Restriction map for
pFN21AE9663
(Linear) (Six-base) MAPSORT of: KIEE9663.seq Check: 9267 from: 1 to: 1116
KIEE9663.orf
With 182 enzymes: SgfI *
December 27, 2011 16:42 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 543 1116
Size: 543 573
AatII G_ACGT'C
Cuts at: 0 40 1116
Size: 40 1076
AccI GT'mk_AC
Cuts at: 0 1018 1116
Size: 1018 98
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1012 1116
Size: 1012 104
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1102 1116
Size: 1102 14
AlwNI CAG_nnn'CTG
Cuts at: 0 588 1116
Size: 588 528
ApaI G_GGCC'C
Cuts at: 0 465 761 1116
Size: 465 296 355
Fragments arranged by size:
465 355 296
AvaI C'yCGr_G
Cuts at: 0 331 1116
Size: 331 785
AvrII C'CTAG_G
Cuts at: 0 684 1116
Size: 684 432
BamHI G'GATC_C
Cuts at: 0 355 1116
Size: 355 761
BanI G'GyrC_C
Cuts at: 0 132 1116
Size: 132 984
BanII G_rGCy'C
Cuts at: 0 465 761 1116
Size: 465 296 355
Fragments arranged by size:
465 355 296
BglI GCCn_nnn'nGGC
Cuts at: 0 403 1116
Size: 403 713
BlpI GC'TnA_GC
Cuts at: 0 980 1116
Size: 980 136
Bme1580I G_kGCm'C
Cuts at: 0 135 465 532 761 1116
Size: 135 330 67 229 355
Fragments arranged by size:
355 330 229 135 67
BmtI G_CTAG'C
Cuts at: 0 343 1116
Size: 343 773
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 202 718 794 987 1116
Size: 202 516 76 193 129
Fragments arranged by size:
516 202 193 129 76
BsaHI Gr'CG_yC
Cuts at: 0 37 1109 1116
Size: 37 1072 7
Fragments arranged by size:
1072 37 7
BsaWI w'CCGG_w
Cuts at: 0 743 1116
Size: 743 373
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 767 797 1116
Size: 767 30 319
Fragments arranged by size:
767 319 30
BseYI C'CCAG_C
Cuts at: 0 393 1116
Size: 393 723
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 866 1108 1116
Size: 866 242 8
Fragments arranged by size:
866 242 8
Bsp1286I G_dGCh'C
Cuts at: 0 135 465 532 761 1116
Size: 135 330 67 229 355
Fragments arranged by size:
355 330 229 135 67
BspEI T'CCGG_A
Cuts at: 0 743 1116
Size: 743 373
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 543 1116
Size: 543 573
BsrDI GCAATG_nn'
Cuts at: 0 201 923 1116
Size: 201 722 193
Fragments arranged by size:
722 201 193
BsrFI r'CCGG_y
Cuts at: 0 622 1116
Size: 622 494
BsrGI T'GTAC_A
Cuts at: 0 140 1116
Size: 140 976
BstAPI GCAn_nnn'nTGC
Cuts at: 0 243 1116
Size: 243 873
BstYI r'GATC_y
Cuts at: 0 355 1116
Size: 355 761
BtgI C'CryG_G
Cuts at: 0 9 71 795 1116
Size: 9 62 724 321
Fragments arranged by size:
724 321 62 9
DrdI GACnn_nn'nnGTC
Cuts at: 0 42 1116
Size: 42 1074
EaeI y'GGCC_r
Cuts at: 0 146 254 579 1097 1116
Size: 146 108 325 518 19
Fragments arranged by size:
518 325 146 108 19
EarI CTCTTCn'nnn_
Cuts at: 0 260 577 1035 1116
Size: 260 317 458 81
Fragments arranged by size:
458 317 260 81
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 93 1116
Size: 93 1023
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 202 718 794 987 1012 1116
Size: 202 516 76 193 25 104
Fragments arranged by size:
516 202 193 104 76 25
EcoO109I rG'GnC_Cy
Cuts at: 0 461 758 1116
Size: 461 297 358
Fragments arranged by size:
461 358 297
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 588 1116
Size: 588 528
HaeII r_GCGC'y
Cuts at: 0 512 1116
Size: 512 604
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 767 799 963 995 1116
Size: 767 32 164 32 121
Fragments arranged by size:
767 164 121 32 32
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 350 1116
Size: 350 766
MscI TGG'CCA
Cuts at: 0 256 581 1099 1116
Size: 256 325 518 17
Fragments arranged by size:
518 325 256 17
MslI CAynn'nnrTG
Cuts at: 0 1084 1116
Size: 1084 32
MspA1I CmG'CkG
Cuts at: 0 11 172 279 947 984 1116
Size: 11 161 107 668 37 132
Fragments arranged by size:
668 161 132 107 37 11
NaeI GCC'GGC
Cuts at: 0 624 1116
Size: 624 492
NcoI C'CATG_G
Cuts at: 0 795 1116
Size: 795 321
NgoMIV G'CCGG_C
Cuts at: 0 622 1116
Size: 622 494
NheI G'CTAG_C
Cuts at: 0 339 1116
Size: 339 777
PfoI T'CCnGG_A
Cuts at: 0 155 734 1116
Size: 155 579 382
Fragments arranged by size:
579 382 155
PspOMI G'GGCC_C
Cuts at: 0 461 757 1116
Size: 461 296 359
Fragments arranged by size:
461 359 296
PvuII CAG'CTG
Cuts at: 0 279 984 1116
Size: 279 705 132
Fragments arranged by size:
705 279 132
SacII CC_GC'GG
Cuts at: 0 12 1116
Size: 12 1104
SapI GCTCTTCn'nnn_
Cuts at: 0 260 1116
Size: 260 856
StyI C'CwwG_G
Cuts at: 0 576 684 761 795 1116
Size: 576 108 77 34 321
Fragments arranged by size:
576 321 108 77 34
TatI w'GTAC_w
Cuts at: 0 113 140 1046 1116
Size: 113 27 906 70
Fragments arranged by size:
906 113 70 27
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 863 895 1116
Size: 863 32 221
Fragments arranged by size:
863 221 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 366 1116
Size: 366 750
XmnI GAAnn'nnTTC
Cuts at: 0 863 1116
Size: 863 253
ZraI GAC'GTC
Cuts at: 0 38 1116
Size: 38 1078
Enzymes that do cut:
AarI AatII AccI AcuI AlfI AlwNI ApaI AvaI
AvrII BamHI BanI BanII BglI BlpI Bme1580I BmtI
BpmI BsaHI BsaWI BsaXI BseYI BsgI Bsp1286I BspEI
BspMI BsrDI BsrFI BsrGI BstAPI BstYI BtgI DrdI
EaeI EarI EciI Eco57MI EcoO109I FalI HaeII Hin4I
MmeI MscI MslI MspA1I NaeI NcoI NgoMIV NheI
PfoI PspOMI PvuII SacII SapI StyI TatI TstI
XcmI XmnI ZraI
Enzymes that do not cut:
Acc65I AclI AfeI AflII AflIII AgeI AhdI AleI
AloI ApaLI ApoI AscI AseI AsiSI BaeI BbeI
BbsI BbvCI BcgI BciVI BclI BfrBI BglII BmgBI
BmrI BplI Bpu10I BpuEI BsaI BsaAI BsaBI BseRI
BsiEI BsiHKAI BsiWI BsmI BsmBI BspHI BsrBI BssHII
BssSI BstBI BstEII BstXI BstZ17I Bsu36I BtgZI BtsI
ClaI CspCI DraI DraIII EagI EcoICRI EcoNI EcoRI
EcoRV FseI FspI FspAI HincII HindIII HpaI KasI
KpnI MfeI MluI NarI NdeI NotI NruI NsiI
NspI PacI PasI PciI PflMI PmeI PmlI PpiI
PpuMI PshAI PsiI PspXI PsrI PstI PvuI RsrII
SacI SalI SanDI SbfI ScaI SexAI SfcI SfiI
SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SrfI SspI StuI SwaI TaqII Tth111I XbaI XhoI
XmaI