Restriction map for
pFN21AE9662
(Linear) (Six-base) MAPSORT of: KIEE9662.seq Check: 1685 from: 1 to: 1062
KIEE9662.orf
With 182 enzymes: SgfI *
December 27, 2011 16:42 ..
AccI GT'mk_AC
Cuts at: 0 314 775 1062
Size: 314 461 287
Fragments arranged by size:
461 314 287
Acc65I G'GTAC_C
Cuts at: 0 904 1062
Size: 904 158
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 74 1021 1062
Size: 74 947 41
Fragments arranged by size:
947 74 41
AfeI AGC'GCT
Cuts at: 0 873 1062
Size: 873 189
AflIII A'CryG_T
Cuts at: 0 1028 1062
Size: 1028 34
AleI CACnn'nnGTG
Cuts at: 0 694 1062
Size: 694 368
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 826 1062
Size: 826 236
AlwNI CAG_nnn'CTG
Cuts at: 0 1056 1062
Size: 1056 6
ApaI G_GGCC'C
Cuts at: 0 759 1062
Size: 759 303
AvaI C'yCGr_G
Cuts at: 0 880 1062
Size: 880 182
BamHI G'GATC_C
Cuts at: 0 728 1062
Size: 728 334
BanI G'GyrC_C
Cuts at: 0 203 601 904 1062
Size: 203 398 303 158
Fragments arranged by size:
398 303 203 158
BanII G_rGCy'C
Cuts at: 0 183 272 432 759 1062
Size: 183 89 160 327 303
Fragments arranged by size:
327 303 183 160 89
BbeI G_GCGC'C
Cuts at: 0 207 1062
Size: 207 855
BbvCI CC'TCA_GC
Cuts at: 0 413 1062
Size: 413 649
BfrBI ATG'CAT
Cuts at: 0 993 1062
Size: 993 69
BglI GCCn_nnn'nGGC
Cuts at: 0 436 1062
Size: 436 626
Bme1580I G_kGCm'C
Cuts at: 0 498 759 1062
Size: 498 261 303
Fragments arranged by size:
498 303 261
BmgBI CAC'GTC
Cuts at: 0 222 1062
Size: 222 840
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 536 541 646 1062
Size: 536 5 105 416
Fragments arranged by size:
536 416 105 5
Bpu10I CC'TnA_GC
Cuts at: 0 413 1062
Size: 413 649
BsaAI yAC'GTr
Cuts at: 0 99 1062
Size: 99 963
BsaHI Gr'CG_yC
Cuts at: 0 204 1062
Size: 204 858
BsaWI w'CCGG_w
Cuts at: 0 567 598 1062
Size: 567 31 464
Fragments arranged by size:
567 464 31
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 430 721 1062
Size: 430 291 341
Fragments arranged by size:
430 341 291
BseYI C'CCAG_C
Cuts at: 0 135 671 785 1062
Size: 135 536 114 277
Fragments arranged by size:
536 277 135 114
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 796 1042 1062
Size: 796 246 20
Fragments arranged by size:
796 246 20
BsiEI CG_ry'CG
Cuts at: 0 341 1062
Size: 341 721
BsiHKAI G_wGCw'C
Cuts at: 0 183 272 917 1062
Size: 183 89 645 145
Fragments arranged by size:
645 183 145 89
BsiWI C'GTAC_G
Cuts at: 0 321 1062
Size: 321 741
Bsp1286I G_dGCh'C
Cuts at: 0 183 272 432 498 759 917 1062
Size: 183 89 160 66 261 158 145
Fragments arranged by size:
261 183 160 158 145 89 66
BspEI T'CCGG_A
Cuts at: 0 567 1062
Size: 567 495
BsrBI CCG'CTC
Cuts at: 0 6 303 734 1062
Size: 6 297 431 328
Fragments arranged by size:
431 328 297 6
BsrDI GCAATG_nn'
Cuts at: 0 169 1062
Size: 169 893
BsrFI r'CCGG_y
Cuts at: 0 44 677 1062
Size: 44 633 385
Fragments arranged by size:
633 385 44
BssHII G'CGCG_C
Cuts at: 0 292 1062
Size: 292 770
BssSI C'ACGA_G
Cuts at: 0 7 831 1062
Size: 7 824 231
Fragments arranged by size:
824 231 7
BstXI CCAn_nnnn'nTGG
Cuts at: 0 519 1062
Size: 519 543
BstYI r'GATC_y
Cuts at: 0 728 1062
Size: 728 334
Bsu36I CC'TnA_GG
Cuts at: 0 350 454 1062
Size: 350 104 608
Fragments arranged by size:
608 350 104
BtgI C'CryG_G
Cuts at: 0 194 941 1062
Size: 194 747 121
Fragments arranged by size:
747 194 121
DraIII CAC_nnn'GTG
Cuts at: 0 222 1062
Size: 222 840
DrdI GACnn_nn'nnGTC
Cuts at: 0 115 1062
Size: 115 947
EaeI y'GGCC_r
Cuts at: 0 378 403 845 1022 1062
Size: 378 25 442 177 40
Fragments arranged by size:
442 378 177 40 25
EarI CTCTTCn'nnn_
Cuts at: 0 50 862 1062
Size: 50 812 200
Fragments arranged by size:
812 200 50
EcoICRI GAG'CTC
Cuts at: 0 181 270 1062
Size: 181 89 792
Fragments arranged by size:
792 181 89
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 74 536 541 646 1021 1062
Size: 74 462 5 105 375 41
Fragments arranged by size:
462 375 105 74 41 5
EcoO109I rG'GnC_Cy
Cuts at: 0 132 346 630 756 1062
Size: 132 214 284 126 306
Fragments arranged by size:
306 284 214 132 126
HaeII r_GCGC'y
Cuts at: 0 73 207 875 1062
Size: 73 134 668 187
Fragments arranged by size:
668 187 134 73
KasI G'GCGC_C
Cuts at: 0 203 1062
Size: 203 859
KpnI G_GTAC'C
Cuts at: 0 908 1062
Size: 908 154
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 800 1062
Size: 800 262
MscI TGG'CCA
Cuts at: 0 1024 1062
Size: 1024 38
MslI CAynn'nnrTG
Cuts at: 0 694 988 1062
Size: 694 294 74
Fragments arranged by size:
694 294 74
MspA1I CmG'CkG
Cuts at: 0 35 115 139 1062
Size: 35 80 24 923
Fragments arranged by size:
923 80 35 24
NaeI GCC'GGC
Cuts at: 0 46 1062
Size: 46 1016
NarI GG'CG_CC
Cuts at: 0 204 1062
Size: 204 858
NcoI C'CATG_G
Cuts at: 0 941 1062
Size: 941 121
NgoMIV G'CCGG_C
Cuts at: 0 44 1062
Size: 44 1018
NsiI A_TGCA'T
Cuts at: 0 995 1062
Size: 995 67
NspI r_CATG'y
Cuts at: 0 1032 1062
Size: 1032 30
PciI A'CATG_T
Cuts at: 0 1028 1062
Size: 1028 34
PflMI CCAn_nnn'nTGG
Cuts at: 0 222 1062
Size: 222 840
PfoI T'CCnGG_A
Cuts at: 0 230 623 1062
Size: 230 393 439
Fragments arranged by size:
439 393 230
PpuMI rG'GwC_Cy
Cuts at: 0 132 346 1062
Size: 132 214 716
Fragments arranged by size:
716 214 132
PspOMI G'GGCC_C
Cuts at: 0 755 1062
Size: 755 307
PvuI CG_AT'CG
Cuts at: 0 341 1062
Size: 341 721
PvuII CAG'CTG
Cuts at: 0 115 1062
Size: 115 947
SacI G_AGCT'C
Cuts at: 0 183 272 1062
Size: 183 89 790
Fragments arranged by size:
790 183 89
SapI GCTCTTCn'nnn_
Cuts at: 0 862 1062
Size: 862 200
ScaI AGT'ACT
Cuts at: 0 842 1062
Size: 842 220
SexAI A'CCwGG_T
Cuts at: 0 824 1062
Size: 824 238
SfcI C'TryA_G
Cuts at: 0 479 1062
Size: 479 583
SfoI GGC'GCC
Cuts at: 0 205 1062
Size: 205 857
SmaI CCC'GGG
Cuts at: 0 882 1062
Size: 882 180
StyI C'CwwG_G
Cuts at: 0 512 941 1062
Size: 512 429 121
Fragments arranged by size:
512 429 121
TatI w'GTAC_w
Cuts at: 0 840 1062
Size: 840 222
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 338 370 375 407 1062
Size: 338 32 5 32 655
Fragments arranged by size:
655 338 32 32 5
Tth111I GACn'n_nGTC
Cuts at: 0 223 826 1062
Size: 223 603 236
Fragments arranged by size:
603 236 223
XmaI C'CCGG_G
Cuts at: 0 880 1062
Size: 880 182
Enzymes that do cut:
AccI Acc65I AcuI AfeI AflIII AleI AlfI AlwNI
ApaI AvaI BamHI BanI BanII BbeI BbvCI BfrBI
BglI Bme1580I BmgBI BpmI Bpu10I BsaAI BsaHI BsaWI
BseRI BseYI BsgI BsiEI BsiHKAI BsiWI Bsp1286I BspEI
BsrBI BsrDI BsrFI BssHII BssSI BstXI BstYI Bsu36I
BtgI DraIII DrdI EaeI EarI EcoICRI Eco57MI EcoO109I
HaeII KasI KpnI MmeI MscI MslI MspA1I NaeI
NarI NcoI NgoMIV NsiI NspI PciI PflMI PfoI
PpuMI PspOMI PvuI PvuII SacI SapI ScaI SexAI
SfcI SfoI SmaI StyI TatI TstI Tth111I XmaI
Enzymes that do not cut:
AarI AatII AclI AflII AgeI AhdI AloI ApaLI
ApoI AscI AseI AsiSI AvrII BaeI BbsI BcgI
BciVI BclI BglII BlpI BmrI BmtI BplI BpuEI
BsaI BsaBI BsaXI BsmI BsmBI BspHI BspMI BsrGI
BstAPI BstBI BstEII BstZ17I BtgZI BtsI ClaI CspCI
DraI EagI EciI EcoNI EcoRI EcoRV FalI FseI
FspI FspAI Hin4I HincII HindIII HpaI MfeI MluI
NdeI NheI NotI NruI PacI PasI PmeI PmlI
PpiI PshAI PsiI PspXI PsrI PstI RsrII SacII
SalI SanDI SbfI SfiI SgfI SgrAI SmlI SnaBI
SpeI SphI SrfI SspI StuI SwaI TaqII XbaI
XcmI XhoI XmnI ZraI