Restriction map for
pFN21AA0034
(Circular) (Six-base) MAPSORT of: pFN21AA0034.seq Check: 7150 from: 1 to: 9730
pFN21AA0034 9730 bp
With 182 enzymes: SgfI *
April 15, 2010 09:24 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 7540 278
Size: 53 83 186 936 6004 2468
Fragments arranged by size:
6004 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4435 7073 1937
Size: 2498 2638 4594
Fragments arranged by size:
4594 2638 2498
Acc65I G'GTAC_C
Cuts at: 2873 7051 2873
Size: 4178 5552
AclI AA'CG_TT
Cuts at: 1563 2645 5305 7857 8230 1563
Size: 1082 2660 2552 373 3063
Fragments arranged by size:
3063 2660 2552 1082 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2068 2252 3105 3233 3308 3405 3734 3753
Size: 436 184 853 128 75 97 329 19
Cuts at: 3753 4500 4980 5226 6675 6834 7793 9177 9445
Size: 747 480 246 1449 159 959 1384 268
Cuts at: 9445 1632
Size: 1917
Fragments arranged by size:
1917 1449 1384 959 853 747 480 436
329 268 246 184 159 128 97 75
19
AflII C'TTAA_G
Cuts at: 820 1017 4292 820
Size: 197 3275 6258
Fragments arranged by size:
6258 3275 197
AflIII A'CryG_T
Cuts at: 1203 5117 5119 8629 9606 1203
Size: 3914 2 3510 977 1327
Fragments arranged by size:
3914 3510 1327 977 2
AgeI A'CCGG_T
Cuts at: 8561 8561
Size: 9730
AhdI GACnn_n'nnGTC
Cuts at: 8459 8459
Size: 9730
AleI CACnn'nnGTG
Cuts at: 1166 5706 6303 1166
Size: 4540 597 4593
Fragments arranged by size:
4593 4540 597
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3708 4898 1184
Size: 2524 1190 6016
Fragments arranged by size:
6016 2524 1190
AlwNI CAG_nnn'CTG
Cuts at: 2017 2056 4039 4096 4976 6442 7473 9045 2017
Size: 39 1983 57 880 1466 1031 1572 2702
Fragments arranged by size:
2702 1983 1572 1466 1031 880 57 39
ApaLI G'TGCA_C
Cuts at: 4184 7787 8943 4184
Size: 3603 1156 4971
Fragments arranged by size:
4971 3603 1156
ApoI r'AATT_y
Cuts at: 1493 2111 3104 3174 4285 4599 4813 5225 5567
Size: 618 993 70 1111 314 214 412 342
Cuts at: 5567 5798 5981 6242 7039 7265 7417 9526 1493
Size: 231 183 261 797 226 152 2109 1697
Fragments arranged by size:
2109 1697 1111 993 797 618 412 342
314 261 231 226 214 183 152 70
AseI AT'TA_AT
Cuts at: 160 1032 4797 8284 160
Size: 872 3765 3487 1606
Fragments arranged by size:
3765 3487 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 9730
AvaI C'yCGr_G
Cuts at: 1870 1943 5496 7055 1870
Size: 73 3553 1559 4545
Fragments arranged by size:
4545 3553 1559 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2044 2077 3244 3277 2044
Size: 33 1167 33 8497
Fragments arranged by size:
8497 1167 33 33
BamHI G'GATC_C
Cuts at: 4151 4858 7060 7427 4151
Size: 707 2202 367 6454
Fragments arranged by size:
6454 2202 707 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2454 2873 6961 7051 8506
Size: 325 217 627 667 419 4088 90 1455
Cuts at: 8506 618
Size: 1842
Fragments arranged by size:
4088 1842 1455 667 627 419 325 217
90
BanII G_rGCy'C
Cuts at: 1391 3838 5941 7049 1391
Size: 2447 2103 1108 4072
Fragments arranged by size:
4072 2447 2103 1108
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2200 2746 3993 928
Size: 971 301 546 1247 6665
Fragments arranged by size:
6665 1247 971 546 301
BbvCI CC'TCA_GC
Cuts at: 4545 4545
Size: 9730
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 7921 7955 1903
Size: 34 5984 34 3678
Fragments arranged by size:
5984 3678 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3272 3524 7622 8832 3272
Size: 252 4098 1210 4170
Fragments arranged by size:
4170 4098 1210 252
BclI T'GATC_A
Cuts at: 1548 5901 1548
Size: 4353 5377
BfrBI ATG'CAT
Cuts at: 3775 3775
Size: 9730
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 8341 136
Size: 107 122 71 631 605 6669 1525
Fragments arranged by size:
6669 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 9725 9725
Size: 9730
BlpI GC'TnA_GC
Cuts at: 6492 7147 6492
Size: 655 9075
Bme1580I G_kGCm'C
Cuts at: 1790 4188 7791 8947 1790
Size: 2398 3603 1156 2573
Fragments arranged by size:
3603 2573 2398 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 9730
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3401 4227 5341
Size: 416 53 83 399 24 1984 826 1114
Cuts at: 5341 5461 5933 6910 6933 7461 8419 442
Size: 120 472 977 23 528 958 1753
Fragments arranged by size:
1984 1753 1114 977 958 826 528 472
416 399 120 83 53 24 23
BmtI G_CTAG'C
Cuts at: 1056 2235 2604 1056
Size: 1179 369 8182
Fragments arranged by size:
8182 1179 369
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2027 2033 2413 2697 2748 3171 3522 5048
Size: 788 6 380 284 51 423 351 1526
Cuts at: 5048 5347 6032 8390 1239
Size: 299 685 2358 2579
Fragments arranged by size:
2579 2358 1526 788 685 423 380 351
299 284 51 6
Bpu10I CC'TnA_GC
Cuts at: 4272 4545 4639 4272
Size: 273 94 9363
Fragments arranged by size:
9363 273 94
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2885 3768 3779 5951 7855 8720 9018 2885
Size: 883 11 2172 1904 865 298 3597
Fragments arranged by size:
3597 2172 1904 883 865 298 11
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4139 4900 8393 882
Size: 617 2640 761 3493 2219
Fragments arranged by size:
3493 2640 2219 761 617
BsaAI yAC'GTr
Cuts at: 493 1204 3065 5120 7521 493
Size: 711 1861 2055 2401 2702
Fragments arranged by size:
2702 2401 2055 1861 711
BsaBI GATnn'nnATC
Cuts at: 4746 9724 4746
Size: 4978 4752
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 7537 7919 8554 275
Size: 53 83 186 936 6004 382 635 1451
Fragments arranged by size:
6004 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 5220 8163 8561 8835 8982 9393 5220
Size: 2943 398 274 147 411 5557
Fragments arranged by size:
5557 2943 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3341 3371 1178
Size: 30 165 30 1938 30 7537
Fragments arranged by size:
7537 1938 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3231 4631 1186
Size: 2045 1400 6285
Fragments arranged by size:
6285 2045 1400
BseYI C'CCAG_C
Cuts at: 2068 2255 3217 3222 8933 9429 2068
Size: 187 962 5 5711 496 2369
Fragments arranged by size:
5711 2369 962 496 187 5
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2502 2907 3939 6327 1163
Size: 573 766 405 1032 2388 4566
Fragments arranged by size:
4566 2388 1032 766 573 405
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 7180 7941 8969 1540
Size: 275 186 5179 761 1028 2301
Fragments arranged by size:
5179 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 4188 4371 5941 6009 7791 7876 8947 4188
Size: 183 1570 68 1782 85 1071 4971
Fragments arranged by size:
4971 1782 1570 1071 183 85 68
BsmI GAATG_Cn'
Cuts at: 3695 7241 7334 3695
Size: 3546 93 6091
Fragments arranged by size:
6091 3546 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 3838 4188 4371 5941 6009 7049 7791
Size: 399 2048 350 183 1570 68 1040 742
Cuts at: 7791 7876 8947 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 2048 1570 1071 1040 742 399 350
183 85 68
BspEI T'CCGG_A
Cuts at: 5220 9393 5220
Size: 4173 5557
BspHI T'CATG_A
Cuts at: 2220 2929 4072 4603 4989 6810 7619 9349 2220
Size: 709 1143 531 386 1821 809 1730 2601
Fragments arranged by size:
2601 1821 1730 1143 809 709 531 386
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2885 5326 7085 844
Size: 2041 2441 1759 3489
Fragments arranged by size:
3489 2441 2041 1759
BsrBI CCG'CTC
Cuts at: 1394 1823 7617 1394
Size: 429 5794 3507
Fragments arranged by size:
5794 3507 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2799 4813 5194 5317 6621 8225 8399
Size: 1183 1551 2014 381 123 1304 1604 174
Cuts at: 8399 65
Size: 1396
Fragments arranged by size:
2014 1604 1551 1396 1304 1183 381 174
123
BsrFI r'CCGG_y
Cuts at: 1700 1810 3077 8374 8561 1700
Size: 110 1267 5297 187 2869
Fragments arranged by size:
5297 2869 1267 187 110
BsrGI T'GTAC_A
Cuts at: 96 4414 4927 5655 5836 96
Size: 4318 513 728 181 3990
Fragments arranged by size:
4318 3990 728 513 181
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 9730
BssSI C'ACGA_G
Cuts at: 6303 7790 8802 6303
Size: 1487 1012 7231
Fragments arranged by size:
7231 1487 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 9730
BstBI TT'CG_AA
Cuts at: 2191 8534 2191
Size: 6343 3387
BstEII G'GTnAC_C
Cuts at: 1184 5609 1184
Size: 4425 5305
BstXI CCAn_nnnn'nTGG
Cuts at: 4869 5606 4869
Size: 737 8993
BstYI r'GATC_y
Cuts at: 1972 2343 4151 4741 4858 7060 7427 7812 7829
Size: 371 1808 590 117 2202 367 385 17
Cuts at: 7829 9281 9367 9379 9725 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
2202 1977 1808 1452 590 385 371 367
346 117 86 17 12
Bsu36I CC'TnA_GG
Cuts at: 3443 3443
Size: 9730
BtgI C'CryG_G
Cuts at: 513 1065 2004 6287 513
Size: 552 939 4283 3956
Fragments arranged by size:
4283 3956 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 8991
Fragments arranged by size:
8991 683 56
BtsI GCAGTG_nn'
Cuts at: 2751 3692 3927 5094 6674 7246 8040 8060 2751
Size: 941 235 1167 1580 572 794 20 4421
Fragments arranged by size:
4421 1580 1167 941 794 572 235 20
ClaI AT'CG_AT
Cuts at: 1554 4342 1554
Size: 2788 6942
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 5425 5460 529
Size: 35 4861 35 4799
Fragments arranged by size:
4861 4799 35 35
DraI TTT'AAA
Cuts at: 4192 7034 4192
Size: 2842 6888
DraIII CAC_nnn'GTG
Cuts at: 3434 3434
Size: 9730
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2101 8737 809
Size: 507 785 6636 1802
Fragments arranged by size:
6636 1802 785 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 4774 7177 8066
Size: 54 1425 50 275 18 2944 2403 889
Cuts at: 8066 9695 8
Size: 1629 43
Fragments arranged by size:
2944 2403 1629 1425 889 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 7177 1537
Size: 275 5365 4090
Fragments arranged by size:
5365 4090 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2271 3618 4730 4826 5889 6551 7660 1353
Size: 918 1347 1112 96 1063 662 1109 3423
Fragments arranged by size:
3423 1347 1112 1109 1063 918 662 96
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 8317 8691 8837 387
Size: 1064 138 6728 374 146 1280
Fragments arranged by size:
6728 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 5939 5939
Size: 9730
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2027 2033 2068 2252 2413 2697 2748
Size: 393 395 6 35 184 161 284 51
Cuts at: 2748 3105 3171 3233 3308 3405 3522 3734 3753
Size: 357 66 62 75 97 117 212 19
Cuts at: 3753 4500 4980 5048 5226 5347 6032 6675 6834
Size: 747 480 68 178 121 685 643 159
Cuts at: 6834 7793 8390 9177 9445 1239
Size: 959 597 787 268 1524
Fragments arranged by size:
1524 959 787 747 685 643 597 480
395 393 357 284 268 212 184 178
161 159 121 117 97 75 68 66
62 51 35 19 6
EcoNI CCTnn'n_nnAGG
Cuts at: 6605 6605
Size: 9730
EcoO109I rG'GnC_Cy
Cuts at: 3737 4174 4954 6935 3737
Size: 437 780 1981 6532
Fragments arranged by size:
6532 1981 780 437
EcoRI G'AATT_C
Cuts at: 3174 4599 4813 5798 6242 7039 7417 3174
Size: 1425 214 985 444 797 378 5487
Fragments arranged by size:
5487 1425 985 797 444 378 214
EcoRV GAT'ATC
Cuts at: 6184 6184
Size: 9730
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3647 4914 6089 6617 6838 3647
Size: 1267 1175 528 221 6539
Fragments arranged by size:
6539 1267 1175 528 221
FspI TGC'GCA
Cuts at: 8236 8236
Size: 9730
HaeII r_GCGC'y
Cuts at: 1678 2217 8877 1678
Size: 539 6660 2531
Fragments arranged by size:
6660 2531 539
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 5393 5425 6130 6162 6467
Size: 32 135 32 3821 32 705 32 305
Cuts at: 6467 6499 8371 8403 8445 8477 1373
Size: 32 1872 32 42 32 2626
Fragments arranged by size:
3821 2626 1872 705 305 135 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3961 4086 4162 4436 4471 5333
Size: 988 281 2023 125 76 274 35 862
Cuts at: 5333 7074 7320 669
Size: 1741 246 3079
Fragments arranged by size:
3079 2023 1741 988 862 281 274 246
125 76 35
HindIII A'AGCT_T
Cuts at: 748 748
Size: 9730
HpaI GTT'AAC
Cuts at: 4162 7320 4162
Size: 3158 6572
KpnI G_GTAC'C
Cuts at: 2877 7055 2877
Size: 4178 5552
MfeI C'AATT_G
Cuts at: 7329 7329
Size: 9730
MluI A'CGCG_T
Cuts at: 9606 9606
Size: 9730
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3508 4699 5623 5892 6173 6273 8844 9028
Size: 2203 1191 924 269 281 100 2571 184
Cuts at: 9028 1305
Size: 2007
Fragments arranged by size:
2571 2203 2007 1191 924 281 269 184
100
MscI TGG'CCA
Cuts at: 10 64 1489 1832 4776 10
Size: 54 1425 343 2944 4964
Fragments arranged by size:
4964 2944 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2165 2285 2856 2885 5706 5844 5948
Size: 648 999 120 571 29 2821 138 104
Cuts at: 5948 6303 7688 8047 8206 518
Size: 355 1385 359 159 2042
Fragments arranged by size:
2821 2042 1385 999 648 571 359 355
159 138 120 104 29
MspA1I CmG'CkG
Cuts at: 1606 2942 5723 7146 7823 8971 9216 1606
Size: 1336 2781 1423 677 1148 245 2120
Fragments arranged by size:
2781 2120 1423 1336 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 8239
Fragments arranged by size:
8239 939 552
NdeI CA'TA_TG
Cuts at: 387 5290 387
Size: 4903 4827
NheI G'CTAG_C
Cuts at: 1052 2231 2600 1052
Size: 1179 369 8182
Fragments arranged by size:
8182 1179 369
NotI GC'GGCC_GC
Cuts at: 7177 7177
Size: 9730
NsiI A_TGCA'T
Cuts at: 3777 3777
Size: 9730
NspI r_CATG'y
Cuts at: 1132 1229 2597 3779 5843 7088 8633 1132
Size: 97 1368 1182 2064 1245 1545 2229
Fragments arranged by size:
2229 2064 1545 1368 1245 1182 97
PasI CC'CwG_GG
Cuts at: 1348 3124 1348
Size: 1776 7954
PciI A'CATG_T
Cuts at: 8629 8629
Size: 9730
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2056 4363 6995 7010 1108
Size: 948 2307 2632 15 3828
Fragments arranged by size:
3828 2632 2307 948 15
PmeI GTTT'AAAC
Cuts at: 7034 7034
Size: 9730
PmlI CAC'GTG
Cuts at: 5120 5120
Size: 9730
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 7799 7831 9312 9344 7799
Size: 32 1481 32 8185
Fragments arranged by size:
8185 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 3737 4174 4954 6935 3737
Size: 437 780 1981 6532
Fragments arranged by size:
6532 1981 780 437
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 9730
PsiI TTA'TAA
Cuts at: 7300 7300
Size: 9730
PstI C_TGCA'G
Cuts at: 830 3876 4044 4999 6061 6669 7082 830
Size: 3046 168 955 1062 608 413 3478
Fragments arranged by size:
3478 3046 1062 955 608 413 168
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 9730
PvuII CAG'CTG
Cuts at: 2942 5723 2942
Size: 2781 6949
SacI G_AGCT'C
Cuts at: 5941 5941
Size: 9730
SalI G'TCGA_C
Cuts at: 1936 4434 7072 1936
Size: 2498 2638 4594
Fragments arranged by size:
4594 2638 2498
SapI GCTCTTCn'nnn_
Cuts at: 3618 5889 3618
Size: 2271 7459
SbfI CC_TGCA'GG
Cuts at: 7082 7082
Size: 9730
ScaI AGT'ACT
Cuts at: 3254 5471 5752 6821 7978 9690 3254
Size: 2217 281 1069 1157 1712 3294
Fragments arranged by size:
3294 2217 1712 1157 1069 281
SexAI A'CCwGG_T
Cuts at: 4842 4920 4842
Size: 78 9652
SfcI C'TryA_G
Cuts at: 826 1045 2559 2715 3872 4040 4246 4443 4995
Size: 219 1514 156 1157 168 206 197 552
Cuts at: 4995 6057 6217 6665 6882 7078 8213 8894 9085
Size: 1062 160 448 217 196 1135 681 191
Cuts at: 9085 826
Size: 1471
Fragments arranged by size:
1514 1471 1157 1135 1062 681 552 448
219 217 206 197 196 191 168 160
156
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 9730
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 9730
SmaI CCC'GGG
Cuts at: 1872 7057 1872
Size: 5185 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2864 3758 3783 4292 5496 5930
Size: 197 926 921 894 25 509 1204 434
Cuts at: 5930 7834 8735 8997 820
Size: 1904 901 262 1553
Fragments arranged by size:
1904 1553 1204 926 921 901 894 509
434 262 197 25
SnaBI TAC'GTA
Cuts at: 493 3065 7521 493
Size: 2572 4456 2702
Fragments arranged by size:
4456 2702 2572
SpeI A'CTAG_T
Cuts at: 152 5441 152
Size: 5289 4441
SphI G_CATG'C
Cuts at: 1132 3779 7088 1132
Size: 2647 3309 3774
Fragments arranged by size:
3774 3309 2647
SspI AAT'ATT
Cuts at: 5 52 6037 7654 5
Size: 47 5985 1617 2081
Fragments arranged by size:
5985 2081 1617 47
StuI AGG'CCT
Cuts at: 1517 3316 1517
Size: 1799 7931
StyI C'CwwG_G
Cuts at: 513 1065 2004 4583 6042 6192 7169 513
Size: 552 939 2579 1459 150 977 3074
Fragments arranged by size:
3074 2579 1459 977 939 552 150
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3383 3947 5696 7757 7774 7927 8112
Size: 1255 1803 564 1749 2061 17 153 185
Cuts at: 8112 8557 325
Size: 445 1498
Fragments arranged by size:
2061 1803 1749 1498 1255 564 445 185
153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3252 3319 3689
Size: 274 80 33 51 1443 1275 67 370
Cuts at: 3689 4414 4927 5469 5655 5750 5836 6819 7976
Size: 725 513 542 186 95 86 983 1157
Cuts at: 7976 9688 96
Size: 1712 138
Fragments arranged by size:
1712 1443 1275 1157 983 725 542 513
370 274 186 138 95 86 80 67
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6559 6591 9690 9722 6559
Size: 32 3099 32 6567
Fragments arranged by size:
6567 3099 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 4352 1316
Size: 3036 6694
XbaI T'CTAG_A
Cuts at: 6026 7066 6026
Size: 1040 8690
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 6156 6965 6156
Size: 809 8921
XhoI C'TCGA_G
Cuts at: 1943 5496 1943
Size: 3553 6177
XmaI C'CCGG_G
Cuts at: 1870 7055 1870
Size: 5185 4545
XmnI GAAnn'nnTTC
Cuts at: 5424 5798 7859 5424
Size: 374 2061 7295
Fragments arranged by size:
7295 2061 374
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 7538 276
Size: 53 83 186 936 6004 2468
Fragments arranged by size:
6004 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BaeI BamHI BanI BanII BbsI BbvCI BcgI
BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NcoI NdeI NheI NotI NsiI NspI PasI
PciI PflMI PmeI PmlI PpiI PpuMI PshAI PsiI
PstI PvuI PvuII SacI SalI SapI SbfI ScaI
SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BbeI BplI
BsiWI BsmBI BstZ17I FseI FspAI KasI NaeI NarI
NgoMIV NruI PacI PfoI PspOMI PspXI PsrI RsrII
SacII SanDI SfiI SfoI SrfI SwaI