(Linear) (Six-base) MAPSORT of: KIEE9661.seq  Check: 7634  from: 1  to: 765



KIEE9661.orf

 With 182 enzymes: SgfI * 

                        July 3, 2012 19:03  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      68     765 
   Size:         68     697
 
AlwNI CAG_nnn'CTG

Cuts at:      0     182     448     765 
   Size:        182     266     317

  Fragments arranged by size: 

                 317     266     182
 
BclI T'GATC_A

Cuts at:      0     579     765 
   Size:        579     186
 
BlpI GC'TnA_GC

Cuts at:      0     683     765 
   Size:        683      82
 
Bpu10I CC'TnA_GC

Cuts at:      0     592     765 
   Size:        592     173
 
BsaI GGTCTCn'nnnn_

Cuts at:      0      65     765 
   Size:         65     700
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0      70     217     765 
   Size:         70     147     548

  Fragments arranged by size: 

                 548     147      70
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0     733     765 
   Size:        733      32
 
BstYI r'GATC_y

Cuts at:      0     135     300     765 
   Size:        135     165     465

  Fragments arranged by size: 

                 465     165     135
 
DraI TTT'AAA

Cuts at:      0      96     600     765 
   Size:         96     504     165

  Fragments arranged by size: 

                 504     165      96
 
EarI CTCTTCn'nnn_

Cuts at:      0      47     765 
   Size:         47     718
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      68     765 
   Size:         68     697
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     439     765 
   Size:        439     326
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     196     228     765 
   Size:        196      32     537

  Fragments arranged by size: 

                 537     196      32
 
HincII GTy'rAC

Cuts at:      0     242     765 
   Size:        242     523
 
HpaI GTT'AAC

Cuts at:      0     242     765 
   Size:        242     523
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     249     765 
   Size:        249     516
 
MspA1I CmG'CkG

Cuts at:      0     484     765 
   Size:        484     281
 
PflMI CCAn_nnn'nTGG

Cuts at:      0      81     765 
   Size:         81     684
 
PstI C_TGCA'G

Cuts at:      0      29     765 
   Size:         29     736
 
PvuII CAG'CTG

Cuts at:      0     484     765 
   Size:        484     281
 
SfcI C'TryA_G

Cuts at:      0      25     765 
   Size:         25     740
 
StuI AGG'CCT

Cuts at:      0     761     765 
   Size:        761       4
 

 Enzymes that do cut:

     AcuI    AlwNI     BclI     BlpI   Bpu10I     BsaI    BseRI    BsmBI
    BstYI     DraI     EarI  Eco57MI     FalI    Hin4I   HincII     HpaI
     MmeI   MspA1I    PflMI     PstI    PvuII     SfcI     StuI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII   AflIII
     AgeI     AhdI     AleI     AlfI     AloI     ApaI    ApaLI     ApoI
     AscI     AseI    AsiSI     AvaI    AvrII     BaeI    BamHI     BanI
    BanII     BbeI     BbsI    BbvCI     BcgI    BciVI    BfrBI     BglI
    BglII Bme1580I    BmgBI     BmrI     BmtI     BplI     BpmI    BpuEI
    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI    BseYI     BsgI    BsiEI
  BsiHKAI    BsiWI     BsmI Bsp1286I    BspEI    BspHI    BspMI    BsrBI
    BsrDI    BsrFI    BsrGI   BssHII    BssSI   BstAPI    BstBI   BstEII
    BstXI  BstZ17I   Bsu36I     BtgI    BtgZI     BtsI     ClaI    CspCI
   DraIII     DrdI     EaeI     EagI     EciI  EcoICRI    EcoNI EcoO109I
    EcoRI    EcoRV     FseI     FspI    FspAI    HaeII  HindIII     KasI
     KpnI     MfeI     MluI     MscI     MslI     NaeI     NarI     NcoI
     NdeI   NgoMIV     NheI     NotI     NruI     NsiI     NspI     PacI
     PasI     PciI     PfoI     PmeI     PmlI     PpiI    PpuMI    PshAI
     PsiI   PspOMI    PspXI     PsrI     PvuI    RsrII     SacI    SacII
     SalI    SanDI     SapI     SbfI     ScaI    SexAI     SfiI     SfoI
     SgfI    SgrAI     SmaI     SmlI    SnaBI     SpeI     SphI     SrfI
     SspI     StyI     SwaI    TaqII     TatI     TstI  Tth111I     XbaI
     XcmI     XhoI     XmaI     XmnI     ZraI