Restriction map for
pFN21AE9690
(Linear) (Six-base) MAPSORT of: KIEE9690.seq Check: 6334 from: 1 to: 2955
KIEE9690.orf
With 182 enzymes: SgfI *
December 5, 2014 13:30 ..
AatII G_ACGT'C
Cuts at: 0 2844 2955
Size: 2844 111
AccI GT'mk_AC
Cuts at: 0 1923 2955
Size: 1923 1032
Acc65I G'GTAC_C
Cuts at: 0 1666 2955
Size: 1666 1289
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 446 833 1262 1304 2955
Size: 446 387 429 42 1651
Fragments arranged by size:
1651 446 429 387 42
AfeI AGC'GCT
Cuts at: 0 1224 2955
Size: 1224 1731
AleI CACnn'nnGTG
Cuts at: 0 200 2955
Size: 200 2755
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1035 1067 2955
Size: 1035 32 1888
Fragments arranged by size:
1888 1035 32
AlwNI CAG_nnn'CTG
Cuts at: 0 571 2229 2955
Size: 571 1658 726
Fragments arranged by size:
1658 726 571
ApaLI G'TGCA_C
Cuts at: 0 533 2955
Size: 533 2422
ApoI r'AATT_y
Cuts at: 0 187 2955
Size: 187 2768
AseI AT'TA_AT
Cuts at: 0 167 697 950 2955
Size: 167 530 253 2005
Fragments arranged by size:
2005 530 253 167
AvaI C'yCGr_G
Cuts at: 0 1787 2955
Size: 1787 1168
AvrII C'CTAG_G
Cuts at: 0 363 2955
Size: 363 2592
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 1166 1199 1698 1731 2955
Size: 1166 33 499 33 1224
Fragments arranged by size:
1224 1166 499 33 33
BamHI G'GATC_C
Cuts at: 0 104 2955
Size: 104 2851
BanI G'GyrC_C
Cuts at: 0 1666 2112 2955
Size: 1666 446 843
Fragments arranged by size:
1666 843 446
BanII G_rGCy'C
Cuts at: 0 32 125 2535 2955
Size: 32 93 2410 420
Fragments arranged by size:
2410 420 93 32
BbsI GAAGACnn'nnnn_
Cuts at: 0 2786 2836 2955
Size: 2786 50 119
Fragments arranged by size:
2786 119 50
BbvCI CC'TCA_GC
Cuts at: 0 781 2159 2955
Size: 781 1378 796
Fragments arranged by size:
1378 796 781
BfrBI ATG'CAT
Cuts at: 0 1486 2955
Size: 1486 1469
BglI GCCn_nnn'nGGC
Cuts at: 0 1125 2955
Size: 1125 1830
BglII A'GATC_T
Cuts at: 0 1033 2955
Size: 1033 1922
Bme1580I G_kGCm'C
Cuts at: 0 537 2301 2911 2955
Size: 537 1764 610 44
Fragments arranged by size:
1764 610 537 44
BmrI ACTGGGnnnn_n'
Cuts at: 0 1573 2434 2955
Size: 1573 861 521
Fragments arranged by size:
1573 861 521
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1281 2955
Size: 1281 1674
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1014 2201 2387 2459 2955
Size: 1014 1187 186 72 496
Fragments arranged by size:
1187 1014 496 186 72
Bpu10I CC'TnA_GC
Cuts at: 0 781 1702 2159 2955
Size: 781 921 457 796
Fragments arranged by size:
921 796 781 457
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1143 1356 2018 2955
Size: 1143 213 662 937
Fragments arranged by size:
1143 937 662 213
BsaI GGTCTCn'nnnn_
Cuts at: 0 545 1013 2955
Size: 545 468 1942
Fragments arranged by size:
1942 545 468
BsaHI Gr'CG_yC
Cuts at: 0 2841 2955
Size: 2841 114
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1035 1065 1395 1425 2010 2040 2199 2229
Size: 1035 30 330 30 585 30 159 30
Cuts at: 2229 2955
Size: 726
Fragments arranged by size:
1035 726 585 330 159 30 30 30
30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 2003 2286 2495 2955
Size: 2003 283 209 460
Fragments arranged by size:
2003 460 283 209
BseYI C'CCAG_C
Cuts at: 0 1445 1569 1883 1889 2936 2955
Size: 1445 124 314 6 1047 19
Fragments arranged by size:
1445 1047 314 124 19 6
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 334 2132 2728 2955
Size: 334 1798 596 227
Fragments arranged by size:
1798 596 334 227
BsiHKAI G_wGCw'C
Cuts at: 0 32 537 2535 2955
Size: 32 505 1998 420
Fragments arranged by size:
1998 505 420 32
BsmI GAATG_Cn'
Cuts at: 0 516 1137 1992 2955
Size: 516 621 855 963
Fragments arranged by size:
963 855 621 516
BsmBI CGTCTCn'nnnn_
Cuts at: 0 2832 2955
Size: 2832 123
Bsp1286I G_dGCh'C
Cuts at: 0 32 125 537 2301 2535 2911 2955
Size: 32 93 412 1764 234 376 44
Fragments arranged by size:
1764 412 376 234 93 44 32
BspHI T'CATG_A
Cuts at: 0 1677 2238 2955
Size: 1677 561 717
Fragments arranged by size:
1677 717 561
BsrDI GCAATG_nn'
Cuts at: 0 1424 1687 2470 2676 2955
Size: 1424 263 783 206 279
Fragments arranged by size:
1424 783 279 263 206
BsrGI T'GTAC_A
Cuts at: 0 2061 2710 2955
Size: 2061 649 245
Fragments arranged by size:
2061 649 245
BstEII G'GTnAC_C
Cuts at: 0 238 1427 1525 2955
Size: 238 1189 98 1430
Fragments arranged by size:
1430 1189 238 98
BstXI CCAn_nnnn'nTGG
Cuts at: 0 298 2955
Size: 298 2657
BstYI r'GATC_y
Cuts at: 0 104 1033 2955
Size: 104 929 1922
Fragments arranged by size:
1922 929 104
Bsu36I CC'TnA_GG
Cuts at: 0 876 2955
Size: 876 2079
BtgI C'CryG_G
Cuts at: 0 1084 2955
Size: 1084 1871
BtsI GCAGTG_nn'
Cuts at: 0 792 2745 2955
Size: 792 1953 210
Fragments arranged by size:
1953 792 210
DraIII CAC_nnn'GTG
Cuts at: 0 376 1459 1645 2467 2871 2955
Size: 376 1083 186 822 404 84
Fragments arranged by size:
1083 822 404 376 186 84
EaeI y'GGCC_r
Cuts at: 0 1072 1803 2955
Size: 1072 731 1152
Fragments arranged by size:
1152 1072 731
EarI CTCTTCn'nnn_
Cuts at: 0 49 801 2540 2955
Size: 49 752 1739 415
Fragments arranged by size:
1739 752 415 49
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 2018 2955
Size: 2018 937
EcoICRI GAG'CTC
Cuts at: 0 30 2533 2955
Size: 30 2503 422
Fragments arranged by size:
2503 422 30
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 446 833 1014 1262 1304 2201 2387 2459
Size: 446 387 181 248 42 897 186 72
Cuts at: 2459 2955
Size: 496
Fragments arranged by size:
897 496 446 387 248 186 181 72
42
EcoNI CCTnn'n_nnAGG
Cuts at: 0 666 2955
Size: 666 2289
EcoO109I rG'GnC_Cy
Cuts at: 0 2617 2955
Size: 2617 338
EcoRI G'AATT_C
Cuts at: 0 187 2955
Size: 187 2768
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 124 1448 1853 2955
Size: 124 1324 405 1102
Fragments arranged by size:
1324 1102 405 124
FspI TGC'GCA
Cuts at: 0 1877 2955
Size: 1877 1078
HaeII r_GCGC'y
Cuts at: 0 1226 2955
Size: 1226 1729
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 553 585 1781 1813 2010 2042 2217 2249
Size: 553 32 1196 32 197 32 175 32
Cuts at: 2249 2271 2303 2590 2622 2955
Size: 22 32 287 32 333
Fragments arranged by size:
1196 553 333 287 197 175 32 32
32 32 32 32 22
HincII GTy'rAC
Cuts at: 0 1834 1924 2880 2955
Size: 1834 90 956 75
Fragments arranged by size:
1834 956 90 75
HindIII A'AGCT_T
Cuts at: 0 911 1976 2955
Size: 911 1065 979
Fragments arranged by size:
1065 979 911
KpnI G_GTAC'C
Cuts at: 0 1670 2955
Size: 1670 1285
MfeI C'AATT_G
Cuts at: 0 2699 2955
Size: 2699 256
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 972 2314 2955
Size: 972 1342 641
Fragments arranged by size:
1342 972 641
MscI TGG'CCA
Cuts at: 0 1805 2955
Size: 1805 1150
MslI CAynn'nnrTG
Cuts at: 0 200 1730 1997 2237 2363 2955
Size: 200 1530 267 240 126 592
Fragments arranged by size:
1530 592 267 240 200 126
MspA1I CmG'CkG
Cuts at: 0 868 1282 1573 1816 2146 2940 2955
Size: 868 414 291 243 330 794 15
Fragments arranged by size:
868 794 414 330 291 243 15
NdeI CA'TA_TG
Cuts at: 0 2914 2955
Size: 2914 41
NsiI A_TGCA'T
Cuts at: 0 1488 2955
Size: 1488 1467
NspI r_CATG'y
Cuts at: 0 1935 2068 2955
Size: 1935 133 887
Fragments arranged by size:
1935 887 133
PasI CC'CwG_GG
Cuts at: 0 2691 2955
Size: 2691 264
PflMI CCAn_nnn'nTGG
Cuts at: 0 2344 2955
Size: 2344 611
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1035 1067 2955
Size: 1035 32 1888
Fragments arranged by size:
1888 1035 32
PpuMI rG'GwC_Cy
Cuts at: 0 2617 2955
Size: 2617 338
PstI C_TGCA'G
Cuts at: 0 153 2451 2955
Size: 153 2298 504
Fragments arranged by size:
2298 504 153
PvuII CAG'CTG
Cuts at: 0 868 1282 1573 2146 2940 2955
Size: 868 414 291 573 794 15
Fragments arranged by size:
868 794 573 414 291 15
SacI G_AGCT'C
Cuts at: 0 32 2535 2955
Size: 32 2503 420
Fragments arranged by size:
2503 420 32
SalI G'TCGA_C
Cuts at: 0 1922 2955
Size: 1922 1033
SapI GCTCTTCn'nnn_
Cuts at: 0 2540 2955
Size: 2540 415
SbfI CC_TGCA'GG
Cuts at: 0 153 2955
Size: 153 2802
SexAI A'CCwGG_T
Cuts at: 0 2463 2955
Size: 2463 492
SfcI C'TryA_G
Cuts at: 0 149 1178 1358 2447 2955
Size: 149 1029 180 1089 508
Fragments arranged by size:
1089 1029 508 180 149
SmlI C'TyrA_G
Cuts at: 0 1122 1371 2033 2955
Size: 1122 249 662 922
Fragments arranged by size:
1122 922 662 249
SspI AAT'ATT
Cuts at: 0 344 2955
Size: 344 2611
StuI AGG'CCT
Cuts at: 0 1264 2955
Size: 1264 1691
StyI C'CwwG_G
Cuts at: 0 363 1020 1423 2955
Size: 363 657 403 1532
Fragments arranged by size:
1532 657 403 363
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 995 1583 2351 2955
Size: 995 588 768 604
Fragments arranged by size:
995 768 604 588
TatI w'GTAC_w
Cuts at: 0 1632 2061 2140 2710 2797 2955
Size: 1632 429 79 570 87 158
Fragments arranged by size:
1632 570 429 158 87 79
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 360 392 2199 2231 2955
Size: 360 32 1807 32 724
Fragments arranged by size:
1807 724 360 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 2379 2955
Size: 2379 576
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 2919 2955
Size: 2919 36
XmnI GAAnn'nnTTC
Cuts at: 0 187 2645 2955
Size: 187 2458 310
Fragments arranged by size:
2458 310 187
ZraI GAC'GTC
Cuts at: 0 2842 2955
Size: 2842 113
Enzymes that do cut:
AatII AccI Acc65I AcuI AfeI AleI AloI AlwNI
ApaLI ApoI AseI AvaI AvrII BaeI BamHI BanI
BanII BbsI BbvCI BfrBI BglI BglII Bme1580I BmrI
BplI BpmI Bpu10I BpuEI BsaI BsaHI BsaXI BseRI
BseYI BsgI BsiHKAI BsmI BsmBI Bsp1286I BspHI BsrDI
BsrGI BstEII BstXI BstYI Bsu36I BtgI BtsI DraIII
EaeI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII HindIII KpnI MfeI
MmeI MscI MslI MspA1I NdeI NsiI NspI PasI
PflMI PpiI PpuMI PstI PvuII SacI SalI SapI
SbfI SexAI SfcI SmlI SspI StuI StyI TaqII
TatI TstI Tth111I XcmI XmnI ZraI
Enzymes that do not cut:
AarI AclI AflII AflIII AgeI AhdI AlfI ApaI
AscI AsiSI BbeI BcgI BciVI BclI BlpI BmgBI
BmtI BsaAI BsaBI BsaWI BsiEI BsiWI BspEI BspMI
BsrBI BsrFI BssHII BssSI BstAPI BstBI BstZ17I BtgZI
ClaI CspCI DraI DrdI EagI EcoRV FseI FspAI
HpaI KasI MluI NaeI NarI NcoI NgoMIV NheI
NotI NruI PacI PciI PfoI PmeI PmlI PshAI
PsiI PspOMI PspXI PsrI PvuI RsrII SacII SanDI
ScaI SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI
SphI SrfI SwaI XbaI XhoI XmaI