Restriction map for
pFN21AA0059
(Circular) (Six-base) MAPSORT of: pFN21AA0059.seq Check: 3540 from: 1 to: 6859
pFN21AA0059 6859 bp
With 182 enzymes: SgfI *
February 27, 2009 14:36 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2204 2711 2204
Size: 507 6352
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3622 4669 278
Size: 53 83 186 936 2086 1047 2468
Fragments arranged by size:
2468 2086 1047 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2952 4202 1937
Size: 1015 1250 4594
Fragments arranged by size:
4594 1250 1015
Acc65I G'GTAC_C
Cuts at: 3138 4180 3138
Size: 1042 5817
AclI AA'CG_TT
Cuts at: 1563 4986 5359 1563
Size: 3423 373 3063
Fragments arranged by size:
3423 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 4083 4922 6306 6574 1632
Size: 2451 839 1384 268 1917
Fragments arranged by size:
2451 1917 1384 839 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6662
AflIII A'CryG_T
Cuts at: 1203 3951 5758 6735 1203
Size: 2748 1807 977 1327
Fragments arranged by size:
2748 1807 1327 977
AgeI A'CCGG_T
Cuts at: 5690 5690
Size: 6859
AhdI GACnn_n'nnGTC
Cuts at: 5588 5588
Size: 6859
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6859
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6859
AlwNI CAG_nnn'CTG
Cuts at: 2539 4602 6174 2539
Size: 2063 1572 3224
Fragments arranged by size:
3224 2063 1572
ApaLI G'TGCA_C
Cuts at: 2451 2748 4916 6072 2451
Size: 297 2168 1156 3238
Fragments arranged by size:
3238 2168 1156 297
ApoI r'AATT_y
Cuts at: 1493 4168 4394 4546 6655 1493
Size: 2675 226 152 2109 1697
Fragments arranged by size:
2675 2109 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 3644 5413 160
Size: 872 2612 1769 1606
Fragments arranged by size:
2612 1769 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6859
AvaI C'yCGr_G
Cuts at: 1870 1943 4184 1870
Size: 73 2241 4545
Fragments arranged by size:
4545 2241 73
BamHI G'GATC_C
Cuts at: 4189 4556 4189
Size: 367 6492
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3138 3487 4180 5635 618
Size: 325 217 627 1351 349 693 1455 1842
Fragments arranged by size:
1842 1455 1351 693 627 349 325 217
BanII G_rGCy'C
Cuts at: 1391 2034 3188 4178 1391
Size: 643 1154 990 4072
Fragments arranged by size:
4072 1154 990 643
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2852 928
Size: 971 953 4935
Fragments arranged by size:
4935 971 953
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5050 5084 1903
Size: 34 3113 34 3678
Fragments arranged by size:
3678 3113 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4751 5961 4751
Size: 1210 5649
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6859
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5470 136
Size: 107 122 71 631 605 3798 1525
Fragments arranged by size:
3798 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 3028 3578 6854 3028
Size: 550 3276 3033
Fragments arranged by size:
3276 3033 550
BlpI GC'TnA_GC
Cuts at: 4276 4276
Size: 6859
Bme1580I G_kGCm'C
Cuts at: 1790 2455 2752 4920 6076 1790
Size: 665 297 2168 1156 2573
Fragments arranged by size:
2573 2168 1156 665 297
BmgBI CAC'GTC
Cuts at: 1318 3234 1318
Size: 1916 4943
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4590 5548 442
Size: 416 53 83 399 24 3173 958 1753
Fragments arranged by size:
3173 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6859
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2273 2273
Size: 6859
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2476 2735 2893 4064 5519 1239
Size: 1237 259 158 1171 1455 2579
Fragments arranged by size:
2579 1455 1237 1171 259 158
Bpu10I CC'TnA_GC
Cuts at: 3342 3342
Size: 6859
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4984 5849 6147 4984
Size: 865 298 5696
Fragments arranged by size:
5696 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2400 5522 882
Size: 617 901 3122 2219
Fragments arranged by size:
3122 2219 901 617
BsaAI yAC'GTr
Cuts at: 493 1204 4650 493
Size: 711 3446 2702
Fragments arranged by size:
3446 2702 711
BsaBI GATnn'nnATC
Cuts at: 6853 6853
Size: 6859
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3619 4666 5048 5683
Size: 53 83 186 936 2086 1047 382 635
Cuts at: 5683 275
Size: 1451
Fragments arranged by size:
2086 1451 1047 936 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 5292 5690 5964 6111 6522 5292
Size: 398 274 147 411 5629
Fragments arranged by size:
5629 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2397 2427 3100 3130 1178
Size: 30 165 30 994 30 673 30 4907
Fragments arranged by size:
4907 994 673 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3948 1186
Size: 2762 4097
BseYI C'CCAG_C
Cuts at: 2741 3246 3475 3497 6062 6558 2741
Size: 505 229 22 2565 496 3042
Fragments arranged by size:
3042 2565 505 496 229 22
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2245 1163
Size: 573 509 5777
Fragments arranged by size:
5777 573 509
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3286 4309 5070 6098 1540
Size: 275 186 1285 1023 761 1028 2301
Fragments arranged by size:
2301 1285 1028 1023 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2034 2455 2635 2752 4920 5005 6076 2034
Size: 421 180 117 2168 85 1071 2817
Fragments arranged by size:
2817 2168 1071 421 180 117 85
BsmI GAATG_Cn'
Cuts at: 4370 4463 4370
Size: 93 6766
BsmBI CGTCTCn'nnnn_
Cuts at: 3512 3610 4117 3512
Size: 98 507 6254
Fragments arranged by size:
6254 507 98
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2034 2455 2635 2752 3188 4178 4920
Size: 399 244 421 180 117 436 990 742
Cuts at: 4920 5005 6076 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 990 742 436 421 399 244
180 117 85
BspEI T'CCGG_A
Cuts at: 6522 6522
Size: 6859
BspHI T'CATG_A
Cuts at: 4748 6478 4748
Size: 1730 5129
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2204 2711 4214 844
Size: 1360 507 1503 3489
Fragments arranged by size:
3489 1503 1360 507
BsrBI CCG'CTC
Cuts at: 1394 1823 3240 4746 1394
Size: 429 1417 1506 3507
Fragments arranged by size:
3507 1506 1417 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3274 3924 5354 5528 65
Size: 1183 2026 650 1430 174 1396
Fragments arranged by size:
2026 1430 1396 1183 650 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2504 3306 3819 5503 5690 1700
Size: 110 694 802 513 1684 187 2869
Fragments arranged by size:
2869 1684 802 694 513 187 110
BsrGI T'GTAC_A
Cuts at: 96 3204 96
Size: 3108 3751
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6859
BssSI C'ACGA_G
Cuts at: 3175 4919 5931 3175
Size: 1744 1012 4103
Fragments arranged by size:
4103 1744 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2539 2589 2763 794
Size: 1745 50 174 4890
Fragments arranged by size:
4890 1745 174 50
BstBI TT'CG_AA
Cuts at: 5663 5663
Size: 6859
BstEII G'GTnAC_C
Cuts at: 1184 3991 4110 1184
Size: 2807 119 3933
Fragments arranged by size:
3933 2807 119
BstXI CCAn_nnnn'nTGG
Cuts at: 2129 2306 3263 3533 3955 2129
Size: 177 957 270 422 5033
Fragments arranged by size:
5033 957 422 270 177
BstYI r'GATC_y
Cuts at: 1972 2961 3028 3578 4189 4556 4941 4958 6410
Size: 989 67 550 611 367 385 17 1452
Cuts at: 6410 6496 6508 6854 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 989 611 550 385 367 346
86 67 17 12
Bsu36I CC'TnA_GG
Cuts at: 2079 2079
Size: 6859
BtgI C'CryG_G
Cuts at: 513 1065 2052 2299 513
Size: 552 987 247 5073
Fragments arranged by size:
5073 987 552 247
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6120
Fragments arranged by size:
6120 683 56
BtsI GCAGTG_nn'
Cuts at: 2116 2564 4375 5169 5189 2116
Size: 448 1811 794 20 3786
Fragments arranged by size:
3786 1811 794 448 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6859
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2188 2223 529
Size: 35 1624 35 5165
Fragments arranged by size:
5165 1624 35 35
DraI TTT'AAA
Cuts at: 2578 4163 2578
Size: 1585 5274
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5866 809
Size: 507 4550 1802
Fragments arranged by size:
4550 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3283 3308 3904
Size: 54 1425 50 275 18 1453 25 596
Cuts at: 3904 4306 5195 6824 8
Size: 402 889 1629 43
Fragments arranged by size:
1629 1453 1425 889 596 402 275 54
50 43 25 18
EagI C'GGCC_G
Cuts at: 1537 1812 3283 4306 1537
Size: 275 1471 1023 4090
Fragments arranged by size:
4090 1471 1023 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2509 3630 3681 4160 4789 1353
Size: 1156 1121 51 479 629 3423
Fragments arranged by size:
3423 1156 1121 629 479 51
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5446 5820 5966 387
Size: 1064 138 3857 374 146 1280
Fragments arranged by size:
3857 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2032 2032
Size: 6859
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2476 2735 2893 4064 4083 4922 5519
Size: 393 844 259 158 1171 19 839 597
Cuts at: 5519 6306 6574 1239
Size: 787 268 1524
Fragments arranged by size:
1524 1171 844 839 787 597 393 268
259 158 19
EcoNI CCTnn'n_nnAGG
Cuts at: 4022 4022
Size: 6859
EcoO109I rG'GnC_Cy
Cuts at: 2083 2599 3346 3560 2083
Size: 516 747 214 5382
Fragments arranged by size:
5382 747 516 214
EcoRI G'AATT_C
Cuts at: 4168 4546 4168
Size: 378 6481
EcoRV GAT'ATC
Cuts at: 2521 3454 2521
Size: 933 5926
FspI TGC'GCA
Cuts at: 5365 5365
Size: 6859
HaeII r_GCGC'y
Cuts at: 1678 2392 2540 2549 4051 6006 1678
Size: 714 148 9 1502 1955 2531
Fragments arranged by size:
2531 1955 1502 714 148 9
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2341 2373 2397 2429 3100
Size: 32 135 32 769 32 24 32 671
Cuts at: 3100 3132 3151 3183 3660 3692 5500 5532 5574
Size: 32 19 32 477 32 1808 32 42
Cuts at: 5574 5606 1373
Size: 32 2626
Fragments arranged by size:
2626 1808 769 671 477 135 42 32
32 32 32 32 32 32 32 32
24 19
HincII GTy'rAC
Cuts at: 669 1657 1938 2444 4203 4449 669
Size: 988 281 506 1759 246 3079
Fragments arranged by size:
3079 1759 988 506 281 246
HindIII A'AGCT_T
Cuts at: 748 3650 748
Size: 2902 3957
HpaI GTT'AAC
Cuts at: 4449 4449
Size: 6859
KpnI G_GTAC'C
Cuts at: 3142 4184 3142
Size: 1042 5817
MfeI C'AATT_G
Cuts at: 2362 2497 4458 2362
Size: 135 1961 4763
Fragments arranged by size:
4763 1961 135
MluI A'CGCG_T
Cuts at: 6735 6735
Size: 6859
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3251 3356 3936 5973 6157 1305
Size: 1946 105 580 2037 184 2007
Fragments arranged by size:
2037 2007 1946 580 184 105
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3906 10
Size: 54 1425 343 2074 2963
Fragments arranged by size:
2963 2074 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2009 2127 2304 3360 4817 5176 5335
Size: 648 843 118 177 1056 1457 359 159
Cuts at: 5335 518
Size: 2042
Fragments arranged by size:
2042 1457 1056 843 648 359 177 159
118
MspA1I CmG'CkG
Cuts at: 1606 2278 2745 3282 3870 4275 4952 6100 6345
Size: 672 467 537 588 405 677 1148 245
Cuts at: 6345 1606
Size: 2120
Fragments arranged by size:
2120 1148 677 672 588 537 467 405
245
NaeI GCC'GGC
Cuts at: 2506 2506
Size: 6859
NcoI C'CATG_G
Cuts at: 513 1065 2052 2299 513
Size: 552 987 247 5073
Fragments arranged by size:
5073 987 552 247
NdeI CA'TA_TG
Cuts at: 387 3976 387
Size: 3589 3270
NgoMIV G'CCGG_C
Cuts at: 2504 2504
Size: 6859
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6859
NotI GC'GGCC_GC
Cuts at: 4306 4306
Size: 6859
NspI r_CATG'y
Cuts at: 1132 1229 3419 3913 3955 4217 5762 1132
Size: 97 2190 494 42 262 1545 2229
Fragments arranged by size:
2229 2190 1545 494 262 97 42
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6859
PciI A'CATG_T
Cuts at: 3951 5758 3951
Size: 1807 5052
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2817 1108
Size: 1709 5150
PfoI T'CCnGG_A
Cuts at: 2712 2964 2712
Size: 252 6607
PmeI GTTT'AAAC
Cuts at: 4163 4163
Size: 6859
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4928 4960 6441 6473 4928
Size: 32 1481 32 5314
Fragments arranged by size:
5314 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2083 2599 3560 2083
Size: 516 961 5382
Fragments arranged by size:
5382 961 516
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6859
PsiI TTA'TAA
Cuts at: 4429 4429
Size: 6859
PstI C_TGCA'G
Cuts at: 830 4211 830
Size: 3381 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6859
PvuII CAG'CTG
Cuts at: 2745 2745
Size: 6859
SacI G_AGCT'C
Cuts at: 2034 2034
Size: 6859
SalI G'TCGA_C
Cuts at: 1936 4201 1936
Size: 2265 4594
SapI GCTCTTCn'nnn_
Cuts at: 3630 3630
Size: 6859
SbfI CC_TGCA'GG
Cuts at: 4211 4211
Size: 6859
ScaI AGT'ACT
Cuts at: 5107 6819 5107
Size: 1712 5147
SexAI A'CCwGG_T
Cuts at: 2926 2926
Size: 6859
SfcI C'TryA_G
Cuts at: 826 1045 4207 5342 6023 6214 826
Size: 219 3162 1135 681 191 1471
Fragments arranged by size:
3162 1471 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6859
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6859
SmaI CCC'GGG
Cuts at: 1872 4186 1872
Size: 2314 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4963 5864 6126 820
Size: 197 926 3020 901 262 1553
Fragments arranged by size:
3020 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4650 493
Size: 4157 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6859
SphI G_CATG'C
Cuts at: 1132 3419 4217 1132
Size: 2287 798 3774
Fragments arranged by size:
3774 2287 798
SspI AAT'ATT
Cuts at: 5 52 4783 5
Size: 47 4731 2081
Fragments arranged by size:
4731 2081 47
StuI AGG'CCT
Cuts at: 1517 3663 1517
Size: 2146 4713
StyI C'CwwG_G
Cuts at: 513 1065 2016 2052 2299 3091 3423 4298 513
Size: 552 951 36 247 792 332 875 3074
Fragments arranged by size:
3074 951 875 792 552 332 247 36
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2109 2424 2864 3159 3415 3945 4886
Size: 1255 529 315 440 295 256 530 941
Cuts at: 4886 4903 5056 5241 5686 325
Size: 17 153 185 445 1498
Fragments arranged by size:
1498 1255 941 530 529 445 440 315
295 256 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2375 2613 3204
Size: 274 80 33 51 1443 398 238 591
Cuts at: 3204 5105 6817 96
Size: 1901 1712 138
Fragments arranged by size:
1901 1712 1443 591 398 274 238 138
80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2543 2575 6819 6851 2543
Size: 32 4244 32 2551
Fragments arranged by size:
4244 2551 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2411 3112 1316
Size: 1095 701 5063
Fragments arranged by size:
5063 1095 701
XbaI T'CTAG_A
Cuts at: 4195 4195
Size: 6859
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2663 2663
Size: 6859
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6859
XmaI C'CCGG_G
Cuts at: 1870 4184 1870
Size: 2314 4545
XmnI GAAnn'nnTTC
Cuts at: 2154 4068 4988 2154
Size: 1914 920 4025
Fragments arranged by size:
4025 1914 920
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3620 4667 276
Size: 53 83 186 936 2086 1047 2468
Fragments arranged by size:
2468 2086 1047 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI EcoRV FspI HaeII Hin4I HincII HindIII HpaI
KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI
NcoI NdeI NgoMIV NheI NotI NspI PasI PciI
PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI
PvuI PvuII SacI SalI SapI SbfI ScaI SexAI
SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI
BfrBI BsiWI BstZ17I DraIII FalI FseI FspAI KasI
NarI NruI NsiI PacI PmlI PspOMI PspXI PsrI
RsrII SacII SanDI SfiI SfoI SrfI SwaI