at: 1493 2141 2203 2804 3416 3587 4990 5216 5368
Size: 648 62 601 612 171 1403 226 152
Cuts at: 5368 7477 1493
Size: 2109 1697
Fragments arranged by size:
2109 1697 1403 648 612 601 226 171
152 62
AseI AT'TA_AT
Cuts at: 160 1032 6235 160
Size: 872 5203 1606
Fragments arranged by size:
5203 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7681
AvaI C'yCGr_G
Cuts at: 1870 1943 2722 2826 2865 4649 4780 4836 5006
Size: 73 779 104 39 1784 131 56 170
Cuts at: 5006 1870
Size: 4545
Fragments arranged by size:
4545 1784 779 170 131 104 73 56
39
BamHI G'GATC_C
Cuts at: 2153 4636 5011 5378 2153
Size: 2483 375 367 4456
Fragments arranged by size:
4456 2483 375 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2229 2481 3659 5002 6457
Size: 325 217 627 442 252 1178 1343 1455
Cuts at: 6457 618
Size: 1842
Fragments arranged by size:
1842 1455 1343 1178 627 442 325 252
217
BanII G_rGCy'C
Cuts at: 1391 2569 2729 2872 4431 5000 1391
Size: 1178 160 143 1559 569 4072
Fragments arranged by size:
4072 1559 1178 569 160 143
BbeI G_GCGC'C
Cuts at: 2233 2233
Size: 7681
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2082 2453 2999 3802 928
Size: 971 183 371 546 803 4807
Fragments arranged by size:
4807 971 803 546 371 183
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5872 5906 1903
Size: 34 3935 34 3678
Fragments arranged by size:
3935 3678 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 5573 6783 5573
Size: 1210 6471
BclI T'GATC_A
Cuts at: 1548 2295 4932 1548
Size: 747 2637 4297
Fragments arranged by size:
4297 2637 747
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2009 3211 4544
Size: 107 122 71 631 605 337 1202 1333
Cuts at: 4544 6292 136
Size: 1748 1525
Fragments arranged by size:
1748 1525 1333 1202 631 605 337 122
107 71
BglII A'GATC_T
Cuts at: 2220 7676 2220
Size: 5456 2225
BlpI GC'TnA_GC
Cuts at: 5098 5098
Size: 7681
Bme1580I G_kGCm'C
Cuts at: 1790 2249 2484 2569 2872 3397 3764 4442 5742
Size: 459 235 85 303 525 367 678 1300
Cuts at: 5742 6898 1790
Size: 1156 2573
Fragments arranged by size:
2573 1300 1156 678 525 459 367 303
235 85
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 7681
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2223 2368 2846
Size: 416 53 83 399 24 806 145 478
Cuts at: 2846 2866 2994 3399 3845 3884 4308 4378 5412
Size: 20 128 405 446 39 424 70 1034
Cuts at: 5412 6370 442
Size: 958 1753
Fragments arranged by size:
1753 1034 958 806 478 446 424 416
405 399 145 128 83 70 53 39
24 20
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7681
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3216 3670 3216
Size: 454 7227
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2458 2697 2774 3581 4445 4487 4623 4683
Size: 1219 239 77 807 864 42 136 60
Cuts at: 4683 4808 6341 1239
Size: 125 1533 2579
Fragments arranged by size:
2579 1533 1219 864 807 239 136 125
77 60 42
Bpu10I CC'TnA_GC
Cuts at: 3207 3207
Size: 7681
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2471 5806 6671 6969 2471
Size: 3335 865 298 3183
Fragments arranged by size:
3335 3183 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 6344 882
Size: 617 4845 2219
Fragments arranged by size:
4845 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 4888 5472 493
Size: 711 3684 584 2702
Fragments arranged by size:
3684 2702 711 584
BsaBI GATnn'nnATC
Cuts at: 7675 7675
Size: 7681
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2230 4293 5488 5870
Size: 53 83 186 936 697 2063 1195 382
Cuts at: 5870 6505 275
Size: 635 1451
Fragments arranged by size:
2063 1451 1195 936 697 635 382 186
83 53
BsaWI w'CCGG_w
Cuts at: 6114 6512 6786 6933 7344 6114
Size: 398 274 147 411 6451
Fragments arranged by size:
6451 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2210 2240 2445 2475 2616
Size: 30 165 30 807 30 205 30 141
Cuts at: 2616 2646 2721 2751 1178
Size: 30 75 30 6108
Fragments arranged by size:
6108 807 205 165 141 75 30 30
30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3353 3694 4306 4691 1186
Size: 2167 341 612 385 4176
Fragments arranged by size:
4176 2167 612 385 341
BseYI C'CCAG_C
Cuts at: 2841 3442 4485 6884 7380 2841
Size: 601 1043 2399 496 3142
Fragments arranged by size:
3142 2399 1043 601 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2264 2867 3290 4343 1163
Size: 573 528 603 423 1053 4501
Fragments arranged by size:
4501 1053 603 573 528 423
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2064 5131 5892 6920 1540
Size: 275 186 63 3067 761 1028 2301
Fragments arranged by size:
3067 2301 1028 761 275 186 63
BsiHKAI G_wGCw'C
Cuts at: 2729 3764 4155 4197 4244 4431 5742 5827 6898
Size: 1035 391 42 47 187 1311 85 1071
Cuts at: 6898 2729
Size: 3512
Fragments arranged by size:
3512 1311 1071 1035 391 187 85 47
42
BsmI GAATG_Cn'
Cuts at: 4357 4761 5192 5285 4357
Size: 404 431 93 6753
Fragments arranged by size:
6753 431 404 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2750 3474 2750
Size: 724 6957
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2249 2484 2569 2729 2872 3397 3764
Size: 399 459 235 85 160 143 525 367
Cuts at: 3764 4155 4197 4244 4431 4442 5000 5742 5827
Size: 391 42 47 187 11 558 742 85
Cuts at: 5827 6898 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 742 558 525 459 399 391
367 235 187 160 143 85 85 47
42 11
BspEI T'CCGG_A
Cuts at: 7344 7344
Size: 7681
BspHI T'CATG_A
Cuts at: 5570 7300 5570
Size: 1730 5951
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2629 4227 4590 5036 844
Size: 1785 1598 363 446 3489
Fragments arranged by size:
3489 1785 1598 446 363
BsrBI CCG'CTC
Cuts at: 1394 1823 3195 3211 5568 1394
Size: 429 1372 16 2357 3507
Fragments arranged by size:
3507 2357 1372 429 16
BsrDI GCAATG_nn'
Cuts at: 65 1248 6176 6350 65
Size: 1183 4928 174 1396
Fragments arranged by size:
4928 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 6325 6512 1700
Size: 110 4515 187 2869
Fragments arranged by size:
4515 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 4705 96
Size: 4609 3072
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7681
BssSI C'ACGA_G
Cuts at: 3137 5741 6753 3137
Size: 2604 1012 4065
Fragments arranged by size:
4065 2604 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3880 794
Size: 3086 4595
BstBI TT'CG_AA
Cuts at: 4060 6485 4060
Size: 2425 5256
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 7681
BstXI CCAn_nnnn'nTGG
Cuts at: 2164 2396 2854 4222 4801 2164
Size: 232 458 1368 579 5044
Fragments arranged by size:
5044 1368 579 458 232
BstYI r'GATC_y
Cuts at: 1972 2153 2220 2778 4066 4636 4653 5011 5378
Size: 181 67 558 1288 570 17 358 367
Cuts at: 5378 5763 5780 7232 7318 7330 7676 1972
Size: 385 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 1288 570 558 385 367 358
346 181 86 67 17 17 12
Bsu36I CC'TnA_GG
Cuts at: 3625 4623 3625
Size: 998 6683
BtgI C'CryG_G
Cuts at: 513 1065 2004 2064 2657 4227 4539 513
Size: 552 939 60 593 1570 312 3655
Fragments arranged by size:
3655 1570 939 593 552 312 60
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2416 487
Size: 683 56 1190 5752
Fragments arranged by size:
5752 1190 683 56
BtsI GCAGTG_nn'
Cuts at: 2783 4153 4195 5197 5991 6011 2783
Size: 1370 42 1002 794 20 4453
Fragments arranged by size:
4453 1370 1002 794 42 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7681
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 7646
DraI TTT'AAA
Cuts at: 3712 4985 3712
Size: 1273 6408
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 6688 809
Size: 507 5372 1802
Fragments arranged by size:
5372 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2061 3576 4224
Size: 54 1425 50 275 18 231 1515 648
Cuts at: 4224 5128 6017 7646 8
Size: 904 889 1629 43
Fragments arranged by size:
1629 1515 1425 904 889 648 275 231
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2061 5128 1537
Size: 275 249 3067 4090
Fragments arranged by size:
4090 3067 275 249
EarI CTCTTCn'nnn_
Cuts at: 1353 5611 1353
Size: 4258 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 6268 6642 6788 387
Size: 1064 138 4679 374 146 1280
Fragments arranged by size:
4679 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2727 4429 2727
Size: 1702 5979
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2458 2462 2569 2697 2774 3066 3581
Size: 393 826 4 107 128 77 292 515
Cuts at: 3581 3878 4445 4450 4487 4623 4683 4808 4889
Size: 297 567 5 37 136 60 125 81
Cuts at: 4889 5744 6341 7128 7396 1239
Size: 855 597 787 268 1524
Fragments arranged by size:
1524 855 826 787 597 567 515 393
297 292 268 136 128 125 107 81
77 60 37 5 4
EcoNI CCTnn'n_nnAGG
Cuts at: 4622 4622
Size: 7681
EcoO109I rG'GnC_Cy
Cuts at: 2350 2566 2830 4340 4498 2350
Size: 216 264 1510 158 5533
Fragments arranged by size:
5533 1510 264 216 158
EcoRI G'AATT_C
Cuts at: 3587 4990 5368 3587
Size: 1403 378 5900
Fragments arranged by size:
5900 1403 378
EcoRV GAT'ATC
Cuts at: 4052 4052
Size: 7681
FspI TGC'GCA
Cuts at: 6187 6187
Size: 7681
HaeII r_GCGC'y
Cuts at: 1678 2233 3865 6828 1678
Size: 555 1632 2963 2531
Fragments arranged by size:
2963 2531 1632 555
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2304 2336 2711 2743 3265
Size: 32 135 32 732 32 375 32 522
Cuts at: 3265 3297 3770 3802 4735 4767 6322 6354 6396
Size: 32 473 32 933 32 1555 32 42
Cuts at: 6396 6428 1373
Size: 32 2626
Fragments arranged by size:
2626 1555 933 732 522 473 375 135
42 32 32 32 32 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2239 5025 5271 669
Size: 988 281 301 2786 246 3079
Fragments arranged by size:
3079 2786 988 301 281 246
HindIII A'AGCT_T
Cuts at: 748 3632 748
Size: 2884 4797
HpaI GTT'AAC
Cuts at: 5271 5271
Size: 7681
KasI G'GCGC_C
Cuts at: 2229 2229
Size: 7681
KpnI G_GTAC'C
Cuts at: 5006 5006
Size: 7681
MfeI C'AATT_G
Cuts at: 5280 5280
Size: 7681
MluI A'CGCG_T
Cuts at: 7557 7557
Size: 7681
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2845 2999 6795 6979 1305
Size: 1540 154 3796 184 2007
Fragments arranged by size:
3796 2007 1540 184 154
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3578 10
Size: 54 1425 343 1746 4113
Fragments arranged by size:
4113 1746 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 5639 5998 6157 518
Size: 648 4473 359 159 2042
Fragments arranged by size:
4473 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2066 4117 4489 5097 5774 6922 7167 1606
Size: 460 2051 372 608 677 1148 245 2120
Fragments arranged by size:
2120 2051 1148 677 608 460 372 245
NarI GG'CG_CC
Cuts at: 2230 2230
Size: 7681
NcoI C'CATG_G
Cuts at: 513 1065 2004 4539 513
Size: 552 939 2535 3655
Fragments arranged by size:
3655 2535 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7681
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7681
NotI GC'GGCC_GC
Cuts at: 2061 5128 2061
Size: 3067 4614
NspI r_CATG'y
Cuts at: 1132 1229 5039 6584 1132
Size: 97 3810 1545 2229
Fragments arranged by size:
3810 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 4165 1348
Size: 2817 4864
PciI A'CATG_T
Cuts at: 6580 6580
Size: 7681
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2746 3669 1108
Size: 1638 923 5120
Fragments arranged by size:
5120 1638 923
PmeI GTTT'AAAC
Cuts at: 4985 4985
Size: 7681
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2616 2648 2716 2748 3721 3753 5750 5782 7263
Size: 32 68 32 973 32 1997 32 1481
Cuts at: 7263 7295 2616
Size: 32 3002
Fragments arranged by size:
3002 1997 1481 973 68 32 32 32
32 32
PpuMI rG'GwC_Cy
Cuts at: 2830 4498 2830
Size: 1668 6013
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7681
PsiI TTA'TAA
Cuts at: 5251 5251
Size: 7681
PspOMI G'GGCC_C
Cuts at: 2565 2868 2565
Size: 303 7378
PspXI vC'TCGA_Gb
Cuts at: 4836 4836
Size: 7681
PstI C_TGCA'G
Cuts at: 830 2415 2667 2790 2916 3007 3303 5033 830
Size: 1585 252 123 126 91 296 1730 3478
Fragments arranged by size:
3478 1730 1585 296 252 126 123 91
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7681
PvuII CAG'CTG
Cuts at: 4489 4489
Size: 7681
SacI G_AGCT'C
Cuts at: 2729 4431 2729
Size: 1702 5979
SacII CC_GC'GG
Cuts at: 2067 2067
Size: 7681
SalI G'TCGA_C
Cuts at: 1936 5023 1936
Size: 3087 4594
SbfI CC_TGCA'GG
Cuts at: 2415 5033 2415
Size: 2618 5063
ScaI AGT'ACT
Cuts at: 4385 5929 7641 4385
Size: 1544 1712 4425
Fragments arranged by size:
4425 1712 1544
SfcI C'TryA_G
Cuts at: 826 1045 2411 2663 2786 2912 3003 3299 5029
Size: 219 1366 252 123 126 91 296 1730
Cuts at: 5029 6164 6845 7036 826
Size: 1135 681 191 1471
Fragments arranged by size:
1730 1471 1366 1135 681 296 252 219
191 126 123 91
SfoI GGC'GCC
Cuts at: 2231 2231
Size: 7681
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7681
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7681
SmaI CCC'GGG
Cuts at: 1872 2828 2867 5008 1872
Size: 956 39 2141 4545
Fragments arranged by size:
4545 2141 956 39
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2486 4836 5785 6686 6948 820
Size: 197 926 543 2350 949 901 262 1553
Fragments arranged by size:
2350 1553 949 926 901 543 262 197
SnaBI TAC'GTA
Cuts at: 493 5472 493
Size: 4979 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7681
SphI G_CATG'C
Cuts at: 1132 5039 1132
Size: 3907 3774
SspI AAT'ATT
Cuts at: 5 52 5605 5
Size: 47 5553 2081
Fragments arranged by size:
5553 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 7681
StyI C'CwwG_G
Cuts at: 513 1065 2004 2136 3912 4395 4539 5120 513
Size: 552 939 132 1776 483 144 581 3074
Fragments arranged by size:
3074 1776 939 581 552 483 144 132
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2482 4360 5708 5725 5878 6063 6508
Size: 1255 902 1878 1348 17 153 185 445
Cuts at: 6508 325
Size: 1498
Fragments arranged by size:
1878 1498 1348 1255 902 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4111 4383 4705
Size: 274 80 33 51 1443 2134 272 322
Cuts at: 4705 5927 7639 96
Size: 1222 1712 138
Fragments arranged by size:
2134 1712 1443 1222 322 274 272 138
80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2210 2242 2277 2309 2445 2477 2721 2753 3940
Size: 32 35 32 136 32 244 32 1187
Cuts at: 3940 3972 4145 4177 7641 7673 2210
Size: 32 173 32 3464 32 2218
Fragments arranged by size:
3464 2218 1187 244 173 136 35 32
32 32 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 7681
XbaI T'CTAG_A
Cuts at: 5017 5017
Size: 7681
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 4537 4537
Size: 7681
XhoI C'TCGA_G
Cuts at: 1943 4836 1943
Size: 2893 4788
XmaI C'CCGG_G
Cuts at: 1870 2826 2865 5006 1870
Size: 956 39 2141 4545
Fragments arranged by size:
4545 2141 956 39
XmnI GAAnn'nnTTC
Cuts at: 3796 5810 3796
Size: 2014 5667
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5489 276
Size: 53 83 186 936 3955 2468
Fragments arranged by size:
3955 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI
CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI EcoRV FspI HaeII Hin4I
HincII HindIII HpaI KasI KpnI MfeI MluI MmeI
MscI MslI MspA1I NarI NcoI NdeI NheI NotI
NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI
PsiI PspOMI PspXI PstI PvuI PvuII SacI SacII
SalI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AfeI AscI AvrII BaeI BbvCI BfrBI BsiWI BstZ17I
DraIII FalI FseI FspAI NaeI NgoMIV NruI NsiI
PacI PfoI PmlI PsrI RsrII SanDI SapI SexAI
SfiI SrfI SwaI