Restriction map for
pFN21AE9672
(Linear) (Six-base) MAPSORT of: KIEE9672.seq Check: 9527 from: 1 to: 4494
KIEE9672.orf
With 182 enzymes: SgfI *
March 22, 2012 18:37 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 2157 2454 2497 3936 4494
Size: 2157 297 43 1439 558
Fragments arranged by size:
2157 1439 558 297 43
Acc65I G'GTAC_C
Cuts at: 0 1501 4494
Size: 1501 2993
AclI AA'CG_TT
Cuts at: 0 1667 4494
Size: 1667 2827
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 2980 3154 3643 4494
Size: 2980 174 489 851
Fragments arranged by size:
2980 851 489 174
AflIII A'CryG_T
Cuts at: 0 3431 4494
Size: 3431 1063
AleI CACnn'nnGTG
Cuts at: 0 1079 4494
Size: 1079 3415
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1097 4494
Size: 1097 3397
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 235 267 3773 3805 4494
Size: 235 32 3506 32 689
Fragments arranged by size:
3506 689 235 32 32
AlwNI CAG_nnn'CTG
Cuts at: 0 103 1780 1938 2040 3898 4494
Size: 103 1677 158 102 1858 596
Fragments arranged by size:
1858 1677 596 158 103 102
ApoI r'AATT_y
Cuts at: 0 632 663 1515 2089 3025 3301 3585 4065
Size: 632 31 852 574 936 276 284 480
Cuts at: 4065 4140 4494
Size: 75 354
Fragments arranged by size:
936 852 632 574 480 354 284 276
75 31
AseI AT'TA_AT
Cuts at: 0 922 4494
Size: 922 3572
AvaI C'yCGr_G
Cuts at: 0 517 617 2318 2845 4246 4494
Size: 517 100 1701 527 1401 248
Fragments arranged by size:
1701 1401 527 517 248 100
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 1485 1518 4494
Size: 1485 33 2976
Fragments arranged by size:
2976 1485 33
BamHI G'GATC_C
Cuts at: 0 114 943 4494
Size: 114 829 3551
Fragments arranged by size:
3551 829 114
BanI G'GyrC_C
Cuts at: 0 1368 1501 2145 3852 4494
Size: 1368 133 644 1707 642
Fragments arranged by size:
1707 1368 644 642 133
BanII G_rGCy'C
Cuts at: 0 505 798 1457 2846 3046 3713 4494
Size: 505 293 659 1389 200 667 781
Fragments arranged by size:
1389 781 667 659 505 293 200
BbeI G_GCGC'C
Cuts at: 0 1372 4494
Size: 1372 3122
BbsI GAAGACnn'nnnn_
Cuts at: 0 1666 1742 2555 3631 4169 4494
Size: 1666 76 813 1076 538 325
Fragments arranged by size:
1666 1076 813 538 325 76
BbvCI CC'TCA_GC
Cuts at: 0 3445 3845 4400 4494
Size: 3445 400 555 94
Fragments arranged by size:
3445 555 400 94
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 3057 3091 4090 4124 4494
Size: 3057 34 999 34 370
Fragments arranged by size:
3057 999 370 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 1456 4494
Size: 1456 3038
BclI T'GATC_A
Cuts at: 0 2381 4494
Size: 2381 2113
BfrBI ATG'CAT
Cuts at: 0 428 4494
Size: 428 4066
BglI GCCn_nnn'nGGC
Cuts at: 0 3832 4494
Size: 3832 662
BglII A'GATC_T
Cuts at: 0 3478 4494
Size: 3478 1016
BlpI GC'TnA_GC
Cuts at: 0 1826 4494
Size: 1826 2668
Bme1580I G_kGCm'C
Cuts at: 0 957 4494
Size: 957 3537
BmgBI CAC'GTC
Cuts at: 0 2862 4494
Size: 2862 1632
BmrI ACTGGGnnnn_n'
Cuts at: 0 403 1045 1119 3222 4494
Size: 403 642 74 2103 1272
Fragments arranged by size:
2103 1272 642 403 74
BmtI G_CTAG'C
Cuts at: 0 3808 4494
Size: 3808 686
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 3441 3727 4494
Size: 3441 286 767
Fragments arranged by size:
3441 767 286
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 218 1435 1471 1631 1783 1939 3655 3857
Size: 218 1217 36 160 152 156 1716 202
Cuts at: 3857 4427 4494
Size: 570 67
Fragments arranged by size:
1716 1217 570 218 202 160 156 152
67 36
Bpu10I CC'TnA_GC
Cuts at: 0 3445 3845 4045 4116 4400 4494
Size: 3445 400 200 71 284 94
Fragments arranged by size:
3445 400 284 200 94 71
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 2503 2669 3697 3775 4494
Size: 2503 166 1028 78 719
Fragments arranged by size:
2503 1028 719 166 78
BsaI GGTCTCn'nnnn_
Cuts at: 0 2375 4424 4494
Size: 2375 2049 70
Fragments arranged by size:
2375 2049 70
BsaAI yAC'GTr
Cuts at: 0 4221 4494
Size: 4221 273
BsaHI Gr'CG_yC
Cuts at: 0 1369 2399 3410 4494
Size: 1369 1030 1011 1084
Fragments arranged by size:
1369 1084 1030 1011
BsaWI w'CCGG_w
Cuts at: 0 1784 2981 4494
Size: 1784 1197 1513
Fragments arranged by size:
1784 1513 1197
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 866 896 1183 1213 1276 1306 2488 2518
Size: 866 30 287 30 63 30 1182 30
Cuts at: 2518 4183 4213 4268 4298 4494
Size: 1665 30 55 30 196
Fragments arranged by size:
1665 1182 866 287 196 63 55 30
30 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 2008 2172 2458 4494
Size: 2008 164 286 2036
Fragments arranged by size:
2036 2008 286 164
BseYI C'CCAG_C
Cuts at: 0 118 349 2591 2703 2826 3651 4494
Size: 118 231 2242 112 123 825 843
Fragments arranged by size:
2242 843 825 231 123 118 112
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1354 1664 1861 1924 3930 4185 4494
Size: 1354 310 197 63 2006 255 309
Fragments arranged by size:
2006 1354 310 309 255 197 63
BsiHKAI G_wGCw'C
Cuts at: 0 505 798 856 1457 1900 2317 2498 2763
Size: 505 293 58 601 443 417 181 265
Cuts at: 2763 2846 3317 3713 4051 4220 4494
Size: 83 471 396 338 169 274
Fragments arranged by size:
601 505 471 443 417 396 338 293
274 265 181 169 83 58
BsmI GAATG_Cn'
Cuts at: 0 157 428 2906 4494
Size: 157 271 2478 1588
Fragments arranged by size:
2478 1588 271 157
BsmBI CGTCTCn'nnnn_
Cuts at: 0 2714 3276 4494
Size: 2714 562 1218
Fragments arranged by size:
2714 1218 562
Bsp1286I G_dGCh'C
Cuts at: 0 505 798 856 957 1457 1900 2317 2498
Size: 505 293 58 101 500 443 417 181
Cuts at: 2498 2763 2846 3046 3317 3713 4051 4220 4494
Size: 265 83 200 271 396 338 169 274
Fragments arranged by size:
505 500 443 417 396 338 293 274
271 265 200 181 169 101 83 58
BspEI T'CCGG_A
Cuts at: 0 1784 2981 4494
Size: 1784 1197 1513
Fragments arranged by size:
1784 1513 1197
BspHI T'CATG_A
Cuts at: 0 768 4494
Size: 768 3726
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1677 2157 2454 2497 3936 4494
Size: 1677 480 297 43 1439 558
Fragments arranged by size:
1677 1439 558 480 297 43
BsrDI GCAATG_nn'
Cuts at: 0 1178 1637 3092 3479 4006 4052 4494
Size: 1178 459 1455 387 527 46 442
Fragments arranged by size:
1455 1178 527 459 442 387 46
BsrGI T'GTAC_A
Cuts at: 0 318 714 4494
Size: 318 396 3780
Fragments arranged by size:
3780 396 318
BssSI C'ACGA_G
Cuts at: 0 2800 4494
Size: 2800 1694
BstAPI GCAn_nnn'nTGC
Cuts at: 0 2454 3814 3868 4017 4494
Size: 2454 1360 54 149 477
Fragments arranged by size:
2454 1360 477 149 54
BstBI TT'CG_AA
Cuts at: 0 636 4494
Size: 636 3858
BstXI CCAn_nnnn'nTGG
Cuts at: 0 2925 4494
Size: 2925 1569
BstYI r'GATC_y
Cuts at: 0 51 114 208 943 1214 1606 1844 2805
Size: 51 63 94 735 271 392 238 961
Cuts at: 2805 3478 3647 4494
Size: 673 169 847
Fragments arranged by size:
961 847 735 673 392 271 238 169
94 63 51
Bsu36I CC'TnA_GG
Cuts at: 0 1566 2262 2577 2778 4494
Size: 1566 696 315 201 1716
Fragments arranged by size:
1716 1566 696 315 201
BtgI C'CryG_G
Cuts at: 0 4082 4303 4494
Size: 4082 221 191
Fragments arranged by size:
4082 221 191
BtsI GCAGTG_nn'
Cuts at: 0 390 1596 1681 1781 2700 4372 4411 4494
Size: 390 1206 85 100 919 1672 39 83
Fragments arranged by size:
1672 1206 919 390 100 85 83 39
ClaI AT'CG_AT
Cuts at: 0 3077 4494
Size: 3077 1417
DraI TTT'AAA
Cuts at: 0 659 2054 4139 4494
Size: 659 1395 2085 355
Fragments arranged by size:
2085 1395 659 355
DraIII CAC_nnn'GTG
Cuts at: 0 80 411 712 4494
Size: 80 331 301 3782
Fragments arranged by size:
3782 331 301 80
DrdI GACnn_nn'nnGTC
Cuts at: 0 1990 2866 4314 4494
Size: 1990 876 1448 180
Fragments arranged by size:
1990 1448 876 180
EaeI y'GGCC_r
Cuts at: 0 3824 3903 3966 4494
Size: 3824 79 63 528
Fragments arranged by size:
3824 528 79 63
EarI CTCTTCn'nnn_
Cuts at: 0 439 2812 3130 3574 4246 4494
Size: 439 2373 318 444 672 248
Fragments arranged by size:
2373 672 444 439 318 248
EcoICRI GAG'CTC
Cuts at: 0 503 796 1455 2844 3711 4494
Size: 503 293 659 1389 867 783
Fragments arranged by size:
1389 867 783 659 503 293
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 218 1435 1471 1631 1783 1939 2980 3154
Size: 218 1217 36 160 152 156 1041 174
Cuts at: 3154 3643 3655 3857 4427 4494
Size: 489 12 202 570 67
Fragments arranged by size:
1217 1041 570 489 218 202 174 160
156 152 67 36 12
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1333 1536 2187 2406 3998 4452 4494
Size: 1333 203 651 219 1592 454 42
Fragments arranged by size:
1592 1333 651 454 219 203 42
EcoO109I rG'GnC_Cy
Cuts at: 0 15 621 1767 1794 1914 1992 2700 3441
Size: 15 606 1146 27 120 78 708 741
Cuts at: 3441 4460 4494
Size: 1019 34
Fragments arranged by size:
1146 1019 741 708 606 120 78 34
27 15
EcoRI G'AATT_C
Cuts at: 0 4065 4494
Size: 4065 429
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 708 2640 2808 3517 3738 4494
Size: 708 1932 168 709 221 756
Fragments arranged by size:
1932 756 709 708 221 168
HaeII r_GCGC'y
Cuts at: 0 1019 1372 2008 4494
Size: 1019 353 636 2486
Fragments arranged by size:
2486 1019 636 353
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 866 898 1452 1484 2258 2290 2467 2499
Size: 866 32 554 32 774 32 177 32
Cuts at: 2499 2723 2755 3326 3358 4086 4118 4183 4215
Size: 224 32 571 32 728 32 65 32
Cuts at: 4215 4268 4300 4494
Size: 53 32 194
Fragments arranged by size:
866 774 728 571 554 224 194 177
65 53 32 32 32 32 32 32
32 32 32
HincII GTy'rAC
Cuts at: 0 72 1421 2397 3732 4494
Size: 72 1349 976 1335 762
Fragments arranged by size:
1349 1335 976 762 72
HindIII A'AGCT_T
Cuts at: 0 3463 4494
Size: 3463 1031
KasI G'GCGC_C
Cuts at: 0 1368 4494
Size: 1368 3126
KpnI G_GTAC'C
Cuts at: 0 1505 4494
Size: 1505 2989
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1418 1584 2531 2993 3183 4090 4494
Size: 1418 166 947 462 190 907 404
Fragments arranged by size:
1418 947 907 462 404 190 166
MscI TGG'CCA
Cuts at: 0 3905 3968 4494
Size: 3905 63 526
Fragments arranged by size:
3905 526 63
MslI CAynn'nnrTG
Cuts at: 0 1079 1630 1715 2890 2920 2923 2950 3818
Size: 1079 551 85 1175 30 3 27 868
Cuts at: 3818 4494
Size: 676
Fragments arranged by size:
1175 1079 868 676 551 85 30 27
3
MspA1I CmG'CkG
Cuts at: 0 48 349 1140 1935 2392 2595 3741 3786
Size: 48 301 791 795 457 203 1146 45
Cuts at: 3786 4494
Size: 708
Fragments arranged by size:
1146 795 791 708 457 301 203 48
45
NarI GG'CG_CC
Cuts at: 0 1369 4494
Size: 1369 3125
NheI G'CTAG_C
Cuts at: 0 3804 4494
Size: 3804 690
NsiI A_TGCA'T
Cuts at: 0 430 4494
Size: 430 4064
NspI r_CATG'y
Cuts at: 0 2767 3817 4494
Size: 2767 1050 677
Fragments arranged by size:
2767 1050 677
PasI CC'CwG_GG
Cuts at: 0 3953 4494
Size: 3953 541
PfoI T'CCnGG_A
Cuts at: 0 1448 1925 3791 4494
Size: 1448 477 1866 703
Fragments arranged by size:
1866 1448 703 477
PmlI CAC'GTG
Cuts at: 0 4221 4494
Size: 4221 273
PpuMI rG'GwC_Cy
Cuts at: 0 621 1767 1992 3441 4460 4494
Size: 621 1146 225 1449 1019 34
Fragments arranged by size:
1449 1146 1019 621 225 34
PshAI GACnn'nnGTC
Cuts at: 0 1760 4494
Size: 1760 2734
PsiI TTA'TAA
Cuts at: 0 2107 4494
Size: 2107 2387
PspXI vC'TCGA_Gb
Cuts at: 0 2845 4494
Size: 2845 1649
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 3205 3237 4494
Size: 3205 32 1257
Fragments arranged by size:
3205 1257 32
PstI C_TGCA'G
Cuts at: 0 35 1073 1688 1754 1776 1946 2430 4453
Size: 35 1038 615 66 22 170 484 2023
Cuts at: 4453 4494
Size: 41
Fragments arranged by size:
2023 1038 615 484 170 66 41 35
22
PvuII CAG'CTG
Cuts at: 0 1140 1935 2392 2595 3741 3786 4494
Size: 1140 795 457 203 1146 45 708
Fragments arranged by size:
1146 1140 795 708 457 203 45
SacI G_AGCT'C
Cuts at: 0 505 798 1457 2846 3713 4494
Size: 505 293 659 1389 867 781
Fragments arranged by size:
1389 867 781 659 505 293
SanDI GG'GwC_CC
Cuts at: 0 1767 4494
Size: 1767 2727
SapI GCTCTTCn'nnn_
Cuts at: 0 439 2812 4246 4494
Size: 439 2373 1434 248
Fragments arranged by size:
2373 1434 439 248
SfcI C'TryA_G
Cuts at: 0 31 987 1069 1684 1750 1772 1942 2199
Size: 31 956 82 615 66 22 170 257
Cuts at: 2199 2426 4449 4494
Size: 227 2023 45
Fragments arranged by size:
2023 956 615 257 227 170 82 66
45 31 22
SfoI GGC'GCC
Cuts at: 0 1370 4494
Size: 1370 3124
SmaI CCC'GGG
Cuts at: 0 519 619 2320 4494
Size: 519 100 1701 2174
Fragments arranged by size:
2174 1701 519 100
SmlI C'TyrA_G
Cuts at: 0 2518 2684 2845 3712 3754 4494
Size: 2518 166 161 867 42 740
Fragments arranged by size:
2518 867 740 166 161 42
SspI AAT'ATT
Cuts at: 0 603 920 3600 4494
Size: 603 317 2680 894
Fragments arranged by size:
2680 894 603 317
StyI C'CwwG_G
Cuts at: 0 18 1085 1295 3524 4494
Size: 18 1067 210 2229 970
Fragments arranged by size:
2229 1067 970 210 18
SwaI ATTT'AAAT
Cuts at: 0 2054 4494
Size: 2054 2440
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 422 561 2112 2202 3101 3966 4494
Size: 422 139 1551 90 899 865 528
Fragments arranged by size:
1551 899 865 528 422 139 90
TatI w'GTAC_w
Cuts at: 0 259 318 714 2478 2564 3212 4494
Size: 259 59 396 1764 86 648 1282
Fragments arranged by size:
1764 1282 648 396 259 86 59
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1726 1758 2488 2520 4494
Size: 1726 32 730 32 1974
Fragments arranged by size:
1974 1726 730 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 4311 4494
Size: 4311 183
XbaI T'CTAG_A
Cuts at: 0 2373 3309 4494
Size: 2373 936 1185
Fragments arranged by size:
2373 1185 936
XhoI C'TCGA_G
Cuts at: 0 2845 4494
Size: 2845 1649
XmaI C'CCGG_G
Cuts at: 0 517 617 2318 4494
Size: 517 100 1701 2176
Fragments arranged by size:
2176 1701 517 100
Enzymes that do cut:
AarI Acc65I AclI AcuI AflIII AleI AlfI AloI
AlwNI ApoI AseI AvaI BaeI BamHI BanI BanII
BbeI BbsI BbvCI BcgI BciVI BclI BfrBI BglI
BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI
Bpu10I BpuEI BsaI BsaAI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI
BspMI BsrDI BsrGI BssSI BstAPI BstBI BstXI BstYI
Bsu36I BtgI BtsI ClaI DraI DraIII DrdI EaeI
EarI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI HaeII
Hin4I HincII HindIII KasI KpnI MmeI MscI MslI
MspA1I NarI NheI NsiI NspI PasI PfoI PmlI
PpuMI PshAI PsiI PspXI PsrI PstI PvuII SacI
SanDI SapI SfcI SfoI SmaI SmlI SspI StyI
SwaI TaqII TatI TstI Tth111I XbaI XhoI XmaI
Enzymes that do not cut:
AatII AccI AfeI AflII AgeI AhdI ApaI ApaLI
AscI AsiSI AvrII BsaBI BsiEI BsiWI BsrBI BsrFI
BssHII BstEII BstZ17I BtgZI CspCI EagI EciI EcoRV
FseI FspI FspAI HpaI MfeI MluI NaeI NcoI
NdeI NgoMIV NotI NruI PacI PciI PflMI PmeI
PpiI PspOMI PvuI RsrII SacII SalI SbfI ScaI
SexAI SfiI SgfI SgrAI SnaBI SpeI SphI SrfI
StuI XcmI XmnI ZraI