Restriction map for
pFN21AE9683
(Linear) (Six-base) MAPSORT of: KIEE9683.seq Check: 6785 from: 1 to: 1236
KIEE9683.orf
With 182 enzymes: SgfI *
December 5, 2014 13:30 ..
Acc65I G'GTAC_C
Cuts at: 0 855 1236
Size: 855 381
AfeI AGC'GCT
Cuts at: 0 83 1236
Size: 83 1153
AflIII A'CryG_T
Cuts at: 0 312 1236
Size: 312 924
ApaLI G'TGCA_C
Cuts at: 0 617 1236
Size: 617 619
ApoI r'AATT_y
Cuts at: 0 125 255 324 381 884 996 1017 1097
Size: 125 130 69 57 503 112 21 80
Cuts at: 1097 1236
Size: 139
Fragments arranged by size:
503 139 130 125 112 80 69 57
21
AseI AT'TA_AT
Cuts at: 0 173 1236
Size: 173 1063
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 654 687 1236
Size: 654 33 549
Fragments arranged by size:
654 549 33
BanI G'GyrC_C
Cuts at: 0 855 1236
Size: 855 381
BanII G_rGCy'C
Cuts at: 0 557 1236
Size: 557 679
BbsI GAAGACnn'nnnn_
Cuts at: 0 397 1236
Size: 397 839
BciVI GTATCCnnnnn_n'
Cuts at: 0 513 927 1236
Size: 513 414 309
Fragments arranged by size:
513 414 309
Bme1580I G_kGCm'C
Cuts at: 0 621 1236
Size: 621 615
BmrI ACTGGGnnnn_n'
Cuts at: 0 1172 1236
Size: 1172 64
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1067 1099 1236
Size: 1067 32 137
Fragments arranged by size:
1067 137 32
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 508 1236
Size: 508 728
BsaI GGTCTCn'nnnn_
Cuts at: 0 41 1236
Size: 41 1195
BsaAI yAC'GTr
Cuts at: 0 313 460 1236
Size: 313 147 776
Fragments arranged by size:
776 313 147
BsaWI w'CCGG_w
Cuts at: 0 11 1236
Size: 11 1225
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 831 861 1236
Size: 831 30 375
Fragments arranged by size:
831 375 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 760 1236
Size: 760 476
BseYI C'CCAG_C
Cuts at: 0 796 1236
Size: 796 440
BsiHKAI G_wGCw'C
Cuts at: 0 621 1236
Size: 621 615
BsmBI CGTCTCn'nnnn_
Cuts at: 0 55 1236
Size: 55 1181
Bsp1286I G_dGCh'C
Cuts at: 0 557 621 1236
Size: 557 64 615
Fragments arranged by size:
615 557 64
BspEI T'CCGG_A
Cuts at: 0 11 1236
Size: 11 1225
BsrBI CCG'CTC
Cuts at: 0 492 1236
Size: 492 744
BssSI C'ACGA_G
Cuts at: 0 334 1236
Size: 334 902
BstXI CCAn_nnnn'nTGG
Cuts at: 0 51 298 1236
Size: 51 247 938
Fragments arranged by size:
938 247 51
BstYI r'GATC_y
Cuts at: 0 1026 1236
Size: 1026 210
BtsI GCAGTG_nn'
Cuts at: 0 539 1175 1236
Size: 539 636 61
Fragments arranged by size:
636 539 61
DraI TTT'AAA
Cuts at: 0 695 995 1236
Size: 695 300 241
Fragments arranged by size:
695 300 241
EaeI y'GGCC_r
Cuts at: 0 41 1236
Size: 41 1195
EarI CTCTTCn'nnn_
Cuts at: 0 545 1038 1236
Size: 545 493 198
Fragments arranged by size:
545 493 198
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1067 1099 1236
Size: 1067 32 137
Fragments arranged by size:
1067 137 32
HaeII r_GCGC'y
Cuts at: 0 85 1236
Size: 85 1151
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 397 429 748 780 1193 1225 1236
Size: 397 32 319 32 413 32 11
Fragments arranged by size:
413 397 319 32 32 32 11
KpnI G_GTAC'C
Cuts at: 0 859 1236
Size: 859 377
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 797 1236
Size: 797 439
MscI TGG'CCA
Cuts at: 0 43 1236
Size: 43 1193
NspI r_CATG'y
Cuts at: 0 1010 1236
Size: 1010 226
PflMI CCAn_nnn'nTGG
Cuts at: 0 816 1236
Size: 816 420
PmlI CAC'GTG
Cuts at: 0 313 1236
Size: 313 923
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 494 526 1236
Size: 494 32 710
Fragments arranged by size:
710 494 32
PstI C_TGCA'G
Cuts at: 0 77 1236
Size: 77 1159
SapI GCTCTTCn'nnn_
Cuts at: 0 545 1236
Size: 545 691
ScaI AGT'ACT
Cuts at: 0 242 599 1236
Size: 242 357 637
Fragments arranged by size:
637 357 242
SfcI C'TryA_G
Cuts at: 0 73 1236
Size: 73 1163
SmlI C'TyrA_G
Cuts at: 0 487 1236
Size: 487 749
SpeI A'CTAG_T
Cuts at: 0 588 1236
Size: 588 648
SspI AAT'ATT
Cuts at: 0 177 1236
Size: 177 1059
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1191 1236
Size: 1191 45
TatI w'GTAC_w
Cuts at: 0 240 330 597 868 1236
Size: 240 90 267 271 368
Fragments arranged by size:
368 271 267 240 90
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 849 1236
Size: 849 387
XmnI GAAnn'nnTTC
Cuts at: 0 383 1109 1236
Size: 383 726 127
Fragments arranged by size:
726 383 127
Enzymes that do cut:
Acc65I AfeI AflIII ApaLI ApoI AseI BaeI BanI
BanII BbsI BciVI Bme1580I BmrI BpmI BpuEI BsaI
BsaAI BsaWI BsaXI BseRI BseYI BsiHKAI BsmBI Bsp1286I
BspEI BsrBI BssSI BstXI BstYI BtsI DraI EaeI
EarI Eco57MI HaeII Hin4I KpnI MmeI MscI NspI
PflMI PmlI PsrI PstI SapI ScaI SfcI SmlI
SpeI SspI TaqII TatI XcmI XmnI
Enzymes that do not cut:
AarI AatII AccI AclI AcuI AflII AgeI AhdI
AleI AlfI AloI AlwNI ApaI AscI AsiSI AvaI
AvrII BamHI BbeI BbvCI BcgI BclI BfrBI BglI
BglII BlpI BmgBI BmtI BplI Bpu10I BsaBI BsaHI
BsgI BsiEI BsiWI BsmI BspHI BspMI BsrDI BsrFI
BsrGI BssHII BstAPI BstBI BstEII BstZ17I Bsu36I BtgI
BtgZI ClaI CspCI DraIII DrdI EagI EciI EcoICRI
EcoNI EcoO109I EcoRI EcoRV FalI FseI FspI FspAI
HincII HindIII HpaI KasI MfeI MluI MslI MspA1I
NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI
NsiI PacI PasI PciI PfoI PmeI PpiI PpuMI
PshAI PsiI PspOMI PspXI PvuI PvuII RsrII SacI
SacII SalI SanDI SbfI SexAI SfiI SfoI SgfI
SgrAI SmaI SnaBI SphI SrfI StuI StyI SwaI
TstI Tth111I XbaI XhoI XmaI ZraI