Restriction map for
pFN21AE9650
(Linear) (Six-base) MAPSORT of: KIEE9650.seq Check: 7677 from: 1 to: 8145
KIEE9650.orf
With 182 enzymes: SgfI *
March 22, 2012 18:37 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 2380 3625 7642 8145
Size: 2380 1245 4017 503
Fragments arranged by size:
4017 2380 1245 503
AatII G_ACGT'C
Cuts at: 0 4200 8145
Size: 4200 3945
AccI GT'mk_AC
Cuts at: 0 184 688 4533 8145
Size: 184 504 3845 3612
Fragments arranged by size:
3845 3612 504 184
AclI AA'CG_TT
Cuts at: 0 6023 8145
Size: 6023 2122
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 212 1310 1828 1896 1934 2779 3008 3122
Size: 212 1098 518 68 38 845 229 114
Cuts at: 3122 3857 4937 6167 6432 6727 6907 7372 7426
Size: 735 1080 1230 265 295 180 465 54
Cuts at: 7426 8145
Size: 719
Fragments arranged by size:
1230 1098 1080 845 735 719 518 465
295 265 229 212 180 114 68 54
38
AfeI AGC'GCT
Cuts at: 0 4219 8145
Size: 4219 3926
AflIII A'CryG_T
Cuts at: 0 5032 6876 8145
Size: 5032 1844 1269
Fragments arranged by size:
5032 1844 1269
AgeI A'CCGG_T
Cuts at: 0 2528 8145
Size: 2528 5617
AhdI GACnn_n'nnGTC
Cuts at: 0 4201 4467 6814 8145
Size: 4201 266 2347 1331
Fragments arranged by size:
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AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 6608 7087 7650 7921 8145
Size: 6608 479 563 271 224
Fragments arranged by size:
6608 563 479 271 224
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1564 1596 4265 4279 4297 4311 8145
Size: 1564 32 2669 14 18 14 3834
Fragments arranged by size:
3834 2669 1564 32 18 14 14
AlwNI CAG_nnn'CTG
Cuts at: 0 645 2611 2946 7000 7693 8011 8145
Size: 645 1966 335 4054 693 318 134
Fragments arranged by size:
4054 1966 693 645 335 318 134
ApaI G_GGCC'C
Cuts at: 0 7315 8145
Size: 7315 830
ApaLI G'TGCA_C
Cuts at: 0 2571 8145
Size: 2571 5574
ApoI r'AATT_y
Cuts at: 0 39 969 1273 1923 1972 2270 2730 4250
Size: 39 930 304 650 49 298 460 1520
Cuts at: 4250 5860 7131 8145
Size: 1610 1271 1014
Fragments arranged by size:
1610 1520 1271 1014 930 650 460 304
298 49 39
AseI AT'TA_AT
Cuts at: 0 5530 8145
Size: 5530 2615
AvaI C'yCGr_G
Cuts at: 0 470 480 711 2143 3625 5848 6212 6572
Size: 470 10 231 1432 1482 2223 364 360
Cuts at: 6572 8145
Size: 1573
Fragments arranged by size:
2223 1573 1482 1432 470 364 360 231
10
AvrII C'CTAG_G
Cuts at: 0 217 1467 8145
Size: 217 1250 6678
Fragments arranged by size:
6678 1250 217
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 257 290 7673 7706 8145
Size: 257 33 7383 33 439
Fragments arranged by size:
7383 439 257 33 33
BamHI G'GATC_C
Cuts at: 0 1020 8145
Size: 1020 7125
BanI G'GyrC_C
Cuts at: 0 484 2818 3248 4864 4872 8145
Size: 484 2334 430 1616 8 3273
Fragments arranged by size:
3273 2334 1616 484 430 8
BanII G_rGCy'C
Cuts at: 0 347 1013 1910 3194 5170 5369 5687 6059
Size: 347 666 897 1284 1976 199 318 372
Cuts at: 6059 6166 6219 6362 6753 7315 8036 8145
Size: 107 53 143 391 562 721 109
Fragments arranged by size:
1976 1284 897 721 666 562 391 372
347 318 199 143 109 107 53
BbeI G_GCGC'C
Cuts at: 0 3252 8145
Size: 3252 4893
BbsI GAAGACnn'nnnn_
Cuts at: 0 1106 1692 2936 3191 3568 4031 4379 6074
Size: 1106 586 1244 255 377 463 348 1695
Cuts at: 6074 6956 7715 7981 8145
Size: 882 759 266 164
Fragments arranged by size:
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348 266 255 164
BbvCI CC'TCA_GC
Cuts at: 0 2221 4034 4667 7751 8145
Size: 2221 1813 633 3084 394
Fragments arranged by size:
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BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 3269 3303 8047 8081 8145
Size: 3269 34 4744 34 64
Fragments arranged by size:
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BclI T'GATC_A
Cuts at: 0 126 2249 3473 5007 7782 8145
Size: 126 2123 1224 1534 2775 363
Fragments arranged by size:
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BfrBI ATG'CAT
Cuts at: 0 5841 8145
Size: 5841 2304
BglI GCCn_nnn'nGGC
Cuts at: 0 844 7009 8145
Size: 844 6165 1136
Fragments arranged by size:
6165 1136 844
BglII A'GATC_T
Cuts at: 0 1980 4858 5895 8145
Size: 1980 2878 1037 2250
Fragments arranged by size:
2878 2250 1980 1037
BlpI GC'TnA_GC
Cuts at: 0 5137 6230 7289 8145
Size: 5137 1093 1059 856
Fragments arranged by size:
5137 1093 1059 856
Bme1580I G_kGCm'C
Cuts at: 0 487 2575 3408 4666 6608 7315 7586 8070
Size: 487 2088 833 1258 1942 707 271 484
Cuts at: 8070 8100 8118 8145
Size: 30 18 27
Fragments arranged by size:
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30 27 18
BmgBI CAC'GTC
Cuts at: 0 1713 8145
Size: 1713 6432
BmrI ACTGGGnnnn_n'
Cuts at: 0 568 1578 3529 3739 3811 4708 6694 8057
Size: 568 1010 1951 210 72 897 1986 1363
Cuts at: 8057 8145
Size: 88
Fragments arranged by size:
1986 1951 1363 1010 897 568 210 88
72
BmtI G_CTAG'C
Cuts at: 0 3014 3613 8145
Size: 3014 599 4532
Fragments arranged by size:
4532 3014 599
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 4768 6606 7352 8145
Size: 4768 1838 746 793
Fragments arranged by size:
4768 1838 793 746
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 290 842 1794 1852 1951 3493 3946 4039
Size: 290 552 952 58 99 1542 453 93
Cuts at: 4039 4669 5780 6485 6601 7969 8145
Size: 630 1111 705 116 1368 176
Fragments arranged by size:
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290 176 116 99 93 58
Bpu10I CC'TnA_GC
Cuts at: 0 2221 2378 2666 4034 4667 7016 7084 7201
Size: 2221 157 288 1368 633 2349 68 117
Cuts at: 7201 7543 7751 8145
Size: 342 208 394
Fragments arranged by size:
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157 117 68
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1366 4642 5294 5685 6990 8145
Size: 1366 3276 652 391 1305 1155
Fragments arranged by size:
3276 1366 1305 1155 652 391
BsaI GGTCTCn'nnnn_
Cuts at: 0 299 1948 4739 5385 8145
Size: 299 1649 2791 646 2760
Fragments arranged by size:
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BsaAI yAC'GTr
Cuts at: 0 1232 6318 8145
Size: 1232 5086 1827
Fragments arranged by size:
5086 1827 1232
BsaBI GATnn'nnATC
Cuts at: 0 1253 5009 8145
Size: 1253 3756 3136
Fragments arranged by size:
3756 3136 1253
BsaHI Gr'CG_yC
Cuts at: 0 1673 3249 4197 6534 7654 8145
Size: 1673 1576 948 2337 1120 491
Fragments arranged by size:
2337 1673 1576 1120 948 491
BsaWI w'CCGG_w
Cuts at: 0 1583 2528 2867 3698 8145
Size: 1583 945 339 831 4447
Fragments arranged by size:
4447 1583 945 831 339
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1564 1594 6996 7026 7981 8011 8145
Size: 1564 30 5402 30 955 30 134
Fragments arranged by size:
5402 1564 955 134 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 272 275 522 688 865 1192 1918 2008
Size: 272 3 247 166 177 327 726 90
Cuts at: 2008 2278 5315 5611 5623 5635 6166 6867 7170
Size: 270 3037 296 12 12 531 701 303
Cuts at: 7170 7600 8145
Size: 430 545
Fragments arranged by size:
3037 726 701 545 531 430 327 303
296 272 270 247 177 166 90 12
12 3
BseYI C'CCAG_C
Cuts at: 0 2772 3323 3909 4699 4811 4847 7060 7420
Size: 2772 551 586 790 112 36 2213 360
Cuts at: 7420 8017 8035 8104 8145
Size: 597 18 69 41
Fragments arranged by size:
2772 2213 790 597 586 551 360 112
69 41 36 18
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 338 498 800 4713 5499 6029 7620 8145
Size: 338 160 302 3913 786 530 1591 525
Fragments arranged by size:
3913 1591 786 530 525 338 302 160
BsiEI CG_ry'CG
Cuts at: 0 2529 3138 8145
Size: 2529 609 5007
Fragments arranged by size:
5007 2529 609
BsiHKAI G_wGCw'C
Cuts at: 0 425 1013 1210 1910 2575 3194 4658 5138
Size: 425 588 197 700 665 619 1464 480
Cuts at: 5138 5369 6059 6166 6219 6976 8145
Size: 231 690 107 53 757 1169
Fragments arranged by size:
1464 1169 757 700 690 665 619 588
480 425 231 197 107 53
BsmI GAATG_Cn'
Cuts at: 0 1608 8145
Size: 1608 6537
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1664 2080 4195 6098 7661 8145
Size: 1664 416 2115 1903 1563 484
Fragments arranged by size:
2115 1903 1664 1563 484 416
Bsp1286I G_dGCh'C
Cuts at: 0 347 425 487 1013 1210 1910 2575 3194
Size: 347 78 62 526 197 700 665 619
Cuts at: 3194 3408 4658 4666 5138 5170 5369 5687 6059
Size: 214 1250 8 472 32 199 318 372
Cuts at: 6059 6166 6219 6362 6608 6753 6976 7315 7586
Size: 107 53 143 246 145 223 339 271
Cuts at: 7586 8036 8070 8100 8118 8145
Size: 450 34 30 18 27
Fragments arranged by size:
1250 700 665 619 526 472 450 372
347 339 318 271 246 223 214 199
197 145 143 107 78 62 53 34
32 30 27 18 8
BspEI T'CCGG_A
Cuts at: 0 1583 2867 3698 8145
Size: 1583 1284 831 4447
Fragments arranged by size:
4447 1583 1284 831
BspHI T'CATG_A
Cuts at: 0 4799 7403 8145
Size: 4799 2604 742
Fragments arranged by size:
4799 2604 742
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1989 2380 2626 3317 3625 4757 7642 7803
Size: 1989 391 246 691 308 1132 2885 161
Cuts at: 7803 8145
Size: 342
Fragments arranged by size:
2885 1989 1132 691 391 342 308 246
161
BsrBI CCG'CTC
Cuts at: 0 1317 1575 2581 3283 5794 7205 8145
Size: 1317 258 1006 702 2511 1411 940
Fragments arranged by size:
2511 1411 1317 1006 940 702 258
BsrFI r'CCGG_y
Cuts at: 0 2528 3329 4241 8145
Size: 2528 801 912 3904
Fragments arranged by size:
3904 2528 912 801
BsrGI T'GTAC_A
Cuts at: 0 1664 8145
Size: 1664 6481
BssSI C'ACGA_G
Cuts at: 0 3237 6849 8145
Size: 3237 3612 1296
Fragments arranged by size:
3612 3237 1296
BstAPI GCAn_nnn'nTGC
Cuts at: 0 2190 8145
Size: 2190 5955
BstEII G'GTnAC_C
Cuts at: 0 4264 8145
Size: 4264 3881
BstXI CCAn_nnnn'nTGG
Cuts at: 0 3777 3824 4721 5242 5770 7594 8145
Size: 3777 47 897 521 528 1824 551
Fragments arranged by size:
3777 1824 897 551 528 521 47
BstYI r'GATC_y
Cuts at: 0 887 1020 1254 1980 3241 3760 3812 4858
Size: 887 133 234 726 1261 519 52 1046
Cuts at: 4858 5895 6476 8145
Size: 1037 581 1669
Fragments arranged by size:
1669 1261 1046 1037 887 726 581 519
234 133 52
BstZ17I GTA'TAC
Cuts at: 0 185 8145
Size: 185 7960
Bsu36I CC'TnA_GG
Cuts at: 0 671 5249 6363 6986 8145
Size: 671 4578 1114 623 1159
Fragments arranged by size:
4578 1159 1114 671 623
BtgI C'CryG_G
Cuts at: 0 1524 1617 5636 5927 6600 7622 8145
Size: 1524 93 4019 291 673 1022 523
Fragments arranged by size:
4019 1524 1022 673 523 291 93
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1014 8145
Size: 1014 7131
BtsI GCAGTG_nn'
Cuts at: 0 3672 4760 5382 5814 5942 6370 6402 7643
Size: 3672 1088 622 432 128 428 32 1241
Cuts at: 7643 7694 8145
Size: 51 451
Fragments arranged by size:
3672 1241 1088 622 451 432 428 128
51 32
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 557 592 4327 4362 8145
Size: 557 35 3735 35 3783
Fragments arranged by size:
3783 3735 557 35 35
DraI TTT'AAA
Cuts at: 0 44 5967 5987 8145
Size: 44 5923 20 2158
Fragments arranged by size:
5923 2158 44 20
DraIII CAC_nnn'GTG
Cuts at: 0 6321 8145
Size: 6321 1824
DrdI GACnn_nn'nnGTC
Cuts at: 0 2557 4956 7613 8145
Size: 2557 2399 2657 532
Fragments arranged by size:
2657 2557 2399 532
EaeI y'GGCC_r
Cuts at: 0 2394 3354 4162 4508 5406 6271 8145
Size: 2394 960 808 346 898 865 1874
Fragments arranged by size:
2394 1874 960 898 865 808 346
EarI CTCTTCn'nnn_
Cuts at: 0 128 188 499 1001 1289 1915 4283 5600
Size: 128 60 311 502 288 626 2368 1317
Cuts at: 5600 6143 6828 7205 7520 7954 8145
Size: 543 685 377 315 434 191
Fragments arranged by size:
2368 1317 685 626 543 502 434 377
315 311 288 191 128 60
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 862 4164 6873 6885 8145
Size: 862 3302 2709 12 1260
Fragments arranged by size:
3302 2709 1260 862 12
EcoICRI GAG'CTC
Cuts at: 0 1011 1908 3192 5367 6057 6164 6217 8145
Size: 1011 897 1284 2175 690 107 53 1928
Fragments arranged by size:
2175 1928 1284 1011 897 690 107 53
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 212 290 842 1310 1794 1828 1852 1896
Size: 212 78 552 468 484 34 24 44
Cuts at: 1896 1934 1951 2779 3008 3122 3493 3857 3946
Size: 38 17 828 229 114 371 364 89
Cuts at: 3946 4039 4669 4937 5780 6167 6432 6485 6601
Size: 93 630 268 843 387 265 53 116
Cuts at: 6601 6727 6907 7372 7426 7969 8145
Size: 126 180 465 54 543 176
Fragments arranged by size:
843 828 630 552 543 484 468 465
387 371 364 268 265 229 212 180
176 126 116 114 93 89 78 54
53 44 38 34 24 17
EcoNI CCTnn'n_nnAGG
Cuts at: 0 2167 4004 6044 7766 8145
Size: 2167 1837 2040 1722 379
Fragments arranged by size:
2167 2040 1837 1722 379
EcoO109I rG'GnC_Cy
Cuts at: 0 177 642 747 2859 3986 6576 6674 6997
Size: 177 465 105 2112 1127 2590 98 323
Cuts at: 6997 7312 8145
Size: 315 833
Fragments arranged by size:
2590 2112 1127 833 465 323 315 177
105 98
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 3030 3141 4440 6507 8145
Size: 3030 111 1299 2067 1638
Fragments arranged by size:
3030 2067 1638 1299 111
HaeII r_GCGC'y
Cuts at: 0 3252 3273 4221 6710 8145
Size: 3252 21 948 2489 1435
Fragments arranged by size:
3252 2489 1435 948 21
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 82 114 2896 2928 3205 3237 3937 3969
Size: 82 32 2782 32 277 32 700 32
Cuts at: 3969 3973 4005 4187 4219 4453 4485 5562 5594
Size: 4 32 182 32 234 32 1077 32
Cuts at: 5594 6046 6078 6800 6832 7180 7212 7366 7398
Size: 452 32 722 32 348 32 154 32
Cuts at: 7398 8145
Size: 747
Fragments arranged by size:
2782 1077 747 722 700 452 348 277
234 182 154 82 32 32 32 32
32 32 32 32 32 32 32 32
4
HindIII A'AGCT_T
Cuts at: 0 1843 2350 3123 5553 5745 8145
Size: 1843 507 773 2430 192 2400
Fragments arranged by size:
2430 2400 1843 773 507 192
KasI G'GCGC_C
Cuts at: 0 3248 8145
Size: 3248 4897
MfeI C'AATT_G
Cuts at: 0 785 8145
Size: 785 7360
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 4166 4451 8145
Size: 4166 285 3694
Fragments arranged by size:
4166 3694 285
MscI TGG'CCA
Cuts at: 0 2396 4164 4510 5408 6273 8145
Size: 2396 1768 346 898 865 1872
Fragments arranged by size:
2396 1872 1768 898 865 346
MslI CAynn'nnrTG
Cuts at: 0 2098 2212 3391 3644 3862 3878 5591 8145
Size: 2098 114 1179 253 218 16 1713 2554
Fragments arranged by size:
2554 2098 1713 1179 253 218 114 16
MspA1I CmG'CkG
Cuts at: 0 1395 2322 2608 3742 4671 4689 4815 5412
Size: 1395 927 286 1134 929 18 126 597
Cuts at: 5412 6234 7498 8021 8039 8145
Size: 822 1264 523 18 106
Fragments arranged by size:
1395 1264 1134 929 927 822 597 523
286 126 106 18 18
NaeI GCC'GGC
Cuts at: 0 4243 8145
Size: 4243 3902
NarI GG'CG_CC
Cuts at: 0 3249 8145
Size: 3249 4896
NcoI C'CATG_G
Cuts at: 0 5636 5927 7622 8145
Size: 5636 291 1695 523
Fragments arranged by size:
5636 1695 523 291
NdeI CA'TA_TG
Cuts at: 0 1218 8145
Size: 1218 6927
NgoMIV G'CCGG_C
Cuts at: 0 4241 8145
Size: 4241 3904
NheI G'CTAG_C
Cuts at: 0 3010 3609 8145
Size: 3010 599 4536
Fragments arranged by size:
4536 3010 599
NruI TCG'CGA
Cuts at: 0 1358 8145
Size: 1358 6787
NsiI A_TGCA'T
Cuts at: 0 5843 8145
Size: 5843 2302
NspI r_CATG'y
Cuts at: 0 1312 2193 5036 6880 7551 8145
Size: 1312 881 2843 1844 671 594
Fragments arranged by size:
2843 1844 1312 881 671 594
PasI CC'CwG_GG
Cuts at: 0 1677 6955 8145
Size: 1677 5278 1190
Fragments arranged by size:
5278 1677 1190
PciI A'CATG_T
Cuts at: 0 5032 6876 8145
Size: 5032 1844 1269
Fragments arranged by size:
5032 1844 1269
PfoI T'CCnGG_A
Cuts at: 0 541 908 1382 3923 4823 5448 7854 8145
Size: 541 367 474 2541 900 625 2406 291
Fragments arranged by size:
2541 2406 900 625 541 474 367 291
PmlI CAC'GTG
Cuts at: 0 1232 6318 8145
Size: 1232 5086 1827
Fragments arranged by size:
5086 1827 1232
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1564 1596 8145
Size: 1564 32 6549
Fragments arranged by size:
6549 1564 32
PpuMI rG'GwC_Cy
Cuts at: 0 177 747 2859 3986 6674 8145
Size: 177 570 2112 1127 2688 1471
Fragments arranged by size:
2688 2112 1471 1127 570 177
PsiI TTA'TAA
Cuts at: 0 3434 8145
Size: 3434 4711
PspOMI G'GGCC_C
Cuts at: 0 7311 8145
Size: 7311 834
PspXI vC'TCGA_Gb
Cuts at: 0 6212 8145
Size: 6212 1933
PstI C_TGCA'G
Cuts at: 0 121 528 2015 2327 2951 3966 5377 8145
Size: 121 407 1487 312 624 1015 1411 2768
Fragments arranged by size:
2768 1487 1411 1015 624 407 312 121
PvuII CAG'CTG
Cuts at: 0 1395 2322 2608 3742 4671 4689 4815 5412
Size: 1395 927 286 1134 929 18 126 597
Cuts at: 5412 6234 7498 8021 8145
Size: 822 1264 523 124
Fragments arranged by size:
1395 1264 1134 929 927 822 597 523
286 126 124 18
SacI G_AGCT'C
Cuts at: 0 1013 1910 3194 5369 6059 6166 6219 8145
Size: 1013 897 1284 2175 690 107 53 1926
Fragments arranged by size:
2175 1926 1284 1013 897 690 107 53
SanDI GG'GwC_CC
Cuts at: 0 6674 8145
Size: 6674 1471
SapI GCTCTTCn'nnn_
Cuts at: 0 1001 1915 8145
Size: 1001 914 6230
Fragments arranged by size:
6230 1001 914
ScaI AGT'ACT
Cuts at: 0 2128 2405 3686 6142 8145
Size: 2128 277 1281 2456 2003
Fragments arranged by size:
2456 2128 2003 1281 277
SexAI A'CCwGG_T
Cuts at: 0 1193 4260 7430 8145
Size: 1193 3067 3170 715
Fragments arranged by size:
3170 3067 1193 715
SfcI C'TryA_G
Cuts at: 0 117 524 614 689 2011 2323 2947 3962
Size: 117 407 90 75 1322 312 624 1015
Cuts at: 3962 4374 4833 5373 5776 6202 6238 8145
Size: 412 459 540 403 426 36 1907
Fragments arranged by size:
1907 1322 1015 624 540 459 426 412
407 403 312 117 90 75 36
SfoI GGC'GCC
Cuts at: 0 3250 8145
Size: 3250 4895
SmaI CCC'GGG
Cuts at: 0 472 8145
Size: 472 7673
SmlI C'TyrA_G
Cuts at: 0 1345 2143 4657 5309 5700 5848 6212 6969
Size: 1345 798 2514 652 391 148 364 757
Cuts at: 6969 8145
Size: 1176
Fragments arranged by size:
2514 1345 1176 798 757 652 391 364
148
SpeI A'CTAG_T
Cuts at: 0 7807 8145
Size: 7807 338
SphI G_CATG'C
Cuts at: 0 7551 8145
Size: 7551 594
SspI AAT'ATT
Cuts at: 0 1508 8145
Size: 1508 6637
StuI AGG'CCT
Cuts at: 0 478 2588 8145
Size: 478 2110 5557
Fragments arranged by size:
5557 2110 478
StyI C'CwwG_G
Cuts at: 0 217 398 416 488 1157 1467 2168 3005
Size: 217 181 18 72 669 310 701 837
Cuts at: 3005 4269 5636 5715 5879 5927 5995 6753 7232
Size: 1264 1367 79 164 48 68 758 479
Cuts at: 7232 7622 8145
Size: 390 523
Fragments arranged by size:
1367 1264 837 758 701 669 523 479
390 310 217 181 164 79 72 68
48 18
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 935 3152 4882 6613 7635 8018 8145
Size: 935 2217 1730 1731 1022 383 127
Fragments arranged by size:
2217 1731 1730 1022 935 383 127
TatI w'GTAC_w
Cuts at: 0 1664 2126 2403 3416 3684 3707 6140 8145
Size: 1664 462 277 1013 268 23 2433 2005
Fragments arranged by size:
2433 2005 1664 1013 462 277 268 23
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 2339 2371 3905 3937 5407 5439 5934 5966
Size: 2339 32 1534 32 1470 32 495 32
Cuts at: 5966 5991 6023 6996 7028 8145
Size: 25 32 973 32 1117
Fragments arranged by size:
2339 1534 1470 1117 973 495 32 32
32 32 32 32 25
Tth111I GACn'n_nGTC
Cuts at: 0 537 8145
Size: 537 7608
XbaI T'CTAG_A
Cuts at: 0 2274 5188 8145
Size: 2274 2914 2957
Fragments arranged by size:
2957 2914 2274
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 2769 3824 6802 8145
Size: 2769 1055 2978 1343
Fragments arranged by size:
2978 2769 1343 1055
XhoI C'TCGA_G
Cuts at: 0 2143 5848 6212 8145
Size: 2143 3705 364 1933
Fragments arranged by size:
3705 2143 1933 364
XmaI C'CCGG_G
Cuts at: 0 470 8145
Size: 470 7675
XmnI GAAnn'nnTTC
Cuts at: 0 972 1283 1508 8145
Size: 972 311 225 6637
Fragments arranged by size:
6637 972 311 225
ZraI GAC'GTC
Cuts at: 0 4198 8145
Size: 4198 3947
Enzymes that do cut:
AarI AatII AccI AclI AcuI AfeI AflIII AgeI
AhdI AlfI AloI AlwNI ApaI ApaLI ApoI AseI
AvaI AvrII BaeI BamHI BanI BanII BbeI BbsI
BbvCI BcgI BclI BfrBI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrFI BsrGI BssSI BstAPI BstEII BstXI BstYI
BstZ17I Bsu36I BtgI BtgZI BtsI CspCI DraI DraIII
DrdI EaeI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
FalI HaeII Hin4I HindIII KasI MfeI MmeI MscI
MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI
NruI NsiI NspI PasI PciI PfoI PmlI PpiI
PpuMI PsiI PspOMI PspXI PstI PvuII SacI SanDI
SapI ScaI SexAI SfcI SfoI SmaI SmlI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
Acc65I AflII AleI AscI AsiSI BciVI BsiWI BsrDI
BssHII BstBI ClaI EagI EcoRI EcoRV FseI FspI
FspAI HincII HpaI KpnI MluI NotI PacI PflMI
PmeI PshAI PsrI PvuI RsrII SacII SalI SbfI
SfiI SgfI SgrAI SnaBI SrfI SwaI