Restriction map for
pFN21AA0002
(Circular) (Six-base) MAPSORT of: pFN21AA0002.seq Check: 3047 from: 1 to: 6349
pFN21AA0002 6349 bp
With 182 enzymes: SgfI *
August 16, 2010 14:41 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4159 278
Size: 53 83 186 936 2623 2468
Fragments arranged by size:
2623 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3180 3692 1937
Size: 1243 512 4594
Fragments arranged by size:
4594 1243 512
Acc65I G'GTAC_C
Cuts at: 3670 3670
Size: 6349
AclI AA'CG_TT
Cuts at: 1563 3435 4476 4849 1563
Size: 1872 1041 373 3063
Fragments arranged by size:
3063 1872 1041 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2370 2634 2811 3477 4412 5796 6064 1632
Size: 738 264 177 666 935 1384 268 1917
Fragments arranged by size:
1917 1384 935 738 666 268 264 177
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6152
AflIII A'CryG_T
Cuts at: 1203 3290 5248 6225 1203
Size: 2087 1958 977 1327
Fragments arranged by size:
2087 1958 1327 977
AgeI A'CCGG_T
Cuts at: 5180 5180
Size: 6349
AhdI GACnn_n'nnGTC
Cuts at: 5078 5078
Size: 6349
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6349
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2265 1184
Size: 1081 5268
AlwNI CAG_nnn'CTG
Cuts at: 3554 4092 5664 3554
Size: 538 1572 4239
Fragments arranged by size:
4239 1572 538
ApaI G_GGCC'C
Cuts at: 3603 3603
Size: 6349
ApaLI G'TGCA_C
Cuts at: 4406 5562 4406
Size: 1156 5193
ApoI r'AATT_y
Cuts at: 1493 2622 2803 3658 3884 4036 6145 1493
Size: 1129 181 855 226 152 2109 1697
Fragments arranged by size:
2109 1697 1129 855 226 181 152
AseI AT'TA_AT
Cuts at: 160 1032 4903 160
Size: 872 3871 1606
Fragments arranged by size:
3871 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6349
AvaI C'yCGr_G
Cuts at: 1870 1943 3674 1870
Size: 73 1731 4545
Fragments arranged by size:
4545 1731 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 3066 3099 3066
Size: 33 6316
BamHI G'GATC_C
Cuts at: 3679 4046 3679
Size: 367 5982
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3113 3670 5125 618
Size: 325 217 627 1326 557 1455 1842
Fragments arranged by size:
1842 1455 1326 627 557 325 217
BanII G_rGCy'C
Cuts at: 1391 2335 2633 3603 3668 1391
Size: 944 298 970 65 4072
Fragments arranged by size:
4072 970 944 298 65
BbeI G_GCGC'C
Cuts at: 3117 3117
Size: 6349
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2977 928
Size: 971 1078 4300
Fragments arranged by size:
4300 1078 971
BbvCI CC'TCA_GC
Cuts at: 3330 3330
Size: 6349
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4540 4574 1903
Size: 34 2603 34 3678
Fragments arranged by size:
3678 2603 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4241 5451 4241
Size: 1210 5139
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6349
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4960 136
Size: 107 122 71 631 605 3288 1525
Fragments arranged by size:
3288 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6344 6344
Size: 6349
BlpI GC'TnA_GC
Cuts at: 3766 3766
Size: 6349
Bme1580I G_kGCm'C
Cuts at: 1790 3603 4410 5566 1790
Size: 1813 807 1156 2573
Fragments arranged by size:
2573 1813 1156 807
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6349
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3637 4080 5038
Size: 416 53 83 399 24 2220 443 958
Cuts at: 5038 442
Size: 1753
Fragments arranged by size:
2220 1753 958 443 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6349
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2349 3393 5009 1239
Size: 1110 1044 1616 2579
Fragments arranged by size:
2579 1616 1110 1044
Bpu10I CC'TnA_GC
Cuts at: 2556 3330 2556
Size: 774 5575
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2033 4474 5339 5637 2033
Size: 2441 865 298 2745
Fragments arranged by size:
2745 2441 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5012 882
Size: 617 3513 2219
Fragments arranged by size:
3513 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 4140 493
Size: 711 2936 2702
Fragments arranged by size:
2936 2702 711
BsaBI GATnn'nnATC
Cuts at: 6343 6343
Size: 6349
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3114 4156 4538 5173
Size: 53 83 186 936 1581 1042 382 635
Cuts at: 5173 275
Size: 1451
Fragments arranged by size:
1581 1451 1042 936 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 4782 5180 5454 5601 6012 4782
Size: 398 274 147 411 5119
Fragments arranged by size:
5119 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2177 2207 1178
Size: 30 165 30 774 30 5320
Fragments arranged by size:
5320 774 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 6349
BseYI C'CCAG_C
Cuts at: 5552 6048 5552
Size: 496 5853
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5776
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3799 4560 5588 1540
Size: 275 186 1798 761 1028 2301
Fragments arranged by size:
2301 1798 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2335 4410 4495 5566 2335
Size: 2075 85 1071 3118
Fragments arranged by size:
3118 2075 1071 85
BsmI GAATG_Cn'
Cuts at: 3860 3953 3860
Size: 93 6256
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2335 2633 3603 3668 4410 4495 5566
Size: 399 545 298 970 65 742 85 1071
Cuts at: 5566 1391
Size: 2174
Fragments arranged by size:
2174 1071 970 742 545 399 298 85
65
BspEI T'CCGG_A
Cuts at: 6012 6012
Size: 6349
BspHI T'CATG_A
Cuts at: 2422 4238 5968 2422
Size: 1816 1730 2803
Fragments arranged by size:
2803 1816 1730
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3704 844
Size: 2860 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 4236 1394
Size: 429 2413 3507
Fragments arranged by size:
3507 2413 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4844 5018 65
Size: 1183 3596 174 1396
Fragments arranged by size:
3596 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4993 5180 1700
Size: 110 3183 187 2869
Fragments arranged by size:
3183 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6349
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6349
BssSI C'ACGA_G
Cuts at: 4409 5421 4409
Size: 1012 5337
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6349
BstBI TT'CG_AA
Cuts at: 5153 5153
Size: 6349
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6349
BstYI r'GATC_y
Cuts at: 1972 2962 3679 4046 4431 4448 5900 5986 5998
Size: 990 717 367 385 17 1452 86 12
Cuts at: 5998 6344 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 990 717 385 367 346 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4858
Fragments arranged by size:
4858 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5610
Fragments arranged by size:
5610 683 56
BtsI GCAGTG_nn'
Cuts at: 2730 3865 4659 4679 2730
Size: 1135 794 20 4400
Fragments arranged by size:
4400 1135 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6349
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6314
DraI TTT'AAA
Cuts at: 3653 3653
Size: 6349
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5356 809
Size: 507 4040 1802
Fragments arranged by size:
4040 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2541 2587 3796
Size: 54 1425 50 275 18 711 46 1209
Cuts at: 3796 4685 6314 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 1209 889 711 275 54 50
46 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3796 1537
Size: 275 1984 4090
Fragments arranged by size:
4090 1984 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2076 2653 2913 3006 4279 1353
Size: 723 577 260 93 1273 3423
Fragments arranged by size:
3423 1273 723 577 260 93
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4936 5310 5456 387
Size: 1064 138 3347 374 146 1280
Fragments arranged by size:
3347 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2333 2333
Size: 6349
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2349 2370 2634 2811 3393 3477 4412
Size: 393 717 21 264 177 582 84 935
Cuts at: 4412 5009 5796 6064 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 935 787 717 597 582 393 268
264 177 84 21
EcoRI G'AATT_C
Cuts at: 3658 4036 3658
Size: 378 5971
FspI TGC'GCA
Cuts at: 4855 4855
Size: 6349
HaeII r_GCGC'y
Cuts at: 1678 2012 3117 5496 1678
Size: 334 1105 2379 2531
Fragments arranged by size:
2531 2379 1105 334
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2468 2500 2684 2716 4990
Size: 32 135 32 896 32 184 32 2274
Cuts at: 4990 5022 5064 5096 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 2274 896 184 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3693 3939 669
Size: 988 281 1755 246 3079
Fragments arranged by size:
3079 1755 988 281 246
HindIII A'AGCT_T
Cuts at: 748 2106 2546 748
Size: 1358 440 4551
Fragments arranged by size:
4551 1358 440
HpaI GTT'AAC
Cuts at: 3939 3939
Size: 6349
KasI G'GCGC_C
Cuts at: 3113 3113
Size: 6349
KpnI G_GTAC'C
Cuts at: 3674 3674
Size: 6349
MfeI C'AATT_G
Cuts at: 3948 3948
Size: 6349
MluI A'CGCG_T
Cuts at: 6225 6225
Size: 6349
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2275 3049 3418 5463 5647 1305
Size: 970 774 369 2045 184 2007
Fragments arranged by size:
2045 2007 970 774 369 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2543 10
Size: 54 1425 343 711 3816
Fragments arranged by size:
3816 1425 711 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2595 2939 4307 4666 4825 518
Size: 648 1429 344 1368 359 159 2042
Fragments arranged by size:
2042 1429 1368 648 359 344 159
MspA1I CmG'CkG
Cuts at: 1606 3593 3765 4442 5590 5835 1606
Size: 1987 172 677 1148 245 2120
Fragments arranged by size:
2120 1987 1148 677 245 172
NarI GG'CG_CC
Cuts at: 3114 3114
Size: 6349
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4858
Fragments arranged by size:
4858 939 552
NdeI CA'TA_TG
Cuts at: 387 2143 2277 3280 387
Size: 1756 134 1003 3456
Fragments arranged by size:
3456 1756 1003 134
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6349
NotI GC'GGCC_GC
Cuts at: 3796 3796
Size: 6349
NspI r_CATG'y
Cuts at: 1132 1229 2565 3294 3481 3707 5252 1132
Size: 97 1336 729 187 226 1545 2229
Fragments arranged by size:
2229 1545 1336 729 226 187 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6349
PciI A'CATG_T
Cuts at: 3290 5248 3290
Size: 1958 4391
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3596 1108
Size: 2488 3861
PfoI T'CCnGG_A
Cuts at: 2337 3295 2337
Size: 958 5391
PmeI GTTT'AAAC
Cuts at: 3653 3653
Size: 6349
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4418 4450 5931 5963 4418
Size: 32 1481 32 4804
Fragments arranged by size:
4804 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6349
PsiI TTA'TAA
Cuts at: 3919 3919
Size: 6349
PspOMI G'GGCC_C
Cuts at: 3599 3599
Size: 6349
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 2220 2252 2220
Size: 32 6317
PstI C_TGCA'G
Cuts at: 830 2414 3550 3701 830
Size: 1584 1136 151 3478
Fragments arranged by size:
3478 1584 1136 151
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6349
PvuII CAG'CTG
Cuts at: 3593 3593
Size: 6349
SacI G_AGCT'C
Cuts at: 2335 2335
Size: 6349
SalI G'TCGA_C
Cuts at: 1936 3691 1936
Size: 1755 4594
SapI GCTCTTCn'nnn_
Cuts at: 2913 3006 2913
Size: 93 6256
SbfI CC_TGCA'GG
Cuts at: 3701 3701
Size: 6349
ScaI AGT'ACT
Cuts at: 3132 4597 6309 3132
Size: 1465 1712 3172
Fragments arranged by size:
3172 1712 1465
SfcI C'TryA_G
Cuts at: 826 1045 2410 2994 3144 3546 3697 4832 5513
Size: 219 1365 584 150 402 151 1135 681
Cuts at: 5513 5704 826
Size: 191 1471
Fragments arranged by size:
1471 1365 1135 681 584 402 219 191
151 150
SfoI GGC'GCC
Cuts at: 3115 3115
Size: 6349
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6349
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6349
SmaI CCC'GGG
Cuts at: 1872 3676 1872
Size: 1804 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2048 4453 5354 5616 820
Size: 197 926 105 2405 901 262 1553
Fragments arranged by size:
2405 1553 926 901 262 197 105
SnaBI TAC'GTA
Cuts at: 493 4140 493
Size: 3647 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6349
SphI G_CATG'C
Cuts at: 1132 2565 3707 1132
Size: 1433 1142 3774
Fragments arranged by size:
3774 1433 1142
SspI AAT'ATT
Cuts at: 5 52 3518 4273 5
Size: 47 3466 755 2081
Fragments arranged by size:
3466 2081 755 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6349
StyI C'CwwG_G
Cuts at: 513 1065 2004 2022 3788 513
Size: 552 939 18 1766 3074
Fragments arranged by size:
3074 1766 939 552 18
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4376 4393 4546 4731 5176 325
Size: 1255 2796 17 153 185 445 1498
Fragments arranged by size:
2796 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2236 2727 3130
Size: 274 80 33 51 1443 259 491 403
Cuts at: 3130 3412 3556 4595 6307 96
Size: 282 144 1039 1712 138
Fragments arranged by size:
1712 1443 1039 491 403 282 274 259
144 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3006 3038 6309 6341 3006
Size: 32 3271 32 3014
Fragments arranged by size:
3271 3014 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6349
XbaI T'CTAG_A
Cuts at: 3496 3685 3496
Size: 189 6160
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6349
XmaI C'CCGG_G
Cuts at: 1870 3674 1870
Size: 1804 4545
XmnI GAAnn'nnTTC
Cuts at: 2355 2535 3345 4478 2355
Size: 180 810 1133 4226
Fragments arranged by size:
4226 1133 810 180
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4157 276
Size: 53 83 186 936 2623 2468
Fragments arranged by size:
2623 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BaeI BamHI BanI BanII BbeI BbsI
BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoRI FspI HaeII
Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI
MmeI MscI MslI MspA1I NarI NcoI NdeI NheI
NotI NspI PasI PciI PflMI PfoI PmeI PpiI
PshAI PsiI PspOMI PsrI PstI PvuI PvuII SacI
SalI SapI SbfI ScaI SfcI SfoI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BfrBI BplI BsiWI
BsmBI BstXI BstZ17I Bsu36I DraIII EcoNI EcoO109I EcoRV
FalI FseI FspAI NaeI NgoMIV NruI NsiI PacI
PmlI PpuMI PspXI RsrII SacII SanDI SexAI SfiI
SrfI SwaI XcmI