Restriction map for
pFN21AE9658
(Linear) (Six-base) MAPSORT of: KIEE9658.seq Check: 3905 from: 1 to: 633
KIEE9658.orf
With 182 enzymes: SgfI *
January 29, 2014 12:07 ..
AatII G_ACGT'C
Cuts at: 0 580 633
Size: 580 53
AccI GT'mk_AC
Cuts at: 0 107 633
Size: 107 526
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 403 633
Size: 403 230
ApaLI G'TGCA_C
Cuts at: 0 448 633
Size: 448 185
ApoI r'AATT_y
Cuts at: 0 289 633
Size: 289 344
BamHI G'GATC_C
Cuts at: 0 512 633
Size: 512 121
BfrBI ATG'CAT
Cuts at: 0 543 633
Size: 543 90
Bme1580I G_kGCm'C
Cuts at: 0 452 633
Size: 452 181
BmrI ACTGGGnnnn_n'
Cuts at: 0 274 633
Size: 274 359
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 88 244 633
Size: 88 156 389
Fragments arranged by size:
389 156 88
BsaAI yAC'GTr
Cuts at: 0 96 606 633
Size: 96 510 27
Fragments arranged by size:
510 96 27
BsaHI Gr'CG_yC
Cuts at: 0 577 633
Size: 577 56
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 297 327 633
Size: 297 30 306
Fragments arranged by size:
306 297 30
BseYI C'CCAG_C
Cuts at: 0 308 633
Size: 308 325
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 187 633
Size: 187 446
BsiHKAI G_wGCw'C
Cuts at: 0 56 275 452 633
Size: 56 219 177 181
Fragments arranged by size:
219 181 177 56
Bsp1286I G_dGCh'C
Cuts at: 0 56 275 452 633
Size: 56 219 177 181
Fragments arranged by size:
219 181 177 56
BsrFI r'CCGG_y
Cuts at: 0 150 633
Size: 150 483
BssHII G'CGCG_C
Cuts at: 0 615 633
Size: 615 18
BssSI C'ACGA_G
Cuts at: 0 453 633
Size: 453 180
BstYI r'GATC_y
Cuts at: 0 485 512 633
Size: 485 27 121
Fragments arranged by size:
485 121 27
BtgI C'CryG_G
Cuts at: 0 113 633
Size: 113 520
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 413 633
Size: 413 220
BtsI GCAGTG_nn'
Cuts at: 0 204 316 633
Size: 204 112 317
Fragments arranged by size:
317 204 112
DraI TTT'AAA
Cuts at: 0 590 633
Size: 590 43
EaeI y'GGCC_r
Cuts at: 0 33 44 633
Size: 33 11 589
Fragments arranged by size:
589 33 11
EarI CTCTTCn'nnn_
Cuts at: 0 605 633
Size: 605 28
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 535 633
Size: 535 98
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 88 244 403 633
Size: 88 156 159 230
Fragments arranged by size:
230 159 156 88
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 8 40 633
Size: 8 32 593
Fragments arranged by size:
593 32 8
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 560 633
Size: 560 73
MscI TGG'CCA
Cuts at: 0 35 633
Size: 35 598
MslI CAynn'nnrTG
Cuts at: 0 542 633
Size: 542 91
MspA1I CmG'CkG
Cuts at: 0 363 633
Size: 363 270
NsiI A_TGCA'T
Cuts at: 0 545 633
Size: 545 88
NspI r_CATG'y
Cuts at: 0 543 633
Size: 543 90
PflMI CCAn_nnn'nTGG
Cuts at: 0 42 633
Size: 42 591
PfoI T'CCnGG_A
Cuts at: 0 65 191 221 407 633
Size: 65 126 30 186 226
Fragments arranged by size:
226 186 126 65 30
PvuII CAG'CTG
Cuts at: 0 363 633
Size: 363 270
SapI GCTCTTCn'nnn_
Cuts at: 0 605 633
Size: 605 28
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 322 633
Size: 322 311
TatI w'GTAC_w
Cuts at: 0 140 633
Size: 140 493
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 297 329 633
Size: 297 32 304
Fragments arranged by size:
304 297 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 474 633
Size: 474 159
ZraI GAC'GTC
Cuts at: 0 578 633
Size: 578 55
Enzymes that do cut:
AatII AccI AcuI ApaLI ApoI BamHI BfrBI Bme1580I
BmrI BpmI BsaAI BsaHI BsaXI BseYI BsgI BsiHKAI
Bsp1286I BsrFI BssHII BssSI BstYI BtgI BtgZI BtsI
DraI EaeI EarI EciI Eco57MI Hin4I MmeI MscI
MslI MspA1I NsiI NspI PflMI PfoI PvuII SapI
TaqII TatI TstI XcmI ZraI
Enzymes that do not cut:
AarI Acc65I AclI AfeI AflII AflIII AgeI AhdI
AleI AlfI AloI AlwNI ApaI AscI AseI AsiSI
AvaI AvrII BaeI BanI BanII BbeI BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI BmgBI BmtI
BplI Bpu10I BpuEI BsaI BsaBI BsaWI BseRI BsiEI
BsiWI BsmI BsmBI BspEI BspHI BspMI BsrBI BsrDI
BsrGI BstAPI BstBI BstEII BstXI BstZ17I Bsu36I ClaI
CspCI DraIII DrdI EagI EcoICRI EcoNI EcoO109I EcoRI
EcoRV FalI FseI FspI FspAI HaeII HincII HindIII
HpaI KasI KpnI MfeI MluI NaeI NarI NcoI
NdeI NgoMIV NheI NotI NruI PacI PasI PciI
PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PspXI
PsrI PstI PvuI RsrII SacI SacII SalI SanDI
SbfI ScaI SexAI SfcI SfiI SfoI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI
StyI SwaI Tth111I XbaI XhoI XmaI XmnI