Restriction map for
pFN21AA0018
(Circular) (Six-base) MAPSORT of: pFN21AA0018.seq Check: 5454 from: 1 to: 6253
pFN21AA0018 6253 bp
With 182 enzymes: SgfI *
January 16, 2009 15:22 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2224 2224
Size: 6253
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4063 278
Size: 53 83 186 936 2527 2468
Fragments arranged by size:
2527 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3225 3596 1937
Size: 1288 371 4594
Fragments arranged by size:
4594 1288 371
Acc65I G'GTAC_C
Cuts at: 3574 3574
Size: 6253
AclI AA'CG_TT
Cuts at: 1563 4380 4753 1563
Size: 2817 373 3063
Fragments arranged by size:
3063 2817 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2084 2960 3122 4316 5700 5968 1632
Size: 452 876 162 1194 1384 268 1917
Fragments arranged by size:
1917 1384 1194 876 452 268 162
AfeI AGC'GCT
Cuts at: 2096 2191 2096
Size: 95 6158
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6056
AflIII A'CryG_T
Cuts at: 1203 2311 5152 6129 1203
Size: 1108 2841 977 1327
Fragments arranged by size:
2841 1327 1108 977
AgeI A'CCGG_T
Cuts at: 5084 5084
Size: 6253
AhdI GACnn_n'nnGTC
Cuts at: 4982 4982
Size: 6253
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6253
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2618 1184
Size: 1434 4819
AlwNI CAG_nnn'CTG
Cuts at: 3196 3360 3412 3996 5568 3196
Size: 164 52 584 1572 3881
Fragments arranged by size:
3881 1572 584 164 52
ApaLI G'TGCA_C
Cuts at: 2275 3275 4310 5466 2275
Size: 1000 1035 1156 3062
Fragments arranged by size:
3062 1156 1035 1000
ApoI r'AATT_y
Cuts at: 1493 3562 3788 3940 6049 1493
Size: 2069 226 152 2109 1697
Fragments arranged by size:
2109 2069 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4807 160
Size: 872 3775 1606
Fragments arranged by size:
3775 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6253
AvaI C'yCGr_G
Cuts at: 1870 1943 3515 3578 1870
Size: 73 1572 63 4545
Fragments arranged by size:
4545 1572 73 63
BamHI G'GATC_C
Cuts at: 3583 3950 3583
Size: 367 5886
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2283 3079 3169 3574 5029
Size: 325 217 627 496 796 90 405 1455
Cuts at: 5029 618
Size: 1842
Fragments arranged by size:
1842 1455 796 627 496 405 325 217
90
BanII G_rGCy'C
Cuts at: 1391 2013 2409 2874 3021 3303 3572 1391
Size: 622 396 465 147 282 269 4072
Fragments arranged by size:
4072 622 465 396 282 269 147
BbeI G_GCGC'C
Cuts at: 2287 2287
Size: 6253
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2336 2948 928
Size: 971 437 612 4233
Fragments arranged by size:
4233 971 612 437
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2503 2537 4444 4478 1903
Size: 34 566 34 1907 34 3678
Fragments arranged by size:
3678 1907 566 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4145 5355 4145
Size: 1210 5043
BclI T'GATC_A
Cuts at: 1548 2349 2505 1548
Size: 801 156 5296
Fragments arranged by size:
5296 801 156
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3267 4864 136
Size: 107 122 71 631 605 1595 1597 1525
Fragments arranged by size:
1597 1595 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 3090 3531 6248 3090
Size: 441 2717 3095
Fragments arranged by size:
3095 2717 441
BlpI GC'TnA_GC
Cuts at: 2183 3670 2183
Size: 1487 4766
Bme1580I G_kGCm'C
Cuts at: 1790 2279 2516 3174 3279 4314 5470 1790
Size: 489 237 658 105 1035 1156 2573
Fragments arranged by size:
2573 1156 1035 658 489 237 105
BmgBI CAC'GTC
Cuts at: 1318 3223 1318
Size: 1905 4348
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3984 4942 442
Size: 416 53 83 399 24 2567 958 1753
Fragments arranged by size:
2567 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6253
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2093 2768 2981 3005 3395 4913 1239
Size: 854 675 213 24 390 1518 2579
Fragments arranged by size:
2579 1518 854 675 390 213 24
Bpu10I CC'TnA_GC
Cuts at: 2475 2475
Size: 6253
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2995 4378 5243 5541 2995
Size: 1383 865 298 3707
Fragments arranged by size:
3707 1383 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3113 4916 882
Size: 617 1614 1803 2219
Fragments arranged by size:
2219 1803 1614 617
BsaAI yAC'GTr
Cuts at: 493 1204 2158 2312 3151 4044 493
Size: 711 954 154 839 893 2702
Fragments arranged by size:
2702 954 893 839 711 154
BsaBI GATnn'nnATC
Cuts at: 2693 6247 2693
Size: 3554 2699
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2284 3507 4060 4442
Size: 53 83 186 936 751 1223 553 382
Cuts at: 4442 5077 275
Size: 635 1451
Fragments arranged by size:
1451 1223 936 751 635 553 382 186
83 53
BsaWI w'CCGG_w
Cuts at: 4686 5084 5358 5505 5916 4686
Size: 398 274 147 411 5023
Fragments arranged by size:
5023 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 6028
Fragments arranged by size:
6028 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3455 1186
Size: 2269 3984
BseYI C'CCAG_C
Cuts at: 2097 2665 2781 2873 3257 3493 5456 5952 2097
Size: 568 116 92 384 236 1963 496 2398
Fragments arranged by size:
2398 1963 568 496 384 236 116 92
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2186 2548 3176 3182 1163
Size: 573 450 362 628 6 4234
Fragments arranged by size:
4234 628 573 450 362 6
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3703 4464 5492 1540
Size: 275 186 1702 761 1028 2301
Fragments arranged by size:
2301 1702 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2085 2279 3021 3279 3303 4314 4399 5470 2085
Size: 194 742 258 24 1011 85 1071 2868
Fragments arranged by size:
2868 1071 1011 742 258 194 85 24
BsmI GAATG_Cn'
Cuts at: 3764 3857 3764
Size: 93 6160
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2013 2085 2279 2409 2516 2874 3021
Size: 399 223 72 194 130 107 358 147
Cuts at: 3021 3174 3279 3303 3572 4314 4399 5470 1391
Size: 153 105 24 269 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 358 269 223 194
153 147 130 107 105 85 72 24
BspEI T'CCGG_A
Cuts at: 5916 5916
Size: 6253
BspHI T'CATG_A
Cuts at: 2346 2853 4142 5872 2346
Size: 507 1289 1730 2727
Fragments arranged by size:
2727 1730 1289 507
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2224 3608 844
Size: 1380 1384 3489
Fragments arranged by size:
3489 1384 1380
BsrBI CCG'CTC
Cuts at: 1394 1823 2128 4140 1394
Size: 429 305 2012 3507
Fragments arranged by size:
3507 2012 429 305
BsrDI GCAATG_nn'
Cuts at: 65 1248 4748 4922 65
Size: 1183 3500 174 1396
Fragments arranged by size:
3500 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4897 5084 1700
Size: 110 3087 187 2869
Fragments arranged by size:
3087 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6253
BssHII G'CGCG_C
Cuts at: 1925 2034 2161 1925
Size: 109 127 6017
Fragments arranged by size:
6017 127 109
BssSI C'ACGA_G
Cuts at: 2204 2774 4313 5325 2204
Size: 570 1539 1012 3132
Fragments arranged by size:
3132 1539 1012 570
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2215 3163 3196 794
Size: 1421 948 33 3851
Fragments arranged by size:
3851 1421 948 33
BstBI TT'CG_AA
Cuts at: 5057 5057
Size: 6253
BstEII G'GTnAC_C
Cuts at: 1184 2413 1184
Size: 1229 5024
BstYI r'GATC_y
Cuts at: 1972 2688 3090 3531 3583 3950 4335 4352 5804
Size: 716 402 441 52 367 385 17 1452
Cuts at: 5804 5890 5902 6248 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 716 441 402 385 367 346
86 52 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2418 2909 513
Size: 552 939 414 491 3857
Fragments arranged by size:
3857 939 552 491 414
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2620 487
Size: 683 56 1394 4120
Fragments arranged by size:
4120 1394 683 56
BtsI GCAGTG_nn'
Cuts at: 2565 3769 4563 4583 2565
Size: 1204 794 20 4235
Fragments arranged by size:
4235 1204 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6253
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6218
DraI TTT'AAA
Cuts at: 3557 3557
Size: 6253
DraIII CAC_nnn'GTG
Cuts at: 2312 2803 3151 3154 2312
Size: 491 348 3 5411
Fragments arranged by size:
5411 491 348 3
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 3221 5260 809
Size: 507 1905 2039 1802
Fragments arranged by size:
2039 1905 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2025 2679 3700
Size: 54 1425 50 275 18 195 654 1021
Cuts at: 3700 4589 6218 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 1021 889 654 275 195 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3700 1537
Size: 275 1888 4090
Fragments arranged by size:
4090 1888 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2048 2120 4183 1353
Size: 695 72 2063 3423
Fragments arranged by size:
3423 2063 695 72
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4840 5214 5360 387
Size: 1064 138 3251 374 146 1280
Fragments arranged by size:
3251 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 3019 3301 3019
Size: 282 5971
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2084 2093 2768 2960 2981 3005 3122
Size: 393 452 9 675 192 21 24 117
Cuts at: 3122 3395 4316 4913 5700 5968 1239
Size: 273 921 597 787 268 1524
Fragments arranged by size:
1524 921 787 675 597 452 393 273
268 192 117 24 21 9
EcoNI CCTnn'n_nnAGG
Cuts at: 2828 3188 3364 2828
Size: 360 176 5717
Fragments arranged by size:
5717 360 176
EcoO109I rG'GnC_Cy
Cuts at: 2743 2956 3193 3357 2743
Size: 213 237 164 5639
Fragments arranged by size:
5639 237 213 164
EcoRI G'AATT_C
Cuts at: 3562 3940 3562
Size: 378 5875
EcoRV GAT'ATC
Cuts at: 2146 2146
Size: 6253
FspI TGC'GCA
Cuts at: 3128 4759 3128
Size: 1631 4622
HaeII r_GCGC'y
Cuts at: 1678 2098 2193 2287 5400 1678
Size: 420 95 94 3113 2531
Fragments arranged by size:
3113 2531 420 95 94
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2516 2548 3067 3099 4894
Size: 32 135 32 944 32 519 32 1795
Cuts at: 4894 4926 4968 5000 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1795 944 519 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3597 3843 669
Size: 988 281 1659 246 3079
Fragments arranged by size:
3079 1659 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6253
HpaI GTT'AAC
Cuts at: 3843 3843
Size: 6253
KasI G'GCGC_C
Cuts at: 2283 2283
Size: 6253
KpnI G_GTAC'C
Cuts at: 3578 3578
Size: 6253
MfeI C'AATT_G
Cuts at: 3852 3852
Size: 6253
MluI A'CGCG_T
Cuts at: 6129 6129
Size: 6253
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2578 5367 5551 1305
Size: 1273 2789 184 2007
Fragments arranged by size:
2789 2007 1273 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4431
Fragments arranged by size:
4431 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3167 4211 4570 4729 518
Size: 648 2001 1044 359 159 2042
Fragments arranged by size:
2042 2001 1044 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2684 2785 3373 3669 4346 5494 5739 1606
Size: 1078 101 588 296 677 1148 245 2120
Fragments arranged by size:
2120 1148 1078 677 588 296 245 101
NarI GG'CG_CC
Cuts at: 2284 2284
Size: 6253
NcoI C'CATG_G
Cuts at: 513 1065 2004 2418 513
Size: 552 939 414 4348
Fragments arranged by size:
4348 939 552 414
NdeI CA'TA_TG
Cuts at: 387 3322 387
Size: 2935 3318
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6253
NotI GC'GGCC_GC
Cuts at: 3700 3700
Size: 6253
NspI r_CATG'y
Cuts at: 1132 1229 2927 3166 3611 5156 1132
Size: 97 1698 239 445 1545 2229
Fragments arranged by size:
2229 1698 1545 445 239 97
PasI CC'CwG_GG
Cuts at: 1348 3112 1348
Size: 1764 4489
PciI A'CATG_T
Cuts at: 5152 5152
Size: 6253
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2454 2803 1108
Size: 1346 349 4558
Fragments arranged by size:
4558 1346 349
PfoI T'CCnGG_A
Cuts at: 3021 3021
Size: 6253
PmeI GTTT'AAAC
Cuts at: 3557 3557
Size: 6253
PmlI CAC'GTG
Cuts at: 3151 3151
Size: 6253
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4322 4354 5835 5867 4322
Size: 32 1481 32 4708
Fragments arranged by size:
4708 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 3357 3357
Size: 6253
PshAI GACnn'nnGTC
Cuts at: 1613 2849 1613
Size: 1236 5017
PsiI TTA'TAA
Cuts at: 3823 3823
Size: 6253
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 2534 2566 2534
Size: 32 6221
PstI C_TGCA'G
Cuts at: 830 3189 3605 830
Size: 2359 416 3478
Fragments arranged by size:
3478 2359 416
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6253
PvuII CAG'CTG
Cuts at: 3373 3373
Size: 6253
SacI G_AGCT'C
Cuts at: 3021 3303 3021
Size: 282 5971
SalI G'TCGA_C
Cuts at: 1936 3595 1936
Size: 1659 4594
SapI GCTCTTCn'nnn_
Cuts at: 2048 2048
Size: 6253
SbfI CC_TGCA'GG
Cuts at: 3605 3605
Size: 6253
ScaI AGT'ACT
Cuts at: 4501 6213 4501
Size: 1712 4541
SfcI C'TryA_G
Cuts at: 826 1045 3185 3601 4736 5417 5608 826
Size: 219 2140 416 1135 681 191 1471
Fragments arranged by size:
2140 1471 1135 681 416 219 191
SfoI GGC'GCC
Cuts at: 2285 2285
Size: 6253
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6253
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6253
SmaI CCC'GGG
Cuts at: 1872 3580 1872
Size: 1708 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2974 4357 5258 5520 820
Size: 197 926 1031 1383 901 262 1553
Fragments arranged by size:
1553 1383 1031 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4044 493
Size: 3551 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6253
SphI G_CATG'C
Cuts at: 1132 3611 1132
Size: 2479 3774
SspI AAT'ATT
Cuts at: 5 52 4177 5
Size: 47 4125 2081
Fragments arranged by size:
4125 2081 47
StuI AGG'CCT
Cuts at: 1517 3270 1517
Size: 1753 4500
StyI C'CwwG_G
Cuts at: 513 1065 2004 2409 2418 3692 513
Size: 552 939 405 9 1274 3074
Fragments arranged by size:
3074 1274 939 552 405 9
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2044 2089 2158 2786 4280 4297 4450
Size: 1255 464 45 69 628 1494 17 153
Cuts at: 4450 4635 5080 325
Size: 185 445 1498
Fragments arranged by size:
1498 1494 1255 628 464 445 185 153
69 45 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2322 2964 4499
Size: 274 80 33 51 1443 345 642 1535
Cuts at: 4499 6211 96
Size: 1712 138
Fragments arranged by size:
1712 1535 1443 642 345 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6213 6245 6213
Size: 32 6221
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6253
XbaI T'CTAG_A
Cuts at: 3589 3589
Size: 6253
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6253
XmaI C'CCGG_G
Cuts at: 1870 3578 1870
Size: 1708 4545
XmnI GAAnn'nnTTC
Cuts at: 2798 4382 2798
Size: 1584 4669
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4061 276
Size: 53 83 186 936 2527 2468
Fragments arranged by size:
2527 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
EcoRV FspI HaeII Hin4I HincII HindIII HpaI KasI
KpnI MfeI MluI MmeI MscI MslI MspA1I NarI
NcoI NdeI NheI NotI NspI PasI PciI PflMI
PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PsrI
PstI PvuI PvuII SacI SalI SapI SbfI ScaI
SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AloI ApaI AscI AvrII BaeI BbvCI BfrBI BplI
BsiWI BsmBI BstXI BstZ17I Bsu36I FalI FseI FspAI
NaeI NgoMIV NruI NsiI PacI PspOMI PspXI RsrII
SacII SanDI SexAI SfiI SrfI SwaI XcmI