Restriction map for
pFN21AA0011
(Circular) (Six-base) MAPSORT of: pFN21AA0011.seq Check: 5253 from: 1 to: 7438
pFN21AA0011 7438 bp
With 182 enzymes: SgfI *
February 27, 2009 14:36 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3619 3619
Size: 7438
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3940 5248 278
Size: 53 83 186 936 2404 1308 2468
Fragments arranged by size:
2468 2404 1308 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2073 3541 4781 1937
Size: 136 1468 1240 4594
Fragments arranged by size:
4594 1468 1240 136
Acc65I G'GTAC_C
Cuts at: 4759 4759
Size: 7438
AclI AA'CG_TT
Cuts at: 1563 5565 5938 1563
Size: 4002 373 3063
Fragments arranged by size:
4002 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2106 2244 2486 2573 2622 2787 2838 3123
Size: 474 138 242 87 49 165 51 285
Cuts at: 3123 4323 5501 6885 7153 1632
Size: 1200 1178 1384 268 1917
Fragments arranged by size:
1917 1384 1200 1178 474 285 268 242
165 138 87 51 49
AfeI AGC'GCT
Cuts at: 3161 3968 3161
Size: 807 6631
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 7241
AflIII A'CryG_T
Cuts at: 1203 6337 7314 1203
Size: 5134 977 1327
Fragments arranged by size:
5134 1327 977
AgeI A'CCGG_T
Cuts at: 6269 6269
Size: 7438
AhdI GACnn_n'nnGTC
Cuts at: 6167 6167
Size: 7438
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 7438
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 7438
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3410 3442 3410
Size: 32 7406
AlwNI CAG_nnn'CTG
Cuts at: 2596 5181 6753 2596
Size: 2585 1572 3281
Fragments arranged by size:
3281 2585 1572
ApaI G_GGCC'C
Cuts at: 2651 2792 3376 2651
Size: 141 584 6713
Fragments arranged by size:
6713 584 141
ApaLI G'TGCA_C
Cuts at: 5495 6651 5495
Size: 1156 6282
ApoI r'AATT_y
Cuts at: 1493 3920 4747 4973 5125 7234 1493
Size: 2427 827 226 152 2109 1697
Fragments arranged by size:
2427 2109 1697 827 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5992 160
Size: 872 4960 1606
Fragments arranged by size:
4960 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7438
AvaI C'yCGr_G
Cuts at: 1870 1943 4763 1870
Size: 73 2820 4545
Fragments arranged by size:
4545 2820 73
AvrII C'CTAG_G
Cuts at: 2285 2285
Size: 7438
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2567 2600 2567
Size: 33 7405
BamHI G'GATC_C
Cuts at: 4142 4768 5135 4142
Size: 626 367 6445
Fragments arranged by size:
6445 626 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3110 3210 3458 4121 4759
Size: 325 217 627 1323 100 248 663 638
Cuts at: 4759 6214 618
Size: 1455 1842
Fragments arranged by size:
1842 1455 1323 663 638 627 325 248
217 100
BanII G_rGCy'C
Cuts at: 1391 2610 2651 2792 2804 3202 3376 4191 4654
Size: 1219 41 141 12 398 174 815 463
Cuts at: 4654 4757 1391
Size: 103 4072
Fragments arranged by size:
4072 1219 815 463 398 174 141 103
41 12
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2748 928
Size: 971 849 5618
Fragments arranged by size:
5618 971 849
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3219 3253 5629 5663 1903
Size: 34 1282 34 2376 34 3678
Fragments arranged by size:
3678 2376 1282 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2002 3777 5330 6540 2002
Size: 1775 1553 1210 2900
Fragments arranged by size:
2900 1775 1553 1210
BclI T'GATC_A
Cuts at: 1548 2197 1548
Size: 649 6789
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2573 3563 3700
Size: 107 122 71 631 605 901 990 137
Cuts at: 3700 6049 136
Size: 2349 1525
Fragments arranged by size:
2349 1525 990 901 631 605 137 122
107 71
BglII A'GATC_T
Cuts at: 2251 4276 7433 2251
Size: 2025 3157 2256
Fragments arranged by size:
3157 2256 2025
BlpI GC'TnA_GC
Cuts at: 3567 4855 3567
Size: 1288 6150
Bme1580I G_kGCm'C
Cuts at: 1790 2651 2792 2829 3215 3376 5499 6655 1790
Size: 861 141 37 386 161 2123 1156 2573
Fragments arranged by size:
2573 2123 1156 861 386 161 141 37
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 7438
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2986 3209 3429
Size: 416 53 83 399 24 1569 223 220
Cuts at: 3429 4631 5169 6127 442
Size: 1202 538 958 1753
Fragments arranged by size:
1753 1569 1202 958 538 416 399 223
220 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7438
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2229 2229
Size: 7438
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2199 2218 2738 3244 3305 4526 6098 1239
Size: 960 19 520 506 61 1221 1572 2579
Fragments arranged by size:
2579 1572 1221 960 520 506 61 19
Bpu10I CC'TnA_GC
Cuts at: 2814 3677 4023 2814
Size: 863 346 6229
Fragments arranged by size:
6229 863 346
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3053 3407 3994 5563 6428 6726 3053
Size: 354 587 1569 865 298 3765
Fragments arranged by size:
3765 1569 865 587 354 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2281 2390 2525 3500 6101 882
Size: 617 782 109 135 975 2601 2219
Fragments arranged by size:
2601 2219 975 782 617 135 109
BsaAI yAC'GTr
Cuts at: 493 1204 5229 493
Size: 711 4025 2702
Fragments arranged by size:
4025 2702 711
BsaBI GATnn'nnATC
Cuts at: 7432 7432
Size: 7438
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3937 5245 5627 6262
Size: 53 83 186 936 2404 1308 382 635
Cuts at: 6262 275
Size: 1451
Fragments arranged by size:
2404 1451 1308 936 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 3550 3909 5871 6269 6543 6690 7101 3550
Size: 359 1962 398 274 147 411 3887
Fragments arranged by size:
3887 1962 411 398 359 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2703 2733 3050 3080 4529
Size: 30 165 30 1300 30 317 30 1449
Cuts at: 4529 4559 1178
Size: 30 4057
Fragments arranged by size:
4057 1449 1300 317 165 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2218 3074 3465 4101 1186
Size: 1032 856 391 636 4523
Fragments arranged by size:
4523 1032 856 636 391
BseYI C'CCAG_C
Cuts at: 3162 6641 7137 3162
Size: 3479 496 3463
Fragments arranged by size:
3479 3463 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2157 1163
Size: 573 421 6444
Fragments arranged by size:
6444 573 421
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4888 5649 6677 1540
Size: 275 186 2887 761 1028 2301
Fragments arranged by size:
2887 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2610 2731 2804 4191 5499 5584 6655 2610
Size: 121 73 1387 1308 85 1071 3393
Fragments arranged by size:
3393 1387 1308 1071 121 85 73
BsmI GAATG_Cn'
Cuts at: 4474 4949 5042 4474
Size: 475 93 6870
Fragments arranged by size:
6870 475 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2610 2651 2731 2792 2804 2829 3202
Size: 399 820 41 80 61 12 25 373
Cuts at: 3202 3215 3376 4191 4654 4757 5499 5584 6655
Size: 13 161 815 463 103 742 85 1071
Cuts at: 6655 1391
Size: 2174
Fragments arranged by size:
2174 1071 820 815 742 463 399 373
161 103 85 80 61 41 25 13
12
BspEI T'CCGG_A
Cuts at: 3550 7101 3550
Size: 3551 3887
BspHI T'CATG_A
Cuts at: 5327 7057 5327
Size: 1730 5708
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2021 3057 3619 4793 844
Size: 1177 1036 562 1174 3489
Fragments arranged by size:
3489 1177 1174 1036 562
BsrBI CCG'CTC
Cuts at: 1394 1823 3041 3193 5325 1394
Size: 429 1218 152 2132 3507
Fragments arranged by size:
3507 2132 1218 429 152
BsrDI GCAATG_nn'
Cuts at: 65 1248 4191 5933 6107 65
Size: 1183 2943 1742 174 1396
Fragments arranged by size:
2943 1742 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2045 6082 6269 1700
Size: 110 235 4037 187 2869
Fragments arranged by size:
4037 2869 235 187 110
BsrGI T'GTAC_A
Cuts at: 96 3082 96
Size: 2986 4452
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7438
BssSI C'ACGA_G
Cuts at: 2841 5498 6510 2841
Size: 2657 1012 3769
Fragments arranged by size:
3769 2657 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 7438
BstBI TT'CG_AA
Cuts at: 4588 6242 4588
Size: 1654 5784
BstEII G'GTnAC_C
Cuts at: 1184 4079 1184
Size: 2895 4543
BstXI CCAn_nnnn'nTGG
Cuts at: 3162 3162
Size: 7438
BstYI r'GATC_y
Cuts at: 1972 2251 2682 2846 4142 4276 4768 5135 5520
Size: 279 431 164 1296 134 492 367 385
Cuts at: 5520 5537 6989 7075 7087 7433 1972
Size: 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 1296 492 431 385 367 346
279 164 134 86 17 12
Bsu36I CC'TnA_GG
Cuts at: 2520 2643 2520
Size: 123 7315
BtgI C'CryG_G
Cuts at: 513 1065 2004 2052 2732 4603 513
Size: 552 939 48 680 1871 3348
Fragments arranged by size:
3348 1871 939 680 552 48
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 4703 487
Size: 683 56 3477 3222
Fragments arranged by size:
3477 3222 683 56
BtsI GCAGTG_nn'
Cuts at: 2872 3591 4104 4954 5748 5768 2872
Size: 719 513 850 794 20 4542
Fragments arranged by size:
4542 850 794 719 513 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7438
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 7403
DraI TTT'AAA
Cuts at: 4742 4742
Size: 7438
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 6445 809
Size: 507 5129 1802
Fragments arranged by size:
5129 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2302 2489 4885
Size: 54 1425 50 275 18 472 187 2396
Cuts at: 4885 5774 7403 8
Size: 889 1629 43
Fragments arranged by size:
2396 1629 1425 889 472 275 187 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4885 1537
Size: 275 3073 4090
Fragments arranged by size:
4090 3073 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2125 2303 2598 2709 5368 1353
Size: 772 178 295 111 2659 3423
Fragments arranged by size:
3423 2659 772 295 178 111
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 6025 6399 6545 387
Size: 1064 138 4436 374 146 1280
Fragments arranged by size:
4436 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2608 2802 4189 2608
Size: 194 1387 5857
Fragments arranged by size:
5857 1387 194
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2106 2199 2218 2244 2486 2573 2622
Size: 393 474 93 19 26 242 87 49
Cuts at: 2622 2738 2787 2838 3123 3244 3305 4323 4526
Size: 116 49 51 285 121 61 1018 203
Cuts at: 4526 5501 6098 6885 7153 1239
Size: 975 597 787 268 1524
Fragments arranged by size:
1524 1018 975 787 597 474 393 285
268 242 203 121 116 93 87 61
51 49 49 26 19
EcoNI CCTnn'n_nnAGG
Cuts at: 2039 3053 4116 2039
Size: 1014 1063 5361
Fragments arranged by size:
5361 1063 1014
EcoO109I rG'GnC_Cy
Cuts at: 2289 2333 2343 2514 2647 2648 2788 3308 3331
Size: 44 10 171 133 1 140 520 23
Cuts at: 3331 3339 3372 3418 3556 3702 3928 4336 2289
Size: 8 33 46 138 146 226 408 5391
Fragments arranged by size:
5391 520 408 226 171 146 140 138
133 46 44 33 23 10 8 1
EcoRI G'AATT_C
Cuts at: 4747 5125 4747
Size: 378 7060
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2594 3034 3988 4117 2594
Size: 440 954 129 5915
Fragments arranged by size:
5915 954 440 129
FseI GG_CCGG'CC
Cuts at: 2049 2049
Size: 7438
FspI TGC'GCA
Cuts at: 4454 5944 4454
Size: 1490 5948
HaeII r_GCGC'y
Cuts at: 1678 3163 3970 6585 1678
Size: 1485 807 2615 2531
Fragments arranged by size:
2615 2531 1485 807
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2789 2821 3050 3082 4273
Size: 32 135 32 1217 32 229 32 1191
Cuts at: 4273 4305 6079 6111 6153 6185 1373
Size: 32 1774 32 42 32 2626
Fragments arranged by size:
2626 1774 1217 1191 229 135 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 4378 4782 5028 669
Size: 988 281 2440 404 246 3079
Fragments arranged by size:
3079 2440 988 404 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 7438
HpaI GTT'AAC
Cuts at: 5028 5028
Size: 7438
KpnI G_GTAC'C
Cuts at: 4763 4763
Size: 7438
MfeI C'AATT_G
Cuts at: 5037 5037
Size: 7438
MluI A'CGCG_T
Cuts at: 7314 7314
Size: 7438
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2424 4170 4696 4733 6552 6736 1305
Size: 1119 1746 526 37 1819 184 2007
Fragments arranged by size:
2007 1819 1746 1119 526 184 37
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 5616
Fragments arranged by size:
5616 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2066 2417 4659 5396 5755 5914 518
Size: 648 900 351 2242 737 359 159 2042
Fragments arranged by size:
2242 2042 900 737 648 359 351 159
MspA1I CmG'CkG
Cuts at: 1606 3781 4504 4854 5531 6679 6924 1606
Size: 2175 723 350 677 1148 245 2120
Fragments arranged by size:
2175 2120 1148 723 677 350 245
NaeI GCC'GGC
Cuts at: 2047 2047
Size: 7438
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 5947
Fragments arranged by size:
5947 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7438
NgoMIV G'CCGG_C
Cuts at: 2045 2045
Size: 7438
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7438
NotI GC'GGCC_GC
Cuts at: 4885 4885
Size: 7438
NspI r_CATG'y
Cuts at: 1132 1229 4459 4796 6341 1132
Size: 97 3230 337 1545 2229
Fragments arranged by size:
3230 2229 1545 337 97
PasI CC'CwG_GG
Cuts at: 1348 2035 1348
Size: 687 6751
PciI A'CATG_T
Cuts at: 6337 6337
Size: 7438
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 7438
PfoI T'CCnGG_A
Cuts at: 2080 2080
Size: 7438
PmeI GTTT'AAAC
Cuts at: 4742 4742
Size: 7438
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5507 5539 7020 7052 5507
Size: 32 1481 32 5893
Fragments arranged by size:
5893 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 3308 3339 3418 3928 4336 3308
Size: 31 79 510 408 6410
Fragments arranged by size:
6410 510 408 79 31
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7438
PsiI TTA'TAA
Cuts at: 3732 5008 3732
Size: 1276 6162
PspOMI G'GGCC_C
Cuts at: 2647 2788 3372 2647
Size: 141 584 6713
Fragments arranged by size:
6713 584 141
PstI C_TGCA'G
Cuts at: 830 3630 3780 4171 4503 4790 830
Size: 2800 150 391 332 287 3478
Fragments arranged by size:
3478 2800 391 332 287 150
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7438
PvuII CAG'CTG
Cuts at: 4504 4504
Size: 7438
SacI G_AGCT'C
Cuts at: 2610 2804 4191 2610
Size: 194 1387 5857
Fragments arranged by size:
5857 1387 194
SalI G'TCGA_C
Cuts at: 1936 4780 1936
Size: 2844 4594
SanDI GG'GwC_CC
Cuts at: 4336 4336
Size: 7438
SapI GCTCTTCn'nnn_
Cuts at: 2598 2598
Size: 7438
SbfI CC_TGCA'GG
Cuts at: 4790 4790
Size: 7438
ScaI AGT'ACT
Cuts at: 3528 5686 7398 3528
Size: 2158 1712 3568
Fragments arranged by size:
3568 2158 1712
SfcI C'TryA_G
Cuts at: 826 1045 2820 3626 3648 3776 4167 4245 4499
Size: 219 1775 806 22 128 391 78 254
Cuts at: 4499 4786 5921 6602 6793 826
Size: 287 1135 681 191 1471
Fragments arranged by size:
1775 1471 1135 806 681 391 287 254
219 191 128 78 22
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7438
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7438
SmaI CCC'GGG
Cuts at: 1872 4765 1872
Size: 2893 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3032 3422 3973 5542 6443 6705
Size: 197 926 1089 390 551 1569 901 262
Cuts at: 6705 820
Size: 1553
Fragments arranged by size:
1569 1553 1089 926 901 551 390 262
197
SnaBI TAC'GTA
Cuts at: 493 5229 493
Size: 4736 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7438
SphI G_CATG'C
Cuts at: 1132 4459 4796 1132
Size: 3327 337 3774
Fragments arranged by size:
3774 3327 337
SspI AAT'ATT
Cuts at: 5 52 5362 5
Size: 47 5310 2081
Fragments arranged by size:
5310 2081 47
StuI AGG'CCT
Cuts at: 1517 4097 1517
Size: 2580 4858
StyI C'CwwG_G
Cuts at: 513 1065 2004 2285 2445 2652 2946 3334 4084
Size: 552 939 281 160 207 294 388 750
Cuts at: 4084 4350 4877 513
Size: 266 527 3074
Fragments arranged by size:
3074 939 750 552 527 388 294 281
266 207 160
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3300 5465 5482 5635 5820 6265 325
Size: 1255 1720 2165 17 153 185 445 1498
Fragments arranged by size:
2165 1720 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3082 3526 3977
Size: 274 80 33 51 1443 1105 444 451
Cuts at: 3977 5684 7396 96
Size: 1707 1712 138
Fragments arranged by size:
1712 1707 1443 1105 451 444 274 138
80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2076 2108 2299 2331 2703 2735 3136 3168 3382
Size: 32 191 32 372 32 401 32 214
Cuts at: 3382 3414 7398 7430 2076
Size: 32 3984 32 2084
Fragments arranged by size:
3984 2084 401 372 214 191 32 32
32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 7438
XbaI T'CTAG_A
Cuts at: 4774 4774
Size: 7438
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3383 4601 3383
Size: 1218 6220
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7438
XmaI C'CCGG_G
Cuts at: 1870 4763 1870
Size: 2893 4545
XmnI GAAnn'nnTTC
Cuts at: 5567 5567
Size: 7438
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3938 5246 276
Size: 53 83 186 936 2404 1308 2468
Fragments arranged by size:
2468 2404 1308 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI
ApaLI ApoI AseI AsiSI AvaI AvrII BaeI BamHI
BanI BanII BbsI BcgI BciVI BclI BglI BglII
BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FseI FspI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV
NheI NotI NspI PasI PciI PflMI PfoI PmeI
PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII
SacI SalI SanDI SapI SbfI ScaI SfcI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AscI BbeI BbvCI BfrBI BsiWI BsmBI BstZ17I DraIII
EcoRV FspAI KasI NarI NruI NsiI PacI PmlI
PspXI PsrI RsrII SacII SexAI SfiI SfoI SrfI
SwaI