Restriction map for
pFN21AA0054
(Circular) (Six-base) MAPSORT of: pFN21AA0054.seq Check: 4108 from: 1 to: 10531
pFN21AA0054 10531 bp
With 182 enzymes: SgfI *
October 20, 2009 10:35 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 8341 278
Size: 53 83 186 936 6805 2468
Fragments arranged by size:
6805 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2245 6499 7874 1937
Size: 308 4254 1375 4594
Fragments arranged by size:
4594 4254 1375 308
Acc65I G'GTAC_C
Cuts at: 7852 7852
Size: 10531
AclI AA'CG_TT
Cuts at: 1563 3217 4028 8658 9031 1563
Size: 1654 811 4630 373 3063
Fragments arranged by size:
4630 3063 1654 811 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2589 3579 3761 7338 7349 7556 8594 9978
Size: 957 990 182 3577 11 207 1038 1384
Cuts at: 9978 10246 1632
Size: 268 1917
Fragments arranged by size:
3577 1917 1384 1038 990 957 268 207
182 11
AfeI AGC'GCT
Cuts at: 7699 7699
Size: 10531
AflII C'TTAA_G
Cuts at: 820 1017 2054 820
Size: 197 1037 9297
Fragments arranged by size:
9297 1037 197
AflIII A'CryG_T
Cuts at: 1203 2248 3039 4792 9430 10407 1203
Size: 1045 791 1753 4638 977 1327
Fragments arranged by size:
4638 1753 1327 1045 977 791
AgeI A'CCGG_T
Cuts at: 9362 9362
Size: 10531
AhdI GACnn_n'nnGTC
Cuts at: 7762 9260 7762
Size: 1498 9033
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 10531
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2317 4578 1184
Size: 1133 2261 7137
Fragments arranged by size:
7137 2261 1133
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4589 4621 4589
Size: 32 10499
AlwNI CAG_nnn'CTG
Cuts at: 2816 3463 6344 7780 8274 9846 2816
Size: 647 2881 1436 494 1572 3501
Fragments arranged by size:
3501 2881 1572 1436 647 494
ApaI G_GGCC'C
Cuts at: 7711 7711
Size: 10531
ApaLI G'TGCA_C
Cuts at: 8588 9744 8588
Size: 1156 9375
ApoI r'AATT_y
Cuts at: 1493 2737 3303 5189 5321 5521 5993 7840 8066
Size: 1244 566 1886 132 200 472 1847 226
Cuts at: 8066 8218 10327 1493
Size: 152 2109 1697
Fragments arranged by size:
2109 1886 1847 1697 1244 566 472 226
200 152 132
AseI AT'TA_AT
Cuts at: 160 1032 5877 9085 160
Size: 872 4845 3208 1606
Fragments arranged by size:
4845 3208 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 10531
AvaI C'yCGr_G
Cuts at: 1870 1943 5331 6466 7550 7856 1870
Size: 73 3388 1135 1084 306 4545
Fragments arranged by size:
4545 3388 1135 1084 306 73
AvrII C'CTAG_G
Cuts at: 2519 7786 2519
Size: 5267 5264
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2949 2982 5641 5674 2949
Size: 33 2659 33 7806
Fragments arranged by size:
7806 2659 33 33
BamHI G'GATC_C
Cuts at: 3919 7463 7861 8228 3919
Size: 3544 398 367 6222
Fragments arranged by size:
6222 3544 398 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2591 5314 5623 7036 7852
Size: 325 217 627 804 2723 309 1413 816
Cuts at: 7852 9307 618
Size: 1455 1842
Fragments arranged by size:
2723 1842 1455 1413 816 804 627 325
309 217
BanII G_rGCy'C
Cuts at: 1391 5338 7254 7664 7711 7796 7850 1391
Size: 3947 1916 410 47 85 54 4072
Fragments arranged by size:
4072 3947 1916 410 85 54 47
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2016 2174 2478 5297 5393 928
Size: 971 117 158 304 2819 96 6066
Fragments arranged by size:
6066 2819 971 304 158 117 96
BbvCI CC'TCA_GC
Cuts at: 6192 6240 6255 6636 6192
Size: 48 15 381 10087
Fragments arranged by size:
10087 381 48 15
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2155 2189 6551 6585 8722 8756 1903
Size: 34 218 34 4362 34 2137 34 3678
Fragments arranged by size:
4362 3678 2137 218 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2347 5642 8423 9633 2347
Size: 3295 2781 1210 3245
Fragments arranged by size:
3295 3245 2781 1210
BclI T'GATC_A
Cuts at: 1548 4468 4876 5419 6763 1548
Size: 2920 408 543 1344 5316
Fragments arranged by size:
5316 2920 1344 543 408
BfrBI ATG'CAT
Cuts at: 3184 3184
Size: 10531
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2291 7126 9142
Size: 107 122 71 631 605 619 4835 2016
Cuts at: 9142 136
Size: 1525
Fragments arranged by size:
4835 2016 1525 631 619 605 122 107
71
BglII A'GATC_T
Cuts at: 3166 10526 3166
Size: 7360 3171
BlpI GC'TnA_GC
Cuts at: 7479 7948 7479
Size: 469 10062
Bme1580I G_kGCm'C
Cuts at: 1790 2596 7711 8592 9748 1790
Size: 806 5115 881 1156 2573
Fragments arranged by size:
5115 2573 1156 881 806
BmgBI CAC'GTC
Cuts at: 1318 5966 1318
Size: 4648 5883
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2590 3409 4214
Size: 416 53 83 399 24 1173 819 805
Cuts at: 4214 4324 5260 5577 5979 8262 9220 442
Size: 110 936 317 402 2283 958 1753
Fragments arranged by size:
2283 1753 1173 958 936 819 805 416
402 399 317 110 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 10531
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 6481 6531 6481
Size: 50 10481
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3513 6608 9191 1239
Size: 2274 3095 2583 2579
Fragments arranged by size:
3095 2583 2579 2274
Bpu10I CC'TnA_GC
Cuts at: 4041 6192 6240 6255 6636 7734 4041
Size: 2151 48 15 381 1098 6838
Fragments arranged by size:
6838 2151 1098 381 48 15
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2066 4231 6709 7320 8656 9521 9819 2066
Size: 2165 2478 611 1336 865 298 2778
Fragments arranged by size:
2778 2478 2165 1336 865 611 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 6707 9194 882
Size: 617 5208 2487 2219
Fragments arranged by size:
5208 2487 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2467 3040 4895 4994 8322 493
Size: 711 1263 573 1855 99 3328 2702
Fragments arranged by size:
3328 2702 1855 1263 711 573 99
BsaBI GATnn'nnATC
Cuts at: 2910 10525 2910
Size: 7615 2916
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 8338 8720 9355 275
Size: 53 83 186 936 6805 382 635 1451
Fragments arranged by size:
6805 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 5864 6352 8964 9362 9636 9783 10194 5864
Size: 488 2612 398 274 147 411 6201
Fragments arranged by size:
6201 2612 488 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2772 2802 4589 4619 4716
Size: 30 165 30 1369 30 1787 30 97
Cuts at: 4716 4746 5595 5625 6945 6975 1178
Size: 30 849 30 1320 30 4734
Fragments arranged by size:
4734 1787 1369 1320 849 165 97 30
30 30 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3419 4737 5484 6243 6321 7008 7323 1186
Size: 2233 1318 747 759 78 687 315 4394
Fragments arranged by size:
4394 2233 1318 759 747 687 315 78
BseYI C'CCAG_C
Cuts at: 2254 4200 6096 6943 9734 10230 2254
Size: 1946 1896 847 2791 496 2555
Fragments arranged by size:
2791 2555 1946 1896 847 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2074 6327 1163
Size: 573 338 4253 5367
Fragments arranged by size:
5367 4253 573 338
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 7981 8742 9770 1540
Size: 275 186 5980 761 1028 2301
Fragments arranged by size:
5980 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2796 5128 5171 7664 7796 8592 8677 9748 2796
Size: 2332 43 2493 132 796 85 1071 3579
Fragments arranged by size:
3579 2493 2332 1071 796 132 85 43
BsmI GAATG_Cn'
Cuts at: 2883 3731 5350 8042 8135 2883
Size: 848 1619 2692 93 5279
Fragments arranged by size:
5279 2692 1619 848 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2596 2796 5128 5171 5338 7254 7664
Size: 399 806 200 2332 43 167 1916 410
Cuts at: 7664 7711 7796 7850 8592 8677 9748 1391
Size: 47 85 54 742 85 1071 2174
Fragments arranged by size:
2332 2174 1916 1071 806 742 410 399
200 167 85 85 54 47 43
BspEI T'CCGG_A
Cuts at: 5864 6352 10194 5864
Size: 488 3842 6201
Fragments arranged by size:
6201 3842 488
BspHI T'CATG_A
Cuts at: 5218 8420 10150 5218
Size: 3202 1730 5599
Fragments arranged by size:
5599 3202 1730
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 5599 7886 844
Size: 4755 2287 3489
Fragments arranged by size:
4755 3489 2287
BsrBI CCG'CTC
Cuts at: 1394 1823 4605 8418 1394
Size: 429 2782 3813 3507
Fragments arranged by size:
3813 3507 2782 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2894 2931 5157 5706 6405 7115 7465
Size: 1183 1646 37 2226 549 699 710 350
Cuts at: 7465 7813 9026 9200 65
Size: 348 1213 174 1396
Fragments arranged by size:
2226 1646 1396 1213 1183 710 699 549
350 348 174 37
BsrFI r'CCGG_y
Cuts at: 1700 1810 9175 9362 1700
Size: 110 7365 187 2869
Fragments arranged by size:
7365 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 4732 7147 96
Size: 4636 2415 3480
Fragments arranged by size:
4636 3480 2415
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 10531
BssSI C'ACGA_G
Cuts at: 4719 8591 9603 4719
Size: 3872 1012 5647
Fragments arranged by size:
5647 3872 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2942 3506 6062 794
Size: 2148 564 2556 5263
Fragments arranged by size:
5263 2556 2148 564
BstBI TT'CG_AA
Cuts at: 4906 5887 9335 4906
Size: 981 3448 6102
Fragments arranged by size:
6102 3448 981
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 10531
BstXI CCAn_nnnn'nTGG
Cuts at: 4827 5435 4827
Size: 608 9923
BstYI r'GATC_y
Cuts at: 1972 3166 3919 5557 5741 7463 7861 8228 8613
Size: 1194 753 1638 184 1722 398 367 385
Cuts at: 8613 8630 10082 10168 10180 10526 1972
Size: 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1722 1638 1452 1194 753 398 385
367 346 184 86 17 12
BstZ17I GTA'TAC
Cuts at: 6500 6500
Size: 10531
Bsu36I CC'TnA_GG
Cuts at: 7524 7524
Size: 10531
BtgI C'CryG_G
Cuts at: 513 1065 2004 2124 3884 4401 5639 513
Size: 552 939 120 1760 517 1238 5405
Fragments arranged by size:
5405 1760 1238 939 552 517 120
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 9792
Fragments arranged by size:
9792 683 56
BtsI GCAGTG_nn'
Cuts at: 2086 2301 6560 6700 8047 8841 8861 2086
Size: 215 4259 140 1347 794 20 3756
Fragments arranged by size:
4259 3756 1347 794 215 140 20
ClaI AT'CG_AT
Cuts at: 1554 3793 1554
Size: 2239 8292
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2329 2364 3955 3990 529
Size: 35 1765 35 1591 35 7070
Fragments arranged by size:
7070 1765 1591 35 35 35
DraI TTT'AAA
Cuts at: 7828 7835 7828
Size: 7 10524
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 9538 809
Size: 507 8222 1802
Fragments arranged by size:
8222 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2113 2268 3957
Size: 54 1425 50 275 18 283 155 1689
Cuts at: 3957 4662 4817 6320 7594 7638 7978 8867 10496
Size: 705 155 1503 1274 44 340 889 1629
Cuts at: 10496 8
Size: 43
Fragments arranged by size:
1689 1629 1503 1425 1274 889 705 340
283 275 155 155 54 50 44 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 7978 1537
Size: 275 6166 4090
Fragments arranged by size:
6166 4090 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2011 3275 4794 7236 7742 7775 8461 1353
Size: 658 1264 1519 2442 506 33 686 3423
Fragments arranged by size:
3423 2442 1519 1264 686 658 506 33
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2498 9118 9492 9638 387
Size: 1064 138 909 6620 374 146 1280
Fragments arranged by size:
6620 1280 1064 909 374 146 138
EcoICRI GAG'CTC
Cuts at: 7662 7794 7662
Size: 132 10399
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2589 3513 3579 3761 6608 7338 7349
Size: 393 957 924 66 182 2847 730 11
Cuts at: 7349 7556 8594 9191 9978 10246 1239
Size: 207 1038 597 787 268 1524
Fragments arranged by size:
2847 1524 1038 957 924 787 730 597
393 268 207 182 66 11
EcoO109I rG'GnC_Cy
Cuts at: 2098 2141 6281 7259 7708 7725 2098
Size: 43 4140 978 449 17 4904
Fragments arranged by size:
4904 4140 978 449 43 17
EcoRI G'AATT_C
Cuts at: 2737 7840 8218 2737
Size: 5103 378 5050
Fragments arranged by size:
5103 5050 378
EcoRV GAT'ATC
Cuts at: 2462 2462
Size: 10531
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2667 4307 5022 5063 2667
Size: 1640 715 41 8135
Fragments arranged by size:
8135 1640 715 41
FspI TGC'GCA
Cuts at: 9037 9037
Size: 10531
HaeII r_GCGC'y
Cuts at: 1678 6961 7502 7701 9678 1678
Size: 5283 541 199 1977 2531
Fragments arranged by size:
5283 2531 1977 541 199
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2471 2503 2839 2871 3215
Size: 32 135 32 899 32 336 32 344
Cuts at: 3215 3247 3355 3387 3786 3818 5918 5950 6175
Size: 32 108 32 399 32 2100 32 225
Cuts at: 6175 6207 6550 6582 7748 7780 9172 9204 9246
Size: 32 343 32 1166 32 1392 32 42
Cuts at: 9246 9278 1373
Size: 32 2626
Fragments arranged by size:
2626 2100 1392 1166 899 399 344 343
336 225 135 108 42 32 32 32
32 32 32 32 32 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 7875 8121 669
Size: 988 281 5937 246 3079
Fragments arranged by size:
5937 3079 988 281 246
HindIII A'AGCT_T
Cuts at: 748 3469 3539 5099 748
Size: 2721 70 1560 6180
Fragments arranged by size:
6180 2721 1560 70
HpaI GTT'AAC
Cuts at: 8121 8121
Size: 10531
KpnI G_GTAC'C
Cuts at: 7856 7856
Size: 10531
MfeI C'AATT_G
Cuts at: 3452 8130 3452
Size: 4678 5853
MluI A'CGCG_T
Cuts at: 10407 10407
Size: 10531
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2353 3897 4326 7521 9645 9829 1305
Size: 1048 1544 429 3195 2124 184 2007
Fragments arranged by size:
3195 2124 2007 1544 1048 429 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2115 2270 3959 4664 4819
Size: 54 1425 343 283 155 1689 705 155
Cuts at: 4819 7596 10
Size: 2777 2945
Fragments arranged by size:
2945 2777 1689 1425 705 343 283 155
155 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3866 4143 6507 7167 8489 8848 9007
Size: 648 2700 277 2364 660 1322 359 159
Cuts at: 9007 518
Size: 2042
Fragments arranged by size:
2700 2364 2042 1322 660 648 359 277
159
MspA1I CmG'CkG
Cuts at: 1606 2314 3985 5311 7947 8624 9772 10017 1606
Size: 708 1671 1326 2636 677 1148 245 2120
Fragments arranged by size:
2636 2120 1671 1326 1148 708 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 2124 3884 4401 5639 513
Size: 552 939 120 1760 517 1238 5405
Fragments arranged by size:
5405 1760 1238 939 552 517 120
NdeI CA'TA_TG
Cuts at: 387 4144 4870 5296 387
Size: 3757 726 426 5622
Fragments arranged by size:
5622 3757 726 426
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 10531
NotI GC'GGCC_GC
Cuts at: 7978 7978
Size: 10531
NsiI A_TGCA'T
Cuts at: 3186 3186
Size: 10531
NspI r_CATG'y
Cuts at: 1132 1229 2046 2252 2311 3188 4477 7172 7889
Size: 97 817 206 59 877 1289 2695 717
Cuts at: 7889 9434 1132
Size: 1545 2229
Fragments arranged by size:
2695 2229 1545 1289 877 817 717 206
97 59
PasI CC'CwG_GG
Cuts at: 1348 3646 6646 1348
Size: 2298 3000 5233
Fragments arranged by size:
5233 3000 2298
PciI A'CATG_T
Cuts at: 2248 9430 2248
Size: 7182 3349
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3885 4402 1108
Size: 2777 517 7237
Fragments arranged by size:
7237 2777 517
PfoI T'CCnGG_A
Cuts at: 7773 7773
Size: 10531
PmeI GTTT'AAAC
Cuts at: 7835 7835
Size: 10531
PmlI CAC'GTG
Cuts at: 2467 2467
Size: 10531
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3111 3143 4589 4621 8600 8632 10113 10145 3111
Size: 32 1446 32 3979 32 1481 32 3497
Fragments arranged by size:
3979 3497 1481 1446 32 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 6281 7259 6281
Size: 978 9553
PshAI GACnn'nnGTC
Cuts at: 1613 3593 1613
Size: 1980 8551
PsiI TTA'TAA
Cuts at: 4761 8101 4761
Size: 3340 7191
PspOMI G'GGCC_C
Cuts at: 7707 7707
Size: 10531
PspXI vC'TCGA_Gb
Cuts at: 7550 7550
Size: 10531
PstI C_TGCA'G
Cuts at: 830 4065 5349 6340 7587 7883 830
Size: 3235 1284 991 1247 296 3478
Fragments arranged by size:
3478 3235 1284 1247 991 296
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 10531
PvuII CAG'CTG
Cuts at: 2314 3985 2314
Size: 1671 8860
SacI G_AGCT'C
Cuts at: 7664 7796 7664
Size: 132 10399
SalI G'TCGA_C
Cuts at: 1936 7873 1936
Size: 5937 4594
SanDI GG'GwC_CC
Cuts at: 6281 6281
Size: 10531
SapI GCTCTTCn'nnn_
Cuts at: 2011 3275 2011
Size: 1264 9267
SbfI CC_TGCA'GG
Cuts at: 7883 7883
Size: 10531
ScaI AGT'ACT
Cuts at: 2120 2956 3126 4235 8779 10491 2120
Size: 836 170 1109 4544 1712 2160
Fragments arranged by size:
4544 2160 1712 1109 836 170
SexAI A'CCwGG_T
Cuts at: 7261 7261
Size: 10531
SfcI C'TryA_G
Cuts at: 826 1045 2188 2581 3159 4061 5345 6336 6477
Size: 219 1143 393 578 902 1284 991 141
Cuts at: 6477 7573 7583 7879 9014 9695 9886 826
Size: 1096 10 296 1135 681 191 1471
Fragments arranged by size:
1471 1284 1143 1135 1096 991 902 681
578 393 296 219 191 141 10
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 10531
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 10531
SmaI CCC'GGG
Cuts at: 1872 5333 7858 1872
Size: 3461 2525 4545
Fragments arranged by size:
4545 3461 2525
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2054 2081 4246 6724 7335 7550
Size: 197 926 111 27 2165 2478 611 215
Cuts at: 7550 8635 9536 9798 820
Size: 1085 901 262 1553
Fragments arranged by size:
2478 2165 1553 1085 926 901 611 262
215 197 111 27
SnaBI TAC'GTA
Cuts at: 493 4994 8322 493
Size: 4501 3328 2702
Fragments arranged by size:
4501 3328 2702
SpeI A'CTAG_T
Cuts at: 152 2441 152
Size: 2289 8242
SphI G_CATG'C
Cuts at: 1132 2311 3188 7889 1132
Size: 1179 877 4701 3774
Fragments arranged by size:
4701 3774 1179 877
SspI AAT'ATT
Cuts at: 5 52 3075 8455 5
Size: 47 3023 5380 2081
Fragments arranged by size:
5380 3023 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 10531
StyI C'CwwG_G
Cuts at: 513 1065 2004 2124 2271 2519 3884 4401 5388
Size: 552 939 120 147 248 1365 517 987
Cuts at: 5388 5639 5761 7786 7970 513
Size: 251 122 2025 184 3074
Fragments arranged by size:
3074 2025 1365 987 939 552 517 251
248 184 147 122 120
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4109 4233 4557 5138 5401 5532 5787
Size: 1255 2529 124 324 581 263 131 255
Cuts at: 5787 7209 7519 8558 8575 8728 8913 9358 325
Size: 1422 310 1039 17 153 185 445 1498
Fragments arranged by size:
2529 1498 1422 1255 1039 581 445 324
310 263 255 185 153 131 124 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2118 2600 2954
Size: 274 80 33 51 1443 141 482 354
Cuts at: 2954 3124 3637 4233 4732 5461 6832 6875 7147
Size: 170 513 596 499 729 1371 43 272
Cuts at: 7147 8777 10489 96
Size: 1630 1712 138
Fragments arranged by size:
1712 1630 1443 1371 729 596 513 499
482 354 274 272 170 141 138 80
51 43 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2451 2483 2961 2993 3567 3599 3963 3995 6840
Size: 32 478 32 574 32 364 32 2845
Cuts at: 6840 6872 6945 6977 10491 10523 2451
Size: 32 73 32 3514 32 2459
Fragments arranged by size:
3514 2845 2459 574 478 364 73 32
32 32 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 7263 1316
Size: 5947 4584
XbaI T'CTAG_A
Cuts at: 7867 7867
Size: 10531
XhoI C'TCGA_G
Cuts at: 1943 7550 1943
Size: 5607 4924
XmaI C'CCGG_G
Cuts at: 1870 5331 7856 1870
Size: 3461 2525 4545
Fragments arranged by size:
4545 3461 2525
XmnI GAAnn'nnTTC
Cuts at: 4287 6446 8660 4287
Size: 2159 2214 6158
Fragments arranged by size:
6158 2214 2159
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 8339 276
Size: 53 83 186 936 6805 2468
Fragments arranged by size:
6805 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI AvrII BaeI BamHI BanI
BanII BbsI BbvCI BcgI BciVI BclI BfrBI BglI
BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstXI BstYI BstZ17I Bsu36I BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoO109I EcoRI EcoRV FalI
FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI
MluI MmeI MscI MslI MspA1I NcoI NdeI NheI
NotI NsiI NspI PasI PciI PflMI PfoI PmeI
PmlI PpiI PpuMI PshAI PsiI PspOMI PspXI PstI
PvuI PvuII SacI SalI SanDI SapI SbfI ScaI
SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AscI BbeI BsiWI BsmBI DraIII EcoNI FseI
FspAI KasI NaeI NarI NgoMIV NruI PacI PsrI
RsrII SacII SfiI SfoI SrfI SwaI XcmI