Restriction map for
pFN21AA0030
(Circular) (Six-base) MAPSORT of: pFN21AA0030.seq Check: 1407 from: 1 to: 7417
pFN21AA0030 7417 bp
With 182 enzymes: SgfI *
February 27, 2009 14:36 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2874 4522 2874
Size: 1648 5769
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4377 5227 278
Size: 53 83 186 936 2841 850 2468
Fragments arranged by size:
2841 2468 936 850 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2625 4760 1937
Size: 688 2135 4594
Fragments arranged by size:
4594 2135 688
Acc65I G'GTAC_C
Cuts at: 4738 4738
Size: 7417
AclI AA'CG_TT
Cuts at: 1563 5544 5917 1563
Size: 3981 373 3063
Fragments arranged by size:
3981 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 4184 4532 4645 5480 6864 7132 1632
Size: 2552 348 113 835 1384 268 1917
Fragments arranged by size:
2552 1917 1384 835 348 268 113
AfeI AGC'GCT
Cuts at: 4105 4105
Size: 7417
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 7220
AflIII A'CryG_T
Cuts at: 1203 2667 4128 6316 7293 1203
Size: 1464 1461 2188 977 1327
Fragments arranged by size:
2188 1464 1461 1327 977
AgeI A'CCGG_T
Cuts at: 6248 6248
Size: 7417
AhdI GACnn_n'nnGTC
Cuts at: 2325 6146 2325
Size: 3821 3596
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 7417
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 4364 1184
Size: 3180 4237
AlwNI CAG_nnn'CTG
Cuts at: 5160 6732 5160
Size: 1572 5845
ApaI G_GGCC'C
Cuts at: 2190 2576 3730 4564 2190
Size: 386 1154 834 5043
Fragments arranged by size:
5043 1154 834 386
ApaLI G'TGCA_C
Cuts at: 5474 6630 5474
Size: 1156 6261
ApoI r'AATT_y
Cuts at: 1493 3767 4726 4952 5104 7213 1493
Size: 2274 959 226 152 2109 1697
Fragments arranged by size:
2274 2109 1697 959 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5971 160
Size: 872 4939 1606
Fragments arranged by size:
4939 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7417
AvaI C'yCGr_G
Cuts at: 1870 1943 4742 1870
Size: 73 2799 4545
Fragments arranged by size:
4545 2799 73
AvrII C'CTAG_G
Cuts at: 2173 2173
Size: 7417
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 3112 3145 3112
Size: 33 7384
BamHI G'GATC_C
Cuts at: 4747 5114 4747
Size: 367 7050
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2755 3690 4738 6193 618
Size: 325 217 627 968 935 1048 1455 1842
Fragments arranged by size:
1842 1455 1048 968 935 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2172 2190 2220 2409 2576 3516 3730 3960
Size: 781 18 30 189 167 940 214 230
Cuts at: 3960 4116 4146 4564 4659 4736 1391
Size: 156 30 418 95 77 4072
Fragments arranged by size:
4072 940 781 418 230 214 189 167
156 95 77 30 30 18
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2638 3477 4374 928
Size: 971 739 839 897 3971
Fragments arranged by size:
3971 971 897 839 739
BbvCI CC'TCA_GC
Cuts at: 2894 2894
Size: 7417
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2605 2639 5608 5642 1903
Size: 34 668 34 2969 34 3678
Fragments arranged by size:
3678 2969 668 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3140 5309 6519 3140
Size: 2169 1210 4038
Fragments arranged by size:
4038 2169 1210
BclI T'GATC_A
Cuts at: 1548 3393 1548
Size: 1845 5572
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2276 6028 136
Size: 107 122 71 631 605 604 3752 1525
Fragments arranged by size:
3752 1525 631 605 604 122 107 71
BglII A'GATC_T
Cuts at: 2775 3205 3432 7412 2775
Size: 430 227 3980 2780
Fragments arranged by size:
3980 2780 430 227
BlpI GC'TnA_GC
Cuts at: 2969 4110 4834 2969
Size: 1141 724 5552
Fragments arranged by size:
5552 1141 724
Bme1580I G_kGCm'C
Cuts at: 1790 2190 2576 3730 4564 5478 6634 1790
Size: 400 386 1154 834 914 1156 2573
Fragments arranged by size:
2573 1156 1154 914 834 400 386
BmgBI CAC'GTC
Cuts at: 1318 2623 4054 1318
Size: 1305 1431 4681
Fragments arranged by size:
4681 1431 1305
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4004 5148 6106
Size: 416 53 83 399 24 2587 1144 958
Cuts at: 6106 442
Size: 1753
Fragments arranged by size:
2587 1753 1144 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7417
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2234 2905 3773 2234
Size: 671 868 5878
Fragments arranged by size:
5878 868 671
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2065 2107 2213 2546 2603 2816 4421 6077
Size: 826 42 106 333 57 213 1605 1656
Cuts at: 6077 1239
Size: 2579
Fragments arranged by size:
2579 1656 1605 826 333 213 106 57
42
Bpu10I CC'TnA_GC
Cuts at: 2894 2894
Size: 7417
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3587 5542 6407 6705 3587
Size: 1955 865 298 4299
Fragments arranged by size:
4299 1955 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3098 3567 6080 882
Size: 617 1599 469 2513 2219
Fragments arranged by size:
2513 2219 1599 617 469
BsaAI yAC'GTr
Cuts at: 493 1204 2731 3334 3545 4131 5208 493
Size: 711 1527 603 211 586 1077 2702
Fragments arranged by size:
2702 1527 1077 711 603 586 211
BsaBI GATnn'nnATC
Cuts at: 4622 7411 4622
Size: 2789 4628
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2268 2955 3189 3656
Size: 53 83 186 936 735 687 234 467
Cuts at: 3656 4374 5224 5606 6241 275
Size: 718 850 382 635 1451
Fragments arranged by size:
1451 936 850 735 718 687 635 467
382 234 186 83 53
BsaWI w'CCGG_w
Cuts at: 5850 6248 6522 6669 7080 5850
Size: 398 274 147 411 6187
Fragments arranged by size:
6187 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 7192
Fragments arranged by size:
7192 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2228 2327 2441 2450 2504 2513 2894 3116
Size: 1042 99 114 9 54 9 381 222
Cuts at: 3116 3188 3762 4094 4097 1186
Size: 72 574 332 3 4506
Fragments arranged by size:
4506 1042 574 381 332 222 114 99
72 54 9 9 3
BseYI C'CCAG_C
Cuts at: 2049 2964 3722 4067 4563 6620 7116 2049
Size: 915 758 345 496 2057 496 2350
Fragments arranged by size:
2350 2057 915 758 496 496 345
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2932 3859 1163
Size: 573 1196 927 4721
Fragments arranged by size:
4721 1196 927 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2830 3078 4867 5628 6656 1540
Size: 275 186 829 248 1789 761 1028 2301
Fragments arranged by size:
2301 1789 1028 829 761 275 248 186
BsiHKAI G_wGCw'C
Cuts at: 2220 2730 3564 4659 5478 5563 6634 2220
Size: 510 834 1095 819 85 1071 3003
Fragments arranged by size:
3003 1095 1071 834 819 510 85
BsmI GAATG_Cn'
Cuts at: 4928 5021 4928
Size: 93 7324
BsmBI CGTCTCn'nnnn_
Cuts at: 3225 3225
Size: 7417
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2172 2190 2220 2409 2576 2730 3516
Size: 399 382 18 30 189 167 154 786
Cuts at: 3516 3564 3730 3960 4116 4146 4564 4659 4736
Size: 48 166 230 156 30 418 95 77
Cuts at: 4736 5478 5563 6634 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1071 786 742 418 399 382 230
189 167 166 156 154 95 85 77
48 30 30 18
BspEI T'CCGG_A
Cuts at: 7080 7080
Size: 7417
BspHI T'CATG_A
Cuts at: 3466 5306 7036 3466
Size: 1840 1730 3847
Fragments arranged by size:
3847 1840 1730
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2874 4522 4772 844
Size: 2030 1648 250 3489
Fragments arranged by size:
3489 2030 1648 250
BsrBI CCG'CTC
Cuts at: 1394 1823 2347 2470 3180 5304 1394
Size: 429 524 123 710 2124 3507
Fragments arranged by size:
3507 2124 710 524 429 123
BsrDI GCAATG_nn'
Cuts at: 65 1248 2071 3444 3882 5912 6086 65
Size: 1183 823 1373 438 2030 174 1396
Fragments arranged by size:
2030 1396 1373 1183 823 438 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2758 3077 3168 6061 6248 1700
Size: 110 948 319 91 2893 187 2869
Fragments arranged by size:
2893 2869 948 319 187 110 91
BsrGI T'GTAC_A
Cuts at: 96 4242 96
Size: 4146 3271
BssHII G'CGCG_C
Cuts at: 1925 4337 1925
Size: 2412 5005
BssSI C'ACGA_G
Cuts at: 5477 6489 5477
Size: 1012 6405
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2997 794
Size: 2203 5214
BstBI TT'CG_AA
Cuts at: 6221 6221
Size: 7417
BstEII G'GTnAC_C
Cuts at: 1184 2938 1184
Size: 1754 5663
BstXI CCAn_nnnn'nTGG
Cuts at: 2935 3334 2935
Size: 399 7018
BstYI r'GATC_y
Cuts at: 1972 2530 2586 2775 3205 3432 4747 5114 5499
Size: 558 56 189 430 227 1315 367 385
Cuts at: 5499 5516 6968 7054 7066 7412 1972
Size: 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 1315 558 430 385 367 346
227 189 86 56 17 12
Bsu36I CC'TnA_GG
Cuts at: 2751 4253 2751
Size: 1502 5915
BtgI C'CryG_G
Cuts at: 513 1065 2004 2031 2401 2475 2632 3813 4002
Size: 552 939 27 370 74 157 1181 189
Cuts at: 4002 513
Size: 3928
Fragments arranged by size:
3928 1181 939 552 370 189 157 74
27
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2436 487
Size: 683 56 1210 5468
Fragments arranged by size:
5468 1210 683 56
BtsI GCAGTG_nn'
Cuts at: 3285 4933 5727 5747 3285
Size: 1648 794 20 4955
Fragments arranged by size:
4955 1648 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7417
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 3526 3561 529
Size: 35 2962 35 4385
Fragments arranged by size:
4385 2962 35 35
DraI TTT'AAA
Cuts at: 4721 4721
Size: 7417
DraIII CAC_nnn'GTG
Cuts at: 2548 2548
Size: 7417
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 6424 809
Size: 507 5108 1802
Fragments arranged by size:
5108 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2089 2131 2322
Size: 54 1425 50 275 18 259 42 191
Cuts at: 2322 2377 2635 2808 3075 3166 3336 3646 4092
Size: 55 258 173 267 91 170 310 446
Cuts at: 4092 4233 4383 4864 5753 7382 8
Size: 141 150 481 889 1629 43
Fragments arranged by size:
1629 1425 889 481 446 310 275 267
259 258 191 173 170 150 141 91
55 54 50 43 42 18
EagI C'GGCC_G
Cuts at: 1537 1812 3075 4864 1537
Size: 275 1263 1789 4090
Fragments arranged by size:
4090 1789 1263 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2196 4508 4664 5347 1353
Size: 843 2312 156 683 3423
Fragments arranged by size:
3423 2312 843 683 156
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2024 2777 4340 6004 6378 6524
Size: 1064 138 435 753 1563 1664 374 146
Cuts at: 6524 387
Size: 1280
Fragments arranged by size:
1664 1563 1280 1064 753 435 374 146
138
EcoICRI GAG'CTC
Cuts at: 2218 4657 2218
Size: 2439 4978
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2065 2107 2213 2546 2603 2816 4184
Size: 393 433 42 106 333 57 213 1368
Cuts at: 4184 4421 4532 4645 5480 6077 6864 7132 1239
Size: 237 111 113 835 597 787 268 1524
Fragments arranged by size:
1524 1368 835 787 597 433 393 333
268 237 213 113 111 106 57 42
EcoNI CCTnn'n_nnAGG
Cuts at: 2750 3407 4151 2750
Size: 657 744 6016
Fragments arranged by size:
6016 744 657
EcoO109I rG'GnC_Cy
Cuts at: 2186 2187 2383 2573 3014 3726 3727 4159 4560
Size: 1 196 190 441 712 1 432 401
Cuts at: 4560 4582 2186
Size: 22 5021
Fragments arranged by size:
5021 712 441 432 401 196 190 22
1 1
EcoRI G'AATT_C
Cuts at: 4726 5104 4726
Size: 378 7039
EcoRV GAT'ATC
Cuts at: 3553 3553
Size: 7417
FseI GG_CCGG'CC
Cuts at: 3081 3081
Size: 7417
FspI TGC'GCA
Cuts at: 2615 4439 4448 5923 2615
Size: 1824 9 1475 4109
Fragments arranged by size:
4109 1824 1475 9
HaeII r_GCGC'y
Cuts at: 1678 2443 2458 3913 4107 6564 1678
Size: 765 15 1455 194 2457 2531
Fragments arranged by size:
2531 2457 1455 765 194 15
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2144 2176 2311 2343 3056
Size: 32 135 32 572 32 135 32 713
Cuts at: 3056 3088 3097 3129 3417 3449 3605 3637 6058
Size: 32 9 32 288 32 156 32 2421
Cuts at: 6058 6090 6132 6164 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 2421 713 572 288 156 135 135
42 32 32 32 32 32 32 32
32 32 32 9
HincII GTy'rAC
Cuts at: 669 1657 1938 2626 3094 4378 4761 5007 669
Size: 988 281 688 468 1284 383 246 3079
Fragments arranged by size:
3079 1284 988 688 468 383 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 7417
HpaI GTT'AAC
Cuts at: 5007 5007
Size: 7417
KpnI G_GTAC'C
Cuts at: 4742 4742
Size: 7417
MfeI C'AATT_G
Cuts at: 5016 5016
Size: 7417
MluI A'CGCG_T
Cuts at: 7293 7293
Size: 7417
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 6531 6715 1305
Size: 5226 184 2007
Fragments arranged by size:
5226 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2810 3338 3648 4094 4235
Size: 54 1425 343 978 528 310 446 141
Cuts at: 4235 4385 10
Size: 150 3042
Fragments arranged by size:
3042 1425 978 528 446 343 310 150
141 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2847 4292 4391 5375 5734 5893 518
Size: 648 1681 1445 99 984 359 159 2042
Fragments arranged by size:
2042 1681 1445 984 648 359 159 99
MspA1I CmG'CkG
Cuts at: 1606 2902 2917 2946 3219 3688 4833 5510 6658
Size: 1296 15 29 273 469 1145 677 1148
Cuts at: 6658 6903 1606
Size: 245 2120
Fragments arranged by size:
2120 1296 1148 1145 677 469 273 245
29 15
NaeI GCC'GGC
Cuts at: 3079 3170 3079
Size: 91 7326
NcoI C'CATG_G
Cuts at: 513 1065 2004 2031 3813 513
Size: 552 939 27 1782 4117
Fragments arranged by size:
4117 1782 939 552 27
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7417
NgoMIV G'CCGG_C
Cuts at: 3077 3168 3077
Size: 91 7326
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7417
NotI GC'GGCC_GC
Cuts at: 4864 4864
Size: 7417
NspI r_CATG'y
Cuts at: 1132 1229 2398 2671 4447 4775 6320 1132
Size: 97 1169 273 1776 328 1545 2229
Fragments arranged by size:
2229 1776 1545 1169 328 273 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 7417
PciI A'CATG_T
Cuts at: 2667 6316 2667
Size: 3649 3768
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 7417
PfoI T'CCnGG_A
Cuts at: 3138 4014 3138
Size: 876 6541
PmeI GTTT'AAAC
Cuts at: 4721 4721
Size: 7417
PmlI CAC'GTG
Cuts at: 2731 3334 2731
Size: 603 6814
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5486 5518 6999 7031 5486
Size: 32 1481 32 5872
Fragments arranged by size:
5872 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 3014 4159 4582 3014
Size: 1145 423 5849
Fragments arranged by size:
5849 1145 423
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7417
PsiI TTA'TAA
Cuts at: 4987 4987
Size: 7417
PspOMI G'GGCC_C
Cuts at: 2186 2572 3726 4560 2186
Size: 386 1154 834 5043
Fragments arranged by size:
5043 1154 834 386
PstI C_TGCA'G
Cuts at: 830 2775 3867 4710 4769 830
Size: 1945 1092 843 59 3478
Fragments arranged by size:
3478 1945 1092 843 59
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7417
PvuII CAG'CTG
Cuts at: 2902 2917 2946 3219 2902
Size: 15 29 273 7100
Fragments arranged by size:
7100 273 29 15
SacI G_AGCT'C
Cuts at: 2220 4659 2220
Size: 2439 4978
SalI G'TCGA_C
Cuts at: 1936 2624 4759 1936
Size: 688 2135 4594
Fragments arranged by size:
4594 2135 688
SapI GCTCTTCn'nnn_
Cuts at: 4508 4664 4508
Size: 156 7261
SbfI CC_TGCA'GG
Cuts at: 3867 4769 3867
Size: 902 6515
ScaI AGT'ACT
Cuts at: 5665 7377 5665
Size: 1712 5705
SfcI C'TryA_G
Cuts at: 826 1045 2771 3360 3863 4706 4765 5900 6581
Size: 219 1726 589 503 843 59 1135 681
Cuts at: 6581 6772 826
Size: 191 1471
Fragments arranged by size:
1726 1471 1135 843 681 589 503 219
191 59
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7417
SgrAI Cr'CCGG_yG
Cuts at: 1700 2758 1700
Size: 1058 6359
SmaI CCC'GGG
Cuts at: 1872 4744 1872
Size: 2872 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3602 5521 6422 6684 820
Size: 197 926 1659 1919 901 262 1553
Fragments arranged by size:
1919 1659 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 5208 493
Size: 4715 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7417
SphI G_CATG'C
Cuts at: 1132 2398 4447 4775 1132
Size: 1266 2049 328 3774
Fragments arranged by size:
3774 2049 1266 328
SspI AAT'ATT
Cuts at: 5 52 5341 5
Size: 47 5289 2081
Fragments arranged by size:
5289 2081 47
StuI AGG'CCT
Cuts at: 1517 3486 1517
Size: 1969 5448
StyI C'CwwG_G
Cuts at: 513 1065 2004 2031 2173 3183 3408 3714 3813
Size: 552 939 27 142 1010 225 306 99
Cuts at: 3813 4185 4856 513
Size: 372 671 3074
Fragments arranged by size:
3074 1010 939 671 552 372 306 225
142 99 27
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2548 3389 3759 5444 5461 5614 5799
Size: 1255 968 841 370 1685 17 153 185
Cuts at: 5799 6244 325
Size: 445 1498
Fragments arranged by size:
1685 1498 1255 968 841 445 370 185
153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2982 4170 4242
Size: 274 80 33 51 1443 1005 1188 72
Cuts at: 4242 5663 7375 96
Size: 1421 1712 138
Fragments arranged by size:
1712 1443 1421 1188 1005 274 138 80
72 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4292 4324 7377 7409 4292
Size: 32 3053 32 4300
Fragments arranged by size:
4300 3053 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 4010 4373 1316
Size: 2694 363 4360
Fragments arranged by size:
4360 2694 363
XbaI T'CTAG_A
Cuts at: 4753 4753
Size: 7417
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2260 4318 2260
Size: 2058 5359
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7417
XmaI C'CCGG_G
Cuts at: 1870 4742 1870
Size: 2872 4545
XmnI GAAnn'nnTTC
Cuts at: 4673 5546 4673
Size: 873 6544
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4375 5225 276
Size: 53 83 186 936 2841 850 2468
Fragments arranged by size:
2841 2468 936 850 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI AvrII BaeI BamHI BanI
BanII BbsI BbvCI BcgI BciVI BclI BglI BglII
BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI
BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV
FseI FspI HaeII Hin4I HincII HindIII HpaI KpnI
MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI
NdeI NgoMIV NheI NotI NspI PasI PciI PflMI
PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI
PstI PvuI PvuII SacI SalI SapI SbfI ScaI
SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AloI AscI BbeI BfrBI BsiWI BstZ17I FalI FspAI
KasI NarI NruI NsiI PacI PspXI PsrI RsrII
SacII SanDI SexAI SfiI SfoI SrfI SwaI