Result of FASTA (omim) for
pFN21AB7802
FASTA searches a protein or DNA sequence data bank 36.3.4 Apr, 2011
Please cite:
W.R. Pearson & D.J. Lipman PNAS (1988) 85:2444-2448
Query: pF1KB7802, 590 aa
1>>>pF1KB7802 590 - 590 aa - 590 aa
Library: /omim/omim.rfq.tfa
60827320 residues in 85289 sequences
Statistics: Expectation_n fit: rho(ln(x))= 8.9670+/-0.000326; mu= 4.0505+/- 0.020
mean_var=240.0974+/-48.844, 0's: 0 Z-trim(123.7): 161 B-trim: 835 in 1/61
Lambda= 0.082771
statistics sampled from 43900 (44096) to 43900 sequences
Algorithm: FASTA (3.7 Nov 2010) [optimized]
Parameters: BL50 matrix (15:-5), open/ext: -10/-2
ktup: 2, E-join: 1 (0.797), E-opt: 0.2 (0.517), width: 16
Scan time: 12.220
The best scores are: opt bits E(85289)
NP_002508 (OMIM: 603346) neuronal PAS domain-conta ( 590) 4026 493.6 7.5e-139
XP_011525299 (OMIM: 603346) PREDICTED: neuronal PA ( 591) 4014 492.2 2e-138
XP_011525301 (OMIM: 603346) PREDICTED: neuronal PA ( 580) 2399 299.3 2.3e-80
XP_006723294 (OMIM: 603346) PREDICTED: neuronal PA ( 410) 2361 294.7 4e-79
XP_016882331 (OMIM: 603346) PREDICTED: neuronal PA ( 650) 2361 294.9 5.7e-79
XP_011525304 (OMIM: 603346) PREDICTED: neuronal PA ( 454) 2353 293.8 8.5e-79
XP_011525309 (OMIM: 603346) PREDICTED: neuronal PA ( 411) 2349 293.2 1.1e-78
XP_011525308 (OMIM: 603346) PREDICTED: neuronal PA ( 411) 2349 293.2 1.1e-78
XP_016882332 (OMIM: 603346) PREDICTED: neuronal PA ( 582) 2349 293.4 1.4e-78
XP_016882330 (OMIM: 603346) PREDICTED: neuronal PA ( 651) 2349 293.4 1.5e-78
XP_016882333 (OMIM: 603346) PREDICTED: neuronal PA ( 519) 1673 212.6 2.6e-54
XP_011525303 (OMIM: 603346) PREDICTED: neuronal PA ( 483) 1553 198.3 5.1e-50
NP_001308015 (OMIM: 603346) neuronal PAS domain-co ( 234) 1186 154.2 4.6e-37
XP_016877075 (OMIM: 609430) PREDICTED: neuronal PA ( 831) 579 82.1 7.8e-15
XP_016877076 (OMIM: 609430) PREDICTED: neuronal PA ( 831) 579 82.1 7.8e-15
XP_016877077 (OMIM: 609430) PREDICTED: neuronal PA ( 831) 579 82.1 7.8e-15
NP_071406 (OMIM: 609430) neuronal PAS domain-conta ( 901) 579 82.2 8.3e-15
NP_001159365 (OMIM: 609430) neuronal PAS domain-co ( 903) 579 82.2 8.3e-15
XP_011535372 (OMIM: 609430) PREDICTED: neuronal PA ( 918) 579 82.2 8.4e-15
NP_775182 (OMIM: 609430) neuronal PAS domain-conta ( 920) 579 82.2 8.4e-15
NP_001158221 (OMIM: 609430) neuronal PAS domain-co ( 933) 579 82.2 8.5e-15
XP_016877074 (OMIM: 609430) PREDICTED: neuronal PA ( 939) 579 82.2 8.6e-15
XP_011535369 (OMIM: 609430) PREDICTED: neuronal PA ( 950) 579 82.2 8.6e-15
XP_016877073 (OMIM: 609430) PREDICTED: neuronal PA ( 956) 579 82.2 8.7e-15
XP_005268049 (OMIM: 609430) PREDICTED: neuronal PA ( 967) 579 82.2 8.7e-15
XP_005268048 (OMIM: 609430) PREDICTED: neuronal PA ( 969) 579 82.2 8.8e-15
XP_016877071 (OMIM: 609430) PREDICTED: neuronal PA ( 986) 579 82.2 8.9e-15
XP_011535374 (OMIM: 609430) PREDICTED: neuronal PA ( 900) 573 81.5 1.4e-14
XP_011535373 (OMIM: 609430) PREDICTED: neuronal PA ( 936) 573 81.5 1.4e-14
XP_011535371 (OMIM: 609430) PREDICTED: neuronal PA ( 966) 573 81.5 1.4e-14
XP_016877072 (OMIM: 609430) PREDICTED: neuronal PA ( 983) 573 81.5 1.5e-14
NP_001612 (OMIM: 600253) aryl hydrocarbon receptor ( 848) 406 61.5 1.3e-08
XP_011534374 (OMIM: 601665,603128) PREDICTED: sing ( 481) 392 59.6 2.7e-08
XP_005267157 (OMIM: 601665,603128) PREDICTED: sing ( 766) 392 59.8 3.9e-08
XP_016866686 (OMIM: 601665,603128) PREDICTED: sing ( 766) 392 59.8 3.9e-08
NP_005059 (OMIM: 601665,603128) single-minded homo ( 766) 392 59.8 3.9e-08
NP_001229341 (OMIM: 606517) aryl hydrocarbon recep ( 701) 370 57.1 2.2e-07
XP_011527996 (OMIM: 600892) PREDICTED: single-mind ( 566) 361 56.0 4e-07
NP_033664 (OMIM: 600892) single-minded homolog 2 s ( 570) 361 56.0 4.1e-07
NP_005060 (OMIM: 600892) single-minded homolog 2 l ( 667) 361 56.0 4.6e-07
XP_016883931 (OMIM: 600892) PREDICTED: single-mind ( 327) 328 51.8 4.1e-06
NP_065782 (OMIM: 606517) aryl hydrocarbon receptor ( 719) 325 51.8 9.5e-06
NP_851397 (OMIM: 603348) hypoxia-inducible factor ( 735) 292 47.8 0.00015
NP_001521 (OMIM: 603348) hypoxia-inducible factor ( 826) 292 47.9 0.00016
NP_001230013 (OMIM: 603348) hypoxia-inducible fact ( 850) 292 47.9 0.00016
NP_690009 (OMIM: 609976) hypoxia-inducible factor ( 450) 281 46.3 0.00026
XP_016882631 (OMIM: 609976) PREDICTED: hypoxia-ind ( 474) 281 46.4 0.00027
XP_005259212 (OMIM: 609976) PREDICTED: hypoxia-ind ( 587) 281 46.4 0.00031
XP_005259213 (OMIM: 609976) PREDICTED: hypoxia-ind ( 587) 281 46.4 0.00031
XP_005259210 (OMIM: 609976) PREDICTED: hypoxia-ind ( 594) 281 46.4 0.00031
>>NP_002508 (OMIM: 603346) neuronal PAS domain-containin (590 aa)
initn: 4026 init1: 4026 opt: 4026 Z-score: 2613.9 bits: 493.6 E(85289): 7.5e-139
Smith-Waterman score: 4026; 100.0% identity (100.0% similar) in 590 aa overlap (1-590:1-590)
10 20 30 40 50 60
pF1KB7 MAAPYPGSGGGSEVKCVGGRGASVPWDFLPGLMVKAPSGPCLQAQRKEKSRNAARSRRGK
::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::
NP_002 MAAPYPGSGGGSEVKCVGGRGASVPWDFLPGLMVKAPSGPCLQAQRKEKSRNAARSRRGK
10 20 30 40 50 60
70 80 90 100 110 120
pF1KB7 ENLEFFELAKLLPLPGAISSQLDKASIVRLSVTYLRLRRFAALGAPPWGLRAAGPPAGLA
::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::
NP_002 ENLEFFELAKLLPLPGAISSQLDKASIVRLSVTYLRLRRFAALGAPPWGLRAAGPPAGLA
70 80 90 100 110 120
130 140 150 160 170 180
pF1KB7 PGRRGPAALVSEVFEQHLGGHILQSLDGFVFALNQEGKFLYISETVSIYLGLSQVEMTGS
::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::
NP_002 PGRRGPAALVSEVFEQHLGGHILQSLDGFVFALNQEGKFLYISETVSIYLGLSQVEMTGS
130 140 150 160 170 180
190 200 210 220 230 240
pF1KB7 SVFDYIHPGDHSEVLEQLGLRTPTPGPPTPPSVSSSSSSSSSLADTPEIEASLTKVPPSS
::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::
NP_002 SVFDYIHPGDHSEVLEQLGLRTPTPGPPTPPSVSSSSSSSSSLADTPEIEASLTKVPPSS
190 200 210 220 230 240
250 260 270 280 290 300
pF1KB7 LVQERSFFVRMKSTLTKRGLHVKASGYKVIHVTGRLRAHALGLVALGHTLPPAPLAELPL
::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::
NP_002 LVQERSFFVRMKSTLTKRGLHVKASGYKVIHVTGRLRAHALGLVALGHTLPPAPLAELPL
250 260 270 280 290 300
310 320 330 340 350 360
pF1KB7 HGHMIVFRLSLGLTILACESRVSDHMDLGPSELVGRSCYQFVHGQDATRIRQSHVDLLDK
::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::
NP_002 HGHMIVFRLSLGLTILACESRVSDHMDLGPSELVGRSCYQFVHGQDATRIRQSHVDLLDK
310 320 330 340 350 360
370 380 390 400 410 420
pF1KB7 GQVMTGYYRWLQRAGGFVWLQSVATVAGSGKSPGEHHVLWVSHVLSQAEGGQTPLDAFQL
::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::
NP_002 GQVMTGYYRWLQRAGGFVWLQSVATVAGSGKSPGEHHVLWVSHVLSQAEGGQTPLDAFQL
370 380 390 400 410 420
430 440 450 460 470 480
pF1KB7 PASVACEEASSPGPEPTEPEPPTEGKQAAPAENEAPQTQGKRIKVEPGPRETKGSEDSGD
::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::
NP_002 PASVACEEASSPGPEPTEPEPPTEGKQAAPAENEAPQTQGKRIKVEPGPRETKGSEDSGD
430 440 450 460 470 480
490 500 510 520 530 540
pF1KB7 EDPSSHPATPRPEFTSVIRAGVLKQDPVRPWGLAPPGDPPPTLLHAGFLPPVVRGLCTPG
::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::
NP_002 EDPSSHPATPRPEFTSVIRAGVLKQDPVRPWGLAPPGDPPPTLLHAGFLPPVVRGLCTPG
490 500 510 520 530 540
550 560 570 580 590
pF1KB7 TIRYGPAELGLVYPHLQRLGPGPALPEAFYPPLGLPYPGPAGTRLPRKGD
::::::::::::::::::::::::::::::::::::::::::::::::::
NP_002 TIRYGPAELGLVYPHLQRLGPGPALPEAFYPPLGLPYPGPAGTRLPRKGD
550 560 570 580 590
>>XP_011525299 (OMIM: 603346) PREDICTED: neuronal PAS do (591 aa)
initn: 2260 init1: 2260 opt: 4014 Z-score: 2606.1 bits: 492.2 E(85289): 2e-138
Smith-Waterman score: 4014; 99.8% identity (99.8% similar) in 591 aa overlap (1-590:1-591)
10 20 30 40 50 60
pF1KB7 MAAPYPGSGGGSEVKCVGGRGASVPWDFLPGLMVKAPSGPCLQAQRKEKSRNAARSRRGK
::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::
XP_011 MAAPYPGSGGGSEVKCVGGRGASVPWDFLPGLMVKAPSGPCLQAQRKEKSRNAARSRRGK
10 20 30 40 50 60
70 80 90 100 110 120
pF1KB7 ENLEFFELAKLLPLPGAISSQLDKASIVRLSVTYLRLRRFAALGAPPWGLRAAGPPAGLA
::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::
XP_011 ENLEFFELAKLLPLPGAISSQLDKASIVRLSVTYLRLRRFAALGAPPWGLRAAGPPAGLA
70 80 90 100 110 120
130 140 150 160 170 180
pF1KB7 PGRRGPAALVSEVFEQHLGGHILQSLDGFVFALNQEGKFLYISETVSIYLGLSQVEMTGS
::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::
XP_011 PGRRGPAALVSEVFEQHLGGHILQSLDGFVFALNQEGKFLYISETVSIYLGLSQVEMTGS
130 140 150 160 170 180
190 200 210 220 230 240
pF1KB7 SVFDYIHPGDHSEVLEQLGLRTPTPGPPTPPSVSSSSSSSSSLADTPEIEASLTKVPPSS
::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::
XP_011 SVFDYIHPGDHSEVLEQLGLRTPTPGPPTPPSVSSSSSSSSSLADTPEIEASLTKVPPSS
190 200 210 220 230 240
250 260 270 280 290
pF1KB7 LVQERSFFVRMKSTLTKRGLHVKASGYK-VIHVTGRLRAHALGLVALGHTLPPAPLAELP
:::::::::::::::::::::::::::: :::::::::::::::::::::::::::::::
XP_011 LVQERSFFVRMKSTLTKRGLHVKASGYKQVIHVTGRLRAHALGLVALGHTLPPAPLAELP
250 260 270 280 290 300
300 310 320 330 340 350
pF1KB7 LHGHMIVFRLSLGLTILACESRVSDHMDLGPSELVGRSCYQFVHGQDATRIRQSHVDLLD
::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::
XP_011 LHGHMIVFRLSLGLTILACESRVSDHMDLGPSELVGRSCYQFVHGQDATRIRQSHVDLLD
310 320 330 340 350 360
360 370 380 390 400 410
pF1KB7 KGQVMTGYYRWLQRAGGFVWLQSVATVAGSGKSPGEHHVLWVSHVLSQAEGGQTPLDAFQ
::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::
XP_011 KGQVMTGYYRWLQRAGGFVWLQSVATVAGSGKSPGEHHVLWVSHVLSQAEGGQTPLDAFQ
370 380 390 400 410 420
420 430 440 450 460 470
pF1KB7 LPASVACEEASSPGPEPTEPEPPTEGKQAAPAENEAPQTQGKRIKVEPGPRETKGSEDSG
::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::
XP_011 LPASVACEEASSPGPEPTEPEPPTEGKQAAPAENEAPQTQGKRIKVEPGPRETKGSEDSG
430 440 450 460 470 480
480 490 500 510 520 530
pF1KB7 DEDPSSHPATPRPEFTSVIRAGVLKQDPVRPWGLAPPGDPPPTLLHAGFLPPVVRGLCTP
::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::
XP_011 DEDPSSHPATPRPEFTSVIRAGVLKQDPVRPWGLAPPGDPPPTLLHAGFLPPVVRGLCTP
490 500 510 520 530 540
540 550 560 570 580 590
pF1KB7 GTIRYGPAELGLVYPHLQRLGPGPALPEAFYPPLGLPYPGPAGTRLPRKGD
:::::::::::::::::::::::::::::::::::::::::::::::::::
XP_011 GTIRYGPAELGLVYPHLQRLGPGPALPEAFYPPLGLPYPGPAGTRLPRKGD
550 560 570 580 590
>>XP_011525301 (OMIM: 603346) PREDICTED: neuronal PAS do (580 aa)
initn: 1807 init1: 1769 opt: 2399 Z-score: 1563.9 bits: 299.3 E(85289): 2.3e-80
Smith-Waterman score: 2423; 71.0% identity (79.2% similar) in 576 aa overlap (1-568:1-550)
10 20 30 40 50 60
pF1KB7 MAAPYPGSGGGSEVKCVGGRGASVPWDFLPGLMVKAPSGPCLQAQRKEKSRNAARSRRGK
::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::
XP_011 MAAPYPGSGGGSEVKCVGGRGASVPWDFLPGLMVKAPSGPCLQAQRKEKSRNAARSRRGK
10 20 30 40 50 60
70 80 90 100 110 120
pF1KB7 ENLEFFELAKLLPLPGAISSQLDKASIVRLSVTYLRLRRFAALGAPPWGLRAAGPPAGLA
::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::
XP_011 ENLEFFELAKLLPLPGAISSQLDKASIVRLSVTYLRLRRFAALGAPPWGLRAAGPPAGLA
70 80 90 100 110 120
130 140 150 160 170 180
pF1KB7 PGRRGPAALVSEVFEQHLGGHILQSLDGFVFALNQEGKFLYISETVSIYLGLSQVEMTGS
::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::
XP_011 PGRRGPAALVSEVFEQHLGGHILQSLDGFVFALNQEGKFLYISETVSIYLGLSQVEMTGS
130 140 150 160 170 180
190 200 210 220 230 240
pF1KB7 SVFDYIHPGDHSEVLEQLGLRTPTPGPPTPPSVSSSSSSSSSLADTPEIEASLTKVPPSS
::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::
XP_011 SVFDYIHPGDHSEVLEQLGLRTPTPGPPTPPSVSSSSSSSSSLADTPEIEASLTKVPPSS
190 200 210 220 230 240
250 260 270 280 290
pF1KB7 LVQERSFFVRMKSTLTKRGLHVKASGYK-VIHVTGRLRAHALGLVALGHTLPPAPLAELP
:::::::::::::::::::::::::::: :::::::::::::::::::::::::::::::
XP_011 LVQERSFFVRMKSTLTKRGLHVKASGYKQVIHVTGRLRAHALGLVALGHTLPPAPLAELP
250 260 270 280 290 300
300 310 320 330 340 350
pF1KB7 LHGHMIVFRLSLGLTILACESRVSDHMDLGPSELVGRSCYQFVHGQDATRIRQSHVDLLD
::::::::::::::::::::::::::::::::::::::::::::::::::::::::: .
XP_011 LHGHMIVFRLSLGLTILACESRVSDHMDLGPSELVGRSCYQFVHGQDATRIRQSHVD--S
310 320 330 340 350
360 370 380 390 400 410
pF1KB7 KGQVMTGYYRWLQRAGGFVWLQSVATVAGSGKSPGEHHVLWVSHVLSQAEGGQT--PLDA
: .: :. . .: : . :.:: ... ..:.: : .
XP_011 KPRVAK--LLWMPSSFQPAW--PVRRHPARGQSPQ----------MGRLRAGDTHRPSAS
360 370 380 390 400
420 430 440 450 460 470
pF1KB7 FQLPASVA-CEEASSPGPEPTEPEP-PTEGKQAAPAENEAPQTQGKRIKVEPGPRETKGS
. :... .::. :. : :.: :. . : : :. . : .: ...: : .:..
XP_011 SSSRAGASDGREAGCPSGERGPPDPGQTHQSGARPEGNQRLRGQWRRGSLQP-PGHTEAR
410 420 430 440 450 460
480 490 500 510 520 530
pF1KB7 EDSGDEDPSSHPAT-PRPEFTSVIRAGVLKQDPVRP--WGLAPPGDPPPTLLHAGFLPPV
::. :. ... :. . :..: :: : : .:. :
XP_011 VHL------CHPGRGPEAGSGAAMGPGASRGPPAHPPARGLPAAGGAGP--VHTRHHPLR
470 480 490 500 510
540 550 560 570 580 590
pF1KB7 VRGLCTPGTIRYGPAELGLVYPHLQRLGPGPALPEAFYPPLGLPYPGPAGTRLPRKGD
:: ::. . : : : .:::::: :
XP_011 PRG-AGPGVPAPAEAGSGPRAPGGLLPAPGPALPGARGHQAAAEGGLRTGRAAGAGPCDN
520 530 540 550 560 570
XP_011 RGPPGQ
580
>>XP_006723294 (OMIM: 603346) PREDICTED: neuronal PAS do (410 aa)
initn: 2361 init1: 2361 opt: 2361 Z-score: 1541.4 bits: 294.7 E(85289): 4e-79
Smith-Waterman score: 2361; 100.0% identity (100.0% similar) in 356 aa overlap (1-356:1-356)
10 20 30 40 50 60
pF1KB7 MAAPYPGSGGGSEVKCVGGRGASVPWDFLPGLMVKAPSGPCLQAQRKEKSRNAARSRRGK
::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::
XP_006 MAAPYPGSGGGSEVKCVGGRGASVPWDFLPGLMVKAPSGPCLQAQRKEKSRNAARSRRGK
10 20 30 40 50 60
70 80 90 100 110 120
pF1KB7 ENLEFFELAKLLPLPGAISSQLDKASIVRLSVTYLRLRRFAALGAPPWGLRAAGPPAGLA
::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::
XP_006 ENLEFFELAKLLPLPGAISSQLDKASIVRLSVTYLRLRRFAALGAPPWGLRAAGPPAGLA
70 80 90 100 110 120
130 140 150 160 170 180
pF1KB7 PGRRGPAALVSEVFEQHLGGHILQSLDGFVFALNQEGKFLYISETVSIYLGLSQVEMTGS
::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::
XP_006 PGRRGPAALVSEVFEQHLGGHILQSLDGFVFALNQEGKFLYISETVSIYLGLSQVEMTGS
130 140 150 160 170 180
190 200 210 220 230 240
pF1KB7 SVFDYIHPGDHSEVLEQLGLRTPTPGPPTPPSVSSSSSSSSSLADTPEIEASLTKVPPSS
::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::
XP_006 SVFDYIHPGDHSEVLEQLGLRTPTPGPPTPPSVSSSSSSSSSLADTPEIEASLTKVPPSS
190 200 210 220 230 240
250 260 270 280 290 300
pF1KB7 LVQERSFFVRMKSTLTKRGLHVKASGYKVIHVTGRLRAHALGLVALGHTLPPAPLAELPL
::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::
XP_006 LVQERSFFVRMKSTLTKRGLHVKASGYKVIHVTGRLRAHALGLVALGHTLPPAPLAELPL
250 260 270 280 290 300
310 320 330 340 350 360
pF1KB7 HGHMIVFRLSLGLTILACESRVSDHMDLGPSELVGRSCYQFVHGQDATRIRQSHVDLLDK
::::::::::::::::::::::::::::::::::::::::::::::::::::::::
XP_006 HGHMIVFRLSLGLTILACESRVSDHMDLGPSELVGRSCYQFVHGQDATRIRQSHVDSCRG
310 320 330 340 350 360
370 380 390 400 410 420
pF1KB7 GQVMTGYYRWLQRAGGFVWLQSVATVAGSGKSPGEHHVLWVSHVLSQAEGGQTPLDAFQL
XP_006 LRVAAVCGHSGWEREEPRGAPCALGQPRAQPSRGWPNSFGCLPASSQRGL
370 380 390 400 410
>>XP_016882331 (OMIM: 603346) PREDICTED: neuronal PAS do (650 aa)
initn: 4012 init1: 2361 opt: 2361 Z-score: 1538.8 bits: 294.9 E(85289): 5.7e-79
Smith-Waterman score: 3686; 90.4% identity (90.4% similar) in 622 aa overlap (1-562:1-622)
10 20 30 40 50 60
pF1KB7 MAAPYPGSGGGSEVKCVGGRGASVPWDFLPGLMVKAPSGPCLQAQRKEKSRNAARSRRGK
::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::
XP_016 MAAPYPGSGGGSEVKCVGGRGASVPWDFLPGLMVKAPSGPCLQAQRKEKSRNAARSRRGK
10 20 30 40 50 60
70 80 90 100 110 120
pF1KB7 ENLEFFELAKLLPLPGAISSQLDKASIVRLSVTYLRLRRFAALGAPPWGLRAAGPPAGLA
::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::
XP_016 ENLEFFELAKLLPLPGAISSQLDKASIVRLSVTYLRLRRFAALGAPPWGLRAAGPPAGLA
70 80 90 100 110 120
130 140 150 160 170 180
pF1KB7 PGRRGPAALVSEVFEQHLGGHILQSLDGFVFALNQEGKFLYISETVSIYLGLSQVEMTGS
::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::
XP_016 PGRRGPAALVSEVFEQHLGGHILQSLDGFVFALNQEGKFLYISETVSIYLGLSQVEMTGS
130 140 150 160 170 180
190 200 210 220 230 240
pF1KB7 SVFDYIHPGDHSEVLEQLGLRTPTPGPPTPPSVSSSSSSSSSLADTPEIEASLTKVPPSS
::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::
XP_016 SVFDYIHPGDHSEVLEQLGLRTPTPGPPTPPSVSSSSSSSSSLADTPEIEASLTKVPPSS
190 200 210 220 230 240
250 260 270 280 290 300
pF1KB7 LVQERSFFVRMKSTLTKRGLHVKASGYKVIHVTGRLRAHALGLVALGHTLPPAPLAELPL
::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::
XP_016 LVQERSFFVRMKSTLTKRGLHVKASGYKVIHVTGRLRAHALGLVALGHTLPPAPLAELPL
250 260 270 280 290 300
310 320 330 340 350
pF1KB7 HGHMIVFRLSLGLTILACESRVSDHMDLGPSELVGRSCYQFVHGQDATRIRQSHVD----
::::::::::::::::::::::::::::::::::::::::::::::::::::::::
XP_016 HGHMIVFRLSLGLTILACESRVSDHMDLGPSELVGRSCYQFVHGQDATRIRQSHVDCETH
310 320 330 340 350 360
360
pF1KB7 --------------------------------------------------------LLDK
::::
XP_016 LHPPSLPTTPQTRASHSLVPGSPSSGLPTVPPAAPTAPGTPFSIGRAARRLSDASGLLDK
370 380 390 400 410 420
370 380 390 400 410 420
pF1KB7 GQVMTGYYRWLQRAGGFVWLQSVATVAGSGKSPGEHHVLWVSHVLSQAEGGQTPLDAFQL
::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::
XP_016 GQVMTGYYRWLQRAGGFVWLQSVATVAGSGKSPGEHHVLWVSHVLSQAEGGQTPLDAFQL
430 440 450 460 470 480
430 440 450 460 470 480
pF1KB7 PASVACEEASSPGPEPTEPEPPTEGKQAAPAENEAPQTQGKRIKVEPGPRETKGSEDSGD
::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::
XP_016 PASVACEEASSPGPEPTEPEPPTEGKQAAPAENEAPQTQGKRIKVEPGPRETKGSEDSGD
490 500 510 520 530 540
490 500 510 520 530 540
pF1KB7 EDPSSHPATPRPEFTSVIRAGVLKQDPVRPWGLAPPGDPPPTLLHAGFLPPVVRGLCTPG
::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::
XP_016 EDPSSHPATPRPEFTSVIRAGVLKQDPVRPWGLAPPGDPPPTLLHAGFLPPVVRGLCTPG
550 560 570 580 590 600
550 560 570 580 590
pF1KB7 TIRYGPAELGLVYPHLQRLGPGPALPEAFYPPLGLPYPGPAGTRLPRKGD
::::::::::::::::::::::
XP_016 TIRYGPAELGLVYPHLQRLGPGPALPEAFYPPLGLPYPGPAGTRLPRKGD
610 620 630 640 650
>>XP_011525304 (OMIM: 603346) PREDICTED: neuronal PAS do (454 aa)
initn: 1818 init1: 1769 opt: 2353 Z-score: 1535.7 bits: 293.8 E(85289): 8.5e-79
Smith-Waterman score: 2360; 83.4% identity (88.1% similar) in 453 aa overlap (1-451:1-437)
10 20 30 40 50 60
pF1KB7 MAAPYPGSGGGSEVKCVGGRGASVPWDFLPGLMVKAPSGPCLQAQRKEKSRNAARSRRGK
::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::
XP_011 MAAPYPGSGGGSEVKCVGGRGASVPWDFLPGLMVKAPSGPCLQAQRKEKSRNAARSRRGK
10 20 30 40 50 60
70 80 90 100 110 120
pF1KB7 ENLEFFELAKLLPLPGAISSQLDKASIVRLSVTYLRLRRFAALGAPPWGLRAAGPPAGLA
::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::
XP_011 ENLEFFELAKLLPLPGAISSQLDKASIVRLSVTYLRLRRFAALGAPPWGLRAAGPPAGLA
70 80 90 100 110 120
130 140 150 160 170 180
pF1KB7 PGRRGPAALVSEVFEQHLGGHILQSLDGFVFALNQEGKFLYISETVSIYLGLSQVEMTGS
::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::
XP_011 PGRRGPAALVSEVFEQHLGGHILQSLDGFVFALNQEGKFLYISETVSIYLGLSQVEMTGS
130 140 150 160 170 180
190 200 210 220 230 240
pF1KB7 SVFDYIHPGDHSEVLEQLGLRTPTPGPPTPPSVSSSSSSSSSLADTPEIEASLTKVPPSS
::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::
XP_011 SVFDYIHPGDHSEVLEQLGLRTPTPGPPTPPSVSSSSSSSSSLADTPEIEASLTKVPPSS
190 200 210 220 230 240
250 260 270 280 290
pF1KB7 LVQERSFFVRMKSTLTKRGLHVKASGYK-VIHVTGRLRAHALGLVALGHTLPPAPLAELP
:::::::::::::::::::::::::::: :::::::::::::::::::::::::::::::
XP_011 LVQERSFFVRMKSTLTKRGLHVKASGYKQVIHVTGRLRAHALGLVALGHTLPPAPLAELP
250 260 270 280 290 300
300 310 320 330 340 350
pF1KB7 LHGHMIVFRLSLGLTILACESRVSDHMDLGPSELVGRSCYQFVHGQDATRIRQSHVDLLD
::::::::::::::::::::::::::::::::::::::::::::::::::::::::: .
XP_011 LHGHMIVFRLSLGLTILACESRVSDHMDLGPSELVGRSCYQFVHGQDATRIRQSHVD--S
310 320 330 340 350
360 370 380 390 400 410
pF1KB7 KGQVMTGYYRWLQRAGGFVWLQSVATVAGSGKSPGEHHVLWVSHVLSQAEGGQTPLDAFQ
: .: . :. . .: : . :.:: :. : . .:.. : .
XP_011 KPRV--AKLLWMPSSFQPAW--PVRRHPARGQSPQ-------SRSLRR-KGSRLPQRRTR
360 370 380 390 400
420 430 440 450 460 470
pF1KB7 LPASVACEEASSPGPEPTEPEPP-TEGKQAAPAENEAPQTQGKRIKVEPGPRETKGSEDS
: : .::. .: .:. : : . . ::
XP_011 PPRPRA--NASKWSPARGKPKAPRTVATRIPPATRPHRGPSSPLSSGQGS
410 420 430 440 450
480 490 500 510 520 530
pF1KB7 GDEDPSSHPATPRPEFTSVIRAGVLKQDPVRPWGLAPPGDPPPTLLHAGFLPPVVRGLCT
>>XP_011525309 (OMIM: 603346) PREDICTED: neuronal PAS do (411 aa)
initn: 1818 init1: 1769 opt: 2349 Z-score: 1533.7 bits: 293.2 E(85289): 1.1e-78
Smith-Waterman score: 2349; 99.7% identity (99.7% similar) in 357 aa overlap (1-356:1-357)
10 20 30 40 50 60
pF1KB7 MAAPYPGSGGGSEVKCVGGRGASVPWDFLPGLMVKAPSGPCLQAQRKEKSRNAARSRRGK
::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::
XP_011 MAAPYPGSGGGSEVKCVGGRGASVPWDFLPGLMVKAPSGPCLQAQRKEKSRNAARSRRGK
10 20 30 40 50 60
70 80 90 100 110 120
pF1KB7 ENLEFFELAKLLPLPGAISSQLDKASIVRLSVTYLRLRRFAALGAPPWGLRAAGPPAGLA
::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::
XP_011 ENLEFFELAKLLPLPGAISSQLDKASIVRLSVTYLRLRRFAALGAPPWGLRAAGPPAGLA
70 80 90 100 110 120
130 140 150 160 170 180
pF1KB7 PGRRGPAALVSEVFEQHLGGHILQSLDGFVFALNQEGKFLYISETVSIYLGLSQVEMTGS
::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::
XP_011 PGRRGPAALVSEVFEQHLGGHILQSLDGFVFALNQEGKFLYISETVSIYLGLSQVEMTGS
130 140 150 160 170 180
190 200 210 220 230 240
pF1KB7 SVFDYIHPGDHSEVLEQLGLRTPTPGPPTPPSVSSSSSSSSSLADTPEIEASLTKVPPSS
::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::
XP_011 SVFDYIHPGDHSEVLEQLGLRTPTPGPPTPPSVSSSSSSSSSLADTPEIEASLTKVPPSS
190 200 210 220 230 240
250 260 270 280 290
pF1KB7 LVQERSFFVRMKSTLTKRGLHVKASGYK-VIHVTGRLRAHALGLVALGHTLPPAPLAELP
:::::::::::::::::::::::::::: :::::::::::::::::::::::::::::::
XP_011 LVQERSFFVRMKSTLTKRGLHVKASGYKQVIHVTGRLRAHALGLVALGHTLPPAPLAELP
250 260 270 280 290 300
300 310 320 330 340 350
pF1KB7 LHGHMIVFRLSLGLTILACESRVSDHMDLGPSELVGRSCYQFVHGQDATRIRQSHVDLLD
:::::::::::::::::::::::::::::::::::::::::::::::::::::::::
XP_011 LHGHMIVFRLSLGLTILACESRVSDHMDLGPSELVGRSCYQFVHGQDATRIRQSHVDSCR
310 320 330 340 350 360
360 370 380 390 400 410
pF1KB7 KGQVMTGYYRWLQRAGGFVWLQSVATVAGSGKSPGEHHVLWVSHVLSQAEGGQTPLDAFQ
XP_011 GLRVAAVCGHSGWEREEPRGAPCALGQPRAQPSRGWPNSFGCLPASSQRGL
370 380 390 400 410
>>XP_011525308 (OMIM: 603346) PREDICTED: neuronal PAS do (411 aa)
initn: 1818 init1: 1769 opt: 2349 Z-score: 1533.7 bits: 293.2 E(85289): 1.1e-78
Smith-Waterman score: 2349; 99.7% identity (99.7% similar) in 357 aa overlap (1-356:1-357)
10 20 30 40 50 60
pF1KB7 MAAPYPGSGGGSEVKCVGGRGASVPWDFLPGLMVKAPSGPCLQAQRKEKSRNAARSRRGK
::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::
XP_011 MAAPYPGSGGGSEVKCVGGRGASVPWDFLPGLMVKAPSGPCLQAQRKEKSRNAARSRRGK
10 20 30 40 50 60
70 80 90 100 110 120
pF1KB7 ENLEFFELAKLLPLPGAISSQLDKASIVRLSVTYLRLRRFAALGAPPWGLRAAGPPAGLA
::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::
XP_011 ENLEFFELAKLLPLPGAISSQLDKASIVRLSVTYLRLRRFAALGAPPWGLRAAGPPAGLA
70 80 90 100 110 120
130 140 150 160 170 180
pF1KB7 PGRRGPAALVSEVFEQHLGGHILQSLDGFVFALNQEGKFLYISETVSIYLGLSQVEMTGS
::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::
XP_011 PGRRGPAALVSEVFEQHLGGHILQSLDGFVFALNQEGKFLYISETVSIYLGLSQVEMTGS
130 140 150 160 170 180
190 200 210 220 230 240
pF1KB7 SVFDYIHPGDHSEVLEQLGLRTPTPGPPTPPSVSSSSSSSSSLADTPEIEASLTKVPPSS
::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::
XP_011 SVFDYIHPGDHSEVLEQLGLRTPTPGPPTPPSVSSSSSSSSSLADTPEIEASLTKVPPSS
190 200 210 220 230 240
250 260 270 280 290
pF1KB7 LVQERSFFVRMKSTLTKRGLHVKASGYK-VIHVTGRLRAHALGLVALGHTLPPAPLAELP
:::::::::::::::::::::::::::: :::::::::::::::::::::::::::::::
XP_011 LVQERSFFVRMKSTLTKRGLHVKASGYKQVIHVTGRLRAHALGLVALGHTLPPAPLAELP
250 260 270 280 290 300
300 310 320 330 340 350
pF1KB7 LHGHMIVFRLSLGLTILACESRVSDHMDLGPSELVGRSCYQFVHGQDATRIRQSHVDLLD
:::::::::::::::::::::::::::::::::::::::::::::::::::::::::
XP_011 LHGHMIVFRLSLGLTILACESRVSDHMDLGPSELVGRSCYQFVHGQDATRIRQSHVDSCR
310 320 330 340 350 360
360 370 380 390 400 410
pF1KB7 KGQVMTGYYRWLQRAGGFVWLQSVATVAGSGKSPGEHHVLWVSHVLSQAEGGQTPLDAFQ
XP_011 GLRVAAVCGHSGWEREEPRGAPCALGQPRAQPSRGWPNSFGCLPASSQRGL
370 380 390 400 410
>>XP_016882332 (OMIM: 603346) PREDICTED: neuronal PAS do (582 aa)
initn: 2367 init1: 1769 opt: 2349 Z-score: 1531.7 bits: 293.4 E(85289): 1.4e-78
Smith-Waterman score: 2810; 77.0% identity (80.3% similar) in 600 aa overlap (1-535:1-579)
10 20 30 40 50 60
pF1KB7 MAAPYPGSGGGSEVKCVGGRGASVPWDFLPGLMVKAPSGPCLQAQRKEKSRNAARSRRGK
::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::
XP_016 MAAPYPGSGGGSEVKCVGGRGASVPWDFLPGLMVKAPSGPCLQAQRKEKSRNAARSRRGK
10 20 30 40 50 60
70 80 90 100 110 120
pF1KB7 ENLEFFELAKLLPLPGAISSQLDKASIVRLSVTYLRLRRFAALGAPPWGLRAAGPPAGLA
::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::
XP_016 ENLEFFELAKLLPLPGAISSQLDKASIVRLSVTYLRLRRFAALGAPPWGLRAAGPPAGLA
70 80 90 100 110 120
130 140 150 160 170 180
pF1KB7 PGRRGPAALVSEVFEQHLGGHILQSLDGFVFALNQEGKFLYISETVSIYLGLSQVEMTGS
::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::
XP_016 PGRRGPAALVSEVFEQHLGGHILQSLDGFVFALNQEGKFLYISETVSIYLGLSQVEMTGS
130 140 150 160 170 180
190 200 210 220 230 240
pF1KB7 SVFDYIHPGDHSEVLEQLGLRTPTPGPPTPPSVSSSSSSSSSLADTPEIEASLTKVPPSS
::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::
XP_016 SVFDYIHPGDHSEVLEQLGLRTPTPGPPTPPSVSSSSSSSSSLADTPEIEASLTKVPPSS
190 200 210 220 230 240
250 260 270 280 290
pF1KB7 LVQERSFFVRMKSTLTKRGLHVKASGYK-VIHVTGRLRAHALGLVALGHTLPPAPLAELP
:::::::::::::::::::::::::::: :::::::::::::::::::::::::::::::
XP_016 LVQERSFFVRMKSTLTKRGLHVKASGYKQVIHVTGRLRAHALGLVALGHTLPPAPLAELP
250 260 270 280 290 300
300 310 320 330 340 350
pF1KB7 LHGHMIVFRLSLGLTILACESRVSDHMDLGPSELVGRSCYQFVHGQDATRIRQSHVD---
:::::::::::::::::::::::::::::::::::::::::::::::::::::::::
XP_016 LHGHMIVFRLSLGLTILACESRVSDHMDLGPSELVGRSCYQFVHGQDATRIRQSHVDCET
310 320 330 340 350 360
pF1KB7 ---------------------------------------------------------LLD
:::
XP_016 HLHPPSLPTTPQTRASHSLVPGSPSSGLPTVPPAAPTAPGTPFSIGRAARRLSDASGLLD
370 380 390 400 410 420
360 370 380 390 400 410
pF1KB7 KGQVMTGYYRWLQRAGGFVWLQSVATVAGSGKSPGEHHVLWVSHVLSQAEGGQTPLDAFQ
::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::
XP_016 KGQVMTGYYRWLQRAGGFVWLQSVATVAGSGKSPGEHHVLWVSHVLSQAEGGQTPLDAFQ
430 440 450 460 470 480
420 430 440 450 460 470
pF1KB7 LPASVACEEASSPGPEPTEPEPPTEGKQAAPAENEAPQTQGKRIKVEPGPRETKGSEDSG
::::::::::::::::::. . ::: .::. : . .... . .:
XP_016 LPASVACEEASSPGPEPTDGK--TEGWG---------HTQALSIFLF----QSRSLRRKG
490 500 510 520
480 490 500 510 520 530
pF1KB7 DEDPSSHPATPRPEFTSVIRAGVLKQDPVRPWGLAP----PGDPPPTLLHAGFLPPVVRG
.. :. . ::: ::.. : .:.: :: :: : : : :. :
XP_016 SRLPQRRTRPPRP------RANASKWSPARGKPKAPRTVATRIPPATRPHRGPSSPLSSG
530 540 550 560 570
540 550 560 570 580 590
pF1KB7 LCTPGTIRYGPAELGLVYPHLQRLGPGPALPEAFYPPLGLPYPGPAGTRLPRKGD
XP_016 QGS
580
>>XP_016882330 (OMIM: 603346) PREDICTED: neuronal PAS do (651 aa)
initn: 3465 init1: 1769 opt: 2349 Z-score: 1531.0 bits: 293.4 E(85289): 1.5e-78
Smith-Waterman score: 3666; 90.2% identity (90.2% similar) in 622 aa overlap (1-561:1-622)
10 20 30 40 50 60
pF1KB7 MAAPYPGSGGGSEVKCVGGRGASVPWDFLPGLMVKAPSGPCLQAQRKEKSRNAARSRRGK
::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::
XP_016 MAAPYPGSGGGSEVKCVGGRGASVPWDFLPGLMVKAPSGPCLQAQRKEKSRNAARSRRGK
10 20 30 40 50 60
70 80 90 100 110 120
pF1KB7 ENLEFFELAKLLPLPGAISSQLDKASIVRLSVTYLRLRRFAALGAPPWGLRAAGPPAGLA
::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::
XP_016 ENLEFFELAKLLPLPGAISSQLDKASIVRLSVTYLRLRRFAALGAPPWGLRAAGPPAGLA
70 80 90 100 110 120
130 140 150 160 170 180
pF1KB7 PGRRGPAALVSEVFEQHLGGHILQSLDGFVFALNQEGKFLYISETVSIYLGLSQVEMTGS
::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::
XP_016 PGRRGPAALVSEVFEQHLGGHILQSLDGFVFALNQEGKFLYISETVSIYLGLSQVEMTGS
130 140 150 160 170 180
190 200 210 220 230 240
pF1KB7 SVFDYIHPGDHSEVLEQLGLRTPTPGPPTPPSVSSSSSSSSSLADTPEIEASLTKVPPSS
::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::
XP_016 SVFDYIHPGDHSEVLEQLGLRTPTPGPPTPPSVSSSSSSSSSLADTPEIEASLTKVPPSS
190 200 210 220 230 240
250 260 270 280 290
pF1KB7 LVQERSFFVRMKSTLTKRGLHVKASGYK-VIHVTGRLRAHALGLVALGHTLPPAPLAELP
:::::::::::::::::::::::::::: :::::::::::::::::::::::::::::::
XP_016 LVQERSFFVRMKSTLTKRGLHVKASGYKQVIHVTGRLRAHALGLVALGHTLPPAPLAELP
250 260 270 280 290 300
300 310 320 330 340 350
pF1KB7 LHGHMIVFRLSLGLTILACESRVSDHMDLGPSELVGRSCYQFVHGQDATRIRQSHVD---
:::::::::::::::::::::::::::::::::::::::::::::::::::::::::
XP_016 LHGHMIVFRLSLGLTILACESRVSDHMDLGPSELVGRSCYQFVHGQDATRIRQSHVDCET
310 320 330 340 350 360
pF1KB7 ---------------------------------------------------------LLD
:::
XP_016 HLHPPSLPTTPQTRASHSLVPGSPSSGLPTVPPAAPTAPGTPFSIGRAARRLSDASGLLD
370 380 390 400 410 420
360 370 380 390 400 410
pF1KB7 KGQVMTGYYRWLQRAGGFVWLQSVATVAGSGKSPGEHHVLWVSHVLSQAEGGQTPLDAFQ
::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::
XP_016 KGQVMTGYYRWLQRAGGFVWLQSVATVAGSGKSPGEHHVLWVSHVLSQAEGGQTPLDAFQ
430 440 450 460 470 480
420 430 440 450 460 470
pF1KB7 LPASVACEEASSPGPEPTEPEPPTEGKQAAPAENEAPQTQGKRIKVEPGPRETKGSEDSG
::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::
XP_016 LPASVACEEASSPGPEPTEPEPPTEGKQAAPAENEAPQTQGKRIKVEPGPRETKGSEDSG
490 500 510 520 530 540
480 490 500 510 520 530
pF1KB7 DEDPSSHPATPRPEFTSVIRAGVLKQDPVRPWGLAPPGDPPPTLLHAGFLPPVVRGLCTP
::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::
XP_016 DEDPSSHPATPRPEFTSVIRAGVLKQDPVRPWGLAPPGDPPPTLLHAGFLPPVVRGLCTP
550 560 570 580 590 600
540 550 560 570 580 590
pF1KB7 GTIRYGPAELGLVYPHLQRLGPGPALPEAFYPPLGLPYPGPAGTRLPRKGD
::::::::::::::::::::::
XP_016 GTIRYGPAELGLVYPHLQRLGPGPALPEAFYPPLGLPYPGPAGTRLPRKGD
610 620 630 640 650
590 residues in 1 query sequences
60827320 residues in 85289 library sequences
Tcomplib [36.3.4 Apr, 2011] (8 proc)
start: Sun Nov 6 04:40:19 2016 done: Sun Nov 6 04:40:21 2016
Total Scan time: 12.220 Total Display time: 0.090
Function used was FASTA [36.3.4 Apr, 2011]