Result of FASTA (omim) for
pFN21AE6620
FASTA searches a protein or DNA sequence data bank 36.3.4 Apr, 2011
Please cite:
W.R. Pearson & D.J. Lipman PNAS (1988) 85:2444-2448
Query: pF1KE6620, 327 aa
1>>>pF1KE6620 327 - 327 aa - 327 aa
Library: /omim/omim.rfq.tfa
60827320 residues in 85289 sequences
Statistics: Expectation_n fit: rho(ln(x))= 4.9615+/-0.00035; mu= 17.4521+/- 0.022
mean_var=56.0297+/-11.352, 0's: 0 Z-trim(113.7): 8 B-trim: 33 in 1/53
Lambda= 0.171343
statistics sampled from 23168 (23175) to 23168 sequences
Algorithm: FASTA (3.7 Nov 2010) [optimized]
Parameters: BL50 matrix (15:-5), open/ext: -10/-2
ktup: 2, E-join: 1 (0.645), E-opt: 0.2 (0.272), width: 16
Scan time: 5.760
The best scores are: opt bits E(85289)
NP_612422 (OMIM: 613597,613616) 4-hydroxy-2-oxoglu ( 327) 2188 548.9 5.4e-156
NP_001128142 (OMIM: 613597,613616) 4-hydroxy-2-oxo ( 164) 671 173.7 2.3e-43
NP_110396 (OMIM: 611412) N-acetylneuraminate lyase ( 320) 311 84.9 2.5e-16
NP_001186985 (OMIM: 611412) N-acetylneuraminate ly ( 284) 270 74.7 2.6e-13
NP_001186979 (OMIM: 611412) N-acetylneuraminate ly ( 301) 257 71.5 2.5e-12
NP_001186981 (OMIM: 611412) N-acetylneuraminate ly ( 230) 247 69.0 1.1e-11
NP_001186980 (OMIM: 611412) N-acetylneuraminate ly ( 240) 223 63.1 7e-10
>>NP_612422 (OMIM: 613597,613616) 4-hydroxy-2-oxoglutara (327 aa)
initn: 2188 init1: 2188 opt: 2188 Z-score: 2921.5 bits: 548.9 E(85289): 5.4e-156
Smith-Waterman score: 2188; 100.0% identity (100.0% similar) in 327 aa overlap (1-327:1-327)
10 20 30 40 50 60
pF1KE6 MLGPQVWSSVRQGLSRSLSRNVGVWASGEGKKVDIAGIYPPVTTPFTATAEVDYGKLEEN
::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::
NP_612 MLGPQVWSSVRQGLSRSLSRNVGVWASGEGKKVDIAGIYPPVTTPFTATAEVDYGKLEEN
10 20 30 40 50 60
70 80 90 100 110 120
pF1KE6 LHKLGTFPFRGFVVQGSNGEFPFLTSSERLEVVSRVRQAMPKNRLLLAGSGCESTQATVE
::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::
NP_612 LHKLGTFPFRGFVVQGSNGEFPFLTSSERLEVVSRVRQAMPKNRLLLAGSGCESTQATVE
70 80 90 100 110 120
130 140 150 160 170 180
pF1KE6 MTVSMAQVGADAAMVVTPCYYRGRMSSAALIHHYTKVADLSPIPVVLYSVPANTGLDLPV
::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::
NP_612 MTVSMAQVGADAAMVVTPCYYRGRMSSAALIHHYTKVADLSPIPVVLYSVPANTGLDLPV
130 140 150 160 170 180
190 200 210 220 230 240
pF1KE6 DAVVTLSQHPNIVGMKDSGGDVTRIGLIVHKTRKQDFQVLAGSAGFLMASYALGAVGGVC
::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::
NP_612 DAVVTLSQHPNIVGMKDSGGDVTRIGLIVHKTRKQDFQVLAGSAGFLMASYALGAVGGVC
190 200 210 220 230 240
250 260 270 280 290 300
pF1KE6 ALANVLGAQVCQLERLCCTGQWEDAQKLQHRLIEPNAAVTRRFGIPGLKKIMDWFGYYGG
::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::
NP_612 ALANVLGAQVCQLERLCCTGQWEDAQKLQHRLIEPNAAVTRRFGIPGLKKIMDWFGYYGG
250 260 270 280 290 300
310 320
pF1KE6 PCRAPLQELSPAEEEALRMDFTSNGWL
:::::::::::::::::::::::::::
NP_612 PCRAPLQELSPAEEEALRMDFTSNGWL
310 320
>>NP_001128142 (OMIM: 613597,613616) 4-hydroxy-2-oxoglut (164 aa)
initn: 671 init1: 671 opt: 671 Z-score: 899.5 bits: 173.7 E(85289): 2.3e-43
Smith-Waterman score: 806; 50.2% identity (50.2% similar) in 327 aa overlap (1-327:1-164)
10 20 30 40 50 60
pF1KE6 MLGPQVWSSVRQGLSRSLSRNVGVWASGEGKKVDIAGIYPPVTTPFTATAEVDYGKLEEN
::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::
NP_001 MLGPQVWSSVRQGLSRSLSRNVGVWASGEGKKVDIAGIYPPVTTPFTATAEVDYGKLEEN
10 20 30 40 50 60
70 80 90 100 110 120
pF1KE6 LHKLGTFPFRGFVVQGSNGEFPFLTSSERLEVVSRVRQAMPKNRLLLAGSGCESTQATVE
:::::::::::
NP_001 LHKLGTFPFRG-------------------------------------------------
70
130 140 150 160 170 180
pF1KE6 MTVSMAQVGADAAMVVTPCYYRGRMSSAALIHHYTKVADLSPIPVVLYSVPANTGLDLPV
NP_001 ------------------------------------------------------------
190 200 210 220 230 240
pF1KE6 DAVVTLSQHPNIVGMKDSGGDVTRIGLIVHKTRKQDFQVLAGSAGFLMASYALGAVGGVC
::::::
NP_001 ------------------------------------------------------AVGGVC
250 260 270 280 290 300
pF1KE6 ALANVLGAQVCQLERLCCTGQWEDAQKLQHRLIEPNAAVTRRFGIPGLKKIMDWFGYYGG
::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::
NP_001 ALANVLGAQVCQLERLCCTGQWEDAQKLQHRLIEPNAAVTRRFGIPGLKKIMDWFGYYGG
80 90 100 110 120 130
310 320
pF1KE6 PCRAPLQELSPAEEEALRMDFTSNGWL
:::::::::::::::::::::::::::
NP_001 PCRAPLQELSPAEEEALRMDFTSNGWL
140 150 160
>>NP_110396 (OMIM: 611412) N-acetylneuraminate lyase iso (320 aa)
initn: 117 init1: 67 opt: 311 Z-score: 414.1 bits: 84.9 E(85289): 2.5e-16
Smith-Waterman score: 320; 27.5% identity (55.6% similar) in 306 aa overlap (32-323:5-307)
10 20 30 40 50 60
pF1KE6 LGPQVWSSVRQGLSRSLSRNVGVWASGEGKKVDIAGIYPPVTTPFTATAEVDYGKLEENL
: . :. . ::.: ..:.... . . .
NP_110 MAFPKKKLQGLVAATITPMTENGEINFSVIGQYV
10 20 30
70 80 90 100 110
pF1KE6 HKLGTFP-FRGFVVQGSNGEFPFLTSSERLEVVSR-VRQAMPKNRLLLAGSGCESTQATV
: ... :.:..:: :. ::: .:. . : .. : .. : : . .
NP_110 DYLVKEQGVKNIFVNGTTGEGLSLSVSERRQVAEEWVTKGKDKLDQVIIHVGALSLKESQ
40 50 60 70 80 90
120 130 140 150 160 170
pF1KE6 EMTVSMAQVGADAAMVVTPCYYRGRMSSAALIHHYTKVADLSP-IPVVLYSVPANTGLDL
:.. :..:::. :..: . . .. ::. .:: .: .: : .:: ::. .
NP_110 ELAQHAAEIGADGIAVIAPFFLKP-WTKDILINFLKEVAAAAPALPFYYYHIPALTGVKI
100 110 120 130 140 150
180 190 200 210 220 230
pF1KE6 PVDAVVT--LSQHPNIVGMKDSGGDVTRIGLIVHKTRKQDFQVLAGSAGFLMASYALGAV
.. .. :.. :.. :.: : :. .: : ..:.:.: : : :... ..::.
NP_110 RAEELLDGILDKIPTFQGLKFSDTDLLDFGQCVDQNRQQQFAFLFGVDEQLLSALVMGAT
160 170 180 190 200 210
240 250 260 270 280 290
pF1KE6 GGVCALANVLGAQVCQLERLCCTGQWEDAQKLQ---HRLIEPNAAVTRRFGIPGLKKIMD
:.: . : :: .. :. . .. : . : .:.: : .: ::. : ::
NP_110 GAVGSTYNYLGKKTNQMLEAFEQKDFSLALNYQFCIQRFI--NFVVKLGFGVSQTKAIMT
220 230 240 250 260 270
300 310 320
pF1KE6 WF-GYYGGPCRAPLQ----ELSPAEEEALR-MDFTSNGWL
: :: : ::: :.. . : :. .:: :
NP_110 LVSGIPMGPPRLPLQKASREFTDSAEAKLKSLDFLSFTDLKDGNLEAGS
280 290 300 310 320
>>NP_001186985 (OMIM: 611412) N-acetylneuraminate lyase (284 aa)
initn: 67 init1: 67 opt: 270 Z-score: 360.1 bits: 74.7 E(85289): 2.6e-13
Smith-Waterman score: 270; 26.4% identity (58.6% similar) in 227 aa overlap (32-253:5-230)
10 20 30 40 50 60
pF1KE6 LGPQVWSSVRQGLSRSLSRNVGVWASGEGKKVDIAGIYPPVTTPFTATAEVDYGKLEENL
: . :. . ::.: ..:.... . . .
NP_001 MAFPKKKLQGLVAATITPMTENGEINFSVIGQYV
10 20 30
70 80 90 100 110
pF1KE6 HKLGTFP-FRGFVVQGSNGEFPFLTSSERLEVVSR-VRQAMPKNRLLLAGSGCESTQATV
: ... :.:..:: :. ::: .:. . : .. : .. : : . .
NP_001 DYLVKEQGVKNIFVNGTTGEGLSLSVSERRQVAEEWVTKGKDKLDQVIIHVGALSLKESQ
40 50 60 70 80 90
120 130 140 150 160 170
pF1KE6 EMTVSMAQVGADAAMVVTPCYYRGRMSSAALIHHYTKVADLSP-IPVVLYSVPANTGLDL
:.. :..:::. :..: . . .. ::. .:: .: .: : .:: ::. .
NP_001 ELAQHAAEIGADGIAVIAPFFLKP-WTKDILINFLKEVAAAAPALPFYYYHIPALTGVKI
100 110 120 130 140 150
180 190 200 210 220 230
pF1KE6 PVDAVVT--LSQHPNIVGMKDSGGDVTRIGLIVHKTRKQDFQVLAGSAGFLMASYALGAV
.. .. :.. :.. :.: : :. .: : ..:.:.: : : :... ..::.
NP_001 RAEELLDGILDKIPTFQGLKFSDTDLLDFGQCVDQNRQQQFAFLFGVDEQLLSALVMGAT
160 170 180 190 200 210
240 250 260 270 280 290
pF1KE6 GGVCALANVLGAQVCQLERLCCTGQWEDAQKLQHRLIEPNAAVTRRFGIPGLKKIMDWFG
:.: . : :: .. :.
NP_001 GAVGSTYNYLGKKTNQMLEAFEQKDFSLALNYQFCIQRFINFVVKLENSKLKVSKNQRTL
220 230 240 250 260 270
>>NP_001186979 (OMIM: 611412) N-acetylneuraminate lyase (301 aa)
initn: 117 init1: 67 opt: 257 Z-score: 342.4 bits: 71.5 E(85289): 2.5e-12
Smith-Waterman score: 266; 29.3% identity (55.6% similar) in 225 aa overlap (111-323:67-288)
90 100 110 120 130 140
pF1KE6 FPFLTSSERLEVVSRVRQAMPKNRLLLAGSGCESTQATVEMTVSMAQVGADAAMVVTPCY
: : . . :.. :..:::. :..: .
NP_001 GNCFLPVYKASPLTVTRLWAERLDQVIIHVGALSLKESQELAQHAAEIGADGIAVIAPFF
40 50 60 70 80 90
150 160 170 180 190
pF1KE6 YRGRMSSAALIHHYTKVADLSP-IPVVLYSVPANTGLDLPVDAVVT--LSQHPNIVGMKD
. .. ::. .:: .: .: : .:: ::. . .. .. :.. :.. :.:
NP_001 LKP-WTKDILINFLKEVAAAAPALPFYYYHIPALTGVKIRAEELLDGILDKIPTFQGLKF
100 110 120 130 140 150
200 210 220 230 240 250
pF1KE6 SGGDVTRIGLIVHKTRKQDFQVLAGSAGFLMASYALGAVGGVCALANVLGAQVCQLERLC
: :. .: : ..:.:.: : : :... ..::.:.: . : :: .. :. .
NP_001 SDTDLLDFGQCVDQNRQQQFAFLFGVDEQLLSALVMGATGAVGSTYNYLGKKTNQMLEAF
160 170 180 190 200 210
260 270 280 290 300
pF1KE6 CTGQWEDAQKLQ---HRLIEPNAAVTRRFGIPGLKKIMDWF-GYYGGPCRAPLQ----EL
.. : . : .:.: : .: ::. : :: : :: : ::: :.
NP_001 EQKDFSLALNYQFCIQRFI--NFVVKLGFGVSQTKAIMTLVSGIPMGPPRLPLQKASREF
220 230 240 250 260 270
310 320
pF1KE6 SPAEEEALR-MDFTSNGWL
. . : :. .:: :
NP_001 TDSAEAKLKSLDFLSFTDLKDGNLEAGS
280 290 300
>>NP_001186981 (OMIM: 611412) N-acetylneuraminate lyase (230 aa)
initn: 67 init1: 67 opt: 247 Z-score: 330.8 bits: 69.0 E(85289): 1.1e-11
Smith-Waterman score: 247; 26.3% identity (58.7% similar) in 213 aa overlap (32-239:5-216)
10 20 30 40 50 60
pF1KE6 LGPQVWSSVRQGLSRSLSRNVGVWASGEGKKVDIAGIYPPVTTPFTATAEVDYGKLEENL
: . :. . ::.: ..:.... . . .
NP_001 MAFPKKKLQGLVAATITPMTENGEINFSVIGQYV
10 20 30
70 80 90 100 110
pF1KE6 HKLGTFP-FRGFVVQGSNGEFPFLTSSERLEVVSR-VRQAMPKNRLLLAGSGCESTQATV
: ... :.:..:: :. ::: .:. . : .. : .. : : . .
NP_001 DYLVKEQGVKNIFVNGTTGEGLSLSVSERRQVAEEWVTKGKDKLDQVIIHVGALSLKESQ
40 50 60 70 80 90
120 130 140 150 160 170
pF1KE6 EMTVSMAQVGADAAMVVTPCYYRGRMSSAALIHHYTKVADLSP-IPVVLYSVPANTGLDL
:.. :..:::. :..: . . .. ::. .:: .: .: : .:: ::. .
NP_001 ELAQHAAEIGADGIAVIAPFFLKP-WTKDILINFLKEVAAAAPALPFYYYHIPALTGVKI
100 110 120 130 140 150
180 190 200 210 220 230
pF1KE6 PVDAVVT--LSQHPNIVGMKDSGGDVTRIGLIVHKTRKQDFQVLAGSAGFLMASYALGAV
.. .. :.. :.. :.: : :. .: : ..:.:.: : : :... ..::.
NP_001 RAEELLDGILDKIPTFQGLKFSDTDLLDFGQCVDQNRQQQFAFLFGVDEQLLSALVMGAT
160 170 180 190 200 210
240 250 260 270 280 290
pF1KE6 GGVCALANVLGAQVCQLERLCCTGQWEDAQKLQHRLIEPNAAVTRRFGIPGLKKIMDWFG
:.:
NP_001 GAVGSFVSRDLSTLLSN
220 230
>>NP_001186980 (OMIM: 611412) N-acetylneuraminate lyase (240 aa)
initn: 67 init1: 67 opt: 223 Z-score: 298.4 bits: 63.1 E(85289): 7e-10
Smith-Waterman score: 223; 25.4% identity (56.6% similar) in 205 aa overlap (32-231:5-208)
10 20 30 40 50 60
pF1KE6 LGPQVWSSVRQGLSRSLSRNVGVWASGEGKKVDIAGIYPPVTTPFTATAEVDYGKLEENL
: . :. . ::.: ..:.... . . .
NP_001 MAFPKKKLQGLVAATITPMTENGEINFSVIGQYV
10 20 30
70 80 90 100 110
pF1KE6 HKLGTFP-FRGFVVQGSNGEFPFLTSSERLEVVSR-VRQAMPKNRLLLAGSGCESTQATV
: ... :.:..:: :. ::: .:. . : .. : .. : : . .
NP_001 DYLVKEQGVKNIFVNGTTGEGLSLSVSERRQVAEEWVTKGKDKLDQVIIHVGALSLKESQ
40 50 60 70 80 90
120 130 140 150 160 170
pF1KE6 EMTVSMAQVGADAAMVVTPCYYRGRMSSAALIHHYTKVADLSP-IPVVLYSVPANTGLDL
:.. :..:::. :..: . . .. ::. .:: .: .: : .:: ::. .
NP_001 ELAQHAAEIGADGIAVIAPFFLKP-WTKDILINFLKEVAAAAPALPFYYYHIPALTGVKI
100 110 120 130 140 150
180 190 200 210 220 230
pF1KE6 PVDAVVT--LSQHPNIVGMKDSGGDVTRIGLIVHKTRKQDFQVLAGSAGFLMASYALGAV
.. .. :.. :.. :.: : :. .: : ..:.:.: : : : . .
NP_001 RAEELLDGILDKIPTFQGLKFSDTDLLDFGQCVDQNRQQQFAFLFGVDEFCIQRFINFVV
160 170 180 190 200 210
240 250 260 270 280 290
pF1KE6 GGVCALANVLGAQVCQLERLCCTGQWEDAQKLQHRLIEPNAAVTRRFGIPGLKKIMDWFG
NP_001 KLENSKLKVSKNQRTLPLGTTNFPFLH
220 230 240
327 residues in 1 query sequences
60827320 residues in 85289 library sequences
Tcomplib [36.3.4 Apr, 2011] (8 proc)
start: Tue Nov 8 14:50:58 2016 done: Tue Nov 8 14:50:58 2016
Total Scan time: 5.760 Total Display time: -0.020
Function used was FASTA [36.3.4 Apr, 2011]