Restriction map for
pFN21AA0013
(Circular) (Six-base) MAPSORT of: pFN21AA0013.seq Check: 8904 from: 1 to: 7774
pFN21AA0013 7774 bp
With 182 enzymes: SgfI *
January 16, 2009 15:22 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3182 3691 3182
Size: 509 7265
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5584 278
Size: 53 83 186 936 4048 2468
Fragments arranged by size:
4048 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4835 5117 1937
Size: 2898 282 4594
Fragments arranged by size:
4594 2898 282
Acc65I G'GTAC_C
Cuts at: 5095 5095
Size: 7774
AclI AA'CG_TT
Cuts at: 1563 5901 6274 1563
Size: 4338 373 3063
Fragments arranged by size:
4338 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2600 5837 7221 7489 1632
Size: 968 3237 1384 268 1917
Fragments arranged by size:
3237 1917 1384 968 268
AflII C'TTAA_G
Cuts at: 820 1017 2366 4496 820
Size: 197 1349 2130 4098
Fragments arranged by size:
4098 2130 1349 197
AflIII A'CryG_T
Cuts at: 1203 4307 4373 6673 7650 1203
Size: 3104 66 2300 977 1327
Fragments arranged by size:
3104 2300 1327 977 66
AgeI A'CCGG_T
Cuts at: 3100 6605 3100
Size: 3505 4269
AhdI GACnn_n'nnGTC
Cuts at: 6503 6503
Size: 7774
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 7774
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 7774
AlwNI CAG_nnn'CTG
Cuts at: 2020 2047 2276 4391 5517 7089 2020
Size: 27 229 2115 1126 1572 2705
Fragments arranged by size:
2705 2115 1572 1126 229 27
ApaLI G'TGCA_C
Cuts at: 5831 6987 5831
Size: 1156 6618
ApoI r'AATT_y
Cuts at: 1493 2891 3317 4151 5083 5309 5461 7570 1493
Size: 1398 426 834 932 226 152 2109 1697
Fragments arranged by size:
2109 1697 1398 932 834 426 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4539 6328 160
Size: 872 3507 1789 1606
Fragments arranged by size:
3507 1789 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7774
AvaI C'yCGr_G
Cuts at: 1870 1943 5099 1870
Size: 73 3156 4545
Fragments arranged by size:
4545 3156 73
AvrII C'CTAG_G
Cuts at: 4334 4334
Size: 7774
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 3087 3120 4996 5029 3087
Size: 33 1876 33 5832
Fragments arranged by size:
5832 1876 33 33
BamHI G'GATC_C
Cuts at: 5104 5471 5104
Size: 367 7407
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 5095 6550 618
Size: 325 217 627 3308 1455 1842
Fragments arranged by size:
3308 1842 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2397 5093 1391
Size: 1006 2696 4072
Fragments arranged by size:
4072 2696 1006
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2241 2480 2637 2857 928
Size: 971 342 239 157 220 5845
Fragments arranged by size:
5845 971 342 239 220 157
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2078 2112 5965 5999 1903
Size: 34 141 34 3853 34 3678
Fragments arranged by size:
3853 3678 141 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 5666 6876 5666
Size: 1210 6564
BclI T'GATC_A
Cuts at: 1548 3183 4429 1548
Size: 1635 1246 4893
Fragments arranged by size:
4893 1635 1246
BfrBI ATG'CAT
Cuts at: 2708 2708
Size: 7774
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 6385 136
Size: 107 122 71 631 605 4713 1525
Fragments arranged by size:
4713 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 3302 4857 7769 3302
Size: 1555 2912 3307
Fragments arranged by size:
3307 2912 1555
BlpI GC'TnA_GC
Cuts at: 2982 5191 2982
Size: 2209 5565
Bme1580I G_kGCm'C
Cuts at: 1790 5835 6991 1790
Size: 4045 1156 2573
Fragments arranged by size:
4045 2573 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 7774
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 5505 6463 442
Size: 416 53 83 399 24 4088 958 1753
Fragments arranged by size:
4088 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7774
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4792 4792
Size: 7774
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3513 4141 6434 1239
Size: 2274 628 2293 2579
Fragments arranged by size:
2579 2293 2274 628
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2910 3004 3656 5899 6764 7062 2910
Size: 94 652 2243 865 298 3622
Fragments arranged by size:
3622 2243 865 652 298 94
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2780 4972 6437 882
Size: 617 1281 2192 1465 2219
Fragments arranged by size:
2219 2192 1465 1281 617
BsaAI yAC'GTr
Cuts at: 493 1204 5565 493
Size: 711 4361 2702
Fragments arranged by size:
4361 2702 711
BsaBI GATnn'nnATC
Cuts at: 7768 7768
Size: 7774
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 5581 5963 6598 275
Size: 53 83 186 936 4048 382 635 1451
Fragments arranged by size:
4048 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 3100 3555 6207 6605 6879 7026 7437 3100
Size: 455 2652 398 274 147 411 3437
Fragments arranged by size:
3437 2652 455 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2856 2886 4591 4621 1178
Size: 30 165 30 1453 30 1705 30 4331
Fragments arranged by size:
4331 1705 1453 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 7774
BseYI C'CCAG_C
Cuts at: 2404 2943 6977 7473 2404
Size: 539 4034 496 2705
Fragments arranged by size:
4034 2705 539 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2322 4278 1163
Size: 573 586 1956 4659
Fragments arranged by size:
4659 1956 586 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2098 5224 5985 7013 1540
Size: 275 186 97 3126 761 1028 2301
Fragments arranged by size:
3126 2301 1028 761 275 186 97
BsiHKAI G_wGCw'C
Cuts at: 2884 4642 5835 5920 6991 2884
Size: 1758 1193 85 1071 3667
Fragments arranged by size:
3667 1758 1193 1071 85
BsmI GAATG_Cn'
Cuts at: 4825 5285 5378 4825
Size: 460 93 7221
Fragments arranged by size:
7221 460 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2397 2884 4642 5093 5835 5920 6991
Size: 399 607 487 1758 451 742 85 1071
Cuts at: 6991 1391
Size: 2174
Fragments arranged by size:
2174 1758 1071 742 607 487 451 399
85
BspEI T'CCGG_A
Cuts at: 7437 7437
Size: 7774
BspHI T'CATG_A
Cuts at: 4906 5663 7393 4906
Size: 757 1730 5287
Fragments arranged by size:
5287 1730 757
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3182 3691 5129 844
Size: 2338 509 1438 3489
Fragments arranged by size:
3489 2338 1438 509
BsrBI CCG'CTC
Cuts at: 1394 1823 5661 1394
Size: 429 3838 3507
Fragments arranged by size:
3838 3507 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2990 6269 6443 65
Size: 1183 1742 3279 174 1396
Fragments arranged by size:
3279 1742 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3100 6418 6605 1700
Size: 110 1290 3318 187 2869
Fragments arranged by size:
3318 2869 1290 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 7774
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7774
BssSI C'ACGA_G
Cuts at: 5834 6846 5834
Size: 1012 6762
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2047 794
Size: 1253 6521
BstBI TT'CG_AA
Cuts at: 3298 6578 3298
Size: 3280 4494
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 7774
BstYI r'GATC_y
Cuts at: 1972 2273 2555 3302 4283 4857 5104 5471 5856
Size: 301 282 747 981 574 247 367 385
Cuts at: 5856 5873 7325 7411 7423 7769 1972
Size: 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 981 747 574 385 367 346
301 282 247 86 17 12
Bsu36I CC'TnA_GG
Cuts at: 4973 4973
Size: 7774
BtgI C'CryG_G
Cuts at: 513 1065 2083 2121 513
Size: 552 1018 38 6166
Fragments arranged by size:
6166 1018 552 38
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 7035
Fragments arranged by size:
7035 683 56
BtsI GCAGTG_nn'
Cuts at: 2096 2162 3186 5290 6084 6104 2096
Size: 66 1024 2104 794 20 3766
Fragments arranged by size:
3766 2104 1024 794 66 20
ClaI AT'CG_AT
Cuts at: 1554 2700 3117 1554
Size: 1146 417 6211
Fragments arranged by size:
6211 1146 417
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 7739
DraI TTT'AAA
Cuts at: 2896 3414 3717 4461 5078 2896
Size: 518 303 744 617 5592
Fragments arranged by size:
5592 744 617 518 303
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 6781 809
Size: 507 5465 1802
Fragments arranged by size:
5465 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 4870 5221 6110
Size: 54 1425 50 275 18 3040 351 889
Cuts at: 6110 7739 8
Size: 1629 43
Fragments arranged by size:
3040 1629 1425 889 351 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 5221 1537
Size: 275 3409 4090
Fragments arranged by size:
4090 3409 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3086 4725 5704 1353
Size: 1733 1639 979 3423
Fragments arranged by size:
3423 1733 1639 979
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 6361 6735 6881 387
Size: 1064 138 4772 374 146 1280
Fragments arranged by size:
4772 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2600 3513 4141 5837 6434 7221 7489
Size: 393 968 913 628 1696 597 787 268
Cuts at: 7489 1239
Size: 1524
Fragments arranged by size:
1696 1524 968 913 787 628 597 393
268
EcoO109I rG'GnC_Cy
Cuts at: 3572 4700 4969 4998 3572
Size: 1128 269 29 6348
Fragments arranged by size:
6348 1128 269 29
EcoRI G'AATT_C
Cuts at: 4151 5083 5461 4151
Size: 932 378 6464
Fragments arranged by size:
6464 932 378
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 4903 4903
Size: 7774
FspI TGC'GCA
Cuts at: 6280 6280
Size: 7774
HaeII r_GCGC'y
Cuts at: 1678 6921 1678
Size: 5243 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2693 2725 3110 3142 4268
Size: 32 135 32 1121 32 385 32 1126
Cuts at: 4268 4300 6415 6447 6489 6521 1373
Size: 32 2115 32 42 32 2626
Fragments arranged by size:
2626 2115 1126 1121 385 135 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 5118 5364 669
Size: 988 281 3180 246 3079
Fragments arranged by size:
3180 3079 988 281 246
HindIII A'AGCT_T
Cuts at: 748 2203 3612 4451 748
Size: 1455 1409 839 4071
Fragments arranged by size:
4071 1455 1409 839
HpaI GTT'AAC
Cuts at: 5364 5364
Size: 7774
KpnI G_GTAC'C
Cuts at: 5099 5099
Size: 7774
MfeI C'AATT_G
Cuts at: 5373 5373
Size: 7774
MluI A'CGCG_T
Cuts at: 7650 7650
Size: 7774
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3523 4530 6888 7072 1305
Size: 2218 1007 2358 184 2007
Fragments arranged by size:
2358 2218 2007 1007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 4872 10
Size: 54 1425 343 3040 2912
Fragments arranged by size:
3040 2912 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 5732 6091 6250 518
Size: 648 4566 359 159 2042
Fragments arranged by size:
4566 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2408 5190 5867 7015 7260 1606
Size: 802 2782 677 1148 245 2120
Fragments arranged by size:
2782 2120 1148 802 677 245
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 7222
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7774
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7774
NotI GC'GGCC_GC
Cuts at: 5221 5221
Size: 7774
NsiI A_TGCA'T
Cuts at: 2710 2710
Size: 7774
NspI r_CATG'y
Cuts at: 1132 1229 4311 4377 5132 6677 1132
Size: 97 3082 66 755 1545 2229
Fragments arranged by size:
3082 2229 1545 755 97 66
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 7774
PciI A'CATG_T
Cuts at: 4307 4373 6673 4307
Size: 66 2300 5408
Fragments arranged by size:
5408 2300 66
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2189 1108
Size: 1081 6693
PfoI T'CCnGG_A
Cuts at: 2739 2739
Size: 7774
PmeI GTTT'AAAC
Cuts at: 5078 5078
Size: 7774
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5843 5875 7356 7388 5843
Size: 32 1481 32 6229
Fragments arranged by size:
6229 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 3572 4700 4969 3572
Size: 1128 269 6377
Fragments arranged by size:
6377 1128 269
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7774
PsiI TTA'TAA
Cuts at: 4848 5344 4848
Size: 496 7278
PstI C_TGCA'G
Cuts at: 830 2683 3193 5126 830
Size: 1853 510 1933 3478
Fragments arranged by size:
3478 1933 1853 510
PvuI CG_AT'CG
Cuts at: 2001 2098 2001
Size: 97 7677
PvuII CAG'CTG
Cuts at: 2408 2408
Size: 7774
SalI G'TCGA_C
Cuts at: 1936 5116 1936
Size: 3180 4594
SbfI CC_TGCA'GG
Cuts at: 5126 5126
Size: 7774
ScaI AGT'ACT
Cuts at: 6022 7734 6022
Size: 1712 6062
SfcI C'TryA_G
Cuts at: 826 1045 2679 3189 3954 4569 4839 5064 5122
Size: 219 1634 510 765 615 270 225 58
Cuts at: 5122 6257 6938 7129 826
Size: 1135 681 191 1471
Fragments arranged by size:
1634 1471 1135 765 681 615 510 270
225 219 191 58
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7774
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7774
SmaI CCC'GGG
Cuts at: 1872 5101 1872
Size: 3229 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2366 2925 3019 3635 4496 5878
Size: 197 926 423 559 94 616 861 1382
Cuts at: 5878 6779 7041 820
Size: 901 262 1553
Fragments arranged by size:
1553 1382 926 901 861 616 559 423
262 197 94
SnaBI TAC'GTA
Cuts at: 493 5565 493
Size: 5072 2702
SpeI A'CTAG_T
Cuts at: 152 3538 152
Size: 3386 4388
SphI G_CATG'C
Cuts at: 1132 5132 1132
Size: 4000 3774
SspI AAT'ATT
Cuts at: 5 52 2142 2598 4741 5698 5
Size: 47 2090 456 2143 957 2081
Fragments arranged by size:
2143 2090 2081 957 456 47
StuI AGG'CCT
Cuts at: 1517 4339 1517
Size: 2822 4952
StyI C'CwwG_G
Cuts at: 513 1065 3200 4334 4887 5213 513
Size: 552 2135 1134 553 326 3074
Fragments arranged by size:
3074 2135 1134 553 552 326
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4821 5801 5818 5971 6156 6601 325
Size: 1255 3241 980 17 153 185 445 1498
Fragments arranged by size:
3241 1498 1255 980 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2686 3160 4304
Size: 274 80 33 51 1443 709 474 1144
Cuts at: 4304 6020 7732 96
Size: 1716 1712 138
Fragments arranged by size:
1716 1712 1443 1144 709 474 274 138
80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 7734 7766 7734
Size: 32 7742
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 7774
XbaI T'CTAG_A
Cuts at: 5110 5110
Size: 7774
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3787 3787
Size: 7774
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7774
XmaI C'CCGG_G
Cuts at: 1870 5099 1870
Size: 3229 4545
XmnI GAAnn'nnTTC
Cuts at: 2911 3294 4423 4540 4784 5903 2911
Size: 383 1129 117 244 1119 4782
Fragments arranged by size:
4782 1129 1119 383 244 117
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5582 276
Size: 53 83 186 936 4048 2468
Fragments arranged by size:
4048 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI AvrII BaeI BamHI BanI BanII BbsI
BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI
Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI
EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI
MluI MmeI MscI MslI MspA1I NcoI NdeI NheI
NotI NsiI NspI PasI PciI PflMI PfoI PmeI
PpiI PpuMI PshAI PsiI PstI PvuI PvuII SalI
SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI BbeI BbvCI Bpu10I BsiWI
BsmBI BstXI BstZ17I DraIII EcoICRI EcoNI EcoRV FseI
FspAI KasI NaeI NarI NgoMIV NruI PacI PmlI
PspOMI PspXI PsrI RsrII SacI SacII SanDI SapI
SexAI SfiI SfoI SrfI SwaI