Result of FASTA (omim) for
pFN21AE5257
FASTA searches a protein or DNA sequence data bank 36.3.4 Apr, 2011
Please cite:
W.R. Pearson & D.J. Lipman PNAS (1988) 85:2444-2448
Query: pF1KE5257, 208 aa
1>>>pF1KE5257 208 - 208 aa - 208 aa
Library: /omim/omim.rfq.tfa
60827320 residues in 85289 sequences
Statistics: Expectation_n fit: rho(ln(x))= 5.0989+/-0.000296; mu= 14.8493+/- 0.018
mean_var=59.9626+/-12.090, 0's: 0 Z-trim(117.7): 81 B-trim: 1042 in 1/49
Lambda= 0.165628
statistics sampled from 29838 (29920) to 29838 sequences
Algorithm: FASTA (3.7 Nov 2010) [optimized]
Parameters: BL50 matrix (15:-5), open/ext: -10/-2
ktup: 2, E-join: 1 (0.735), E-opt: 0.2 (0.351), width: 16
Scan time: 6.280
The best scores are: opt bits E(85289)
NP_004456 (OMIM: 149730,180920,602115) fibroblast ( 208) 1434 350.3 1.3e-96
XP_005248321 (OMIM: 149730,180920,602115) PREDICTE ( 208) 1434 350.3 1.3e-96
NP_002000 (OMIM: 148180) fibroblast growth factor ( 194) 599 150.8 1.4e-36
NP_065688 (OMIM: 605831) fibroblast growth factor ( 170) 571 144.1 1.3e-34
NP_002001 (OMIM: 600921,612961) fibroblast growth ( 208) 422 108.5 7.9e-24
NP_003859 (OMIM: 300827,309630) fibroblast growth ( 207) 413 106.4 3.5e-23
NP_062825 (OMIM: 605558,615721) fibroblast growth ( 211) 401 103.5 2.6e-22
NP_004455 (OMIM: 165190,190330) fibroblast growth ( 268) 363 94.5 1.7e-19
NP_005238 (OMIM: 164950,610706) fibroblast growth ( 239) 338 88.5 9.8e-18
NP_004105 (OMIM: 300070) fibroblast growth factor ( 245) 330 86.6 3.7e-17
NP_378668 (OMIM: 300070) fibroblast growth factor ( 192) 328 86.1 4.2e-17
NP_001287741 (OMIM: 605831) fibroblast growth fact ( 165) 325 85.3 6.1e-17
XP_005247284 (OMIM: 601513,617166) PREDICTED: fibr ( 207) 324 85.1 8.8e-17
NP_001132970 (OMIM: 300070) fibroblast growth fact ( 199) 323 84.9 1e-16
NP_001132974 (OMIM: 300070) fibroblast growth fact ( 226) 323 84.9 1.1e-16
NP_001132973 (OMIM: 300070) fibroblast growth fact ( 226) 323 84.9 1.1e-16
NP_001132972 (OMIM: 300070) fibroblast growth fact ( 255) 323 84.9 1.2e-16
XP_005262456 (OMIM: 300070) PREDICTED: fibroblast ( 255) 323 84.9 1.2e-16
XP_011533298 (OMIM: 600921,612961) PREDICTED: fibr ( 158) 318 83.6 1.9e-16
NP_066360 (OMIM: 601513,617166) fibroblast growth ( 243) 320 84.2 2e-16
XP_006713601 (OMIM: 601513,617166) PREDICTED: fibr ( 178) 317 83.4 2.5e-16
NP_004104 (OMIM: 601513,617166) fibroblast growth ( 181) 317 83.4 2.5e-16
NP_066276 (OMIM: 134921) fibroblast growth factor ( 208) 310 81.8 9e-16
NP_004106 (OMIM: 601515,609307) fibroblast growth ( 247) 290 77.0 2.8e-14
NP_001308862 (OMIM: 601515,609307) fibroblast grow ( 187) 288 76.5 3.1e-14
NP_001308869 (OMIM: 601515,609307) fibroblast grow ( 187) 288 76.5 3.1e-14
NP_001308867 (OMIM: 601515,609307) fibroblast grow ( 187) 288 76.5 3.1e-14
NP_001308865 (OMIM: 601515,609307) fibroblast grow ( 184) 287 76.2 3.6e-14
NP_001308861 (OMIM: 601515,609307) fibroblast grow ( 184) 287 76.2 3.6e-14
NP_001308873 (OMIM: 601515,609307) fibroblast grow ( 184) 287 76.2 3.6e-14
NP_001308870 (OMIM: 601515,609307) fibroblast grow ( 185) 287 76.2 3.7e-14
NP_001308876 (OMIM: 601515,609307) fibroblast grow ( 200) 287 76.3 3.9e-14
NP_001308877 (OMIM: 601515,609307) fibroblast grow ( 213) 287 76.3 4.1e-14
NP_001308874 (OMIM: 601515,609307) fibroblast grow ( 213) 287 76.3 4.1e-14
NP_787125 (OMIM: 601515,609307) fibroblast growth ( 252) 287 76.3 4.8e-14
XP_016861368 (OMIM: 601513,617166) PREDICTED: fibr ( 157) 278 74.1 1.4e-13
NP_001138406 (OMIM: 131220) fibroblast growth fact ( 155) 270 72.1 5.3e-13
NP_001244136 (OMIM: 131220) fibroblast growth fact ( 155) 270 72.1 5.3e-13
NP_001244137 (OMIM: 131220) fibroblast growth fact ( 155) 270 72.1 5.3e-13
NP_001138407 (OMIM: 131220) fibroblast growth fact ( 155) 270 72.1 5.3e-13
NP_001244139 (OMIM: 131220) fibroblast growth fact ( 155) 270 72.1 5.3e-13
NP_000791 (OMIM: 131220) fibroblast growth factor ( 155) 270 72.1 5.3e-13
NP_001244134 (OMIM: 131220) fibroblast growth fact ( 155) 270 72.1 5.3e-13
NP_001138364 (OMIM: 131220) fibroblast growth fact ( 155) 270 72.1 5.3e-13
NP_001244138 (OMIM: 131220) fibroblast growth fact ( 155) 270 72.1 5.3e-13
NP_001290389 (OMIM: 601514) fibroblast growth fact ( 166) 270 72.2 5.6e-13
NP_001998 (OMIM: 164980) fibroblast growth factor ( 206) 270 72.2 6.7e-13
NP_004103 (OMIM: 601514) fibroblast growth factor ( 225) 270 72.2 7.2e-13
NP_001308872 (OMIM: 601515,609307) fibroblast grow ( 163) 261 70.0 2.4e-12
NP_001308860 (OMIM: 601515,609307) fibroblast grow ( 163) 261 70.0 2.4e-12
>>NP_004456 (OMIM: 149730,180920,602115) fibroblast grow (208 aa)
initn: 1434 init1: 1434 opt: 1434 Z-score: 1855.7 bits: 350.3 E(85289): 1.3e-96
Smith-Waterman score: 1434; 100.0% identity (100.0% similar) in 208 aa overlap (1-208:1-208)
10 20 30 40 50 60
pF1KE5 MWKWILTHCASAFPHLPGCCCCCFLLLFLVSSVPVTCQALGQDMVSPEATNSSSSSFSSP
::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::
NP_004 MWKWILTHCASAFPHLPGCCCCCFLLLFLVSSVPVTCQALGQDMVSPEATNSSSSSFSSP
10 20 30 40 50 60
70 80 90 100 110 120
pF1KE5 SSAGRHVRSYNHLQGDVRWRKLFSFTKYFLKIEKNGKVSGTKKENCPYSILEITSVEIGV
::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::
NP_004 SSAGRHVRSYNHLQGDVRWRKLFSFTKYFLKIEKNGKVSGTKKENCPYSILEITSVEIGV
70 80 90 100 110 120
130 140 150 160 170 180
pF1KE5 VAVKAINSNYYLAMNKKGKLYGSKEFNNDCKLKERIEENGYNTYASFNWQHNGRQMYVAL
::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::
NP_004 VAVKAINSNYYLAMNKKGKLYGSKEFNNDCKLKERIEENGYNTYASFNWQHNGRQMYVAL
130 140 150 160 170 180
190 200
pF1KE5 NGKGAPRRGQKTRRKNTSAHFLPMVVHS
::::::::::::::::::::::::::::
NP_004 NGKGAPRRGQKTRRKNTSAHFLPMVVHS
190 200
>>XP_005248321 (OMIM: 149730,180920,602115) PREDICTED: f (208 aa)
initn: 1434 init1: 1434 opt: 1434 Z-score: 1855.7 bits: 350.3 E(85289): 1.3e-96
Smith-Waterman score: 1434; 100.0% identity (100.0% similar) in 208 aa overlap (1-208:1-208)
10 20 30 40 50 60
pF1KE5 MWKWILTHCASAFPHLPGCCCCCFLLLFLVSSVPVTCQALGQDMVSPEATNSSSSSFSSP
::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::
XP_005 MWKWILTHCASAFPHLPGCCCCCFLLLFLVSSVPVTCQALGQDMVSPEATNSSSSSFSSP
10 20 30 40 50 60
70 80 90 100 110 120
pF1KE5 SSAGRHVRSYNHLQGDVRWRKLFSFTKYFLKIEKNGKVSGTKKENCPYSILEITSVEIGV
::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::
XP_005 SSAGRHVRSYNHLQGDVRWRKLFSFTKYFLKIEKNGKVSGTKKENCPYSILEITSVEIGV
70 80 90 100 110 120
130 140 150 160 170 180
pF1KE5 VAVKAINSNYYLAMNKKGKLYGSKEFNNDCKLKERIEENGYNTYASFNWQHNGRQMYVAL
::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::
XP_005 VAVKAINSNYYLAMNKKGKLYGSKEFNNDCKLKERIEENGYNTYASFNWQHNGRQMYVAL
130 140 150 160 170 180
190 200
pF1KE5 NGKGAPRRGQKTRRKNTSAHFLPMVVHS
::::::::::::::::::::::::::::
XP_005 NGKGAPRRGQKTRRKNTSAHFLPMVVHS
190 200
>>NP_002000 (OMIM: 148180) fibroblast growth factor 7 pr (194 aa)
initn: 692 init1: 523 opt: 599 Z-score: 777.9 bits: 150.8 E(85289): 1.4e-36
Smith-Waterman score: 609; 47.3% identity (75.4% similar) in 207 aa overlap (1-206:1-193)
10 20 30 40 50 60
pF1KE5 MWKWILTHCASAFPHLPGCCCCCFLLLFLVSSVPVTCQALGQDMVSPEATNSSSSSFSSP
: ::::: .: : :: .. ::... ..:. :: .:: ... . :::
NP_002 MHKWILTW---ILPTL--LYRSCFHIICLVGTISLACN----DM-TPEQM-ATNVNCSSP
10 20 30 40
70 80 90 100 110
pF1KE5 SSAGRHVRSYNHLQG-DVRWRKLFSFTKYFLKIEKNGKVSGTKKENCPYSILEITSVEIG
::.:::....: :.: :.:: :...:.:.: :::.::.. . :.:.:: .: .:
NP_002 E---RHTRSYDYMEGGDIRVRRLFCRTQWYLRIDKRGKVKGTQEMKNNYNIMEIRTVAVG
50 60 70 80 90 100
120 130 140 150 160 170
pF1KE5 VVAVKAINSNYYLAMNKKGKLYGSKEFNNDCKLKERIEENGYNTYASFNWQHNGRQMYVA
.::.:...:..::::::.::::..:: :.::..:: : :: :::::: .: ::: .:.::
NP_002 IVAIKGVESEFYLAMNKEGKLYAKKECNEDCNFKELILENHYNTYASAKWTHNGGEMFVA
110 120 130 140 150 160
180 190 200
pF1KE5 LNGKGAPRRGQKTRRKNTSAHFLPMVVHS
:: :: : ::.::.... .::::::..
NP_002 LNQKGIPVRGKKTKKEQKTAHFLPMAIT
170 180 190
>>NP_065688 (OMIM: 605831) fibroblast growth factor 22 i (170 aa)
initn: 635 init1: 571 opt: 571 Z-score: 742.6 bits: 144.1 E(85289): 1.3e-34
Smith-Waterman score: 571; 54.5% identity (85.5% similar) in 145 aa overlap (62-206:25-169)
40 50 60 70 80 90
pF1KE5 SVPVTCQALGQDMVSPEATNSSSSSFSSPSSAGRHVRSYNHLQGDVRWRKLFSFTKYFLK
::.: ::: ::.::::::.::: :..::.
NP_065 MRRRLWLGLAWLLLARAPDAAGTPSASRGPRSYPHLEGDVRWRRLFSSTHFFLR
10 20 30 40 50
100 110 120 130 140 150
pF1KE5 IEKNGKVSGTKKENCPYSILEITSVEIGVVAVKAINSNYYLAMNKKGKLYGSKEFNNDCK
.. .:.:.::. .. ::::: ::..:::..::..:..:.:::..:.::::. .. ::.
NP_065 VDPGGRVQGTRWRHGQDSILEIRSVHVGVVVIKAVSSGFYVAMNRRGRLYGSRLYTVDCR
60 70 80 90 100 110
160 170 180 190 200
pF1KE5 LKERIEENGYNTYASFNWQHNGRQMYVALNGKGAPRRGQKTRRKNTSAHFLPMVVHS
..:::::::.::::: :.. :. :..::. .:.:: : .::: . ::::::..:
NP_065 FRERIEENGHNTYASQRWRRRGQPMFLALDRRGGPRPGGRTRRYHLSAHFLPVLVS
120 130 140 150 160 170
>>NP_002001 (OMIM: 600921,612961) fibroblast growth fact (208 aa)
initn: 419 init1: 311 opt: 422 Z-score: 548.8 bits: 108.5 E(85289): 7.9e-24
Smith-Waterman score: 422; 43.0% identity (79.6% similar) in 142 aa overlap (64-203:48-189)
40 50 60 70 80 90
pF1KE5 PVTCQALGQDMVSPEATNSSSSSFSSPSSAGRHVRSYNHLQGDVRWRKLFSFTKYFLKIE
: : . .::.: .: :.:. : . :.:
NP_002 PFGNVPVLPVDSPVLLSDHLGQSEAGGLPRGPAVTDLDHLKGILRRRQLYCRTGFHLEIF
20 30 40 50 60 70
100 110 120 130 140 150
pF1KE5 KNGKVSGTKKENCPYSILEITSVEIGVVAVKAINSNYYLAMNKKGKLYGSKEFNNDCKLK
:: ..::.:.. ..:::. :. .:.:......:. ::.::.::.::::......: ..
NP_002 PNGTIQGTRKDHSRFGILEFISIAVGLVSIRGVDSGLYLGMNEKGELYGSEKLTQECVFR
80 90 100 110 120 130
160 170 180 190 200
pF1KE5 ERIEENGYNTYASFNWQH--NGRQMYVALNGKGAPRRGQKTRRKNTSAHFLPMVVHS
:..::: ::::.: ..: .::..::::: :.::.: .:.:.. .::::
NP_002 EQFEENWYNTYSSNLYKHVDTGRRYYVALNKDGTPREGTRTKRHQKFTHFLPRPVDPDKV
140 150 160 170 180 190
NP_002 PELYKDILSQS
200
>>NP_003859 (OMIM: 300827,309630) fibroblast growth fact (207 aa)
initn: 405 init1: 298 opt: 413 Z-score: 537.2 bits: 106.4 E(85289): 3.5e-23
Smith-Waterman score: 413; 43.4% identity (78.7% similar) in 136 aa overlap (70-203:53-188)
40 50 60 70 80 90
pF1KE5 LGQDMVSPEATNSSSSSFSSPSSAGRHVRSYNHLQGDVRWRKLFSFTKYFLKIEKNGKVS
. ::.: .: :.:. : . :.: :: :
NP_003 GNVPLADSPGFLNERLGQIEGKLQRGSPTDFAHLKGILRRRQLYCRTGFHLEIFPNGTVH
30 40 50 60 70 80
100 110 120 130 140 150
pF1KE5 GTKKENCPYSILEITSVEIGVVAVKAINSNYYLAMNKKGKLYGSKEFNNDCKLKERIEEN
::.... ..:::. :. .:........:. ::.::..:.:::::... .: ..:..:::
NP_003 GTRHDHSRFGILEFISLAVGLISIRGVDSGLYLGMNERGELYGSKKLTRECVFREQFEEN
90 100 110 120 130 140
160 170 180 190 200
pF1KE5 GYNTYASFNWQHNG--RQMYVALNGKGAPRRGQKTRRKNTSAHFLPMVVHS
:::::: ..:. ::.::::: :.::.: .:.:.. .::::
NP_003 WYNTYASTLYKHSDSERQYYVALNKDGSPREGYRTKRHQKFTHFLPRPVDPSKLPSMSRD
150 160 170 180 190 200
NP_003 LFHYR
>>NP_062825 (OMIM: 605558,615721) fibroblast growth fact (211 aa)
initn: 391 init1: 292 opt: 401 Z-score: 521.6 bits: 103.5 E(85289): 2.6e-22
Smith-Waterman score: 401; 37.4% identity (71.3% similar) in 174 aa overlap (38-203:19-192)
10 20 30 40 50 60
pF1KE5 HCASAFPHLPGCCCCCFLLLFLVSSVPVTCQALGQDMVSPEATNSSSSSFSSPSSAGRHV
: .:. .. : : . :.: : .
NP_062 MAPLAEVGGFLGGLEGLGQQVGSHFLLPPAGERPPLLGERRSAAERSA
10 20 30 40
70 80 90 100 110 120
pF1KE5 RS------YNHLQGDVRWRKLFSFTKYFLKIEKNGKVSGTKKENCPYSILEITSVEIGVV
:. ::.: .: :.:. : . :.: .:.:.::.... ..:::. :: .:.:
NP_062 RGGPGAAQLAHLHGILRRRQLYCRTGFHLQILPDGSVQGTRQDHSLFGILEFISVAVGLV
50 60 70 80 90 100
130 140 150 160 170
pF1KE5 AVKAINSNYYLAMNKKGKLYGSKEFNNDCKLKERIEENGYNTYASFNWQHN--GRQMYVA
......:. ::.:: ::.::::......: ..:..::: ::::.: ..:. ::...::
NP_062 SIRGVDSGLYLGMNDKGELYGSEKLTSECIFREQFEENWYNTYSSNIYKHGDTGRRYFVA
110 120 130 140 150 160
180 190 200
pF1KE5 LNGKGAPRRGQKTRRKNTSAHFLPMVVHS
:: :.:: : ...:.. .::::
NP_062 LNKDGTPRDGARSKRHQKFTHFLPRPVDPERVPELYKDLLMYT
170 180 190 200 210
>>NP_004455 (OMIM: 165190,190330) fibroblast growth fact (268 aa)
initn: 356 init1: 226 opt: 363 Z-score: 471.0 bits: 94.5 E(85289): 1.7e-19
Smith-Waterman score: 363; 39.5% identity (70.3% similar) in 172 aa overlap (38-203:48-217)
10 20 30 40 50 60
pF1KE5 HCASAFPHLPGCCCCCFLLLFLVSSVPVTCQALGQDMVSPEATNSSSSSFSSPSSAGRHV
:. .. : : :..: ..:..: .: : .
NP_004 AWAHGEKRLAPKGQPGPAATDRNPRGSSSRQSSSSAMSSSSASSSPAASLGSQGS-GLEQ
20 30 40 50 60 70
70 80 90 100 110 120
pF1KE5 RSYNHLQGDVRWRKLFSFTK--YFLKIEKNGKVSGTKKENCPYSILEITSVEIGVVAVKA
:.. . : .:. . . :.: .:::.:... : :.::: .: :.:....
NP_004 SSFQWSPSGRRTGSLYCRVGIGFHLQIYPDGKVNGSHEANM-LSVLEIFAVSQGIVGIRG
80 90 100 110 120 130
130 140 150 160 170 180
pF1KE5 INSNYYLAMNKKGKLYGSKEFNNDCKLKERIEENGYNTYAS--FNWQHNGRQMYVALNGK
. :: .:::.:::::..: .:..:::..::..::.:::::: ...::. ::::: .
NP_004 VFSNKFLAMSKKGKLHASAKFTDDCKFRERFQENSYNTYASAIHRTEKTGREWYVALNKR
140 150 160 170 180 190
190 200
pF1KE5 GAPRRGQKTRRK--NTSAHFLPMVVHS
: .:: . : : . :.::::
NP_004 GKAKRGCSPRVKPQHISTHFLPRFKQSEQPELSFTVTVPEKKKPPSPIKPKIPLSAPRKN
200 210 220 230 240 250
>>NP_005238 (OMIM: 164950,610706) fibroblast growth fact (239 aa)
initn: 448 init1: 252 opt: 338 Z-score: 439.4 bits: 88.5 E(85289): 9.8e-18
Smith-Waterman score: 422; 47.4% identity (71.2% similar) in 156 aa overlap (63-206:29-183)
40 50 60 70 80 90
pF1KE5 VPVTCQALGQDMVSPEATNSSSSSFSSPSSAGRHVRSYNHLQGDVRWRKLFSFTKYFLKI
:: . :.:: : : :::. ::: :..
NP_005 MGLIWLLLLSLLEPGWPAAGPGARLRRDAGGRGGVYEHLGGAPRRRKLYCATKYHLQL
10 20 30 40 50
100 110 120 130 140 150
pF1KE5 EKNGKVSGTKKENCPYSILEITSVEIGVVAVKAINSNYYLAMNKKGKLYGSKEFNNDCKL
. .:.:.:. :: :::::::.::.:.::.... :. ::::::.:.::.:.... .:..
NP_005 HPSGRVNGSL-ENSAYSILEITAVEVGIVAIRGLFSGRYLAMNKRGRLYASEHYSAECEF
60 70 80 90 100 110
160 170 180 190 200
pF1KE5 KERIEENGYNTYASFNWQHNG------------RQMYVALNGKGAPRRGQKTRRKNTSAH
:::.: ::::::: .. . : ::..:::: :::: :::: . :.
NP_005 VERIHELGYNTYASRLYRTVSSTPGARRQPSAERLWYVSVNGKGRPRRGFKTRRTQKSSL
120 130 140 150 160 170
pF1KE5 FLPMVVHS
::: :.
NP_005 FLPRVLDHRDHEMVRQLQSGLPRPPGKGVQPRRRRQKQSPDNLEPSHVQASRLGSQLEAS
180 190 200 210 220 230
>>NP_004105 (OMIM: 300070) fibroblast growth factor 13 i (245 aa)
initn: 257 init1: 236 opt: 330 Z-score: 428.9 bits: 86.6 E(85289): 3.7e-17
Smith-Waterman score: 330; 37.2% identity (70.3% similar) in 145 aa overlap (61-203:54-196)
40 50 60 70 80 90
pF1KE5 SSVPVTCQALGQDMVSPEATNSSSSSFSSPSSAGRHVRSYNHLQGDVRWRKLFSFTKYFL
:. :. : .:.: : ::.: : :
NP_004 ACKCVSSPSKGKTSCDKNKLNVFSRVKLFGSKKRRRRRPEPQLKGIV--TKLYSRQGYHL
30 40 50 60 70 80
100 110 120 130 140 150
pF1KE5 KIEKNGKVSGTKKENCPYSILEITSVEIGVVAVKAINSNYYLAMNKKGKLYGSKEFNNDC
... .: ..::: :. :..... : . :::....... :::::..: :: :. :. .:
NP_004 QLQADGTIDGTKDEDSTYTLFNLIPVGLRVVAIQGVQTKLYLAMNSEGYLYTSELFTPEC
90 100 110 120 130 140
160 170 180 190 200
pF1KE5 KLKERIEENGYNTYAS--FNWQHNGRQMYVALNGKGAPRRGQKTRRKNTSAHFLPMVVHS
:.:: . :: : ::.: . :..:: :..:: .: .:....... .:::::
NP_004 KFKESVFENYYVTYSSMIYRQQQSGRGWYLGLNKEGEIMKGNHVKKNKPAAHFLPKPLKV
150 160 170 180 190 200
NP_004 AMYKEPSLHDLTEFSRSGSGTPTKSRSVSGVLNGGKSMSHNEST
210 220 230 240
208 residues in 1 query sequences
60827320 residues in 85289 library sequences
Tcomplib [36.3.4 Apr, 2011] (8 proc)
start: Mon Nov 7 22:56:48 2016 done: Mon Nov 7 22:56:49 2016
Total Scan time: 6.280 Total Display time: -0.030
Function used was FASTA [36.3.4 Apr, 2011]