Restriction map for
pFN21AE9687
(Linear) (Six-base) MAPSORT of: KIEE9687.seq Check: 50 from: 1 to: 687
KIEE9687.orf
With 182 enzymes: SgfI *
December 5, 2014 13:30 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 114 687
Size: 114 573
AlwNI CAG_nnn'CTG
Cuts at: 0 15 687
Size: 15 672
ApaLI G'TGCA_C
Cuts at: 0 271 687
Size: 271 416
AvaI C'yCGr_G
Cuts at: 0 47 301 687
Size: 47 254 386
Fragments arranged by size:
386 254 47
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 110 143 687
Size: 110 33 544
Fragments arranged by size:
544 110 33
BbsI GAAGACnn'nnnn_
Cuts at: 0 306 687
Size: 306 381
BbvCI CC'TCA_GC
Cuts at: 0 170 687
Size: 170 517
BciVI GTATCCnnnnn_n'
Cuts at: 0 111 687
Size: 111 576
BfrBI ATG'CAT
Cuts at: 0 544 687
Size: 544 143
BglI GCCn_nnn'nGGC
Cuts at: 0 18 204 687
Size: 18 186 483
Fragments arranged by size:
483 186 18
BlpI GC'TnA_GC
Cuts at: 0 8 687
Size: 8 679
Bme1580I G_kGCm'C
Cuts at: 0 275 687
Size: 275 412
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 303 687
Size: 303 384
Bpu10I CC'TnA_GC
Cuts at: 0 170 687
Size: 170 517
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 492 687
Size: 492 195
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 187 687
Size: 187 500
BseYI C'CCAG_C
Cuts at: 0 162 687
Size: 162 525
BsiHKAI G_wGCw'C
Cuts at: 0 260 275 687
Size: 260 15 412
Fragments arranged by size:
412 260 15
Bsp1286I G_dGCh'C
Cuts at: 0 260 275 687
Size: 260 15 412
Fragments arranged by size:
412 260 15
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 114 687
Size: 114 573
BsrBI CCG'CTC
Cuts at: 0 147 687
Size: 147 540
BsrFI r'CCGG_y
Cuts at: 0 184 687
Size: 184 503
BtgI C'CryG_G
Cuts at: 0 107 308 687
Size: 107 201 379
Fragments arranged by size:
379 201 107
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 7 687
Size: 7 680
BtsI GCAGTG_nn'
Cuts at: 0 260 687
Size: 260 427
DraIII CAC_nnn'GTG
Cuts at: 0 34 255 687
Size: 34 221 432
Fragments arranged by size:
432 221 34
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 303 687
Size: 303 384
EcoO109I rG'GnC_Cy
Cuts at: 0 137 285 687
Size: 137 148 402
Fragments arranged by size:
402 148 137
FspI TGC'GCA
Cuts at: 0 240 687
Size: 240 447
FspAI rTGC'GCAy
Cuts at: 0 240 687
Size: 240 447
HaeII r_GCGC'y
Cuts at: 0 352 687
Size: 352 335
HindIII A'AGCT_T
Cuts at: 0 89 687
Size: 89 598
MspA1I CmG'CkG
Cuts at: 0 15 195 687
Size: 15 180 492
Fragments arranged by size:
492 180 15
NaeI GCC'GGC
Cuts at: 0 186 687
Size: 186 501
NcoI C'CATG_G
Cuts at: 0 107 687
Size: 107 580
NgoMIV G'CCGG_C
Cuts at: 0 184 687
Size: 184 503
NsiI A_TGCA'T
Cuts at: 0 546 687
Size: 546 141
PflMI CCAn_nnn'nTGG
Cuts at: 0 113 687
Size: 113 574
PpuMI rG'GwC_Cy
Cuts at: 0 137 285 687
Size: 137 148 402
Fragments arranged by size:
402 148 137
PstI C_TGCA'G
Cuts at: 0 212 687
Size: 212 475
PvuII CAG'CTG
Cuts at: 0 195 687
Size: 195 492
ScaI AGT'ACT
Cuts at: 0 390 687
Size: 390 297
SfcI C'TryA_G
Cuts at: 0 208 687
Size: 208 479
SmaI CCC'GGG
Cuts at: 0 49 687
Size: 49 638
SmlI C'TyrA_G
Cuts at: 0 471 687
Size: 471 216
StyI C'CwwG_G
Cuts at: 0 107 687
Size: 107 580
TatI w'GTAC_w
Cuts at: 0 388 687
Size: 388 299
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 501 687
Size: 501 186
XmaI C'CCGG_G
Cuts at: 0 47 687
Size: 47 640
Enzymes that do cut:
AarI AlwNI ApaLI AvaI BaeI BbsI BbvCI BciVI
BfrBI BglI BlpI Bme1580I BpmI Bpu10I BpuEI BseRI
BseYI BsiHKAI Bsp1286I BspMI BsrBI BsrFI BtgI BtgZI
BtsI DraIII Eco57MI EcoO109I FspI FspAI HaeII HindIII
MspA1I NaeI NcoI NgoMIV NsiI PflMI PpuMI PstI
PvuII ScaI SfcI SmaI SmlI StyI TatI XcmI
XmaI
Enzymes that do not cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AloI ApaI ApoI AscI
AseI AsiSI AvrII BamHI BanI BanII BbeI BcgI
BclI BglII BmgBI BmrI BmtI BplI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BsgI BsiEI BsiWI BsmI
BsmBI BspEI BspHI BsrDI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstXI BstYI BstZ17I Bsu36I ClaI CspCI
DraI DrdI EaeI EagI EarI EciI EcoICRI EcoNI
EcoRI EcoRV FalI FseI Hin4I HincII HpaI KasI
KpnI MfeI MluI MmeI MscI MslI NarI NdeI
NheI NotI NruI NspI PacI PasI PciI PfoI
PmeI PmlI PpiI PshAI PsiI PspOMI PspXI PsrI
PvuI RsrII SacI SacII SalI SanDI SapI SbfI
SexAI SfiI SfoI SgfI SgrAI SnaBI SpeI SphI
SrfI SspI StuI SwaI TaqII TstI Tth111I XbaI
XhoI XmnI ZraI