Restriction map for
pFN21AA0072
(Circular) (Six-base) MAPSORT of: pFN21AA0072.seq Check: 9990 from: 1 to: 6217
pFN21AA0072 6217 bp
With 182 enzymes: SgfI *
April 15, 2010 09:25 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4027 278
Size: 53 83 186 936 2491 2468
Fragments arranged by size:
2491 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2952 3560 1937
Size: 1015 608 4594
Fragments arranged by size:
4594 1015 608
Acc65I G'GTAC_C
Cuts at: 3538 3538
Size: 6217
AclI AA'CG_TT
Cuts at: 1563 4344 4717 1563
Size: 2781 373 3063
Fragments arranged by size:
3063 2781 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 4280 5664 5932 1632
Size: 2648 1384 268 1917
Fragments arranged by size:
2648 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6020
AflIII A'CryG_T
Cuts at: 1203 5116 6093 1203
Size: 3913 977 1327
Fragments arranged by size:
3913 1327 977
AgeI A'CCGG_T
Cuts at: 2233 3333 5048 2233
Size: 1100 1715 3402
Fragments arranged by size:
3402 1715 1100
AhdI GACnn_n'nnGTC
Cuts at: 4946 4946
Size: 6217
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6217
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6217
AlwNI CAG_nnn'CTG
Cuts at: 2641 3960 5532 2641
Size: 1319 1572 3326
Fragments arranged by size:
3326 1572 1319
ApaLI G'TGCA_C
Cuts at: 4274 5430 4274
Size: 1156 5061
ApoI r'AATT_y
Cuts at: 1493 2341 2760 3526 3752 3904 6013 1493
Size: 848 419 766 226 152 2109 1697
Fragments arranged by size:
2109 1697 848 766 419 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4771 160
Size: 872 3739 1606
Fragments arranged by size:
3739 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6217
AvaI C'yCGr_G
Cuts at: 1870 1943 3180 3542 1870
Size: 73 1237 362 4545
Fragments arranged by size:
4545 1237 362 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2596 2629 2596
Size: 33 6184
BamHI G'GATC_C
Cuts at: 2972 3184 3547 3914 2972
Size: 212 363 367 5275
Fragments arranged by size:
5275 367 363 212
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3538 4993 618
Size: 325 217 627 1751 1455 1842
Fragments arranged by size:
1842 1751 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 3198 3286 3536 1391
Size: 1807 88 250 4072
Fragments arranged by size:
4072 1807 250 88
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5246
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4408 4442 1903
Size: 34 2471 34 3678
Fragments arranged by size:
3678 2471 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2209 4109 5319 2209
Size: 1900 1210 3107
Fragments arranged by size:
3107 1900 1210
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6217
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4828 136
Size: 107 122 71 631 605 3156 1525
Fragments arranged by size:
3156 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2868 6212 2868
Size: 3344 2873
BlpI GC'TnA_GC
Cuts at: 3634 3634
Size: 6217
Bme1580I G_kGCm'C
Cuts at: 1790 4278 5434 1790
Size: 2488 1156 2573
Fragments arranged by size:
2573 2488 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6217
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3059 3948 4906
Size: 416 53 83 399 24 1642 889 958
Cuts at: 4906 442
Size: 1753
Fragments arranged by size:
1753 1642 958 889 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6217
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2081 2228 2501 3212 4877 1239
Size: 842 147 273 711 1665 2579
Fragments arranged by size:
2579 1665 842 711 273 147
Bpu10I CC'TnA_GC
Cuts at: 3123 3123
Size: 6217
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2724 4342 5207 5505 2724
Size: 1618 865 298 3436
Fragments arranged by size:
3436 1618 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4880 882
Size: 617 3381 2219
Fragments arranged by size:
3381 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2242 4008 493
Size: 711 1038 1766 2702
Fragments arranged by size:
2702 1766 1038 711
BsaBI GATnn'nnATC
Cuts at: 6211 6211
Size: 6217
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4024 4406 5041 275
Size: 53 83 186 936 2491 382 635 1451
Fragments arranged by size:
2491 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2233 3333 4650 5048 5322 5469 5880 2233
Size: 1100 1317 398 274 147 411 2570
Fragments arranged by size:
2570 1317 1100 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3501 3531 1178
Size: 30 165 30 2098 30 3864
Fragments arranged by size:
3864 2098 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2078 1186
Size: 892 5325
BseYI C'CCAG_C
Cuts at: 2634 5420 5916 2634
Size: 2786 496 2935
Fragments arranged by size:
2935 2786 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5644
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3667 4428 5456 1540
Size: 275 186 1666 761 1028 2301
Fragments arranged by size:
2301 1666 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3198 4278 4363 5434 3198
Size: 1080 85 1071 3981
Fragments arranged by size:
3981 1080 1071 85
BsmI GAATG_Cn'
Cuts at: 3064 3728 3821 3064
Size: 664 93 5460
Fragments arranged by size:
5460 664 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 3198 3286 3536 4278 4363 5434 1391
Size: 399 1408 88 250 742 85 1071 2174
Fragments arranged by size:
2174 1408 1071 742 399 250 88 85
BspEI T'CCGG_A
Cuts at: 5880 5880
Size: 6217
BspHI T'CATG_A
Cuts at: 4106 5836 4106
Size: 1730 4487
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2274 3572 844
Size: 1430 1298 3489
Fragments arranged by size:
3489 1430 1298
BsrBI CCG'CTC
Cuts at: 1394 1823 2104 2149 4104 1394
Size: 429 281 45 1955 3507
Fragments arranged by size:
3507 1955 429 281 45
BsrDI GCAATG_nn'
Cuts at: 65 1248 3264 4712 4886 65
Size: 1183 2016 1448 174 1396
Fragments arranged by size:
2016 1448 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2233 3333 4861 5048 1700
Size: 110 423 1100 1528 187 2869
Fragments arranged by size:
2869 1528 1100 423 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6217
BssHII G'CGCG_C
Cuts at: 1925 2073 1925
Size: 148 6069
BssSI C'ACGA_G
Cuts at: 4277 5289 4277
Size: 1012 5205
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2098 794
Size: 1304 4913
BstBI TT'CG_AA
Cuts at: 5021 5021
Size: 6217
BstEII G'GTnAC_C
Cuts at: 1184 2629 1184
Size: 1445 4772
BstYI r'GATC_y
Cuts at: 1972 2868 2972 3184 3547 3914 4299 4316 5768
Size: 896 104 212 363 367 385 17 1452
Cuts at: 5768 5854 5866 6212 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 896 385 367 363 346 212
104 86 17 12
Bsu36I CC'TnA_GG
Cuts at: 2255 2255
Size: 6217
BtgI C'CryG_G
Cuts at: 513 1065 2004 3202 513
Size: 552 939 1198 3528
Fragments arranged by size:
3528 1198 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5478
Fragments arranged by size:
5478 683 56
BtsI GCAGTG_nn'
Cuts at: 2103 3733 4527 4547 2103
Size: 1630 794 20 3773
Fragments arranged by size:
3773 1630 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6217
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2920 2955 529
Size: 35 2356 35 3791
Fragments arranged by size:
3791 2356 35 35
DraI TTT'AAA
Cuts at: 2604 3521 2604
Size: 917 5300
DraIII CAC_nnn'GTG
Cuts at: 2086 2086
Size: 6217
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5224 809
Size: 507 3908 1802
Fragments arranged by size:
3908 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3261 3664 4553
Size: 54 1425 50 275 18 1431 403 889
Cuts at: 4553 6182 8
Size: 1629 43
Fragments arranged by size:
1629 1431 1425 889 403 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3664 1537
Size: 275 1852 4090
Fragments arranged by size:
4090 1852 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2156 3111 3203 4147 1353
Size: 803 955 92 944 3423
Fragments arranged by size:
3423 955 944 803 92
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2144 4804 5178 5324 387
Size: 1064 138 555 2660 374 146 1280
Fragments arranged by size:
2660 1280 1064 555 374 146 138
EcoICRI GAG'CTC
Cuts at: 3196 3196
Size: 6217
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2081 2228 2501 3212 4280 4877 5664
Size: 393 449 147 273 711 1068 597 787
Cuts at: 5664 5932 1239
Size: 268 1524
Fragments arranged by size:
1524 1068 787 711 597 449 393 273
268 147
EcoO109I rG'GnC_Cy
Cuts at: 2926 2926
Size: 6217
EcoRI G'AATT_C
Cuts at: 3526 3904 3526
Size: 378 5839
FspI TGC'GCA
Cuts at: 4723 4723
Size: 6217
HaeII r_GCGC'y
Cuts at: 1678 2027 2057 5364 1678
Size: 349 30 3307 2531
Fragments arranged by size:
3307 2531 349 30
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2405 2437 2783 2815 2905
Size: 32 135 32 833 32 346 32 90
Cuts at: 2905 2937 4858 4890 4932 4964 1373
Size: 32 1921 32 42 32 2626
Fragments arranged by size:
2626 1921 833 346 135 90 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2263 3561 3807 669
Size: 988 281 325 1298 246 3079
Fragments arranged by size:
3079 1298 988 325 281 246
HindIII A'AGCT_T
Cuts at: 748 3354 748
Size: 2606 3611
HpaI GTT'AAC
Cuts at: 3807 3807
Size: 6217
KpnI G_GTAC'C
Cuts at: 3542 3542
Size: 6217
MfeI C'AATT_G
Cuts at: 3408 3816 3408
Size: 408 5809
MluI A'CGCG_T
Cuts at: 6093 6093
Size: 6217
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2941 5331 5515 1305
Size: 1636 2390 184 2007
Fragments arranged by size:
2390 2007 1636 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4395
Fragments arranged by size:
4395 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2289 4175 4534 4693 518
Size: 648 1123 1886 359 159 2042
Fragments arranged by size:
2042 1886 1123 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2059 3633 4310 5458 5703 1606
Size: 453 1574 677 1148 245 2120
Fragments arranged by size:
2120 1574 1148 677 453 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4726
Fragments arranged by size:
4726 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6217
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6217
NotI GC'GGCC_GC
Cuts at: 3664 3664
Size: 6217
NspI r_CATG'y
Cuts at: 1132 1229 3575 5120 1132
Size: 97 2346 1545 2229
Fragments arranged by size:
2346 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 3279 1348
Size: 1931 4286
PciI A'CATG_T
Cuts at: 5116 5116
Size: 6217
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2020 2227 1108
Size: 912 207 5098
Fragments arranged by size:
5098 912 207
PmeI GTTT'AAAC
Cuts at: 3521 3521
Size: 6217
PmlI CAC'GTG
Cuts at: 2242 2242
Size: 6217
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4286 4318 5799 5831 4286
Size: 32 1481 32 4672
Fragments arranged by size:
4672 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2926 2926
Size: 6217
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6217
PsiI TTA'TAA
Cuts at: 3787 3787
Size: 6217
PstI C_TGCA'G
Cuts at: 830 2094 2271 3569 830
Size: 1264 177 1298 3478
Fragments arranged by size:
3478 1298 1264 177
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6217
SacI G_AGCT'C
Cuts at: 3198 3198
Size: 6217
SalI G'TCGA_C
Cuts at: 1936 3559 1936
Size: 1623 4594
SapI GCTCTTCn'nnn_
Cuts at: 2156 3203 2156
Size: 1047 5170
SbfI CC_TGCA'GG
Cuts at: 3569 3569
Size: 6217
ScaI AGT'ACT
Cuts at: 4465 6177 4465
Size: 1712 4505
SfcI C'TryA_G
Cuts at: 826 1045 2090 2267 2424 2949 3565 4700 5381
Size: 219 1045 177 157 525 616 1135 681
Cuts at: 5381 5572 826
Size: 191 1471
Fragments arranged by size:
1471 1135 1045 681 616 525 219 191
177 157
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6217
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6217
SmaI CCC'GGG
Cuts at: 1872 3544 1872
Size: 1672 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2739 4321 5222 5484 820
Size: 197 926 796 1582 901 262 1553
Fragments arranged by size:
1582 1553 926 901 796 262 197
SnaBI TAC'GTA
Cuts at: 493 4008 493
Size: 3515 2702
SpeI A'CTAG_T
Cuts at: 152 3379 152
Size: 3227 2990
SphI G_CATG'C
Cuts at: 1132 3575 1132
Size: 2443 3774
SspI AAT'ATT
Cuts at: 5 52 4141 5
Size: 47 4089 2081
Fragments arranged by size:
4089 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6217
StyI C'CwwG_G
Cuts at: 513 1065 2004 3656 513
Size: 552 939 1652 3074
Fragments arranged by size:
3074 1652 939 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4244 4261 4414 4599 5044 325
Size: 1255 2664 17 153 185 445 1498
Fragments arranged by size:
2664 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3111 4463 6175
Size: 274 80 33 51 1443 1134 1352 1712
Cuts at: 6175 96
Size: 138
Fragments arranged by size:
1712 1443 1352 1134 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6177 6209 6177
Size: 32 6185
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6217
XbaI T'CTAG_A
Cuts at: 3553 3553
Size: 6217
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2623 2623
Size: 6217
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6217
XmaI C'CCGG_G
Cuts at: 1870 3542 1870
Size: 1672 4545
XmnI GAAnn'nnTTC
Cuts at: 2235 4346 2235
Size: 2111 4106
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4025 276
Size: 53 83 186 936 2491 2468
Fragments arranged by size:
2491 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BaeI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI
BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NcoI NdeI NheI NotI NspI
PasI PciI PflMI PmeI PmlI PpiI PpuMI PshAI
PsiI PstI PvuI SacI SalI SapI SbfI ScaI
SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BbeI BbvCI
BfrBI BplI BsiWI BsmBI BstXI BstZ17I EcoNI EcoRV
FalI FseI FspAI KasI NaeI NarI NgoMIV NruI
NsiI PacI PfoI PspOMI PspXI PsrI PvuII RsrII
SacII SanDI SexAI SfiI SfoI SrfI SwaI