Restriction map for
pFN21AA0008
(Circular) (Six-base) MAPSORT of: pFN21AA0008.seq Check: 9576 from: 1 to: 7243
pFN21AA0008 7243 bp
With 182 enzymes: SgfI *
January 16, 2009 15:22 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5053 278
Size: 53 83 186 936 3517 2468
Fragments arranged by size:
3517 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2029 4586 1937
Size: 92 2557 4594
Fragments arranged by size:
4594 2557 92
Acc65I G'GTAC_C
Cuts at: 4564 4564
Size: 7243
AclI AA'CG_TT
Cuts at: 1563 5370 5743 1563
Size: 3807 373 3063
Fragments arranged by size:
3807 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2984 3915 5306 6690 6958 1632
Size: 1352 931 1391 1384 268 1917
Fragments arranged by size:
1917 1391 1384 1352 931 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 7046
AflIII A'CryG_T
Cuts at: 1203 3181 4027 6142 7119 1203
Size: 1978 846 2115 977 1327
Fragments arranged by size:
2115 1978 1327 977 846
AgeI A'CCGG_T
Cuts at: 6074 6074
Size: 7243
AhdI GACnn_n'nnGTC
Cuts at: 3884 5972 3884
Size: 2088 5155
AleI CACnn'nnGTG
Cuts at: 1166 3288 1166
Size: 2122 5121
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 7243
AlwNI CAG_nnn'CTG
Cuts at: 2953 3326 3797 4986 6558 2953
Size: 373 471 1189 1572 3638
Fragments arranged by size:
3638 1572 1189 471 373
ApaLI G'TGCA_C
Cuts at: 5300 6456 5300
Size: 1156 6087
ApoI r'AATT_y
Cuts at: 1493 2178 2818 2917 3078 3214 3572 4236 4267
Size: 685 640 99 161 136 358 664 31
Cuts at: 4267 4359 4538 4552 4778 4930 7039 1493
Size: 92 179 14 226 152 2109 1697
Fragments arranged by size:
2109 1697 685 664 640 358 226 179
161 152 136 99 92 31 14
AseI AT'TA_AT
Cuts at: 160 1032 4215 5797 160
Size: 872 3183 1582 1606
Fragments arranged by size:
3183 1606 1582 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7243
AvaI C'yCGr_G
Cuts at: 1870 1943 4568 1870
Size: 73 2625 4545
Fragments arranged by size:
4545 2625 73
BamHI G'GATC_C
Cuts at: 4573 4940 4573
Size: 367 6876
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2728 4564 6019 618
Size: 325 217 627 941 1836 1455 1842
Fragments arranged by size:
1842 1836 1455 941 627 325 217
BanII G_rGCy'C
Cuts at: 1391 4562 1391
Size: 3171 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2001 2749 2972 4043 928
Size: 971 102 748 223 1071 4128
Fragments arranged by size:
4128 1071 971 748 223 102
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5434 5468 1903
Size: 34 3497 34 3678
Fragments arranged by size:
3678 3497 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 5135 6345 5135
Size: 1210 6033
BclI T'GATC_A
Cuts at: 1548 2266 3585 1548
Size: 718 1319 5206
Fragments arranged by size:
5206 1319 718
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5854 136
Size: 107 122 71 631 605 4182 1525
Fragments arranged by size:
4182 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 3397 3517 7238 3397
Size: 120 3721 3402
Fragments arranged by size:
3721 3402 120
BlpI GC'TnA_GC
Cuts at: 2424 3276 4660 2424
Size: 852 1384 5007
Fragments arranged by size:
5007 1384 852
Bme1580I G_kGCm'C
Cuts at: 1790 5304 6460 1790
Size: 3514 1156 2573
Fragments arranged by size:
3514 2573 1156
BmgBI CAC'GTC
Cuts at: 1318 2607 1318
Size: 1289 5954
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3015 4974 5932
Size: 416 53 83 399 24 1598 1959 958
Cuts at: 5932 442
Size: 1753
Fragments arranged by size:
1959 1753 1598 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7243
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2637 4200 2637
Size: 1563 5680
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3299 4478 5903 1239
Size: 2060 1179 1425 2579
Fragments arranged by size:
2579 2060 1425 1179
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2190 2619 3048 3614 5368 6233 6531 2190
Size: 429 429 566 1754 865 298 2902
Fragments arranged by size:
2902 1754 865 566 429 429 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5906 882
Size: 617 4407 2219
Fragments arranged by size:
4407 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 5034 493
Size: 711 3830 2702
Fragments arranged by size:
3830 2702 711
BsaBI GATnn'nnATC
Cuts at: 7237 7237
Size: 7243
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 5050 5432 6067 275
Size: 53 83 186 936 3517 382 635 1451
Fragments arranged by size:
3517 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 5676 6074 6348 6495 6906 5676
Size: 398 274 147 411 6013
Fragments arranged by size:
6013 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2268 2298 3019 3049 4527
Size: 30 165 30 865 30 721 30 1478
Cuts at: 4527 4557 1178
Size: 30 3864
Fragments arranged by size:
3864 1478 865 721 165 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 4477 1186
Size: 3291 3952
BseYI C'CCAG_C
Cuts at: 6446 6942 6446
Size: 496 6747
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2609 1163
Size: 573 873 5797
Fragments arranged by size:
5797 873 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4693 5454 6482 1540
Size: 275 186 2692 761 1028 2301
Fragments arranged by size:
2692 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 5304 5389 6460 5304
Size: 85 1071 6087
Fragments arranged by size:
6087 1071 85
BsmI GAATG_Cn'
Cuts at: 2416 3969 4409 4754 4847 2416
Size: 1553 440 345 93 4812
Fragments arranged by size:
4812 1553 440 345 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 4562 5304 5389 6460 1391
Size: 399 2772 742 85 1071 2174
Fragments arranged by size:
2772 2174 1071 742 399 85
BspEI T'CCGG_A
Cuts at: 6906 6906
Size: 7243
BspHI T'CATG_A
Cuts at: 5132 6862 5132
Size: 1730 5513
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2638 2755 4598 844
Size: 1794 117 1843 3489
Fragments arranged by size:
3489 1843 1794 117
BsrBI CCG'CTC
Cuts at: 1394 1823 5130 1394
Size: 429 3307 3507
Fragments arranged by size:
3507 3307 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2253 3343 3756 5738 5912 65
Size: 1183 1005 1090 413 1982 174 1396
Fragments arranged by size:
1982 1396 1183 1090 1005 413 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4001 5887 6074 1700
Size: 110 2191 1886 187 2869
Fragments arranged by size:
2869 2191 1886 187 110
BsrGI T'GTAC_A
Cuts at: 96 3512 96
Size: 3416 3827
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7243
BssSI C'ACGA_G
Cuts at: 5303 6315 5303
Size: 1012 6231
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 7243
BstBI TT'CG_AA
Cuts at: 6047 6047
Size: 7243
BstEII G'GTnAC_C
Cuts at: 1184 4502 1184
Size: 3318 3925
BstYI r'GATC_y
Cuts at: 1972 3397 3517 4573 4940 5325 5342 6794 6880
Size: 1425 120 1056 367 385 17 1452 86
Cuts at: 6880 6892 7238 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 1425 1056 385 367 346 120
86 17 12
Bsu36I CC'TnA_GG
Cuts at: 2931 2931
Size: 7243
BtgI C'CryG_G
Cuts at: 513 1065 3286 513
Size: 552 2221 4470
Fragments arranged by size:
4470 2221 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6504
Fragments arranged by size:
6504 683 56
BtsI GCAGTG_nn'
Cuts at: 4759 5553 5573 4759
Size: 794 20 6429
Fragments arranged by size:
6429 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7243
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 7208
DraI TTT'AAA
Cuts at: 2359 3042 3189 3219 4547 2359
Size: 683 147 30 1328 5055
Fragments arranged by size:
5055 1328 683 147 30
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 6250 809
Size: 507 4934 1802
Fragments arranged by size:
4934 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 4690 5579 7208
Size: 54 1425 50 275 18 2860 889 1629
Cuts at: 7208 8
Size: 43
Fragments arranged by size:
2860 1629 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 4690 1537
Size: 275 2878 4090
Fragments arranged by size:
4090 2878 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3895 4191 5173 1353
Size: 2542 296 982 3423
Fragments arranged by size:
3423 2542 982 296
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5830 6204 6350 387
Size: 1064 138 4241 374 146 1280
Fragments arranged by size:
4241 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2984 3299 3915 4478 5306 5903 6690
Size: 393 1352 315 616 563 828 597 787
Cuts at: 6690 6958 1239
Size: 268 1524
Fragments arranged by size:
1524 1352 828 787 616 597 563 393
315 268
EcoNI CCTnn'n_nnAGG
Cuts at: 3437 3437
Size: 7243
EcoO109I rG'GnC_Cy
Cuts at: 3155 3899 3155
Size: 744 6499
EcoRI G'AATT_C
Cuts at: 2178 2818 2917 4267 4552 4930 2178
Size: 640 99 1350 285 378 4491
Fragments arranged by size:
4491 1350 640 378 285 99
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2565 2938 4159 2565
Size: 373 1221 5649
Fragments arranged by size:
5649 1221 373
FspI TGC'GCA
Cuts at: 5749 5749
Size: 7243
HaeII r_GCGC'y
Cuts at: 1678 6390 1678
Size: 4712 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2186 2218 3870 3902 4145
Size: 32 135 32 614 32 1652 32 243
Cuts at: 4145 4177 4453 4485 5884 5916 5958 5990 1373
Size: 32 276 32 1399 32 42 32 2626
Fragments arranged by size:
2626 1652 1399 614 276 243 135 42
32 32 32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2030 3934 4587 4833 669
Size: 988 281 92 1904 653 246 3079
Fragments arranged by size:
3079 1904 988 653 281 246 92
HindIII A'AGCT_T
Cuts at: 748 748
Size: 7243
HpaI GTT'AAC
Cuts at: 4833 4833
Size: 7243
KpnI G_GTAC'C
Cuts at: 4568 4568
Size: 7243
MfeI C'AATT_G
Cuts at: 4842 4842
Size: 7243
MluI A'CGCG_T
Cuts at: 7119 7119
Size: 7243
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2186 2554 6357 6541 1305
Size: 881 368 3803 184 2007
Fragments arranged by size:
3803 2007 881 368 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 5421
Fragments arranged by size:
5421 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3091 3288 5201 5560 5719 518
Size: 648 1925 197 1913 359 159 2042
Fragments arranged by size:
2042 1925 1913 648 359 197 159
MspA1I CmG'CkG
Cuts at: 1606 4456 4659 5336 6484 6729 1606
Size: 2850 203 677 1148 245 2120
Fragments arranged by size:
2850 2120 1148 677 245 203
NcoI C'CATG_G
Cuts at: 513 1065 3286 513
Size: 552 2221 4470
Fragments arranged by size:
4470 2221 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7243
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7243
NotI GC'GGCC_GC
Cuts at: 4690 4690
Size: 7243
NspI r_CATG'y
Cuts at: 1132 1229 3185 4031 4481 4601 6146 1132
Size: 97 1956 846 450 120 1545 2229
Fragments arranged by size:
2229 1956 1545 846 450 120 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 7243
PciI A'CATG_T
Cuts at: 3181 4027 6142 3181
Size: 846 2115 4282
Fragments arranged by size:
4282 2115 846
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2957 1108
Size: 1849 5394
PfoI T'CCnGG_A
Cuts at: 4093 4093
Size: 7243
PmeI GTTT'AAAC
Cuts at: 4547 4547
Size: 7243
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5312 5344 6825 6857 5312
Size: 32 1481 32 5698
Fragments arranged by size:
5698 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 3155 3155
Size: 7243
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7243
PsiI TTA'TAA
Cuts at: 4813 4813
Size: 7243
PstI C_TGCA'G
Cuts at: 830 4439 4595 830
Size: 3609 156 3478
Fragments arranged by size:
3609 3478 156
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7243
PvuII CAG'CTG
Cuts at: 4456 4456
Size: 7243
SalI G'TCGA_C
Cuts at: 1936 2028 4585 1936
Size: 92 2557 4594
Fragments arranged by size:
4594 2557 92
SbfI CC_TGCA'GG
Cuts at: 4595 4595
Size: 7243
ScaI AGT'ACT
Cuts at: 2050 5491 7203 2050
Size: 3441 1712 2090
Fragments arranged by size:
3441 2090 1712
SexAI A'CCwGG_T
Cuts at: 2533 4423 2533
Size: 1890 5353
SfcI C'TryA_G
Cuts at: 826 1045 4435 4591 5726 6407 6598 826
Size: 219 3390 156 1135 681 191 1471
Fragments arranged by size:
3390 1471 1135 681 219 191 156
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7243
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7243
SmaI CCC'GGG
Cuts at: 1872 4570 1872
Size: 2698 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2205 2634 3063 3593 5347 6248
Size: 197 926 262 429 429 530 1754 901
Cuts at: 6248 6510 820
Size: 262 1553
Fragments arranged by size:
1754 1553 926 901 530 429 429 262
262 197
SnaBI TAC'GTA
Cuts at: 493 5034 493
Size: 4541 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7243
SphI G_CATG'C
Cuts at: 1132 4601 1132
Size: 3469 3774
SspI AAT'ATT
Cuts at: 5 52 2355 5167 5
Size: 47 2303 2812 2081
Fragments arranged by size:
2812 2303 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 7243
StyI C'CwwG_G
Cuts at: 513 1065 2166 2240 3286 4682 513
Size: 552 1101 74 1046 1396 3074
Fragments arranged by size:
3074 1396 1101 1046 552 74
SwaI ATTT'AAAT
Cuts at: 3219 3219
Size: 7243
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3034 3594 5270 5287 5440 5625 6070
Size: 1255 1454 560 1676 17 153 185 445
Cuts at: 6070 325
Size: 1498
Fragments arranged by size:
1676 1498 1454 1255 560 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2048 3512 5489
Size: 274 80 33 51 1443 71 1464 1977
Cuts at: 5489 7201 96
Size: 1712 138
Fragments arranged by size:
1977 1712 1464 1443 274 138 80 71
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3019 3051 3590 3622 7203 7235 3019
Size: 32 539 32 3581 32 3027
Fragments arranged by size:
3581 3027 539 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2535 1316
Size: 1219 6024
XbaI T'CTAG_A
Cuts at: 4579 4579
Size: 7243
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2482 2482
Size: 7243
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7243
XmaI C'CCGG_G
Cuts at: 1870 4568 1870
Size: 2698 4545
XmnI GAAnn'nnTTC
Cuts at: 2921 5372 2921
Size: 2451 4792
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5051 276
Size: 53 83 186 936 3517 2468
Fragments arranged by size:
3517 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NcoI NdeI NheI NotI NspI PasI
PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI
PstI PvuI PvuII SalI SbfI ScaI SexAI SfcI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI SwaI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI Bpu10I BsiWI BsmBI BstXI BstZ17I DraIII
EcoICRI EcoRV FseI FspAI KasI NaeI NarI NgoMIV
NruI NsiI PacI PmlI PspOMI PspXI PsrI RsrII
SacI SacII SanDI SapI SfiI SfoI SrfI