Restriction map for
pFN21AA0073
(Circular) (Six-base) MAPSORT of: pFN21AA0073.seq Check: 5654 from: 1 to: 6643
pFN21AA0073 6643 bp
With 182 enzymes: SgfI *
January 16, 2009 15:22 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4453 278
Size: 53 83 186 936 2917 2468
Fragments arranged by size:
2917 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3986 1937
Size: 2049 4594
Acc65I G'GTAC_C
Cuts at: 3964 3964
Size: 6643
AclI AA'CG_TT
Cuts at: 1563 4770 5143 1563
Size: 3207 373 3063
Fragments arranged by size:
3207 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2562 3275 3477 4706 6090 6358 1632
Size: 930 713 202 1229 1384 268 1917
Fragments arranged by size:
1917 1384 1229 930 713 268 202
AfeI AGC'GCT
Cuts at: 2150 2150
Size: 6643
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6446
AflIII A'CryG_T
Cuts at: 1203 2338 5542 6519 1203
Size: 1135 3204 977 1327
Fragments arranged by size:
3204 1327 1135 977
AgeI A'CCGG_T
Cuts at: 3162 5474 3162
Size: 2312 4331
AhdI GACnn_n'nnGTC
Cuts at: 5372 5372
Size: 6643
AleI CACnn'nnGTG
Cuts at: 1166 2288 1166
Size: 1122 5521
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2282 3264 1184
Size: 1098 982 4563
Fragments arranged by size:
4563 1098 982
AlwNI CAG_nnn'CTG
Cuts at: 2585 3395 3653 4386 5958 2585
Size: 810 258 733 1572 3270
Fragments arranged by size:
3270 1572 810 733 258
ApaLI G'TGCA_C
Cuts at: 4700 5856 4700
Size: 1156 5487
ApoI r'AATT_y
Cuts at: 1493 2375 2610 2777 2828 2919 3020 3686 3698
Size: 882 235 167 51 91 101 666 12
Cuts at: 3698 3952 4178 4330 6439 1493
Size: 254 226 152 2109 1697
Fragments arranged by size:
2109 1697 882 666 254 235 226 167
152 101 91 51 12
AseI AT'TA_AT
Cuts at: 160 1032 3077 3081 5197 160
Size: 872 2045 4 2116 1606
Fragments arranged by size:
2116 2045 1606 872 4
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6643
AvaI C'yCGr_G
Cuts at: 1870 1943 3968 1870
Size: 73 2025 4545
Fragments arranged by size:
4545 2025 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 3777 3810 3777
Size: 33 6610
BamHI G'GATC_C
Cuts at: 3770 3973 4340 3770
Size: 203 367 6073
Fragments arranged by size:
6073 367 203
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2049 3964 5419 618
Size: 325 217 627 262 1915 1455 1842
Fragments arranged by size:
1915 1842 1455 627 325 262 217
BanII G_rGCy'C
Cuts at: 1391 3962 1391
Size: 2571 4072
BbeI G_GCGC'C
Cuts at: 2053 2053
Size: 6643
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2715 928
Size: 971 816 4856
Fragments arranged by size:
4856 971 816
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4834 4868 1903
Size: 34 2897 34 3678
Fragments arranged by size:
3678 2897 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4535 5745 4535
Size: 1210 5433
BclI T'GATC_A
Cuts at: 1548 2505 1548
Size: 957 5686
BfrBI ATG'CAT
Cuts at: 2269 2269
Size: 6643
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2009 5254 136
Size: 107 122 71 631 605 337 3245 1525
Fragments arranged by size:
3245 1525 631 605 337 122 107 71
BglII A'GATC_T
Cuts at: 6638 6638
Size: 6643
BlpI GC'TnA_GC
Cuts at: 4060 4060
Size: 6643
Bme1580I G_kGCm'C
Cuts at: 1790 3542 4704 5860 1790
Size: 1752 1162 1156 2573
Fragments arranged by size:
2573 1752 1162 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6643
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2232 2696 2766
Size: 416 53 83 399 24 815 464 70
Cuts at: 2766 2806 3769 4374 5332 442
Size: 40 963 605 958 1753
Fragments arranged by size:
1753 963 958 815 605 464 416 399
83 70 53 40 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6643
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3657 5303 1239
Size: 2418 1646 2579
Fragments arranged by size:
2579 2418 1646
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3435 4768 5633 5931 3435
Size: 1333 865 298 4147
Fragments arranged by size:
4147 1333 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5306 882
Size: 617 3807 2219
Fragments arranged by size:
3807 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 4434 493
Size: 711 3230 2702
Fragments arranged by size:
3230 2702 711
BsaBI GATnn'nnATC
Cuts at: 3396 6637 3396
Size: 3241 3402
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2050 4450 4832 5467
Size: 53 83 186 936 517 2400 382 635
Cuts at: 5467 275
Size: 1451
Fragments arranged by size:
2400 1451 936 635 517 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2068 3162 5076 5474 5748 5895 6306 2068
Size: 1094 1914 398 274 147 411 2405
Fragments arranged by size:
2405 1914 1094 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2746 2776 1178
Size: 30 165 30 1343 30 5045
Fragments arranged by size:
5045 1343 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2150 2646 2754 3678 3696 1186
Size: 964 496 108 924 18 4133
Fragments arranged by size:
4133 964 924 496 108 18
BseYI C'CCAG_C
Cuts at: 2151 3130 5846 6342 2151
Size: 979 2716 496 2452
Fragments arranged by size:
2716 2452 979 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3408 1163
Size: 573 1672 4398
Fragments arranged by size:
4398 1672 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4093 4854 5882 1540
Size: 275 186 2092 761 1028 2301
Fragments arranged by size:
2301 2092 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 4704 4789 5860 4704
Size: 85 1071 5487
Fragments arranged by size:
5487 1071 85
BsmI GAATG_Cn'
Cuts at: 4154 4247 4154
Size: 93 6550
BsmBI CGTCTCn'nnnn_
Cuts at: 2032 2032
Size: 6643
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 3542 3962 4704 4789 5860 1391
Size: 399 1752 420 742 85 1071 2174
Fragments arranged by size:
2174 1752 1071 742 420 399 85
BspEI T'CCGG_A
Cuts at: 6306 6306
Size: 6643
BspHI T'CATG_A
Cuts at: 2329 2767 2946 4532 6262 2329
Size: 438 179 1586 1730 2710
Fragments arranged by size:
2710 1730 1586 438 179
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3998 844
Size: 3154 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 4530 1394
Size: 429 2707 3507
Fragments arranged by size:
3507 2707 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2478 5138 5312 65
Size: 1183 1230 2660 174 1396
Fragments arranged by size:
2660 1396 1230 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3162 5287 5474 1700
Size: 110 1352 2125 187 2869
Fragments arranged by size:
2869 2125 1352 187 110
BsrGI T'GTAC_A
Cuts at: 96 3323 3859 96
Size: 3227 536 2880
Fragments arranged by size:
3227 2880 536
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6643
BssSI C'ACGA_G
Cuts at: 4703 5715 4703
Size: 1012 5631
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6643
BstBI TT'CG_AA
Cuts at: 5447 5447
Size: 6643
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6643
BstXI CCAn_nnnn'nTGG
Cuts at: 3804 3804
Size: 6643
BstYI r'GATC_y
Cuts at: 1972 2550 3640 3770 3867 3973 4340 4725 4742
Size: 578 1090 130 97 106 367 385 17
Cuts at: 4742 6194 6280 6292 6638 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 1090 578 385 367 346 130
106 97 86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 5152
Fragments arranged by size:
5152 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5904
Fragments arranged by size:
5904 683 56
BtsI GCAGTG_nn'
Cuts at: 2115 4159 4953 4973 2115
Size: 2044 794 20 3785
Fragments arranged by size:
3785 2044 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6643
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6608
DraI TTT'AAA
Cuts at: 3947 3947
Size: 6643
DraIII CAC_nnn'GTG
Cuts at: 3112 3112
Size: 6643
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5650 809
Size: 507 4334 1802
Fragments arranged by size:
4334 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2184 2626 3025
Size: 54 1425 50 275 18 354 442 399
Cuts at: 3025 4090 4979 6608 8
Size: 1065 889 1629 43
Fragments arranged by size:
1629 1425 1065 889 442 399 354 275
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4090 1537
Size: 275 2278 4090
Fragments arranged by size:
4090 2278 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2139 2186 2358 4573 1353
Size: 786 47 172 2215 3423
Fragments arranged by size:
3423 2215 786 172 47
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2027 5230 5604 5750 387
Size: 1064 138 438 3203 374 146 1280
Fragments arranged by size:
3203 1280 1064 438 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2562 3275 3477 3657 4706 5303 6090
Size: 393 930 713 202 180 1049 597 787
Cuts at: 6090 6358 1239
Size: 268 1524
Fragments arranged by size:
1524 1049 930 787 713 597 393 268
202 180
EcoO109I rG'GnC_Cy
Cuts at: 2056 3461 2056
Size: 1405 5238
EcoRI G'AATT_C
Cuts at: 3952 4330 3952
Size: 378 6265
FspI TGC'GCA
Cuts at: 5149 5149
Size: 6643
HaeII r_GCGC'y
Cuts at: 1678 2053 2152 5790 1678
Size: 375 99 3638 2531
Fragments arranged by size:
3638 2531 375 99
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2265 2297 2746 2778 3625
Size: 32 135 32 693 32 449 32 847
Cuts at: 3625 3657 5284 5316 5358 5390 1373
Size: 32 1627 32 42 32 2626
Fragments arranged by size:
2626 1627 847 693 449 135 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3987 4233 669
Size: 988 281 2049 246 3079
Fragments arranged by size:
3079 2049 988 281 246
HindIII A'AGCT_T
Cuts at: 748 3270 748
Size: 2522 4121
HpaI GTT'AAC
Cuts at: 4233 4233
Size: 6643
KasI G'GCGC_C
Cuts at: 2049 2049
Size: 6643
KpnI G_GTAC'C
Cuts at: 3968 3968
Size: 6643
MfeI C'AATT_G
Cuts at: 3209 4242 3209
Size: 1033 5610
MluI A'CGCG_T
Cuts at: 6519 6519
Size: 6643
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2137 2876 3033 3668 3897 5757 5941 1305
Size: 832 739 157 635 229 1860 184 2007
Fragments arranged by size:
2007 1860 832 739 635 229 184 157
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2186 10
Size: 54 1425 343 354 4467
Fragments arranged by size:
4467 1425 354 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2274 2288 3500 4601 4960 5119 518
Size: 648 1108 14 1212 1101 359 159 2042
Fragments arranged by size:
2042 1212 1108 1101 648 359 159 14
MspA1I CmG'CkG
Cuts at: 1606 4059 4736 5884 6129 1606
Size: 2453 677 1148 245 2120
Fragments arranged by size:
2453 2120 1148 677 245
NarI GG'CG_CC
Cuts at: 2050 2050
Size: 6643
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 5152
Fragments arranged by size:
5152 939 552
NdeI CA'TA_TG
Cuts at: 387 3674 387
Size: 3287 3356
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6643
NotI GC'GGCC_GC
Cuts at: 4090 4090
Size: 6643
NsiI A_TGCA'T
Cuts at: 2271 2271
Size: 6643
NspI r_CATG'y
Cuts at: 1132 1229 2269 2342 2515 2977 4001 5546 1132
Size: 97 1040 73 173 462 1024 1545 2229
Fragments arranged by size:
2229 1545 1040 1024 462 173 97 73
PacI TTA_AT'TAA
Cuts at: 3081 3081
Size: 6643
PasI CC'CwG_GG
Cuts at: 1348 2561 1348
Size: 1213 5430
PciI A'CATG_T
Cuts at: 2338 5542 2338
Size: 3204 3439
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6643
PfoI T'CCnGG_A
Cuts at: 2533 2533
Size: 6643
PmeI GTTT'AAAC
Cuts at: 3947 3947
Size: 6643
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4712 4744 6225 6257 4712
Size: 32 1481 32 5098
Fragments arranged by size:
5098 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2056 3461 2056
Size: 1405 5238
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6643
PsiI TTA'TAA
Cuts at: 3146 3588 4213 3146
Size: 442 625 5576
Fragments arranged by size:
5576 625 442
PstI C_TGCA'G
Cuts at: 830 3995 830
Size: 3165 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6643
SalI G'TCGA_C
Cuts at: 1936 3985 1936
Size: 2049 4594
SanDI GG'GwC_CC
Cuts at: 2056 2056
Size: 6643
SapI GCTCTTCn'nnn_
Cuts at: 2139 2139
Size: 6643
SbfI CC_TGCA'GG
Cuts at: 3995 3995
Size: 6643
ScaI AGT'ACT
Cuts at: 4891 6603 4891
Size: 1712 4931
SfcI C'TryA_G
Cuts at: 826 1045 3039 3258 3991 5126 5807 5998 826
Size: 219 1994 219 733 1135 681 191 1471
Fragments arranged by size:
1994 1471 1135 733 681 219 219 191
SfoI GGC'GCC
Cuts at: 2051 2051
Size: 6643
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6643
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6643
SmaI CCC'GGG
Cuts at: 1872 3970 1872
Size: 2098 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3450 4747 5648 5910 820
Size: 197 926 1507 1297 901 262 1553
Fragments arranged by size:
1553 1507 1297 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4434 493
Size: 3941 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6643
SphI G_CATG'C
Cuts at: 1132 4001 1132
Size: 2869 3774
SspI AAT'ATT
Cuts at: 5 52 3196 3298 3931 4567 5
Size: 47 3144 102 633 636 2081
Fragments arranged by size:
3144 2081 636 633 102 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6643
StyI C'CwwG_G
Cuts at: 513 1065 2004 3803 4082 513
Size: 552 939 1799 279 3074
Fragments arranged by size:
3074 1799 939 552 279
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2300 2447 4670 4687 4840 5025 5470
Size: 1255 720 147 2223 17 153 185 445
Cuts at: 5470 325
Size: 1498
Fragments arranged by size:
2223 1498 1255 720 445 185 153 147
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2600 3323 3859
Size: 274 80 33 51 1443 623 723 536
Cuts at: 3859 4889 6601 96
Size: 1030 1712 138
Fragments arranged by size:
1712 1443 1030 723 623 536 274 138
80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3096 3128 6603 6635 3096
Size: 32 3475 32 3104
Fragments arranged by size:
3475 3104 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6643
XbaI T'CTAG_A
Cuts at: 3979 3979
Size: 6643
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6643
XmaI C'CCGG_G
Cuts at: 1870 3968 1870
Size: 2098 4545
XmnI GAAnn'nnTTC
Cuts at: 4772 4772
Size: 6643
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4451 276
Size: 53 83 186 936 2917 2468
Fragments arranged by size:
2917 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BaeI BamHI BanI BanII BbeI BbsI
BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII
DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI
FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI
MfeI MluI MmeI MscI MslI MspA1I NarI NcoI
NdeI NheI NotI NsiI NspI PacI PasI PciI
PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI
PvuI SalI SanDI SapI SbfI ScaI SfcI SfoI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AarI AloI ApaI AscI AvrII BbvCI BplI Bpu10I
BsiWI BstZ17I Bsu36I EcoICRI EcoNI EcoRV FalI FseI
FspAI NaeI NgoMIV NruI PmlI PspOMI PspXI PsrI
PvuII RsrII SacI SacII SexAI SfiI SrfI SwaI
XcmI