(Linear) (Six-base) MAPSORT of: KIEE9681.seq  Check: 603  from: 1  to: 363



KIEE9681.orf

 With 182 enzymes: SgfI * 

                        December 5, 2014 13:30  ..

AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0      16      17      48      49     363 
   Size:         16       1      31       1     314

  Fragments arranged by size: 

                 314      31      16       1       1
 
AlwNI CAG_nnn'CTG

Cuts at:      0     202     363 
   Size:        202     161
 
ApoI r'AATT_y

Cuts at:      0     343     363 
   Size:        343      20
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     313     363 
   Size:        313      50
 
DraI TTT'AAA

Cuts at:      0     192     363 
   Size:        192     171
 
EarI CTCTTCn'nnn_

Cuts at:      0     311     363 
   Size:        311      52
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0      22     363 
   Size:         22     341
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     313     363 
   Size:        313      50
 
EcoRV GAT'ATC

Cuts at:      0     169     363 
   Size:        169     194
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0      84     116     147     179     363 
   Size:         84      32      31      32     184

  Fragments arranged by size: 

                 184      84      32      32      31
 
NspI r_CATG'y

Cuts at:      0     354     363 
   Size:        354       9
 
SfcI C'TryA_G

Cuts at:      0     274     363 
   Size:        274      89
 

 Enzymes that do cut:

     AloI    AlwNI     ApoI     BpmI     DraI     EarI     EciI  Eco57MI
    EcoRV    Hin4I     NspI     SfcI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII
   AflIII     AgeI     AhdI     AleI     AlfI     ApaI    ApaLI     AscI
     AseI    AsiSI     AvaI    AvrII     BaeI    BamHI     BanI    BanII
     BbeI     BbsI    BbvCI     BcgI    BciVI     BclI    BfrBI     BglI
    BglII     BlpI Bme1580I    BmgBI     BmrI     BmtI     BplI   Bpu10I
    BpuEI     BsaI    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI    BseRI
    BseYI     BsgI    BsiEI  BsiHKAI    BsiWI     BsmI    BsmBI Bsp1286I
    BspEI    BspHI    BspMI    BsrBI    BsrDI    BsrFI    BsrGI   BssHII
    BssSI   BstAPI    BstBI   BstEII    BstXI    BstYI  BstZ17I   Bsu36I
     BtgI    BtgZI     BtsI     ClaI    CspCI   DraIII     DrdI     EaeI
     EagI  EcoICRI    EcoNI EcoO109I    EcoRI     FalI     FseI     FspI
    FspAI    HaeII   HincII  HindIII     HpaI     KasI     KpnI     MfeI
     MluI     MmeI     MscI     MslI   MspA1I     NaeI     NarI     NcoI
     NdeI   NgoMIV     NheI     NotI     NruI     NsiI     PacI     PasI
     PciI    PflMI     PfoI     PmeI     PmlI     PpiI    PpuMI    PshAI
     PsiI   PspOMI    PspXI     PsrI     PstI     PvuI    PvuII    RsrII
     SacI    SacII     SalI    SanDI     SapI     SbfI     ScaI    SexAI
     SfiI     SfoI     SgfI    SgrAI     SmaI     SmlI    SnaBI     SpeI
     SphI     SrfI     SspI     StuI     StyI     SwaI    TaqII     TatI
     TstI  Tth111I     XbaI     XcmI     XhoI     XmaI     XmnI     ZraI