(Linear) (Six-base) MAPSORT of: KIEE9685.seq  Check: 9324  from: 1  to: 933



KIEE9685.orf

 With 182 enzymes: SgfI * 

                        December 5, 2014 13:30  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     752     933 
   Size:        752     181
 
AflIII A'CryG_T

Cuts at:      0     192     933 
   Size:        192     741
 
AlwNI CAG_nnn'CTG

Cuts at:      0     277     933 
   Size:        277     656
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     324     541     637     933 
   Size:        324     217      96     296

  Fragments arranged by size: 

                 324     296     217      96
 
BfrBI ATG'CAT

Cuts at:      0      49     933 
   Size:         49     884
 
BglI GCCn_nnn'nGGC

Cuts at:      0     524     933 
   Size:        524     409
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     720     933 
   Size:        720     213
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     127     659     933 
   Size:        127     532     274

  Fragments arranged by size: 

                 532     274     127
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     910     933 
   Size:        910      23
 
BsaHI Gr'CG_yC

Cuts at:      0     885     933 
   Size:        885      48
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     635     933 
   Size:        635     298
 
BseYI C'CCAG_C

Cuts at:      0      54     933 
   Size:         54     879
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     404     933 
   Size:        404     529
 
BsmI GAATG_Cn'

Cuts at:      0     571     933 
   Size:        571     362
 
BspHI T'CATG_A

Cuts at:      0     206     933 
   Size:        206     727
 
BsrDI GCAATG_nn'

Cuts at:      0      49     933 
   Size:         49     884
 
BssSI C'ACGA_G

Cuts at:      0      38     933 
   Size:         38     895
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0      88     933 
   Size:         88     845
 
DraI TTT'AAA

Cuts at:      0     228     933 
   Size:        228     705
 
EarI CTCTTCn'nnn_

Cuts at:      0     483     597     933 
   Size:        483     114     336

  Fragments arranged by size: 

                 483     336     114
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     127     659     752     933 
   Size:        127     532      93     181

  Fragments arranged by size: 

                 532     181     127      93
 
EcoO109I rG'GnC_Cy

Cuts at:      0     331     933 
   Size:        331     602
 
EcoRV GAT'ATC

Cuts at:      0     204     933 
   Size:        204     729
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     355     387     933 
   Size:        355      32     546

  Fragments arranged by size: 

                 546     355      32
 
HindIII A'AGCT_T

Cuts at:      0     634     933 
   Size:        634     299
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     771     933 
   Size:        771     162
 
NsiI A_TGCA'T

Cuts at:      0      51     933 
   Size:         51     882
 
NspI r_CATG'y

Cuts at:      0     196     933 
   Size:        196     737
 
PasI CC'CwG_GG

Cuts at:      0     857     933 
   Size:        857      76
 
PciI A'CATG_T

Cuts at:      0     192     933 
   Size:        192     741
 
PpuMI rG'GwC_Cy

Cuts at:      0     331     933 
   Size:        331     602
 
ScaI AGT'ACT

Cuts at:      0     506     933 
   Size:        506     427
 
SfcI C'TryA_G

Cuts at:      0     560     711     933 
   Size:        560     151     222

  Fragments arranged by size: 

                 560     222     151
 
SmlI C'TyrA_G

Cuts at:      0     889     933 
   Size:        889      44
 
TatI w'GTAC_w

Cuts at:      0     119     356     391     504     933 
   Size:        119     237      35     113     429

  Fragments arranged by size: 

                 429     237     119     113      35
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     828     933 
   Size:        828     105
 

 Enzymes that do cut:

     AcuI   AflIII    AlwNI     BbsI    BfrBI     BglI     BplI     BpmI
    BpuEI    BsaHI    BseRI    BseYI     BsgI     BsmI    BspHI    BsrDI
    BssSI    BtgZI     DraI     EarI  Eco57MI EcoO109I    EcoRV    Hin4I
  HindIII     MmeI     NsiI     NspI     PasI     PciI    PpuMI     ScaI
     SfcI     SmlI     TatI     XcmI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII     AgeI
     AhdI     AleI     AlfI     AloI     ApaI    ApaLI     ApoI     AscI
     AseI    AsiSI     AvaI    AvrII     BaeI    BamHI     BanI    BanII
     BbeI    BbvCI     BcgI    BciVI     BclI    BglII     BlpI Bme1580I
    BmgBI     BmrI     BmtI   Bpu10I     BsaI    BsaAI    BsaBI    BsaWI
    BsaXI    BsiEI  BsiHKAI    BsiWI    BsmBI Bsp1286I    BspEI    BspMI
    BsrBI    BsrFI    BsrGI   BssHII   BstAPI    BstBI   BstEII    BstXI
    BstYI  BstZ17I   Bsu36I     BtgI     BtsI     ClaI    CspCI   DraIII
     DrdI     EaeI     EagI     EciI  EcoICRI    EcoNI    EcoRI     FalI
     FseI     FspI    FspAI    HaeII   HincII     HpaI     KasI     KpnI
     MfeI     MluI     MscI     MslI   MspA1I     NaeI     NarI     NcoI
     NdeI   NgoMIV     NheI     NotI     NruI     PacI    PflMI     PfoI
     PmeI     PmlI     PpiI    PshAI     PsiI   PspOMI    PspXI     PsrI
     PstI     PvuI    PvuII    RsrII     SacI    SacII     SalI    SanDI
     SapI     SbfI    SexAI     SfiI     SfoI     SgfI    SgrAI     SmaI
    SnaBI     SpeI     SphI     SrfI     SspI     StuI     StyI     SwaI
    TaqII     TstI  Tth111I     XbaI     XhoI     XmaI     XmnI     ZraI