Restriction map for
pFN21AA0068
(Circular) (Six-base) MAPSORT of: pFN21AA0068.seq Check: 4949 from: 1 to: 8464
pFN21AA0068 8464 bp
With 182 enzymes: SgfI *
January 16, 2009 15:22 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3379 3379
Size: 8464
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 6274 278
Size: 53 83 186 936 4738 2468
Fragments arranged by size:
4738 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 5415 5807 1937
Size: 3478 392 4594
Fragments arranged by size:
4594 3478 392
Acc65I G'GTAC_C
Cuts at: 4446 5785 4446
Size: 1339 7125
AclI AA'CG_TT
Cuts at: 1563 6591 6964 1563
Size: 5028 373 3063
Fragments arranged by size:
5028 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2546 3321 4274 4826 4964 4991 5702 6527
Size: 914 775 953 552 138 27 711 825
Cuts at: 6527 7911 8179 1632
Size: 1384 268 1917
Fragments arranged by size:
1917 1384 953 914 825 775 711 552
268 138 27
AfeI AGC'GCT
Cuts at: 3298 3298
Size: 8464
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 8267
AflIII A'CryG_T
Cuts at: 1203 2502 4497 7363 8340 1203
Size: 1299 1995 2866 977 1327
Fragments arranged by size:
2866 1995 1327 1299 977
AgeI A'CCGG_T
Cuts at: 4590 7295 4590
Size: 2705 5759
AhdI GACnn_n'nnGTC
Cuts at: 2035 2461 7193 2035
Size: 426 4732 3306
Fragments arranged by size:
4732 3306 426
AleI CACnn'nnGTG
Cuts at: 1166 5708 1166
Size: 4542 3922
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 4665 4856 1184
Size: 3481 191 4792
Fragments arranged by size:
4792 3481 191
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3401 3433 3401
Size: 32 8432
AlwNI CAG_nnn'CTG
Cuts at: 2632 2674 3196 3394 4063 4666 4993 5044 6207
Size: 42 522 198 669 603 327 51 1163
Cuts at: 6207 7779 2632
Size: 1572 3317
Fragments arranged by size:
3317 1572 1163 669 603 522 327 198
51 42
ApaI G_GGCC'C
Cuts at: 3765 3765
Size: 8464
ApaLI G'TGCA_C
Cuts at: 3004 3254 6521 7677 3004
Size: 250 3267 1156 3791
Fragments arranged by size:
3791 3267 1156 250
ApoI r'AATT_y
Cuts at: 1493 2125 2374 2492 3785 3823 4625 5625 5773
Size: 632 249 118 1293 38 802 1000 148
Cuts at: 5773 5999 6151 8260 1493
Size: 226 152 2109 1697
Fragments arranged by size:
2109 1697 1293 1000 802 632 249 226
152 148 118 38
AseI AT'TA_AT
Cuts at: 160 1032 7018 160
Size: 872 5986 1606
Fragments arranged by size:
5986 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8464
AvaI C'yCGr_G
Cuts at: 1870 1943 2274 3127 5789 1870
Size: 73 331 853 2662 4545
Fragments arranged by size:
4545 2662 853 331 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 4762 4795 4762
Size: 33 8431
BamHI G'GATC_C
Cuts at: 3909 3942 4673 5301 5794 6161 3909
Size: 33 731 628 493 367 6212
Fragments arranged by size:
6212 731 628 493 367 33
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2427 4446 5785 7240 618
Size: 325 217 627 640 2019 1339 1455 1842
Fragments arranged by size:
2019 1842 1455 1339 640 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2097 2715 3128 3585 3765 4545 5783 1391
Size: 706 618 413 457 180 780 1238 4072
Fragments arranged by size:
4072 1238 780 706 618 457 413 180
BbeI G_GCGC'C
Cuts at: 2431 2431
Size: 8464
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2151 3155 3557 4525 4859 4979 928
Size: 971 252 1004 402 968 334 120 4413
Fragments arranged by size:
4413 1004 971 968 402 334 252 120
BbvCI CC'TCA_GC
Cuts at: 5211 5211
Size: 8464
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2985 3019 3157 3191 4176 4210 6655
Size: 34 1048 34 138 34 985 34 2445
Cuts at: 6655 6689 1903
Size: 34 3678
Fragments arranged by size:
3678 2445 1048 985 138 34 34 34
34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4928 6356 7566 4928
Size: 1428 1210 5826
Fragments arranged by size:
5826 1428 1210
BclI T'GATC_A
Cuts at: 1548 2478 3375 4305 5493 1548
Size: 930 897 930 1188 4519
Fragments arranged by size:
4519 1188 930 930 897
BfrBI ATG'CAT
Cuts at: 2156 5362 2156
Size: 3206 5258
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2009 3493 7075
Size: 107 122 71 631 605 337 1484 3582
Cuts at: 7075 136
Size: 1525
Fragments arranged by size:
3582 1525 1484 631 605 337 122 107
71
BglII A'GATC_T
Cuts at: 4878 8459 4878
Size: 3581 4883
BlpI GC'TnA_GC
Cuts at: 4439 5881 4439
Size: 1442 7022
Bme1580I G_kGCm'C
Cuts at: 1790 3008 3258 3765 5705 6525 7681 1790
Size: 1218 250 507 1940 820 1156 2573
Fragments arranged by size:
2573 1940 1218 1156 820 507 250
BmgBI CAC'GTC
Cuts at: 1318 3003 4531 1318
Size: 1685 1528 5251
Fragments arranged by size:
5251 1685 1528
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2495 2769 3574
Size: 416 53 83 399 24 1078 274 805
Cuts at: 3574 5210 5488 6195 7153 442
Size: 1636 278 707 958 1753
Fragments arranged by size:
1753 1636 1078 958 805 707 416 399
278 274 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 4124 1056
Size: 3068 5396
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2595 4924 2595
Size: 2329 6135
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2045 2075 2243 2281 2369 2894 3652 3908
Size: 806 30 168 38 88 525 758 256
Cuts at: 3908 3983 4684 4943 7124 1239
Size: 75 701 259 2181 2579
Fragments arranged by size:
2579 2181 806 758 701 525 259 256
168 88 75 38 30
Bpu10I CC'TnA_GC
Cuts at: 5211 5211
Size: 8464
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3763 3776 6589 7454 7752 3763
Size: 13 2813 865 298 4475
Fragments arranged by size:
4475 2813 865 298 13
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3959 7127 882
Size: 617 2460 3168 2219
Fragments arranged by size:
3168 2460 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2591 3226 3994 4849 6255 493
Size: 711 1387 635 768 855 1406 2702
Fragments arranged by size:
2702 1406 1387 855 768 711 635
BsaBI GATnn'nnATC
Cuts at: 3056 8458 3056
Size: 5402 3062
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2428 2771 4470 6271
Size: 53 83 186 936 895 343 1699 1801
Cuts at: 6271 6653 7288 275
Size: 382 635 1451
Fragments arranged by size:
1801 1699 1451 936 895 635 382 343
186 83 53
BsaWI w'CCGG_w
Cuts at: 4590 4719 5304 6897 7295 7569 7716 8127 4590
Size: 129 585 1593 398 274 147 411 4927
Fragments arranged by size:
4927 1593 585 411 398 274 147 129
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2824 2854 3257 3287 3401
Size: 30 165 30 1421 30 403 30 114
Cuts at: 3401 3431 1178
Size: 30 6211
Fragments arranged by size:
6211 1421 403 165 114 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2072 2240 2278 2461 3356 4265 4811 1186
Size: 886 168 38 183 895 909 546 4839
Fragments arranged by size:
4839 909 895 886 546 183 168 38
BseYI C'CCAG_C
Cuts at: 3595 4311 5345 7667 8163 3595
Size: 716 1034 2322 496 3896
Fragments arranged by size:
3896 2322 1034 716 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2560 4289 4511 5057 1163
Size: 573 824 1729 222 546 4570
Fragments arranged by size:
4570 1729 824 573 546 222
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 5914 6675 7703 1540
Size: 275 186 3913 761 1028 2301
Fragments arranged by size:
3913 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2715 3008 3258 3999 4545 6525 6610 7681 2715
Size: 293 250 741 546 1980 85 1071 3498
Fragments arranged by size:
3498 1980 1071 741 546 293 250 85
BsmI GAATG_Cn'
Cuts at: 4199 5421 5975 6068 4199
Size: 1222 554 93 6595
Fragments arranged by size:
6595 1222 554 93
BsmBI CGTCTCn'nnnn_
Cuts at: 3563 4238 4324 3563
Size: 675 86 7703
Fragments arranged by size:
7703 675 86
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2097 2715 3008 3128 3258 3585 3765
Size: 399 307 618 293 120 130 327 180
Cuts at: 3765 3999 4545 5705 5783 6525 6610 7681 1391
Size: 234 546 1160 78 742 85 1071 2174
Fragments arranged by size:
2174 1160 1071 742 618 546 399 327
307 293 234 180 130 120 85 78
BspEI T'CCGG_A
Cuts at: 5304 8127 5304
Size: 2823 5641
BspHI T'CATG_A
Cuts at: 6353 8083 6353
Size: 1730 6734
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3379 5001 5076 5250 5819 844
Size: 2535 1622 75 174 569 3489
Fragments arranged by size:
3489 2535 1622 569 174 75
BsrBI CCG'CTC
Cuts at: 1394 1823 2579 3627 6351 1394
Size: 429 756 1048 2724 3507
Fragments arranged by size:
3507 2724 1048 756 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3711 5356 6959 7133 65
Size: 1183 2463 1645 1603 174 1396
Fragments arranged by size:
2463 1645 1603 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4443 4590 4758 7108 7295 1700
Size: 110 2633 147 168 2350 187 2869
Fragments arranged by size:
2869 2633 2350 187 168 147 110
BsrGI T'GTAC_A
Cuts at: 96 2253 4014 96
Size: 2157 1761 4546
Fragments arranged by size:
4546 2157 1761
BssHII G'CGCG_C
Cuts at: 1925 3095 3219 1925
Size: 1170 124 7170
Fragments arranged by size:
7170 1170 124
BssSI C'ACGA_G
Cuts at: 4898 5438 6524 7536 4898
Size: 540 1086 1012 5826
Fragments arranged by size:
5826 1086 1012 540
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3196 794
Size: 2402 6062
BstBI TT'CG_AA
Cuts at: 7268 7268
Size: 8464
BstEII G'GTnAC_C
Cuts at: 1184 3472 1184
Size: 2288 6176
BstXI CCAn_nnnn'nTGG
Cuts at: 2644 3772 2644
Size: 1128 7336
BstYI r'GATC_y
Cuts at: 1972 2602 3057 3909 3942 4673 4878 5301 5794
Size: 630 455 852 33 731 205 423 493
Cuts at: 5794 6161 6546 6563 8015 8101 8113 8459 1972
Size: 367 385 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 852 731 630 493 455 423
385 367 346 205 86 33 17 12
Bsu36I CC'TnA_GG
Cuts at: 2355 3479 2355
Size: 1124 7340
BtgI C'CryG_G
Cuts at: 513 1065 2004 2337 3657 3897 4785 5427 513
Size: 552 939 333 1320 240 888 642 3550
Fragments arranged by size:
3550 1320 939 888 642 552 333 240
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2155 5209 487
Size: 683 56 929 3054 3742
Fragments arranged by size:
3742 3054 929 683 56
BtsI GCAGTG_nn'
Cuts at: 2548 3218 3452 3851 5462 5980 6774 6794 2548
Size: 670 234 399 1611 518 794 20 4218
Fragments arranged by size:
4218 1611 794 670 518 399 234 20
ClaI AT'CG_AT
Cuts at: 1554 3980 1554
Size: 2426 6038
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 8429
DraI TTT'AAA
Cuts at: 5768 5768
Size: 8464
DraIII CAC_nnn'GTG
Cuts at: 4498 4498
Size: 8464
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 7471 809
Size: 507 6155 1802
Fragments arranged by size:
6155 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2646 5911 6800
Size: 54 1425 50 275 18 816 3265 889
Cuts at: 6800 8429 8
Size: 1629 43
Fragments arranged by size:
3265 1629 1425 889 816 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 5911 1537
Size: 275 4099 4090
Fragments arranged by size:
4099 4090 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2703 3179 3297 5024 6394 1353
Size: 1350 476 118 1727 1370 3423
Fragments arranged by size:
3423 1727 1370 1350 476 118
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 7051 7425 7571 387
Size: 1064 138 5462 374 146 1280
Fragments arranged by size:
5462 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2713 4543 2713
Size: 1830 6634
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2045 2075 2243 2281 2369 2546 2894
Size: 393 413 30 168 38 88 177 348
Cuts at: 2894 3321 3652 3908 3983 4274 4684 4826 4943
Size: 427 331 256 75 291 410 142 117
Cuts at: 4943 4964 4991 5702 6527 7124 7911 8179 1239
Size: 21 27 711 825 597 787 268 1524
Fragments arranged by size:
1524 825 787 711 597 427 413 410
393 348 331 291 268 256 177 168
142 117 88 75 38 30 27 21
EcoNI CCTnn'n_nnAGG
Cuts at: 2054 3359 4513 4997 2054
Size: 1305 1154 484 5521
Fragments arranged by size:
5521 1305 1154 484
EcoO109I rG'GnC_Cy
Cuts at: 3193 3616 3662 3761 4733 5205 5239 3193
Size: 423 46 99 972 472 34 6418
Fragments arranged by size:
6418 972 472 423 99 46 34
EcoRI G'AATT_C
Cuts at: 5625 5773 6151 5625
Size: 148 378 7938
Fragments arranged by size:
7938 378 148
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3646 3757 3646
Size: 111 8353
FspI TGC'GCA
Cuts at: 6970 6970
Size: 8464
HaeII r_GCGC'y
Cuts at: 1678 2431 2973 3188 3300 4029 4508 5260 7611
Size: 753 542 215 112 729 479 752 2351
Cuts at: 7611 1678
Size: 2531
Fragments arranged by size:
2531 2351 753 752 729 542 479 215
112
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2021 2053 2307 2339 2447
Size: 32 135 32 449 32 254 32 108
Cuts at: 2447 2479 2594 2626 3257 3289 4200 4232 4367
Size: 32 115 32 631 32 911 32 135
Cuts at: 4367 4399 7105 7137 7179 7211 1373
Size: 32 2706 32 42 32 2626
Fragments arranged by size:
2706 2626 911 631 449 254 135 135
115 108 42 32 32 32 32 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 5808 6054 669
Size: 988 281 3870 246 3079
Fragments arranged by size:
3870 3079 988 281 246
HindIII A'AGCT_T
Cuts at: 748 3248 748
Size: 2500 5964
HpaI GTT'AAC
Cuts at: 6054 6054
Size: 8464
KasI G'GCGC_C
Cuts at: 2427 2427
Size: 8464
KpnI G_GTAC'C
Cuts at: 4450 5789 4450
Size: 1339 7125
MfeI C'AATT_G
Cuts at: 6063 6063
Size: 8464
MluI A'CGCG_T
Cuts at: 8340 8340
Size: 8464
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2064 2835 3300 4389 7578 7762 1305
Size: 759 771 465 1089 3189 184 2007
Fragments arranged by size:
3189 2007 1089 771 759 465 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2648 10
Size: 54 1425 343 816 5826
Fragments arranged by size:
5826 1425 816 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2501 3926 5363 5426 5708 6422 6781
Size: 648 1335 1425 1437 63 282 714 359
Cuts at: 6781 6940 518
Size: 159 2042
Fragments arranged by size:
2042 1437 1425 1335 714 648 359 282
159 63
MspA1I CmG'CkG
Cuts at: 1606 3202 3490 3865 4942 5173 5518 5880 6557
Size: 1596 288 375 1077 231 345 362 677
Cuts at: 6557 7705 7950 1606
Size: 1148 245 2120
Fragments arranged by size:
2120 1596 1148 1077 677 375 362 345
288 245 231
NaeI GCC'GGC
Cuts at: 4760 4760
Size: 8464
NarI GG'CG_CC
Cuts at: 2428 2428
Size: 8464
NcoI C'CATG_G
Cuts at: 513 1065 2004 3897 513
Size: 552 939 1893 5080
Fragments arranged by size:
5080 1893 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 8464
NgoMIV G'CCGG_C
Cuts at: 4758 4758
Size: 8464
NheI G'CTAG_C
Cuts at: 1052 4120 1052
Size: 3068 5396
NotI GC'GGCC_GC
Cuts at: 5911 5911
Size: 8464
NsiI A_TGCA'T
Cuts at: 2158 5364 2158
Size: 3206 5258
NspI r_CATG'y
Cuts at: 1132 1229 2506 2791 2938 3076 4268 5366 5720
Size: 97 1277 285 147 138 1192 1098 354
Cuts at: 5720 5822 7367 1132
Size: 102 1545 2229
Fragments arranged by size:
2229 1545 1277 1192 1098 354 285 147
138 102 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 8464
PciI A'CATG_T
Cuts at: 2502 7363 2502
Size: 4861 3603
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3961 1108
Size: 2853 5611
PfoI T'CCnGG_A
Cuts at: 2052 2616 3242 3885 3960 4479 4920 5667 2052
Size: 564 626 643 75 519 441 747 4849
Fragments arranged by size:
4849 747 643 626 564 519 441 75
PmeI GTTT'AAAC
Cuts at: 5768 5768
Size: 8464
PmlI CAC'GTG
Cuts at: 3226 3226
Size: 8464
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2543 2575 3401 3433 6533 6565 8046 8078 2543
Size: 32 826 32 3100 32 1481 32 2929
Fragments arranged by size:
3100 2929 1481 826 32 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 3616 3662 4733 5205 5239 3616
Size: 46 1071 472 34 6841
Fragments arranged by size:
6841 1071 472 46 34
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 8464
PsiI TTA'TAA
Cuts at: 4146 6034 4146
Size: 1888 6576
PspOMI G'GGCC_C
Cuts at: 3761 3761
Size: 8464
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 4679 4711 4679
Size: 32 8432
PstI C_TGCA'G
Cuts at: 830 2679 3192 3201 3390 3459 3540 3843 4845
Size: 1849 513 9 189 69 81 303 1002
Cuts at: 4845 5816 830
Size: 971 3478
Fragments arranged by size:
3478 1849 1002 971 513 303 189 81
69 9
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 8464
PvuII CAG'CTG
Cuts at: 3202 3865 4942 3202
Size: 663 1077 6724
Fragments arranged by size:
6724 1077 663
SacI G_AGCT'C
Cuts at: 2715 4545 2715
Size: 1830 6634
SalI G'TCGA_C
Cuts at: 1936 5806 1936
Size: 3870 4594
SanDI GG'GwC_CC
Cuts at: 3662 5205 3662
Size: 1543 6921
SapI GCTCTTCn'nnn_
Cuts at: 2703 3179 2703
Size: 476 7988
SbfI CC_TGCA'GG
Cuts at: 3390 3540 5816 3390
Size: 150 2276 6038
Fragments arranged by size:
6038 2276 150
ScaI AGT'ACT
Cuts at: 2897 3272 6712 8424 2897
Size: 375 3440 1712 2937
Fragments arranged by size:
3440 2937 1712 375
SexAI A'CCwGG_T
Cuts at: 4043 4043
Size: 8464
SfcI C'TryA_G
Cuts at: 826 1045 2675 3100 3188 3197 3386 3455 3536
Size: 219 1630 425 88 9 189 69 81
Cuts at: 3536 3839 4841 5812 6947 7628 7819 826
Size: 303 1002 971 1135 681 191 1471
Fragments arranged by size:
1630 1471 1135 1002 971 681 425 303
219 191 189 88 81 69 9
SfoI GGC'GCC
Cuts at: 2429 2429
Size: 8464
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8464
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 8464
SmaI CCC'GGG
Cuts at: 1872 2276 5791 1872
Size: 404 3515 4545
Fragments arranged by size:
4545 3515 404
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3742 3755 6568 7469 7731 820
Size: 197 926 1799 13 2813 901 262 1553
Fragments arranged by size:
2813 1799 1553 926 901 262 197 13
SnaBI TAC'GTA
Cuts at: 493 2591 6255 493
Size: 2098 3664 2702
Fragments arranged by size:
3664 2702 2098
SpeI A'CTAG_T
Cuts at: 152 152
Size: 8464
SphI G_CATG'C
Cuts at: 1132 5822 1132
Size: 4690 3774
SspI AAT'ATT
Cuts at: 5 52 6388 5
Size: 47 6336 2081
Fragments arranged by size:
6336 2081 47
StuI AGG'CCT
Cuts at: 1517 3384 1517
Size: 1867 6597
StyI C'CwwG_G
Cuts at: 513 1065 2004 3897 3969 4677 5903 513
Size: 552 939 1893 72 708 1226 3074
Fragments arranged by size:
3074 1893 1226 939 708 552 72
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4325 6491 6508 6661 6846 7291 325
Size: 1255 2745 2166 17 153 185 445 1498
Fragments arranged by size:
2745 2166 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2253 2382 2895
Size: 274 80 33 51 1443 276 129 513
Cuts at: 2895 3030 3270 4014 6710 8422 96
Size: 135 240 744 2696 1712 138
Fragments arranged by size:
2696 1712 1443 744 513 276 274 240
138 135 129 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2598 2630 8424 8456 2598
Size: 32 5794 32 2606
Fragments arranged by size:
5794 2606 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 8464
XbaI T'CTAG_A
Cuts at: 5800 5800
Size: 8464
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 4393 5570 4393
Size: 1177 7287
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 8464
XmaI C'CCGG_G
Cuts at: 1870 2274 5789 1870
Size: 404 3515 4545
Fragments arranged by size:
4545 3515 404
XmnI GAAnn'nnTTC
Cuts at: 2456 3786 6593 2456
Size: 1330 2807 4327
Fragments arranged by size:
4327 2807 1330
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 6272 276
Size: 53 83 186 936 4738 2468
Fragments arranged by size:
4738 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI
ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI
BanII BbeI BbsI BbvCI BcgI BciVI BclI BfrBI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I
BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI
KasI KpnI MfeI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NdeI NgoMIV NheI NotI NsiI
NspI PasI PciI PflMI PfoI PmeI PmlI PpiI
PpuMI PshAI PsiI PspOMI PsrI PstI PvuI PvuII
SacI SalI SanDI SapI SbfI ScaI SexAI SfcI
SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AscI AvrII BsiWI BstZ17I EcoRV FseI FspAI NruI
PacI PspXI RsrII SacII SfiI SrfI SwaI