Restriction map for
pFN21AA0044
(Circular) (Six-base) MAPSORT of: pFN21AA0044.seq Check: 1358 from: 1 to: 6130
pFN21AA0044 6130 bp
With 182 enzymes: SgfI *
February 27, 2009 14:36 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3940 278
Size: 53 83 186 936 2404 2468
Fragments arranged by size:
2468 2404 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2760 3473 1937
Size: 823 713 4594
Fragments arranged by size:
4594 823 713
Acc65I G'GTAC_C
Cuts at: 3451 3451
Size: 6130
AclI AA'CG_TT
Cuts at: 1563 2351 4257 4630 1563
Size: 788 1906 373 3063
Fragments arranged by size:
3063 1906 788 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3176 4193 5577 5845 1632
Size: 1544 1017 1384 268 1917
Fragments arranged by size:
1917 1544 1384 1017 268
AflII C'TTAA_G
Cuts at: 820 1017 2398 820
Size: 197 1381 4552
Fragments arranged by size:
4552 1381 197
AflIII A'CryG_T
Cuts at: 1203 2280 5029 6006 1203
Size: 1077 2749 977 1327
Fragments arranged by size:
2749 1327 1077 977
AgeI A'CCGG_T
Cuts at: 4961 4961
Size: 6130
AhdI GACnn_n'nnGTC
Cuts at: 2865 4859 2865
Size: 1994 4136
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6130
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6130
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2940 2972 2940
Size: 32 6098
AlwNI CAG_nnn'CTG
Cuts at: 3873 5445 3873
Size: 1572 4558
ApaLI G'TGCA_C
Cuts at: 4187 5343 4187
Size: 1156 4974
ApoI r'AATT_y
Cuts at: 1493 2324 2390 2549 2930 3439 3665 3817 5926
Size: 831 66 159 381 509 226 152 2109
Cuts at: 5926 1493
Size: 1697
Fragments arranged by size:
2109 1697 831 509 381 226 159 152
66
AseI AT'TA_AT
Cuts at: 160 1032 2670 4684 160
Size: 872 1638 2014 1606
Fragments arranged by size:
2014 1638 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6130
AvaI C'yCGr_G
Cuts at: 1870 1943 2502 3455 1870
Size: 73 559 953 4545
Fragments arranged by size:
4545 953 559 73
AvrII C'CTAG_G
Cuts at: 2554 2554
Size: 6130
BamHI G'GATC_C
Cuts at: 3460 3827 3460
Size: 367 5763
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3451 4906 618
Size: 325 217 627 1664 1455 1842
Fragments arranged by size:
1842 1664 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2484 3013 3449 1391
Size: 1093 529 436 4072
Fragments arranged by size:
4072 1093 529 436
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5159
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3055 3089 4321 4355 1903
Size: 34 1118 34 1232 34 3678
Fragments arranged by size:
3678 1232 1118 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4022 5232 4022
Size: 1210 4920
BclI T'GATC_A
Cuts at: 1548 2083 2232 2452 1548
Size: 535 149 220 5226
Fragments arranged by size:
5226 535 220 149
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4741 136
Size: 107 122 71 631 605 3069 1525
Fragments arranged by size:
3069 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6125 6125
Size: 6130
BlpI GC'TnA_GC
Cuts at: 3547 3547
Size: 6130
Bme1580I G_kGCm'C
Cuts at: 1790 4191 5347 1790
Size: 2401 1156 2573
Fragments arranged by size:
2573 2401 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6130
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3861 4819 442
Size: 416 53 83 399 24 2444 958 1753
Fragments arranged by size:
2444 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6130
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3430 3430
Size: 6130
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2400 2965 4790 1239
Size: 1161 565 1825 2579
Fragments arranged by size:
2579 1825 1161 565
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2581 4255 5120 5418 2581
Size: 1674 865 298 3293
Fragments arranged by size:
3293 1674 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4793 882
Size: 617 3294 2219
Fragments arranged by size:
3294 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3921 493
Size: 711 2717 2702
Fragments arranged by size:
2717 2702 711
BsaBI GATnn'nnATC
Cuts at: 6124 6124
Size: 6130
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3937 4319 4954 275
Size: 53 83 186 936 2404 382 635 1451
Fragments arranged by size:
2404 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4563 4961 5235 5382 5793 4563
Size: 398 274 147 411 4900
Fragments arranged by size:
4900 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5905
Fragments arranged by size:
5905 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 6130
BseYI C'CCAG_C
Cuts at: 2769 5333 5829 2769
Size: 2564 496 3070
Fragments arranged by size:
3070 2564 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2810 1163
Size: 573 1074 4483
Fragments arranged by size:
4483 1074 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3427 3580 4341 5369 1540
Size: 275 186 1426 153 761 1028 2301
Fragments arranged by size:
2301 1426 1028 761 275 186 153
BsiHKAI G_wGCw'C
Cuts at: 2484 2703 3013 4191 4276 5347 2484
Size: 219 310 1178 85 1071 3267
Fragments arranged by size:
3267 1178 1071 310 219 85
BsmI GAATG_Cn'
Cuts at: 3641 3734 3641
Size: 93 6037
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2484 2703 3013 3449 4191 4276 5347
Size: 399 694 219 310 436 742 85 1071
Cuts at: 5347 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 694 436 399 310 219
85
BspEI T'CCGG_A
Cuts at: 5793 5793
Size: 6130
BspHI T'CATG_A
Cuts at: 3039 4019 5749 3039
Size: 980 1730 3420
Fragments arranged by size:
3420 1730 980
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3485 844
Size: 2641 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2321 4017 1394
Size: 429 498 1696 3507
Fragments arranged by size:
3507 1696 498 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4625 4799 65
Size: 1183 3377 174 1396
Fragments arranged by size:
3377 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4774 4961 1700
Size: 110 2964 187 2869
Fragments arranged by size:
2964 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 3197 96
Size: 3101 3029
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6130
BssSI C'ACGA_G
Cuts at: 4190 5202 4190
Size: 1012 5118
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6130
BstBI TT'CG_AA
Cuts at: 2287 4934 2287
Size: 2647 3483
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6130
BstXI CCAn_nnnn'nTGG
Cuts at: 2011 2011
Size: 6130
BstYI r'GATC_y
Cuts at: 1972 2497 3310 3460 3827 4212 4229 5681 5767
Size: 525 813 150 367 385 17 1452 86
Cuts at: 5767 5779 6125 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 813 525 385 367 346 150
86 17 12
Bsu36I CC'TnA_GG
Cuts at: 3357 3357
Size: 6130
BtgI C'CryG_G
Cuts at: 513 1065 2004 2046 513
Size: 552 939 42 4597
Fragments arranged by size:
4597 939 552 42
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5391
Fragments arranged by size:
5391 683 56
BtsI GCAGTG_nn'
Cuts at: 3391 3646 4440 4460 3391
Size: 255 794 20 5061
Fragments arranged by size:
5061 794 255 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6130
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6095
DraI TTT'AAA
Cuts at: 3434 3434
Size: 6130
DraIII CAC_nnn'GTG
Cuts at: 2156 2743 2156
Size: 587 5543
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5137 809
Size: 507 3821 1802
Fragments arranged by size:
3821 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2019 2852 2967
Size: 54 1425 50 275 18 189 833 115
Cuts at: 2967 3424 3577 4466 6095 8
Size: 457 153 889 1629 43
Fragments arranged by size:
1629 1425 889 833 457 275 189 153
115 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3424 3577 1537
Size: 275 1612 153 4090
Fragments arranged by size:
4090 1612 275 153
EarI CTCTTCn'nnn_
Cuts at: 1353 2691 2895 2960 3167 4060 1353
Size: 1338 204 65 207 893 3423
Fragments arranged by size:
3423 1338 893 207 204 65
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4717 5091 5237 387
Size: 1064 138 3128 374 146 1280
Fragments arranged by size:
3128 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2482 3011 2482
Size: 529 5601
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2400 2965 3176 4193 4790 5577 5845
Size: 393 768 565 211 1017 597 787 268
Cuts at: 5845 1239
Size: 1524
Fragments arranged by size:
1524 1017 787 768 597 565 393 268
211
EcoO109I rG'GnC_Cy
Cuts at: 3361 3361
Size: 6130
EcoRI G'AATT_C
Cuts at: 3439 3817 3439
Size: 378 5752
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2111 2728 3397 2111
Size: 617 669 4844
Fragments arranged by size:
4844 669 617
FspI TGC'GCA
Cuts at: 4636 4636
Size: 6130
HaeII r_GCGC'y
Cuts at: 1678 5277 1678
Size: 3599 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2556 2588 2851 2883 4771
Size: 32 135 32 984 32 263 32 1888
Cuts at: 4771 4803 4845 4877 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1888 984 263 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2278 3474 3720 669
Size: 988 281 340 1196 246 3079
Fragments arranged by size:
3079 1196 988 340 281 246
HindIII A'AGCT_T
Cuts at: 748 2093 748
Size: 1345 4785
HpaI GTT'AAC
Cuts at: 2278 3720 2278
Size: 1442 4688
KpnI G_GTAC'C
Cuts at: 3455 3455
Size: 6130
MfeI C'AATT_G
Cuts at: 3729 3729
Size: 6130
MluI A'CGCG_T
Cuts at: 6006 6006
Size: 6130
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2449 5244 5428 1305
Size: 1144 2795 184 2007
Fragments arranged by size:
2795 2007 1144 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2854 2969 10
Size: 54 1425 343 1022 115 3171
Fragments arranged by size:
3171 1425 1022 343 115 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2009 2628 3170 4088 4447 4606 518
Size: 648 843 619 542 918 359 159 2042
Fragments arranged by size:
2042 918 843 648 619 542 359 159
MspA1I CmG'CkG
Cuts at: 1606 2773 3546 4223 5371 5616 1606
Size: 1167 773 677 1148 245 2120
Fragments arranged by size:
2120 1167 1148 773 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4639
Fragments arranged by size:
4639 939 552
NdeI CA'TA_TG
Cuts at: 387 2265 387
Size: 1878 4252
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6130
NotI GC'GGCC_GC
Cuts at: 3577 3577
Size: 6130
NspI r_CATG'y
Cuts at: 1132 1229 2284 3488 5033 1132
Size: 97 1055 1204 1545 2229
Fragments arranged by size:
2229 1545 1204 1055 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6130
PciI A'CATG_T
Cuts at: 2280 5029 2280
Size: 2749 3381
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2047 1108
Size: 939 5191
PmeI GTTT'AAAC
Cuts at: 3434 3434
Size: 6130
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4199 4231 5712 5744 4199
Size: 32 1481 32 4585
Fragments arranged by size:
4585 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 3361 3361
Size: 6130
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6130
PsiI TTA'TAA
Cuts at: 3700 3700
Size: 6130
PstI C_TGCA'G
Cuts at: 830 3398 3482 830
Size: 2568 84 3478
Fragments arranged by size:
3478 2568 84
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6130
PvuII CAG'CTG
Cuts at: 2773 2773
Size: 6130
SacI G_AGCT'C
Cuts at: 2484 3013 2484
Size: 529 5601
SalI G'TCGA_C
Cuts at: 1936 3472 1936
Size: 1536 4594
SapI GCTCTTCn'nnn_
Cuts at: 2691 3167 2691
Size: 476 5654
SbfI CC_TGCA'GG
Cuts at: 3482 3482
Size: 6130
ScaI AGT'ACT
Cuts at: 4378 6090 4378
Size: 1712 4418
SfcI C'TryA_G
Cuts at: 826 1045 2375 3394 3478 4613 5294 5485 826
Size: 219 1330 1019 84 1135 681 191 1471
Fragments arranged by size:
1471 1330 1135 1019 681 219 191 84
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6130
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6130
SmaI CCC'GGG
Cuts at: 1872 3457 1872
Size: 1585 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2398 2560 4234 5135 5397 820
Size: 197 926 455 162 1674 901 262 1553
Fragments arranged by size:
1674 1553 926 901 455 262 197 162
SnaBI TAC'GTA
Cuts at: 493 3921 493
Size: 3428 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6130
SphI G_CATG'C
Cuts at: 1132 3488 1132
Size: 2356 3774
SspI AAT'ATT
Cuts at: 5 52 2655 4054 5
Size: 47 2603 1399 2081
Fragments arranged by size:
2603 2081 1399 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6130
StyI C'CwwG_G
Cuts at: 513 1065 2004 2554 3381 3569 513
Size: 552 939 550 827 188 3074
Fragments arranged by size:
3074 939 827 552 550 188
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2231 4157 4174 4327 4512 4957 325
Size: 1255 651 1926 17 153 185 445 1498
Fragments arranged by size:
1926 1498 1255 651 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3197 4376 6088
Size: 274 80 33 51 1443 1220 1179 1712
Cuts at: 6088 96
Size: 138
Fragments arranged by size:
1712 1443 1220 1179 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6090 6122 6090
Size: 32 6098
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6130
XbaI T'CTAG_A
Cuts at: 3466 3466
Size: 6130
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3379 3379
Size: 6130
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6130
XmaI C'CCGG_G
Cuts at: 1870 3455 1870
Size: 1585 4545
XmnI GAAnn'nnTTC
Cuts at: 3161 4259 3161
Size: 1098 5032
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3938 276
Size: 53 83 186 936 2404 2468
Fragments arranged by size:
2468 2404 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI
AsiSI AvaI AvrII BamHI BanI BanII BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I
BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI
MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI
NheI NotI NspI PasI PciI PflMI PmeI PpiI
PpuMI PshAI PsiI PstI PvuI PvuII SacI SalI
SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI ApaI AscI BaeI BbeI BbvCI BfrBI
Bpu10I BsiWI BsmBI BstZ17I EcoNI EcoRV FseI FspAI
KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI
PmlI PspOMI PspXI PsrI RsrII SacII SanDI SexAI
SfiI SfoI SrfI SwaI