Restriction map for
pFN21AA0060
(Circular) (Six-base) MAPSORT of: pFN21AA0060.seq Check: 5128 from: 1 to: 5572
pFN21AA0060 5572 bp
With 182 enzymes: SgfI *
February 27, 2009 14:31 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2378 2378
Size: 5572
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3382 278
Size: 53 83 186 936 1846 2468
Fragments arranged by size:
2468 1846 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2340 2915 1937
Size: 403 575 4594
Fragments arranged by size:
4594 575 403
Acc65I G'GTAC_C
Cuts at: 2893 2893
Size: 5572
AclI AA'CG_TT
Cuts at: 1563 3699 4072 1563
Size: 2136 373 3063
Fragments arranged by size:
3063 2136 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3635 5019 5287 1632
Size: 2003 1384 268 1917
Fragments arranged by size:
2003 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5375
AflIII A'CryG_T
Cuts at: 1203 2670 4471 5448 1203
Size: 1467 1801 977 1327
Fragments arranged by size:
1801 1467 1327 977
AgeI A'CCGG_T
Cuts at: 4403 4403
Size: 5572
AhdI GACnn_n'nnGTC
Cuts at: 4301 4301
Size: 5572
AleI CACnn'nnGTG
Cuts at: 1166 2543 1166
Size: 1377 4195
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5572
AlwNI CAG_nnn'CTG
Cuts at: 3315 4887 3315
Size: 1572 4000
ApaLI G'TGCA_C
Cuts at: 3629 4785 3629
Size: 1156 4416
ApoI r'AATT_y
Cuts at: 1493 2881 3107 3259 5368 1493
Size: 1388 226 152 2109 1697
Fragments arranged by size:
2109 1697 1388 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4126 160
Size: 872 3094 1606
Fragments arranged by size:
3094 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5572
AvaI C'yCGr_G
Cuts at: 1870 1943 2238 2897 1870
Size: 73 295 659 4545
Fragments arranged by size:
4545 659 295 73
BamHI G'GATC_C
Cuts at: 2902 3269 2902
Size: 367 5205
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2545 2893 4348 618
Size: 325 217 627 758 348 1455 1842
Fragments arranged by size:
1842 1455 758 627 348 325 217
BanII G_rGCy'C
Cuts at: 1391 2121 2891 1391
Size: 730 770 4072
Fragments arranged by size:
4072 770 730
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2210 2489 2759 928
Size: 971 311 279 270 3741
Fragments arranged by size:
3741 971 311 279 270
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3763 3797 1903
Size: 34 1826 34 3678
Fragments arranged by size:
3678 1826 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2491 3464 4674 2491
Size: 973 1210 3389
Fragments arranged by size:
3389 1210 973
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5572
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4183 136
Size: 107 122 71 631 605 2511 1525
Fragments arranged by size:
2511 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5567 5567
Size: 5572
BlpI GC'TnA_GC
Cuts at: 2989 2989
Size: 5572
Bme1580I G_kGCm'C
Cuts at: 1790 2550 2579 3633 4789 1790
Size: 760 29 1054 1156 2573
Fragments arranged by size:
2573 1156 1054 760 29
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5572
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2136 3303 4261
Size: 416 53 83 399 24 719 1167 958
Cuts at: 4261 442
Size: 1753
Fragments arranged by size:
1753 1167 958 719 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5572
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2470 2470
Size: 5572
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2042 2440 4232 1239
Size: 803 398 1792 2579
Fragments arranged by size:
2579 1792 803 398
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3697 4562 4860 3697
Size: 865 298 4409
Fragments arranged by size:
4409 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2235 4235 882
Size: 617 736 2000 2219
Fragments arranged by size:
2219 2000 736 617
BsaAI yAC'GTr
Cuts at: 493 1204 2227 3363 493
Size: 711 1023 1136 2702
Fragments arranged by size:
2702 1136 1023 711
BsaBI GATnn'nnATC
Cuts at: 2609 5566 2609
Size: 2957 2615
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3379 3761 4396 275
Size: 53 83 186 936 1846 382 635 1451
Fragments arranged by size:
1846 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4005 4403 4677 4824 5235 4005
Size: 398 274 147 411 4342
Fragments arranged by size:
4342 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5347
Fragments arranged by size:
5347 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2669 1186
Size: 1483 4089
BseYI C'CCAG_C
Cuts at: 4775 5271 4775
Size: 496 5076
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 4999
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3022 3783 4811 1540
Size: 275 186 1021 761 1028 2301
Fragments arranged by size:
2301 1028 1021 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2028 2623 3633 3718 4789 2028
Size: 595 1010 85 1071 2811
Fragments arranged by size:
2811 1071 1010 595 85
BsmI GAATG_Cn'
Cuts at: 2338 3083 3176 2338
Size: 745 93 4734
Fragments arranged by size:
4734 745 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2028 2121 2550 2579 2623 2891 3633
Size: 399 238 93 429 29 44 268 742
Cuts at: 3633 3718 4789 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 742 429 399 268 238 93
85 44 29
BspEI T'CCGG_A
Cuts at: 5235 5235
Size: 5572
BspHI T'CATG_A
Cuts at: 3461 5191 3461
Size: 1730 3842
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2378 2927 844
Size: 1534 549 3489
Fragments arranged by size:
3489 1534 549
BsrBI CCG'CTC
Cuts at: 1394 1823 3459 1394
Size: 429 1636 3507
Fragments arranged by size:
3507 1636 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2433 4067 4241 65
Size: 1183 1185 1634 174 1396
Fragments arranged by size:
1634 1396 1185 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4216 4403 1700
Size: 110 2406 187 2869
Fragments arranged by size:
2869 2406 187 110
BsrGI T'GTAC_A
Cuts at: 96 2640 2811 96
Size: 2544 171 2857
Fragments arranged by size:
2857 2544 171
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5572
BssSI C'ACGA_G
Cuts at: 3632 4644 3632
Size: 1012 4560
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5572
BstBI TT'CG_AA
Cuts at: 2852 4376 2852
Size: 1524 4048
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5572
BstYI r'GATC_y
Cuts at: 1972 2902 3269 3654 3671 5123 5209 5221 5567
Size: 930 367 385 17 1452 86 12 346
Cuts at: 5567 1972
Size: 1977
Fragments arranged by size:
1977 1452 930 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2493 2553 513
Size: 552 1428 60 3532
Fragments arranged by size:
3532 1428 552 60
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2883 487
Size: 683 56 1657 3176
Fragments arranged by size:
3176 1657 683 56
BtsI GCAGTG_nn'
Cuts at: 3088 3882 3902 3088
Size: 794 20 4758
Fragments arranged by size:
4758 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5572
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5537
DraI TTT'AAA
Cuts at: 2194 2876 2194
Size: 682 4890
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4579 809
Size: 507 3263 1802
Fragments arranged by size:
3263 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2490 2508 3019
Size: 54 1425 50 275 18 660 18 511
Cuts at: 3019 3908 5537 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 889 660 511 275 54 50
43 18 18
EagI C'GGCC_G
Cuts at: 1537 1812 3019 1537
Size: 275 1207 4090
Fragments arranged by size:
4090 1207 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2364 3502 1353
Size: 1011 1138 3423
Fragments arranged by size:
3423 1138 1011
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4159 4533 4679 387
Size: 1064 138 2570 374 146 1280
Fragments arranged by size:
2570 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2042 2440 3635 4232 5019 5287 1239
Size: 393 410 398 1195 597 787 268 1524
Fragments arranged by size:
1524 1195 787 597 410 398 393 268
EcoO109I rG'GnC_Cy
Cuts at: 2182 2472 2182
Size: 290 5282
EcoRI G'AATT_C
Cuts at: 2881 3259 2881
Size: 378 5194
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2865 2865
Size: 5572
FspI TGC'GCA
Cuts at: 4078 4078
Size: 5572
HaeII r_GCGC'y
Cuts at: 1678 4719 1678
Size: 3041 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4213 4245 4287 4319 1373
Size: 32 135 32 2641 32 42 32 2626
Fragments arranged by size:
2641 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2341 2916 3162 669
Size: 988 281 403 575 246 3079
Fragments arranged by size:
3079 988 575 403 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5572
HpaI GTT'AAC
Cuts at: 3162 3162
Size: 5572
KpnI G_GTAC'C
Cuts at: 2897 2897
Size: 5572
MfeI C'AATT_G
Cuts at: 3171 3171
Size: 5572
MluI A'CGCG_T
Cuts at: 5448 5448
Size: 5572
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2389 4686 4870 1305
Size: 1084 2297 184 2007
Fragments arranged by size:
2297 2007 1084 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2492 2510 10
Size: 54 1425 343 660 18 3072
Fragments arranged by size:
3072 1425 660 343 54 18
MslI CAynn'nnrTG
Cuts at: 518 1166 2543 2582 3530 3889 4048 518
Size: 648 1377 39 948 359 159 2042
Fragments arranged by size:
2042 1377 948 648 359 159 39
MspA1I CmG'CkG
Cuts at: 1606 2988 3665 4813 5058 1606
Size: 1382 677 1148 245 2120
Fragments arranged by size:
2120 1382 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2493 2553 513
Size: 552 1428 60 3532
Fragments arranged by size:
3532 1428 552 60
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5572
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5572
NotI GC'GGCC_GC
Cuts at: 3019 3019
Size: 5572
NspI r_CATG'y
Cuts at: 1132 1229 2674 2930 4475 1132
Size: 97 1445 256 1545 2229
Fragments arranged by size:
2229 1545 1445 256 97
PasI CC'CwG_GG
Cuts at: 1348 2090 2113 1348
Size: 742 23 4807
Fragments arranged by size:
4807 742 23
PciI A'CATG_T
Cuts at: 2670 4471 2670
Size: 1801 3771
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2323 1108
Size: 1215 4357
PfoI T'CCnGG_A
Cuts at: 2019 2468 2019
Size: 449 5123
PmeI GTTT'AAAC
Cuts at: 2876 2876
Size: 5572
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3641 3673 5154 5186 3641
Size: 32 1481 32 4027
Fragments arranged by size:
4027 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2182 2472 2182
Size: 290 5282
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5572
PsiI TTA'TAA
Cuts at: 3142 3142
Size: 5572
PstI C_TGCA'G
Cuts at: 830 2389 2924 830
Size: 1559 535 3478
Fragments arranged by size:
3478 1559 535
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5572
SalI G'TCGA_C
Cuts at: 1936 2339 2914 1936
Size: 403 575 4594
Fragments arranged by size:
4594 575 403
SbfI CC_TGCA'GG
Cuts at: 2924 2924
Size: 5572
ScaI AGT'ACT
Cuts at: 2105 3820 5532 2105
Size: 1715 1712 2145
Fragments arranged by size:
2145 1715 1712
SfcI C'TryA_G
Cuts at: 826 1045 2345 2385 2920 4055 4736 4927 826
Size: 219 1300 40 535 1135 681 191 1471
Fragments arranged by size:
1471 1300 1135 681 535 219 191 40
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5572
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5572
SmaI CCC'GGG
Cuts at: 1872 2899 1872
Size: 1027 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2238 3676 4577 4839 820
Size: 197 926 295 1438 901 262 1553
Fragments arranged by size:
1553 1438 926 901 295 262 197
SnaBI TAC'GTA
Cuts at: 493 3363 493
Size: 2870 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5572
SphI G_CATG'C
Cuts at: 1132 2930 1132
Size: 1798 3774
SspI AAT'ATT
Cuts at: 5 52 3496 5
Size: 47 3444 2081
Fragments arranged by size:
3444 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5572
StyI C'CwwG_G
Cuts at: 513 1065 2493 2541 2553 2736 3011 513
Size: 552 1428 48 12 183 275 3074
Fragments arranged by size:
3074 1428 552 275 183 48 12
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2319 2330 3599 3616 3769 3954 4399
Size: 1255 739 11 1269 17 153 185 445
Cuts at: 4399 325
Size: 1498
Fragments arranged by size:
1498 1269 1255 739 445 185 153 17
11
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2103 2640 2811
Size: 274 80 33 51 1443 126 537 171
Cuts at: 2811 3818 5530 96
Size: 1007 1712 138
Fragments arranged by size:
1712 1443 1007 537 274 171 138 126
80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5532 5564 5532
Size: 32 5540
Tth111I GACn'n_nGTC
Cuts at: 1316 2680 1316
Size: 1364 4208
XbaI T'CTAG_A
Cuts at: 2908 2908
Size: 5572
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2324 2324
Size: 5572
XhoI C'TCGA_G
Cuts at: 1943 2238 1943
Size: 295 5277
XmaI C'CCGG_G
Cuts at: 1870 2897 1870
Size: 1027 4545
XmnI GAAnn'nnTTC
Cuts at: 2105 2207 2279 3701 2105
Size: 102 72 1422 3976
Fragments arranged by size:
3976 1422 102 72
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3380 276
Size: 53 83 186 936 1846 2468
Fragments arranged by size:
2468 1846 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NcoI NdeI NheI NotI NspI PasI PciI
PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI
PvuI SalI SbfI ScaI SfcI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI
BfrBI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII
EcoICRI EcoNI EcoRV FseI FspAI KasI NaeI NarI
NgoMIV NruI NsiI PacI PmlI PspOMI PspXI PsrI
PvuII RsrII SacI SacII SanDI SapI SexAI SfiI
SfoI SrfI SwaI