Restriction map for
pFN21AA0003
(Circular) (Six-base) MAPSORT of: pFN21AA0003.seq Check: 3603 from: 1 to: 6199
pFN21AA0003 6199 bp
With 182 enzymes: SgfI *
February 27, 2009 14:36 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4009 278
Size: 53 83 186 936 2473 2468
Fragments arranged by size:
2473 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3542 1937
Size: 1605 4594
Acc65I G'GTAC_C
Cuts at: 3520 3520
Size: 6199
AclI AA'CG_TT
Cuts at: 1563 4326 4699 1563
Size: 2763 373 3063
Fragments arranged by size:
3063 2763 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2729 4262 5646 5914 1632
Size: 1097 1533 1384 268 1917
Fragments arranged by size:
1917 1533 1384 1097 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6002
AflIII A'CryG_T
Cuts at: 1203 2221 2266 5098 6075 1203
Size: 1018 45 2832 977 1327
Fragments arranged by size:
2832 1327 1018 977 45
AgeI A'CCGG_T
Cuts at: 5030 5030
Size: 6199
AhdI GACnn_n'nnGTC
Cuts at: 4928 4928
Size: 6199
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6199
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2229 1184
Size: 1045 5154
AlwNI CAG_nnn'CTG
Cuts at: 2293 2494 2701 2758 3942 5514 2293
Size: 201 207 57 1184 1572 2978
Fragments arranged by size:
2978 1572 1184 207 201 57
ApaI G_GGCC'C
Cuts at: 3450 3450
Size: 6199
ApaLI G'TGCA_C
Cuts at: 4256 5412 4256
Size: 1156 5043
ApoI r'AATT_y
Cuts at: 1493 2500 3508 3734 3886 5995 1493
Size: 1007 1008 226 152 2109 1697
Fragments arranged by size:
2109 1697 1008 1007 226 152
AseI AT'TA_AT
Cuts at: 160 1032 2916 4753 160
Size: 872 1884 1837 1606
Fragments arranged by size:
1884 1837 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6199
AvaI C'yCGr_G
Cuts at: 1870 1943 3524 1870
Size: 73 1581 4545
Fragments arranged by size:
4545 1581 73
BamHI G'GATC_C
Cuts at: 3529 3896 3529
Size: 367 5832
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3520 4975 618
Size: 325 217 627 1733 1455 1842
Fragments arranged by size:
1842 1733 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 3450 3518 1391
Size: 2059 68 4072
Fragments arranged by size:
4072 2059 68
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5228
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4390 4424 1903
Size: 34 2453 34 3678
Fragments arranged by size:
3678 2453 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4091 5301 4091
Size: 1210 4989
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6199
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4810 136
Size: 107 122 71 631 605 3138 1525
Fragments arranged by size:
3138 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6194 6194
Size: 6199
BlpI GC'TnA_GC
Cuts at: 3616 3616
Size: 6199
Bme1580I G_kGCm'C
Cuts at: 1790 3325 3450 4260 5416 1790
Size: 1535 125 810 1156 2573
Fragments arranged by size:
2573 1535 1156 810 125
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6199
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3151 3240 3444
Size: 416 53 83 399 24 1734 89 204
Cuts at: 3444 3930 4888 442
Size: 486 958 1753
Fragments arranged by size:
1753 1734 958 486 416 399 204 89
83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6199
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2232 2232
Size: 6199
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2408 2516 2780 4859 1239
Size: 1169 108 264 2079 2579
Fragments arranged by size:
2579 2079 1169 264 108
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2324 2501 4324 5189 5487 2324
Size: 177 1823 865 298 3036
Fragments arranged by size:
3036 1823 865 298 177
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2444 4862 882
Size: 617 945 2418 2219
Fragments arranged by size:
2418 2219 945 617
BsaAI yAC'GTr
Cuts at: 493 1204 2224 3990 493
Size: 711 1020 1766 2702
Fragments arranged by size:
2702 1766 1020 711
BsaBI GATnn'nnATC
Cuts at: 2871 6193 2871
Size: 3322 2877
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4006 4388 5023 275
Size: 53 83 186 936 2473 382 635 1451
Fragments arranged by size:
2473 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2106 2230 3059 4632 5030 5304 5451 5862 2106
Size: 124 829 1573 398 274 147 411 2443
Fragments arranged by size:
2443 1573 829 411 398 274 147 124
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5974
Fragments arranged by size:
5974 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2396 1186
Size: 1210 4989
BseYI C'CCAG_C
Cuts at: 3073 5402 5898 3073
Size: 2329 496 3374
Fragments arranged by size:
3374 2329 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2080 2883 1163
Size: 573 344 803 4479
Fragments arranged by size:
4479 803 573 344
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3649 4410 5438 1540
Size: 275 186 1648 761 1028 2301
Fragments arranged by size:
2301 1648 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 4260 4345 5416 4260
Size: 85 1071 5043
Fragments arranged by size:
5043 1071 85
BsmI GAATG_Cn'
Cuts at: 2362 3710 3803 2362
Size: 1348 93 4758
Fragments arranged by size:
4758 1348 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 3325 3450 3518 4260 4345 5416 1391
Size: 399 1535 125 68 742 85 1071 2174
Fragments arranged by size:
2174 1535 1071 742 399 125 85 68
BspEI T'CCGG_A
Cuts at: 5862 5862
Size: 6199
BspHI T'CATG_A
Cuts at: 4088 5818 4088
Size: 1730 4469
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3554 844
Size: 2710 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 4086 1394
Size: 429 2263 3507
Fragments arranged by size:
3507 2263 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2129 4694 4868 65
Size: 1183 881 2565 174 1396
Fragments arranged by size:
2565 1396 1183 881 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4843 5030 1700
Size: 110 3033 187 2869
Fragments arranged by size:
3033 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6199
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6199
BssSI C'ACGA_G
Cuts at: 4259 5271 4259
Size: 1012 5187
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3118 794
Size: 2324 3875
BstBI TT'CG_AA
Cuts at: 5003 5003
Size: 6199
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6199
BstYI r'GATC_y
Cuts at: 1972 2562 3529 3896 4281 4298 5750 5836 5848
Size: 590 967 367 385 17 1452 86 12
Cuts at: 5848 6194 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 967 590 385 367 346 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 3406 513
Size: 552 2341 3306
Fragments arranged by size:
3306 2341 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5460
Fragments arranged by size:
5460 683 56
BtsI GCAGTG_nn'
Cuts at: 3715 4509 4529 3715
Size: 794 20 5385
Fragments arranged by size:
5385 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6199
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6164
DraI TTT'AAA
Cuts at: 3219 3503 3219
Size: 284 5915
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5206 809
Size: 507 3890 1802
Fragments arranged by size:
3890 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3646 4535 6164
Size: 54 1425 50 275 18 1816 889 1629
Cuts at: 6164 8
Size: 43
Fragments arranged by size:
1816 1629 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3646 1537
Size: 275 1834 4090
Fragments arranged by size:
4090 1834 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2245 2619 2640 2835 4129 1353
Size: 892 374 21 195 1294 3423
Fragments arranged by size:
3423 1294 892 374 195 21
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4786 5160 5306 387
Size: 1064 138 3197 374 146 1280
Fragments arranged by size:
3197 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2408 2516 2729 2780 4262 4859 5646
Size: 393 776 108 213 51 1482 597 787
Cuts at: 5646 5914 1239
Size: 268 1524
Fragments arranged by size:
1524 1482 787 776 597 393 268 213
108 51
EcoO109I rG'GnC_Cy
Cuts at: 3446 3446
Size: 6199
EcoRI G'AATT_C
Cuts at: 2500 3508 3886 2500
Size: 1008 378 4813
Fragments arranged by size:
4813 1008 378
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2675 2675
Size: 6199
FspI TGC'GCA
Cuts at: 4705 4705
Size: 6199
HaeII r_GCGC'y
Cuts at: 1678 2074 5346 1678
Size: 396 3272 2531
Fragments arranged by size:
3272 2531 396
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4840 4872 4914 4946 1373
Size: 32 135 32 3268 32 42 32 2626
Fragments arranged by size:
3268 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3333 3543 3789 669
Size: 988 281 1395 210 246 3079
Fragments arranged by size:
3079 1395 988 281 246 210
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6199
HpaI GTT'AAC
Cuts at: 3333 3789 3333
Size: 456 5743
KpnI G_GTAC'C
Cuts at: 3524 3524
Size: 6199
MfeI C'AATT_G
Cuts at: 3798 3798
Size: 6199
MluI A'CGCG_T
Cuts at: 6075 6075
Size: 6199
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2313 2610 2954 5313 5497 1305
Size: 1008 297 344 2359 184 2007
Fragments arranged by size:
2359 2007 1008 344 297 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4377
Fragments arranged by size:
4377 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3473 4157 4516 4675 518
Size: 648 2307 684 359 159 2042
Fragments arranged by size:
2307 2042 684 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2491 3615 4292 5440 5685 1606
Size: 885 1124 677 1148 245 2120
Fragments arranged by size:
2120 1148 1124 885 677 245
NcoI C'CATG_G
Cuts at: 513 1065 3406 513
Size: 552 2341 3306
Fragments arranged by size:
3306 2341 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6199
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6199
NotI GC'GGCC_GC
Cuts at: 3646 3646
Size: 6199
NspI r_CATG'y
Cuts at: 1132 1229 2270 3121 3557 5102 1132
Size: 97 1041 851 436 1545 2229
Fragments arranged by size:
2229 1545 1041 851 436 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6199
PciI A'CATG_T
Cuts at: 2266 5098 2266
Size: 2832 3367
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2701 1108
Size: 1593 4606
PfoI T'CCnGG_A
Cuts at: 2694 2694
Size: 6199
PmeI GTTT'AAAC
Cuts at: 3503 3503
Size: 6199
PmlI CAC'GTG
Cuts at: 2224 2224
Size: 6199
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4268 4300 5781 5813 4268
Size: 32 1481 32 4654
Fragments arranged by size:
4654 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6199
PsiI TTA'TAA
Cuts at: 3769 3769
Size: 6199
PspOMI G'GGCC_C
Cuts at: 3446 3446
Size: 6199
PstI C_TGCA'G
Cuts at: 830 2208 2422 3551 830
Size: 1378 214 1129 3478
Fragments arranged by size:
3478 1378 1129 214
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6199
PvuII CAG'CTG
Cuts at: 2491 2491
Size: 6199
SalI G'TCGA_C
Cuts at: 1936 3541 1936
Size: 1605 4594
SbfI CC_TGCA'GG
Cuts at: 3551 3551
Size: 6199
ScaI AGT'ACT
Cuts at: 4447 6159 4447
Size: 1712 4487
SfcI C'TryA_G
Cuts at: 826 1045 2204 2418 3205 3547 4682 5363 5554
Size: 219 1159 214 787 342 1135 681 191
Cuts at: 5554 826
Size: 1471
Fragments arranged by size:
1471 1159 1135 787 681 342 219 214
191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6199
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6199
SmaI CCC'GGG
Cuts at: 1872 3526 1872
Size: 1654 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2303 2480 4303 5204 5466 820
Size: 197 926 360 177 1823 901 262 1553
Fragments arranged by size:
1823 1553 926 901 360 262 197 177
SnaBI TAC'GTA
Cuts at: 493 3990 493
Size: 3497 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6199
SphI G_CATG'C
Cuts at: 1132 3557 1132
Size: 2425 3774
SspI AAT'ATT
Cuts at: 5 52 3068 4123 5
Size: 47 3016 1055 2081
Fragments arranged by size:
3016 2081 1055 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6199
StyI C'CwwG_G
Cuts at: 513 1065 3406 3638 513
Size: 552 2341 232 3074
Fragments arranged by size:
3074 2341 552 232
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4226 4243 4396 4581 5026 325
Size: 1255 2646 17 153 185 445 1498
Fragments arranged by size:
2646 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2187 3114 4445
Size: 274 80 33 51 1443 210 927 1331
Cuts at: 4445 6157 96
Size: 1712 138
Fragments arranged by size:
1712 1443 1331 927 274 210 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6159 6191 6159
Size: 32 6167
Tth111I GACn'n_nGTC
Cuts at: 1316 2774 1316
Size: 1458 4741
XbaI T'CTAG_A
Cuts at: 3007 3535 3007
Size: 528 5671
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6199
XmaI C'CCGG_G
Cuts at: 1870 3524 1870
Size: 1654 4545
XmnI GAAnn'nnTTC
Cuts at: 4328 4328
Size: 6199
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4007 276
Size: 53 83 186 936 2473 2468
Fragments arranged by size:
2473 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NcoI NdeI NheI NotI NspI PasI PciI
PflMI PfoI PmeI PmlI PpiI PshAI PsiI PspOMI
PstI PvuI PvuII SalI SbfI ScaI SfcI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BbeI BbvCI
BfrBI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII
EcoICRI EcoNI EcoRV FseI FspAI KasI NaeI NarI
NgoMIV NruI NsiI PacI PpuMI PspXI PsrI RsrII
SacI SacII SanDI SapI SexAI SfiI SfoI SrfI
SwaI XcmI