Restriction map for
pFN21AE9680
(Linear) (Six-base) MAPSORT of: KIEE9680.seq Check: 5689 from: 1 to: 2421
KIEE9680.orf
With 182 enzymes: SgfI *
December 5, 2014 13:30 ..
AccI GT'mk_AC
Cuts at: 0 1569 2421
Size: 1569 852
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1786 1997 2234 2421
Size: 1786 211 237 187
Fragments arranged by size:
1786 237 211 187
AhdI GACnn_n'nnGTC
Cuts at: 0 1878 2421
Size: 1878 543
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 88 1808 2421
Size: 88 1720 613
Fragments arranged by size:
1720 613 88
AlwNI CAG_nnn'CTG
Cuts at: 0 1681 2290 2421
Size: 1681 609 131
Fragments arranged by size:
1681 609 131
ApaLI G'TGCA_C
Cuts at: 0 1934 2136 2191 2421
Size: 1934 202 55 230
Fragments arranged by size:
1934 230 202 55
ApoI r'AATT_y
Cuts at: 0 718 1129 1263 1599 2421
Size: 718 411 134 336 822
Fragments arranged by size:
822 718 411 336 134
BanII G_rGCy'C
Cuts at: 0 1861 2421
Size: 1861 560
BclI T'GATC_A
Cuts at: 0 1076 2421
Size: 1076 1345
Bme1580I G_kGCm'C
Cuts at: 0 116 1938 2140 2195 2421
Size: 116 1822 202 55 226
Fragments arranged by size:
1822 226 202 116 55
BmrI ACTGGGnnnn_n'
Cuts at: 0 540 1144 1405 2421
Size: 540 604 261 1016
Fragments arranged by size:
1016 604 540 261
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 878 2204 2421
Size: 878 1326 217
Fragments arranged by size:
1326 878 217
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1617 1845 2421
Size: 1617 228 576
Fragments arranged by size:
1617 576 228
BsaHI Gr'CG_yC
Cuts at: 0 35 2421
Size: 35 2386
BsaWI w'CCGG_w
Cuts at: 0 1161 2421
Size: 1161 1260
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 79 109 2421
Size: 79 30 2312
Fragments arranged by size:
2312 79 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 157 1220 2421
Size: 157 1063 1201
Fragments arranged by size:
1201 1063 157
BseYI C'CCAG_C
Cuts at: 0 1796 2127 2323 2421
Size: 1796 331 196 98
Fragments arranged by size:
1796 331 196 98
BsiHKAI G_wGCw'C
Cuts at: 0 1938 2140 2195 2421
Size: 1938 202 55 226
Fragments arranged by size:
1938 226 202 55
BsmI GAATG_Cn'
Cuts at: 0 1824 2421
Size: 1824 597
BsmBI CGTCTCn'nnnn_
Cuts at: 0 99 705 2421
Size: 99 606 1716
Fragments arranged by size:
1716 606 99
Bsp1286I G_dGCh'C
Cuts at: 0 116 1861 1938 2140 2195 2421
Size: 116 1745 77 202 55 226
Fragments arranged by size:
1745 226 202 116 77 55
BsrDI GCAATG_nn'
Cuts at: 0 1066 1302 2421
Size: 1066 236 1119
Fragments arranged by size:
1119 1066 236
BsrGI T'GTAC_A
Cuts at: 0 266 2079 2421
Size: 266 1813 342
Fragments arranged by size:
1813 342 266
BstXI CCAn_nnnn'nTGG
Cuts at: 0 132 2046 2421
Size: 132 1914 375
Fragments arranged by size:
1914 375 132
Bsu36I CC'TnA_GG
Cuts at: 0 230 2421
Size: 230 2191
BtgI C'CryG_G
Cuts at: 0 60 2421
Size: 60 2361
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 2243 2421
Size: 2243 178
BtsI GCAGTG_nn'
Cuts at: 0 1931 1959 2195 2334 2421
Size: 1931 28 236 139 87
Fragments arranged by size:
1931 236 139 87 28
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 1124 1159 2421
Size: 1124 35 1262
Fragments arranged by size:
1262 1124 35
EaeI y'GGCC_r
Cuts at: 0 1980 2100 2421
Size: 1980 120 321
Fragments arranged by size:
1980 321 120
EarI CTCTTCn'nnn_
Cuts at: 0 753 908 968 1464 1733 2421
Size: 753 155 60 496 269 688
Fragments arranged by size:
753 688 496 269 155 60
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 878 1786 1997 2204 2234 2421
Size: 878 908 211 207 30 187
Fragments arranged by size:
908 878 211 207 187 30
HaeII r_GCGC'y
Cuts at: 0 31 1907 2421
Size: 31 1876 514
Fragments arranged by size:
1876 514 31
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 79 111 133 165 670 702 1029 1061
Size: 79 32 22 32 505 32 327 32
Cuts at: 1061 1378 1410 1864 1896 2421
Size: 317 32 454 32 525
Fragments arranged by size:
525 505 454 327 317 79 32 32
32 32 32 32 22
HincII GTy'rAC
Cuts at: 0 249 1570 2421
Size: 249 1321 851
Fragments arranged by size:
1321 851 249
MscI TGG'CCA
Cuts at: 0 1982 2102 2421
Size: 1982 120 319
Fragments arranged by size:
1982 319 120
MslI CAynn'nnrTG
Cuts at: 0 130 1256 2044 2421
Size: 130 1126 788 377
Fragments arranged by size:
1126 788 377 130
MspA1I CmG'CkG
Cuts at: 0 48 62 2077 2421
Size: 48 14 2015 344
Fragments arranged by size:
2015 344 48 14
NspI r_CATG'y
Cuts at: 0 75 2421
Size: 75 2346
PasI CC'CwG_GG
Cuts at: 0 2065 2421
Size: 2065 356
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 146 178 2421
Size: 146 32 2243
Fragments arranged by size:
2243 146 32
PstI C_TGCA'G
Cuts at: 0 556 1677 1926 2421
Size: 556 1121 249 495
Fragments arranged by size:
1121 556 495 249
PvuII CAG'CTG
Cuts at: 0 2077 2421
Size: 2077 344
SacII CC_GC'GG
Cuts at: 0 63 2421
Size: 63 2358
SalI G'TCGA_C
Cuts at: 0 1568 2421
Size: 1568 853
SapI GCTCTTCn'nnn_
Cuts at: 0 1464 1733 2421
Size: 1464 269 688
Fragments arranged by size:
1464 688 269
SfcI C'TryA_G
Cuts at: 0 552 1469 1573 1673 1682 1922 2421
Size: 552 917 104 100 9 240 499
Fragments arranged by size:
917 552 499 240 104 100 9
SmlI C'TyrA_G
Cuts at: 0 1632 1860 2421
Size: 1632 228 561
Fragments arranged by size:
1632 561 228
SspI AAT'ATT
Cuts at: 0 1547 2421
Size: 1547 874
StyI C'CwwG_G
Cuts at: 0 1588 1964 2421
Size: 1588 376 457
Fragments arranged by size:
1588 457 376
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 522 1403 2421
Size: 522 881 1018
Fragments arranged by size:
1018 881 522
TatI w'GTAC_w
Cuts at: 0 266 2079 2421
Size: 266 1813 342
Fragments arranged by size:
1813 342 266
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1307 1339 2130 2162 2421
Size: 1307 32 791 32 259
Fragments arranged by size:
1307 791 259 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 91 1411 2421
Size: 91 1320 1010
Fragments arranged by size:
1320 1010 91
XbaI T'CTAG_A
Cuts at: 0 321 2421
Size: 321 2100
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 738 2421
Size: 738 1683
Enzymes that do cut:
AccI AcuI AhdI AlfI AlwNI ApaLI ApoI BanII
BclI Bme1580I BmrI BpmI BpuEI BsaHI BsaWI BsaXI
BseRI BseYI BsiHKAI BsmI BsmBI Bsp1286I BsrDI BsrGI
BstXI Bsu36I BtgI BtgZI BtsI CspCI EaeI EarI
Eco57MI HaeII Hin4I HincII MscI MslI MspA1I NspI
PasI PsrI PstI PvuII SacII SalI SapI SfcI
SmlI SspI StyI TaqII TatI TstI Tth111I XbaI
XcmI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AflII AflIII AgeI
AleI AloI ApaI AscI AseI AsiSI AvaI AvrII
BaeI BamHI BanI BbeI BbsI BbvCI BcgI BciVI
BfrBI BglI BglII BlpI BmgBI BmtI BplI Bpu10I
BsaI BsaAI BsaBI BsgI BsiEI BsiWI BspEI BspHI
BspMI BsrBI BsrFI BssHII BssSI BstAPI BstBI BstEII
BstYI BstZ17I ClaI DraI DraIII DrdI EagI EciI
EcoICRI EcoNI EcoO109I EcoRI EcoRV FalI FseI FspI
FspAI HindIII HpaI KasI KpnI MfeI MluI MmeI
NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI
NsiI PacI PciI PflMI PfoI PmeI PmlI PpiI
PpuMI PshAI PsiI PspOMI PspXI PvuI RsrII SacI
SanDI SbfI ScaI SexAI SfiI SfoI SgfI SgrAI
SmaI SnaBI SpeI SphI SrfI StuI SwaI XhoI
XmaI XmnI ZraI