Restriction map for
pFN21AE9667
(Linear) (Six-base) MAPSORT of: KIEE9667.seq Check: 5730 from: 1 to: 3678
KIEE9667.orf
With 182 enzymes: SgfI *
September 1, 2019 16:00 ..
AccI GT'mk_AC
Cuts at: 0 2240 3678
Size: 2240 1438
Acc65I G'GTAC_C
Cuts at: 0 3421 3678
Size: 3421 257
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 854 1522 1667 1796 2102 3628 3678
Size: 854 668 145 129 306 1526 50
Fragments arranged by size:
1526 854 668 306 145 129 50
AflII C'TTAA_G
Cuts at: 0 121 3678
Size: 121 3557
AgeI A'CCGG_T
Cuts at: 0 162 2546 3678
Size: 162 2384 1132
Fragments arranged by size:
2384 1132 162
AleI CACnn'nnGTG
Cuts at: 0 3007 3678
Size: 3007 671
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 367 3678
Size: 367 3311
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1919 1951 3678
Size: 1919 32 1727
Fragments arranged by size:
1919 1727 32
AlwNI CAG_nnn'CTG
Cuts at: 0 396 3678
Size: 396 3282
ApoI r'AATT_y
Cuts at: 0 133 948 1606 1891 2227 3678
Size: 133 815 658 285 336 1451
Fragments arranged by size:
1451 815 658 336 285 133
AvaI C'yCGr_G
Cuts at: 0 672 980 3079 3461 3678
Size: 672 308 2099 382 217
Fragments arranged by size:
2099 672 382 308 217
BamHI G'GATC_C
Cuts at: 0 802 3678
Size: 802 2876
BanI G'GyrC_C
Cuts at: 0 2905 3421 3678
Size: 2905 516 257
Fragments arranged by size:
2905 516 257
BanII G_rGCy'C
Cuts at: 0 311 660 1843 1962 2156 2267 3678
Size: 311 349 1183 119 194 111 1411
Fragments arranged by size:
1411 1183 349 311 194 119 111
BbeI G_GCGC'C
Cuts at: 0 2909 3678
Size: 2909 769
BbsI GAAGACnn'nnnn_
Cuts at: 0 695 842 1789 2964 3364 3678
Size: 695 147 947 1175 400 314
Fragments arranged by size:
1175 947 695 400 314 147
BbvCI CC'TCA_GC
Cuts at: 0 25 3678
Size: 25 3653
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 3060 3094 3678
Size: 3060 34 584
Fragments arranged by size:
3060 584 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 25 1405 3678
Size: 25 1380 2273
Fragments arranged by size:
2273 1380 25
BglI GCCn_nnn'nGGC
Cuts at: 0 2032 3678
Size: 2032 1646
BglII A'GATC_T
Cuts at: 0 717 3678
Size: 717 2961
BlpI GC'TnA_GC
Cuts at: 0 2539 3678
Size: 2539 1139
Bme1580I G_kGCm'C
Cuts at: 0 3153 3678
Size: 3153 525
BmrI ACTGGGnnnn_n'
Cuts at: 0 101 1530 1967 2419 3667 3678
Size: 101 1429 437 452 1248 11
Fragments arranged by size:
1429 1248 452 437 101 11
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1455 2710 2860 3678
Size: 1455 1255 150 818
Fragments arranged by size:
1455 1255 818 150
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 225 295 1112 3427 3678
Size: 225 70 817 2315 251
Fragments arranged by size:
2315 817 251 225 70
Bpu10I CC'TnA_GC
Cuts at: 0 25 749 2828 3678
Size: 25 724 2079 850
Fragments arranged by size:
2079 850 724 25
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 124 745 892 1879 2451 3678
Size: 124 621 147 987 572 1227
Fragments arranged by size:
1227 987 621 572 147 124
BsaI GGTCTCn'nnnn_
Cuts at: 0 600 1138 3471 3678
Size: 600 538 2333 207
Fragments arranged by size:
2333 600 538 207
BsaAI yAC'GTr
Cuts at: 0 340 3062 3678
Size: 340 2722 616
Fragments arranged by size:
2722 616 340
BsaBI GATnn'nnATC
Cuts at: 0 1090 3678
Size: 1090 2588
BsaHI Gr'CG_yC
Cuts at: 0 2906 3678
Size: 2906 772
BsaWI w'CCGG_w
Cuts at: 0 162 684 2248 2546 3678
Size: 162 522 1564 298 1132
Fragments arranged by size:
1564 1132 522 298 162
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 185 215 682 712 829 859 1032 1062
Size: 185 30 467 30 117 30 173 30
Cuts at: 1062 2191 2221 2699 2729 3678
Size: 1129 30 478 30 949
Fragments arranged by size:
1129 949 478 467 185 173 117 30
30 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 927 2246 2449 2452 2849 3678
Size: 927 1319 203 3 397 829
Fragments arranged by size:
1319 927 829 397 203 3
BseYI C'CCAG_C
Cuts at: 0 1641 1987 2307 3135 3678
Size: 1641 346 320 828 543
Fragments arranged by size:
1641 828 543 346 320
BsiEI CG_ry'CG
Cuts at: 0 2595 2934 3678
Size: 2595 339 744
Fragments arranged by size:
2595 744 339
BsiHKAI G_wGCw'C
Cuts at: 0 65 1986 2156 3678
Size: 65 1921 170 1522
Fragments arranged by size:
1921 1522 170 65
BsmI GAATG_Cn'
Cuts at: 0 3071 3678
Size: 3071 607
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1903 3571 3678
Size: 1903 1668 107
Fragments arranged by size:
1903 1668 107
Bsp1286I G_dGCh'C
Cuts at: 0 65 311 660 1843 1962 1986 2156 2267
Size: 65 246 349 1183 119 24 170 111
Cuts at: 2267 3153 3678
Size: 886 525
Fragments arranged by size:
1183 886 525 349 246 170 119 111
65 24
BspEI T'CCGG_A
Cuts at: 0 2248 3678
Size: 2248 1430
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 2832 3678
Size: 2832 846
BsrBI CCG'CTC
Cuts at: 0 1126 2207 3678
Size: 1126 1081 1471
Fragments arranged by size:
1471 1126 1081
BsrFI r'CCGG_y
Cuts at: 0 162 2546 3678
Size: 162 2384 1132
Fragments arranged by size:
2384 1132 162
BssSI C'ACGA_G
Cuts at: 0 2155 2260 3678
Size: 2155 105 1418
Fragments arranged by size:
2155 1418 105
BstAPI GCAn_nnn'nTGC
Cuts at: 0 2356 3678
Size: 2356 1322
BstEII G'GTnAC_C
Cuts at: 0 1209 2418 3202 3678
Size: 1209 1209 784 476
Fragments arranged by size:
1209 1209 784 476
BstXI CCAn_nnnn'nTGG
Cuts at: 0 88 2061 2181 3678
Size: 88 1973 120 1497
Fragments arranged by size:
1973 1497 120 88
BstYI r'GATC_y
Cuts at: 0 97 425 717 802 864 3410 3525 3678
Size: 97 328 292 85 62 2546 115 153
Fragments arranged by size:
2546 328 292 153 115 97 85 62
BtgI C'CryG_G
Cuts at: 0 7 1462 2060 2211 3445 3678
Size: 7 1455 598 151 1234 233
Fragments arranged by size:
1455 1234 598 233 151 7
BtsI GCAGTG_nn'
Cuts at: 0 187 1852 1958 2885 3678
Size: 187 1665 106 927 793
Fragments arranged by size:
1665 927 793 187 106
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 1713 1748 1839 1874 3017 3052 3678
Size: 1713 35 91 35 1143 35 626
Fragments arranged by size:
1713 1143 626 91 35 35 35
DraI TTT'AAA
Cuts at: 0 1605 3678
Size: 1605 2073
DraIII CAC_nnn'GTG
Cuts at: 0 340 1411 3678
Size: 340 1071 2267
Fragments arranged by size:
2267 1071 340
DrdI GACnn_nn'nnGTC
Cuts at: 0 641 788 1791 2644 3678
Size: 641 147 1003 853 1034
Fragments arranged by size:
1034 1003 853 641 147
EaeI y'GGCC_r
Cuts at: 0 214 887 1353 1626 1667 2012 2592 2606
Size: 214 673 466 273 41 345 580 14
Cuts at: 2606 3581 3678
Size: 975 97
Fragments arranged by size:
975 673 580 466 345 273 214 97
41 14
EagI C'GGCC_G
Cuts at: 0 2592 3678
Size: 2592 1086
EarI CTCTTCn'nnn_
Cuts at: 0 437 925 1831 3678
Size: 437 488 906 1847
Fragments arranged by size:
1847 906 488 437
EcoICRI GAG'CTC
Cuts at: 0 2154 3678
Size: 2154 1524
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 225 295 854 1112 1522 1667 1796 2102
Size: 225 70 559 258 410 145 129 306
Cuts at: 2102 3427 3628 3678
Size: 1325 201 50
Fragments arranged by size:
1325 559 410 306 258 225 201 145
129 70 50
EcoNI CCTnn'n_nnAGG
Cuts at: 0 92 973 3678
Size: 92 881 2705
Fragments arranged by size:
2705 881 92
EcoO109I rG'GnC_Cy
Cuts at: 0 1915 2034 2471 3678
Size: 1915 119 437 1207
Fragments arranged by size:
1915 1207 437 119
EcoRI G'AATT_C
Cuts at: 0 1891 2227 3678
Size: 1891 336 1451
Fragments arranged by size:
1891 1451 336
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 739 886 3609 3678
Size: 739 147 2723 69
Fragments arranged by size:
2723 739 147 69
FspI TGC'GCA
Cuts at: 0 2319 3678
Size: 2319 1359
HaeII r_GCGC'y
Cuts at: 0 2909 3678
Size: 2909 769
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 682 714 829 861 1032 1064 1759 1791
Size: 682 32 115 32 171 32 695 32
Cuts at: 1791 2484 2516 2699 2731 2741 2773 3678
Size: 693 32 183 32 10 32 905
Fragments arranged by size:
905 695 693 682 183 171 115 32
32 32 32 32 32 32 10
HincII GTy'rAC
Cuts at: 0 1341 2241 3158 3303 3378 3678
Size: 1341 900 917 145 75 300
Fragments arranged by size:
1341 917 900 300 145 75
KasI G'GCGC_C
Cuts at: 0 2905 3678
Size: 2905 773
KpnI G_GTAC'C
Cuts at: 0 3425 3678
Size: 3425 253
MscI TGG'CCA
Cuts at: 0 1355 1669 3678
Size: 1355 314 2009
Fragments arranged by size:
2009 1355 314
MslI CAynn'nnrTG
Cuts at: 0 1313 1432 2617 3007 3678
Size: 1313 119 1185 390 671
Fragments arranged by size:
1313 1185 671 390 119
MspA1I CmG'CkG
Cuts at: 0 9 29 623 770 1132 1500 1517 1645
Size: 9 20 594 147 362 368 17 128
Cuts at: 1645 3678
Size: 2033
Fragments arranged by size:
2033 594 368 362 147 128 20 17
9
NarI GG'CG_CC
Cuts at: 0 2906 3678
Size: 2906 772
NcoI C'CATG_G
Cuts at: 0 2060 2211 3678
Size: 2060 151 1467
Fragments arranged by size:
2060 1467 151
NdeI CA'TA_TG
Cuts at: 0 1054 3678
Size: 1054 2624
PasI CC'CwG_GG
Cuts at: 0 281 3678
Size: 281 3397
PfoI T'CCnGG_A
Cuts at: 0 389 437 2743 3352 3413 3678
Size: 389 48 2306 609 61 265
Fragments arranged by size:
2306 609 389 265 61 48
PmlI CAC'GTG
Cuts at: 0 340 3678
Size: 340 3338
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 2254 2286 3678
Size: 2254 32 1392
Fragments arranged by size:
2254 1392 32
PpuMI rG'GwC_Cy
Cuts at: 0 2471 3678
Size: 2471 1207
PshAI GACnn'nnGTC
Cuts at: 0 3373 3678
Size: 3373 305
PstI C_TGCA'G
Cuts at: 0 1791 2929 3173 3678
Size: 1791 1138 244 505
Fragments arranged by size:
1791 1138 505 244
PvuII CAG'CTG
Cuts at: 0 1132 1500 1645 3678
Size: 1132 368 145 2033
Fragments arranged by size:
2033 1132 368 145
SacI G_AGCT'C
Cuts at: 0 2156 3678
Size: 2156 1522
SacII CC_GC'GG
Cuts at: 0 10 3678
Size: 10 3668
SalI G'TCGA_C
Cuts at: 0 2239 3678
Size: 2239 1439
SapI GCTCTTCn'nnn_
Cuts at: 0 437 1831 3678
Size: 437 1394 1847
Fragments arranged by size:
1847 1394 437
ScaI AGT'ACT
Cuts at: 0 230 3678
Size: 230 3448
SexAI A'CCwGG_T
Cuts at: 0 1407 3678
Size: 1407 2271
SfcI C'TryA_G
Cuts at: 0 553 1029 1446 1787 2769 2925 3105 3169
Size: 553 476 417 341 982 156 180 64
Cuts at: 3169 3678
Size: 509
Fragments arranged by size:
982 553 509 476 417 341 180 156
64
SfoI GGC'GCC
Cuts at: 0 2907 3678
Size: 2907 771
SgrAI Cr'CCGG_yG
Cuts at: 0 162 2546 3678
Size: 162 2384 1132
Fragments arranged by size:
2384 1132 162
SmaI CCC'GGG
Cuts at: 0 3463 3678
Size: 3463 215
SmlI C'TyrA_G
Cuts at: 0 121 139 724 871 1858 2466 3678
Size: 121 18 585 147 987 608 1212
Fragments arranged by size:
1212 987 608 585 147 121 18
SspI AAT'ATT
Cuts at: 0 963 3678
Size: 963 2715
StuI AGG'CCT
Cuts at: 0 3605 3678
Size: 3605 73
StyI C'CwwG_G
Cuts at: 0 217 651 798 857 974 1724 2060 2211
Size: 217 434 147 59 117 750 336 151
Cuts at: 2211 3005 3216 3311 3678
Size: 794 211 95 367
Fragments arranged by size:
794 750 434 367 336 217 211 151
147 117 95 59
SwaI ATTT'AAAT
Cuts at: 0 1605 3678
Size: 1605 2073
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 55 2173 2632 3678
Size: 55 2118 459 1046
Fragments arranged by size:
2118 1046 459 55
TatI w'GTAC_w
Cuts at: 0 228 3678
Size: 228 3450
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 185 217 2661 2693 3678
Size: 185 32 2444 32 985
Fragments arranged by size:
2444 985 185 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 183 2862 3678
Size: 183 2679 816
Fragments arranged by size:
2679 816 183
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 855 1221 1995 2287 3313 3432 3678
Size: 855 366 774 292 1026 119 246
Fragments arranged by size:
1026 855 774 366 292 246 119
XmaI C'CCGG_G
Cuts at: 0 3461 3678
Size: 3461 217
XmnI GAAnn'nnTTC
Cuts at: 0 719 3592 3678
Size: 719 2873 86
Fragments arranged by size:
2873 719 86
Enzymes that do cut:
AccI Acc65I AcuI AflII AgeI AleI AlfI AloI
AlwNI ApoI AvaI BamHI BanI BanII BbeI BbsI
BbvCI BcgI BciVI BglI BglII BlpI Bme1580I BmrI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsiEI BsiHKAI BsmI BsmBI
Bsp1286I BspEI BspMI BsrBI BsrFI BssSI BstAPI BstEII
BstXI BstYI BtgI BtsI CspCI DraI DraIII DrdI
EaeI EagI EarI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII KasI KpnI MscI
MslI MspA1I NarI NcoI NdeI PasI PfoI PmlI
PpiI PpuMI PshAI PstI PvuII SacI SacII SalI
SapI ScaI SexAI SfcI SfoI SgrAI SmaI SmlI
SspI StuI StyI SwaI TaqII TatI TstI Tth111I
XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AclI AfeI AflIII AhdI ApaI ApaLI
AscI AseI AsiSI AvrII BaeI BclI BfrBI BmgBI
BmtI BsgI BsiWI BspHI BsrDI BsrGI BssHII BstBI
BstZ17I Bsu36I BtgZI ClaI EciI EcoRV FseI FspAI
HindIII HpaI MfeI MluI MmeI NaeI NgoMIV NheI
NotI NruI NsiI NspI PacI PciI PflMI PmeI
PsiI PspOMI PspXI PsrI PvuI RsrII SanDI SbfI
SfiI SgfI SnaBI SpeI SphI SrfI XbaI XhoI
ZraI