Restriction map for
pFN21AE9689
(Linear) (Six-base) MAPSORT of: KIEE9689.seq Check: 7915 from: 1 to: 1470
KIEE9689.orf
With 182 enzymes: SgfI *
December 5, 2014 13:30 ..
AclI AA'CG_TT
Cuts at: 0 812 1470
Size: 812 658
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1189 1339 1470
Size: 1189 150 131
Fragments arranged by size:
1189 150 131
AflII C'TTAA_G
Cuts at: 0 1314 1470
Size: 1314 156
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 530 1470
Size: 530 940
AlwNI CAG_nnn'CTG
Cuts at: 0 754 1470
Size: 754 716
ApaLI G'TGCA_C
Cuts at: 0 1334 1470
Size: 1334 136
ApoI r'AATT_y
Cuts at: 0 3 538 1456 1470
Size: 3 535 918 14
Fragments arranged by size:
918 535 14 3
AvaI C'yCGr_G
Cuts at: 0 395 1470
Size: 395 1075
BanII G_rGCy'C
Cuts at: 0 327 396 1470
Size: 327 69 1074
Fragments arranged by size:
1074 327 69
BbsI GAAGACnn'nnnn_
Cuts at: 0 812 1470
Size: 812 658
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 145 179 1470
Size: 145 34 1291
Fragments arranged by size:
1291 145 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 606 1470
Size: 606 864
BglII A'GATC_T
Cuts at: 0 633 1470
Size: 633 837
BlpI GC'TnA_GC
Cuts at: 0 1160 1470
Size: 1160 310
Bme1580I G_kGCm'C
Cuts at: 0 1338 1470
Size: 1338 132
BmtI G_CTAG'C
Cuts at: 0 772 1470
Size: 772 698
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 227 1470
Size: 227 1243
Bpu10I CC'TnA_GC
Cuts at: 0 658 1470
Size: 658 812
BsaBI GATnn'nnATC
Cuts at: 0 563 1470
Size: 563 907
BsaHI Gr'CG_yC
Cuts at: 0 342 1470
Size: 342 1128
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1034 1470
Size: 1034 436
BsiHKAI G_wGCw'C
Cuts at: 0 327 417 1338 1470
Size: 327 90 921 132
Fragments arranged by size:
921 327 132 90
BsmI GAATG_Cn'
Cuts at: 0 611 1470
Size: 611 859
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1290 1470
Size: 1290 180
Bsp1286I G_dGCh'C
Cuts at: 0 327 396 417 1338 1470
Size: 327 69 21 921 132
Fragments arranged by size:
921 327 132 69 21
BsrDI GCAATG_nn'
Cuts at: 0 114 1298 1470
Size: 114 1184 172
Fragments arranged by size:
1184 172 114
BstYI r'GATC_y
Cuts at: 0 132 282 564 633 1197 1462 1470
Size: 132 150 282 69 564 265 8
Fragments arranged by size:
564 282 265 150 132 69 8
BtgI C'CryG_G
Cuts at: 0 718 1081 1470
Size: 718 363 389
Fragments arranged by size:
718 389 363
DraI TTT'AAA
Cuts at: 0 228 1470
Size: 228 1242
EaeI y'GGCC_r
Cuts at: 0 295 1470
Size: 295 1175
EarI CTCTTCn'nnn_
Cuts at: 0 33 939 1149 1470
Size: 33 906 210 321
Fragments arranged by size:
906 321 210 33
EcoICRI GAG'CTC
Cuts at: 0 325 1470
Size: 325 1145
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 227 1189 1339 1470
Size: 227 962 150 131
Fragments arranged by size:
962 227 150 131
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1183 1470
Size: 1183 287
EcoO109I rG'GnC_Cy
Cuts at: 0 400 1470
Size: 400 1070
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1369 1470
Size: 1369 101
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 327 359 580 612 1289 1321 1470
Size: 327 32 221 32 677 32 149
Fragments arranged by size:
677 327 221 149 32 32 32
HindIII A'AGCT_T
Cuts at: 0 1097 1470
Size: 1097 373
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 720 996 1470
Size: 720 276 474
Fragments arranged by size:
720 474 276
MscI TGG'CCA
Cuts at: 0 297 1470
Size: 297 1173
MslI CAynn'nnrTG
Cuts at: 0 620 1003 1470
Size: 620 383 467
Fragments arranged by size:
620 467 383
NcoI C'CATG_G
Cuts at: 0 718 1470
Size: 718 752
NheI G'CTAG_C
Cuts at: 0 768 1470
Size: 768 702
PflMI CCAn_nnn'nTGG
Cuts at: 0 754 1470
Size: 754 716
PfoI T'CCnGG_A
Cuts at: 0 598 1470
Size: 598 872
PpuMI rG'GwC_Cy
Cuts at: 0 400 1470
Size: 400 1070
PstI C_TGCA'G
Cuts at: 0 315 940 1425 1470
Size: 315 625 485 45
Fragments arranged by size:
625 485 315 45
SacI G_AGCT'C
Cuts at: 0 327 1470
Size: 327 1143
SapI GCTCTTCn'nnn_
Cuts at: 0 1149 1470
Size: 1149 321
SfcI C'TryA_G
Cuts at: 0 311 404 465 936 1421 1470
Size: 311 93 61 471 485 49
Fragments arranged by size:
485 471 311 93 61 49
SmaI CCC'GGG
Cuts at: 0 397 1470
Size: 397 1073
SmlI C'TyrA_G
Cuts at: 0 1314 1470
Size: 1314 156
StyI C'CwwG_G
Cuts at: 0 718 1470
Size: 718 752
TatI w'GTAC_w
Cuts at: 0 729 1470
Size: 729 741
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1053 1470
Size: 1053 417
XmaI C'CCGG_G
Cuts at: 0 395 1470
Size: 395 1075
Enzymes that do cut:
AclI AcuI AflII AlfI AlwNI ApaLI ApoI AvaI
BanII BbsI BcgI BciVI BglII BlpI Bme1580I BmtI
BpmI Bpu10I BsaBI BsaHI BseRI BsiHKAI BsmI BsmBI
Bsp1286I BsrDI BstYI BtgI DraI EaeI EarI EcoICRI
Eco57MI EcoNI EcoO109I FalI Hin4I HindIII MmeI MscI
MslI NcoI NheI PflMI PfoI PpuMI PstI SacI
SapI SfcI SmaI SmlI StyI TatI XcmI XmaI
Enzymes that do not cut:
AarI AatII AccI Acc65I AfeI AflIII AgeI AhdI
AleI AloI ApaI AscI AseI AsiSI AvrII BaeI
BamHI BanI BbeI BbvCI BclI BfrBI BglI BmgBI
BmrI BplI BpuEI BsaI BsaAI BsaWI BsaXI BseYI
BsgI BsiEI BsiWI BspEI BspHI BspMI BsrBI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstZ17I
Bsu36I BtgZI BtsI ClaI CspCI DraIII DrdI EagI
EciI EcoRI EcoRV FseI FspI FspAI HaeII HincII
HpaI KasI KpnI MfeI MluI MspA1I NaeI NarI
NdeI NgoMIV NotI NruI NsiI NspI PacI PasI
PciI PmeI PmlI PpiI PshAI PsiI PspOMI PspXI
PsrI PvuI PvuII RsrII SacII SalI SanDI SbfI
ScaI SexAI SfiI SfoI SgfI SgrAI SnaBI SpeI
SphI SrfI SspI StuI SwaI TaqII TstI Tth111I
XbaI XhoI XmnI ZraI