(Linear) (Six-base) MAPSORT of: KIEE9688.seq  Check: 8701  from: 1  to: 912



KIEE9688.orf

 With 182 enzymes: SgfI * 

                        December 5, 2014 13:30  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     335     912 
   Size:        335     577
 
ApoI r'AATT_y

Cuts at:      0     688     797     912 
   Size:        688     109     115

  Fragments arranged by size: 

                 688     115     109
 
BglII A'GATC_T

Cuts at:      0      83     564     912 
   Size:         83     481     348

  Fragments arranged by size: 

                 481     348      83
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     357     534     912 
   Size:        357     177     378

  Fragments arranged by size: 

                 378     357     177
 
Bpu10I CC'TnA_GC

Cuts at:      0     878     912 
   Size:        878      34
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     338     912 
   Size:        338     574
 
BsiHKAI G_wGCw'C

Cuts at:      0     599     912 
   Size:        599     313
 
Bsp1286I G_dGCh'C

Cuts at:      0     599     912 
   Size:        599     313
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     335     912 
   Size:        335     577
 
BstYI r'GATC_y

Cuts at:      0      83     564     912 
   Size:         83     481     348

  Fragments arranged by size: 

                 481     348      83
 
DraI TTT'AAA

Cuts at:      0     803     912 
   Size:        803     109
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     357     534     912 
   Size:        357     177     378

  Fragments arranged by size: 

                 378     357     177
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0      39      71     549     581     912 
   Size:         39      32     478      32     331

  Fragments arranged by size: 

                 478     331      39      32      32
 
HincII GTy'rAC

Cuts at:      0     369     651     912 
   Size:        369     282     261

  Fragments arranged by size: 

                 369     282     261
 
HpaI GTT'AAC

Cuts at:      0     369     912 
   Size:        369     543
 
MfeI C'AATT_G

Cuts at:      0     603     912 
   Size:        603     309
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     144     912 
   Size:        144     768
 
PfoI T'CCnGG_A

Cuts at:      0     855     912 
   Size:        855      57
 
PsiI TTA'TAA

Cuts at:      0     778     912 
   Size:        778     134
 
SspI AAT'ATT

Cuts at:      0     792     912 
   Size:        792     120
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     158     190     912 
   Size:        158      32     722

  Fragments arranged by size: 

                 722     158      32
 

 Enzymes that do cut:

     AarI     ApoI    BglII     BpmI   Bpu10I     BsgI  BsiHKAI Bsp1286I
    BspMI    BstYI     DraI  Eco57MI    Hin4I   HincII     HpaI     MfeI
     MmeI     PfoI     PsiI     SspI     TstI

 Enzymes that do not cut: 

    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII   AflIII
     AgeI     AhdI     AleI     AlfI     AloI    AlwNI     ApaI    ApaLI
     AscI     AseI    AsiSI     AvaI    AvrII     BaeI    BamHI     BanI
    BanII     BbeI     BbsI    BbvCI     BcgI    BciVI     BclI    BfrBI
     BglI     BlpI Bme1580I    BmgBI     BmrI     BmtI     BplI    BpuEI
     BsaI    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI    BseRI    BseYI
    BsiEI    BsiWI     BsmI    BsmBI    BspEI    BspHI    BsrBI    BsrDI
    BsrFI    BsrGI   BssHII    BssSI   BstAPI    BstBI   BstEII    BstXI
  BstZ17I   Bsu36I     BtgI    BtgZI     BtsI     ClaI    CspCI   DraIII
     DrdI     EaeI     EagI     EarI     EciI  EcoICRI    EcoNI EcoO109I
    EcoRI    EcoRV     FalI     FseI     FspI    FspAI    HaeII  HindIII
     KasI     KpnI     MluI     MscI     MslI   MspA1I     NaeI     NarI
     NcoI     NdeI   NgoMIV     NheI     NotI     NruI     NsiI     NspI
     PacI     PasI     PciI    PflMI     PmeI     PmlI     PpiI    PpuMI
    PshAI   PspOMI    PspXI     PsrI     PstI     PvuI    PvuII    RsrII
     SacI    SacII     SalI    SanDI     SapI     SbfI     ScaI    SexAI
     SfcI     SfiI     SfoI     SgfI    SgrAI     SmaI     SmlI    SnaBI
     SpeI     SphI     SrfI     StuI     StyI     SwaI    TaqII     TatI
  Tth111I     XbaI     XcmI     XhoI     XmaI     XmnI     ZraI