Restriction map for
pFN21AA0082
(Circular) (Six-base) MAPSORT of: pFN21AA0082.seq Check: 6000 from: 1 to: 7210
pFN21AA0082 7210 bp
With 182 enzymes: SgfI *
January 31, 2011 17:48 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2455 2455
Size: 7210
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3435 4482 5020 278
Size: 53 83 186 936 1899 1047 538 2468
Fragments arranged by size:
2468 1899 1047 936 538 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3255 4553 1937
Size: 1318 1298 4594
Fragments arranged by size:
4594 1318 1298
Acc65I G'GTAC_C
Cuts at: 4531 4531
Size: 7210
AclI AA'CG_TT
Cuts at: 1563 5337 5710 1563
Size: 3774 373 3063
Fragments arranged by size:
3774 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2287 3371 3697 3905 5273 6657 6925 1632
Size: 655 1084 326 208 1368 1384 268 1917
Fragments arranged by size:
1917 1384 1368 1084 655 326 268 208
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 7013
AflIII A'CryG_T
Cuts at: 1203 6109 7086 1203
Size: 4906 977 1327
Fragments arranged by size:
4906 1327 977
AgeI A'CCGG_T
Cuts at: 6041 6041
Size: 7210
AhdI GACnn_n'nnGTC
Cuts at: 4465 5939 4465
Size: 1474 5736
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 7210
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3167 4445 1184
Size: 1983 1278 3949
Fragments arranged by size:
3949 1983 1278
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3410 3442 3410
Size: 32 7178
AlwNI CAG_nnn'CTG
Cuts at: 2419 4953 6525 2419
Size: 2534 1572 3104
Fragments arranged by size:
3104 2534 1572
ApaI G_GGCC'C
Cuts at: 2918 2918
Size: 7210
ApaLI G'TGCA_C
Cuts at: 5267 6423 5267
Size: 1156 6054
ApoI r'AATT_y
Cuts at: 1493 2496 3043 3476 4041 4058 4519 4745 4897
Size: 1003 547 433 565 17 461 226 152
Cuts at: 4897 7006 1493
Size: 2109 1697
Fragments arranged by size:
2109 1697 1003 565 547 461 433 226
152 17
AseI AT'TA_AT
Cuts at: 160 1032 5764 160
Size: 872 4732 1606
Fragments arranged by size:
4732 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7210
AvaI C'yCGr_G
Cuts at: 1870 1943 2661 3900 4535 1870
Size: 73 718 1239 635 4545
Fragments arranged by size:
4545 1239 718 635 73
BamHI G'GATC_C
Cuts at: 3624 4540 4907 3624
Size: 916 367 5927
Fragments arranged by size:
5927 916 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 4007 4190 4531 5986 618
Size: 325 217 627 2220 183 341 1455 1842
Fragments arranged by size:
2220 1842 1455 627 341 325 217 183
BanII G_rGCy'C
Cuts at: 1391 2451 2656 2918 4184 4275 4529 1391
Size: 1060 205 262 1266 91 254 4072
Fragments arranged by size:
4072 1266 1060 262 254 205 91
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2686 3893 928
Size: 971 787 1207 4245
Fragments arranged by size:
4245 1207 971 787
BbvCI CC'TCA_GC
Cuts at: 2084 3140 2084
Size: 1056 6154
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5401 5435 1903
Size: 34 3464 34 3678
Fragments arranged by size:
3678 3464 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 5102 6312 5102
Size: 1210 6000
BclI T'GATC_A
Cuts at: 1548 3459 1548
Size: 1911 5299
BfrBI ATG'CAT
Cuts at: 2213 2213
Size: 7210
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2847 4087 5821
Size: 107 122 71 631 605 1175 1240 1734
Cuts at: 5821 136
Size: 1525
Fragments arranged by size:
1734 1525 1240 1175 631 605 122 107
71
BglII A'GATC_T
Cuts at: 3834 7205 3834
Size: 3371 3839
BlpI GC'TnA_GC
Cuts at: 2808 4202 4627 2808
Size: 1394 425 5391
Fragments arranged by size:
5391 1394 425
Bme1580I G_kGCm'C
Cuts at: 1790 2918 3698 5271 6427 1790
Size: 1128 780 1573 1156 2573
Fragments arranged by size:
2573 1573 1156 1128 780
BmgBI CAC'GTC
Cuts at: 1318 2094 1318
Size: 776 6434
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2445 4941 5899
Size: 416 53 83 399 24 1028 2496 958
Cuts at: 5899 442
Size: 1753
Fragments arranged by size:
2496 1753 1028 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7210
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3046 3305 3597 3046
Size: 259 292 6659
Fragments arranged by size:
6659 292 259
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2954 3473 3770 4168 5870 1239
Size: 1715 519 297 398 1702 2579
Fragments arranged by size:
2579 1715 1702 519 398 297
Bpu10I CC'TnA_GC
Cuts at: 2078 2084 3140 2078
Size: 6 1056 6148
Fragments arranged by size:
6148 1056 6
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 5335 6200 6498 5335
Size: 865 298 6047
Fragments arranged by size:
6047 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2165 3462 5873 882
Size: 617 666 1297 2411 2219
Fragments arranged by size:
2411 2219 1297 666 617
BsaAI yAC'GTr
Cuts at: 493 1204 5001 493
Size: 711 3797 2702
Fragments arranged by size:
3797 2702 711
BsaBI GATnn'nnATC
Cuts at: 3707 4169 7204 3707
Size: 462 3035 3713
Fragments arranged by size:
3713 3035 462
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3432 4479 5017 5399
Size: 53 83 186 936 1899 1047 538 382
Cuts at: 5399 6034 275
Size: 635 1451
Fragments arranged by size:
1899 1451 1047 936 635 538 382 186
83 53
BsaWI w'CCGG_w
Cuts at: 3189 5643 6041 6315 6462 6873 3189
Size: 2454 398 274 147 411 3526
Fragments arranged by size:
3526 2454 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 4469 4499 1178
Size: 30 165 30 3066 30 3889
Fragments arranged by size:
3889 3066 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2026 2104 2703 1186
Size: 840 78 599 5693
Fragments arranged by size:
5693 840 599 78
BseYI C'CCAG_C
Cuts at: 6413 6909 6413
Size: 496 6714
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 6637
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4660 5421 6449 1540
Size: 275 186 2659 761 1028 2301
Fragments arranged by size:
2659 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2656 5271 5356 6427 2656
Size: 2615 85 1071 3439
Fragments arranged by size:
3439 2615 1071 85
BsmI GAATG_Cn'
Cuts at: 2033 2213 4422 4721 4814 2033
Size: 180 2209 299 93 4429
Fragments arranged by size:
4429 2209 299 180 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2278 2278
Size: 7210
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2451 2656 2918 3698 4184 4275 4529
Size: 399 661 205 262 780 486 91 254
Cuts at: 4529 5271 5356 6427 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1071 780 742 661 486 399 262
254 205 91 85
BspEI T'CCGG_A
Cuts at: 3189 6873 3189
Size: 3684 3526
BspHI T'CATG_A
Cuts at: 5099 6829 5099
Size: 1730 5480
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2455 3135 3267 4565 844
Size: 1611 680 132 1298 3489
Fragments arranged by size:
3489 1611 1298 680 132
BsrBI CCG'CTC
Cuts at: 1394 1823 5097 1394
Size: 429 3274 3507
Fragments arranged by size:
3507 3274 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2721 4365 5705 5879 65
Size: 1183 1473 1644 1340 174 1396
Fragments arranged by size:
1644 1473 1396 1340 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4224 4395 5854 6041 1700
Size: 110 2414 171 1459 187 2869
Fragments arranged by size:
2869 2414 1459 187 171 110
BsrGI T'GTAC_A
Cuts at: 96 4122 96
Size: 4026 3184
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7210
BssSI C'ACGA_G
Cuts at: 3639 5270 6282 3639
Size: 1631 1012 4567
Fragments arranged by size:
4567 1631 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2072 794
Size: 1278 5932
BstBI TT'CG_AA
Cuts at: 2967 6014 2967
Size: 3047 4163
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 7210
BstYI r'GATC_y
Cuts at: 1972 3135 3624 3756 3834 4540 4907 5292 5309
Size: 1163 489 132 78 706 367 385 17
Cuts at: 5309 6761 6847 6859 7205 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 1163 706 489 385 367 346
132 86 78 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2765 4239 513
Size: 552 1700 1474 3484
Fragments arranged by size:
3484 1700 1474 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6471
Fragments arranged by size:
6471 683 56
BtsI GCAGTG_nn'
Cuts at: 2031 2612 3396 3728 3802 4726 5520 5540 2031
Size: 581 784 332 74 924 794 20 3701
Fragments arranged by size:
3701 924 794 784 581 332 74 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7210
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 7175
DraI TTT'AAA
Cuts at: 4514 4514
Size: 7210
DraIII CAC_nnn'GTG
Cuts at: 2599 3575 2599
Size: 976 6234
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 6217 809
Size: 507 4901 1802
Fragments arranged by size:
4901 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2289 3850 4393
Size: 54 1425 50 275 18 459 1561 543
Cuts at: 4393 4657 5546 7175 8
Size: 264 889 1629 43
Fragments arranged by size:
1629 1561 1425 889 543 459 275 264
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4657 1537
Size: 275 2845 4090
Fragments arranged by size:
4090 2845 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2003 2374 2577 2634 2969 3296 3389 4128
Size: 650 371 203 57 335 327 93 739
Cuts at: 4128 4406 5140 1353
Size: 278 734 3423
Fragments arranged by size:
3423 739 734 650 371 335 327 278
203 93 57
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3749 5797 6171 6317 387
Size: 1064 138 2160 2048 374 146 1280
Fragments arranged by size:
2160 2048 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2654 2654
Size: 7210
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2287 2954 3371 3473 3697 3770 3905
Size: 393 655 667 417 102 224 73 135
Cuts at: 3905 4168 5273 5870 6657 6925 1239
Size: 263 1105 597 787 268 1524
Fragments arranged by size:
1524 1105 787 667 655 597 417 393
268 263 224 135 102 73
EcoO109I rG'GnC_Cy
Cuts at: 2914 2915 3346 2914
Size: 1 431 6778
Fragments arranged by size:
6778 431 1
EcoRI G'AATT_C
Cuts at: 4041 4519 4897 4041
Size: 478 378 6354
Fragments arranged by size:
6354 478 378
EcoRV GAT'ATC
Cuts at: 3010 3010
Size: 7210
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2317 2606 2828 4330 2317
Size: 289 222 1502 5197
Fragments arranged by size:
5197 1502 289 222
FspI TGC'GCA
Cuts at: 2836 5716 2836
Size: 2880 4330
HaeII r_GCGC'y
Cuts at: 1678 3179 6357 1678
Size: 1501 3178 2531
Fragments arranged by size:
3178 2531 1501
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2453 2485 2930 2962 3517
Size: 32 135 32 881 32 445 32 555
Cuts at: 3517 3549 3653 3685 4451 4469 4483 4501 5851
Size: 32 104 32 766 18 14 18 1350
Cuts at: 5851 5883 5925 5957 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1350 881 766 555 445 135 104
42 32 32 32 32 32 32 32
32 18 18 14
HincII GTy'rAC
Cuts at: 669 1657 1938 3436 4554 4800 669
Size: 988 281 1498 1118 246 3079
Fragments arranged by size:
3079 1498 1118 988 281 246
HindIII A'AGCT_T
Cuts at: 748 2282 4297 748
Size: 1534 2015 3661
Fragments arranged by size:
3661 2015 1534
HpaI GTT'AAC
Cuts at: 4800 4800
Size: 7210
KpnI G_GTAC'C
Cuts at: 4535 4535
Size: 7210
MfeI C'AATT_G
Cuts at: 4809 4809
Size: 7210
MluI A'CGCG_T
Cuts at: 7086 7086
Size: 7210
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2321 3453 3637 3684 4140 6324 6508 1305
Size: 1016 1132 184 47 456 2184 184 2007
Fragments arranged by size:
2184 2007 1132 1016 456 184 184 47
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2291 10
Size: 54 1425 343 459 4929
Fragments arranged by size:
4929 1425 459 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 4109 4427 4496 5168 5527 5686 518
Size: 648 2943 318 69 672 359 159 2042
Fragments arranged by size:
2943 2042 672 648 359 318 159 69
MspA1I CmG'CkG
Cuts at: 1606 2767 3151 3412 3780 3867 4626 5303 6451
Size: 1161 384 261 368 87 759 677 1148
Cuts at: 6451 6696 1606
Size: 245 2120
Fragments arranged by size:
2120 1161 1148 759 677 384 368 261
245 87
NaeI GCC'GGC
Cuts at: 4397 4397
Size: 7210
NcoI C'CATG_G
Cuts at: 513 1065 4239 513
Size: 552 3174 3484
Fragments arranged by size:
3484 3174 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7210
NgoMIV G'CCGG_C
Cuts at: 4395 4395
Size: 7210
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7210
NotI GC'GGCC_GC
Cuts at: 4657 4657
Size: 7210
NsiI A_TGCA'T
Cuts at: 2215 2215
Size: 7210
NspI r_CATG'y
Cuts at: 1132 1229 2755 2888 4568 6113 1132
Size: 97 1526 133 1680 1545 2229
Fragments arranged by size:
2229 1680 1545 1526 133 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 7210
PciI A'CATG_T
Cuts at: 6109 6109
Size: 7210
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 7210
PfoI T'CCnGG_A
Cuts at: 3051 3051
Size: 7210
PmeI GTTT'AAAC
Cuts at: 4514 4514
Size: 7210
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2233 2265 3791 3823 5279 5311 6792 6824 2233
Size: 32 1526 32 1456 32 1481 32 2619
Fragments arranged by size:
2619 1526 1481 1456 32 32 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7210
PsiI TTA'TAA
Cuts at: 4780 4780
Size: 7210
PspOMI G'GGCC_C
Cuts at: 2914 2914
Size: 7210
PstI C_TGCA'G
Cuts at: 830 3132 3525 4360 4562 830
Size: 2302 393 835 202 3478
Fragments arranged by size:
3478 2302 835 393 202
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7210
PvuII CAG'CTG
Cuts at: 3151 3412 3151
Size: 261 6949
SacI G_AGCT'C
Cuts at: 2656 2656
Size: 7210
SacII CC_GC'GG
Cuts at: 2768 2768
Size: 7210
SalI G'TCGA_C
Cuts at: 1936 4552 1936
Size: 2616 4594
SapI GCTCTTCn'nnn_
Cuts at: 2577 4406 2577
Size: 1829 5381
SbfI CC_TGCA'GG
Cuts at: 3132 4562 3132
Size: 1430 5780
ScaI AGT'ACT
Cuts at: 5458 7170 5458
Size: 1712 5498
SfcI C'TryA_G
Cuts at: 826 1045 3128 3514 3521 4356 4558 5693 6374
Size: 219 2083 386 7 835 202 1135 681
Cuts at: 6374 6565 826
Size: 191 1471
Fragments arranged by size:
2083 1471 1135 835 681 386 219 202
191 7
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7210
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7210
SmaI CCC'GGG
Cuts at: 1872 3902 4537 1872
Size: 2030 635 4545
Fragments arranged by size:
4545 2030 635
SmlI C'TyrA_G
Cuts at: 820 1017 1943 5314 6215 6477 820
Size: 197 926 3371 901 262 1553
Fragments arranged by size:
3371 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 5001 493
Size: 4508 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7210
SphI G_CATG'C
Cuts at: 1132 2888 4568 1132
Size: 1756 1680 3774
Fragments arranged by size:
3774 1756 1680
SspI AAT'ATT
Cuts at: 5 52 3397 5134 5
Size: 47 3345 1737 2081
Fragments arranged by size:
3345 2081 1737 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 7210
StyI C'CwwG_G
Cuts at: 513 1065 4239 4332 4470 4649 513
Size: 552 3174 93 138 179 3074
Fragments arranged by size:
3174 3074 552 179 138 93
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2189 5237 5254 5407 5592 6037 325
Size: 1255 609 3048 17 153 185 445 1498
Fragments arranged by size:
3048 1498 1255 609 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2817 3264 4122
Size: 274 80 33 51 1443 840 447 858
Cuts at: 4122 5456 7168 96
Size: 1334 1712 138
Fragments arranged by size:
1712 1443 1334 858 840 447 274 138
80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2482 2514 3955 3987 7170 7202 2482
Size: 32 1441 32 3183 32 2490
Fragments arranged by size:
3183 2490 1441 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 7210
XbaI T'CTAG_A
Cuts at: 4546 4546
Size: 7210
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7210
XmaI C'CCGG_G
Cuts at: 1870 3900 4535 1870
Size: 2030 635 4545
Fragments arranged by size:
4545 2030 635
XmnI GAAnn'nnTTC
Cuts at: 2245 4310 4313 5339 2245
Size: 2065 3 1026 4116
Fragments arranged by size:
4116 2065 1026 3
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3433 4480 5018 276
Size: 53 83 186 936 1899 1047 538 2468
Fragments arranged by size:
2468 1899 1047 936 538 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI
BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI
DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI
NsiI NspI PasI PciI PflMI PfoI PmeI PpiI
PshAI PsiI PspOMI PstI PvuI PvuII SacI SacII
SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AscI AvrII BaeI BbeI BsiWI BstXI BstZ17I
Bsu36I EcoNI FseI FspAI KasI NarI NruI PacI
PmlI PpuMI PspXI PsrI RsrII SanDI SexAI SfiI
SfoI SrfI SwaI XcmI