Restriction map for
pFN21AE9629
(Linear) (Six-base) MAPSORT of: KIEE9629.seq Check: 355 from: 1 to: 3003
KIEE9629.orf
With 182 enzymes: SgfI *
July 3, 2012 19:03 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 504 3003
Size: 504 2499
AccI GT'mk_AC
Cuts at: 0 380 3003
Size: 380 2623
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 245 661 1795 3003
Size: 245 416 1134 1208
Fragments arranged by size:
1208 1134 416 245
AflII C'TTAA_G
Cuts at: 0 1999 3003
Size: 1999 1004
AflIII A'CryG_T
Cuts at: 0 485 2761 3003
Size: 485 2276 242
Fragments arranged by size:
2276 485 242
AhdI GACnn_n'nnGTC
Cuts at: 0 156 2922 3003
Size: 156 2766 81
Fragments arranged by size:
2766 156 81
AleI CACnn'nnGTG
Cuts at: 0 1457 2642 2762 3003
Size: 1457 1185 120 241
Fragments arranged by size:
1457 1185 241 120
AlwNI CAG_nnn'CTG
Cuts at: 0 268 426 627 669 1296 2313 3003
Size: 268 158 201 42 627 1017 690
Fragments arranged by size:
1017 690 627 268 201 158 42
ApaI G_GGCC'C
Cuts at: 0 2266 2485 3003
Size: 2266 219 518
Fragments arranged by size:
2266 518 219
ApoI r'AATT_y
Cuts at: 0 196 214 372 844 1804 3003
Size: 196 18 158 472 960 1199
Fragments arranged by size:
1199 960 472 196 158 18
AvaI C'yCGr_G
Cuts at: 0 2900 3003
Size: 2900 103
AvrII C'CTAG_G
Cuts at: 0 2640 3003
Size: 2640 363
BamHI G'GATC_C
Cuts at: 0 915 1489 2633 3003
Size: 915 574 1144 370
Fragments arranged by size:
1144 915 574 370
BanI G'GyrC_C
Cuts at: 0 1685 2602 2747 3003
Size: 1685 917 145 256
Fragments arranged by size:
1685 917 256 145
BanII G_rGCy'C
Cuts at: 0 528 1961 2266 2485 2841 3003
Size: 528 1433 305 219 356 162
Fragments arranged by size:
1433 528 356 305 219 162
BbeI G_GCGC'C
Cuts at: 0 2606 2751 3003
Size: 2606 145 252
Fragments arranged by size:
2606 252 145
BbsI GAAGACnn'nnnn_
Cuts at: 0 56 3003
Size: 56 2947
BbvCI CC'TCA_GC
Cuts at: 0 2029 3003
Size: 2029 974
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 37 71 1552 1586 3003
Size: 37 34 1481 34 1417
Fragments arranged by size:
1481 1417 37 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 1451 3003
Size: 1451 1552
BclI T'GATC_A
Cuts at: 0 588 1449 3003
Size: 588 861 1554
Fragments arranged by size:
1554 861 588
BglI GCCn_nnn'nGGC
Cuts at: 0 778 2624 3003
Size: 778 1846 379
Fragments arranged by size:
1846 778 379
BglII A'GATC_T
Cuts at: 0 206 943 3003
Size: 206 737 2060
Fragments arranged by size:
2060 737 206
BlpI GC'TnA_GC
Cuts at: 0 2058 3003
Size: 2058 945
Bme1580I G_kGCm'C
Cuts at: 0 1688 1975 2266 2485 3003
Size: 1688 287 291 219 518
Fragments arranged by size:
1688 518 291 287 219
BmrI ACTGGGnnnn_n'
Cuts at: 0 754 1890 2375 3003
Size: 754 1136 485 628
Fragments arranged by size:
1136 754 628 485
BmtI G_CTAG'C
Cuts at: 0 2279 3003
Size: 2279 724
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 2073 3003
Size: 2073 930
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1476 2107 2212 2807 2855 2893 3003
Size: 1476 631 105 595 48 38 110
Fragments arranged by size:
1476 631 595 110 105 48 38
Bpu10I CC'TnA_GC
Cuts at: 0 1346 1382 2029 3003
Size: 1346 36 647 974
Fragments arranged by size:
1346 974 647 36
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 956 1015 1052 3003
Size: 956 59 37 1951
Fragments arranged by size:
1951 956 59 37
BsaI GGTCTCn'nnnn_
Cuts at: 0 1351 1525 1621 3003
Size: 1351 174 96 1382
Fragments arranged by size:
1382 1351 174 96
BsaAI yAC'GTr
Cuts at: 0 486 1650 3003
Size: 486 1164 1353
Fragments arranged by size:
1353 1164 486
BsaBI GATnn'nnATC
Cuts at: 0 224 1375 1439 3003
Size: 224 1151 64 1564
Fragments arranged by size:
1564 1151 224 64
BsaHI Gr'CG_yC
Cuts at: 0 2603 2748 3003
Size: 2603 145 255
Fragments arranged by size:
2603 255 145
BsaWI w'CCGG_w
Cuts at: 0 29 3003
Size: 29 2974
BseYI C'CCAG_C
Cuts at: 0 1163 1504 1925 1944 2342 3003
Size: 1163 341 421 19 398 661
Fragments arranged by size:
1163 661 421 398 341 19
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1849 2743 3003
Size: 1849 894 260
Fragments arranged by size:
1849 894 260
BsiEI CG_ry'CG
Cuts at: 0 168 3003
Size: 168 2835
BsiHKAI G_wGCw'C
Cuts at: 0 1622 3003
Size: 1622 1381
BsmI GAATG_Cn'
Cuts at: 0 1184 3003
Size: 1184 1819
Bsp1286I G_dGCh'C
Cuts at: 0 528 1622 1688 1961 1975 2266 2485 2841
Size: 528 1094 66 273 14 291 219 356
Cuts at: 2841 3003
Size: 162
Fragments arranged by size:
1094 528 356 291 273 219 162 66
14
BspHI T'CATG_A
Cuts at: 0 285 819 1409 3003
Size: 285 534 590 1594
Fragments arranged by size:
1594 590 534 285
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 161 504 1833 2443 3003
Size: 161 343 1329 610 560
Fragments arranged by size:
1329 610 560 343 161
BsrBI CCG'CTC
Cuts at: 0 762 1194 2821 3003
Size: 762 432 1627 182
Fragments arranged by size:
1627 762 432 182
BsrDI GCAATG_nn'
Cuts at: 0 1221 1568 3003
Size: 1221 347 1435
Fragments arranged by size:
1435 1221 347
BsrFI r'CCGG_y
Cuts at: 0 2495 3003
Size: 2495 508
BsrGI T'GTAC_A
Cuts at: 0 2138 3003
Size: 2138 865
BstAPI GCAn_nnn'nTGC
Cuts at: 0 519 1827 2687 2957 3003
Size: 519 1308 860 270 46
Fragments arranged by size:
1308 860 519 270 46
BstBI TT'CG_AA
Cuts at: 0 306 3003
Size: 306 2697
BstXI CCAn_nnnn'nTGG
Cuts at: 0 81 832 1512 1903 2190 2287 2644 2968
Size: 81 751 680 391 287 97 357 324
Cuts at: 2968 3003
Size: 35
Fragments arranged by size:
751 680 391 357 324 287 97 81
35
BstYI r'GATC_y
Cuts at: 0 206 915 943 1466 1489 2237 2633 3003
Size: 206 709 28 523 23 748 396 370
Fragments arranged by size:
748 709 523 396 370 206 28 23
Bsu36I CC'TnA_GG
Cuts at: 0 634 692 2230 3003
Size: 634 58 1538 773
Fragments arranged by size:
1538 773 634 58
BtgI C'CryG_G
Cuts at: 0 74 248 920 1401 1511 1721 2405 3003
Size: 74 174 672 481 110 210 684 598
Fragments arranged by size:
684 672 598 481 210 174 110 74
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 617 1362 3003
Size: 617 745 1641
Fragments arranged by size:
1641 745 617
BtsI GCAGTG_nn'
Cuts at: 0 2112 2676 2791 3003
Size: 2112 564 115 212
Fragments arranged by size:
2112 564 212 115
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 865 900 3003
Size: 865 35 2103
Fragments arranged by size:
2103 865 35
DraIII CAC_nnn'GTG
Cuts at: 0 2136 3003
Size: 2136 867
DrdI GACnn_nn'nnGTC
Cuts at: 0 2978 3003
Size: 2978 25
EaeI y'GGCC_r
Cuts at: 0 116 134 234 2289 2445 3003
Size: 116 18 100 2055 156 558
Fragments arranged by size:
2055 558 156 116 100 18
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 192 2228 3003
Size: 192 2036 775
Fragments arranged by size:
2036 775 192
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 245 661 1476 1795 2107 2212 2807 2855
Size: 245 416 815 319 312 105 595 48
Cuts at: 2855 2893 3003
Size: 38 110
Fragments arranged by size:
815 595 416 319 312 245 110 105
48 38
EcoNI CCTnn'n_nnAGG
Cuts at: 0 654 3003
Size: 654 2349
EcoO109I rG'GnC_Cy
Cuts at: 0 1169 2262 2263 2369 2481 2482 2572 2897
Size: 1169 1093 1 106 112 1 90 325
Cuts at: 2897 3003
Size: 106
Fragments arranged by size:
1169 1093 325 112 106 106 90 1
1
EcoRI G'AATT_C
Cuts at: 0 844 3003
Size: 844 2159
EcoRV GAT'ATC
Cuts at: 0 1938 3003
Size: 1938 1065
HaeII r_GCGC'y
Cuts at: 0 2606 2751 3003
Size: 2606 145 252
Fragments arranged by size:
2606 252 145
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 142 174 928 960 1140 1172 1474 1506
Size: 142 32 754 32 180 32 302 32
Cuts at: 1506 2172 2204 2908 2940 3003
Size: 666 32 704 32 63
Fragments arranged by size:
754 704 666 302 180 142 63 32
32 32 32 32 32
KasI G'GCGC_C
Cuts at: 0 2602 2747 3003
Size: 2602 145 256
Fragments arranged by size:
2602 256 145
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 2858 3003
Size: 2858 145
MscI TGG'CCA
Cuts at: 0 236 2447 3003
Size: 236 2211 556
Fragments arranged by size:
2211 556 236
MslI CAynn'nnrTG
Cuts at: 0 79 230 253 830 1457 2642 2762 3003
Size: 79 151 23 577 627 1185 120 241
Fragments arranged by size:
1185 627 577 241 151 120 79 23
MspA1I CmG'CkG
Cuts at: 0 728 2033 2062 3003
Size: 728 1305 29 941
Fragments arranged by size:
1305 941 728 29
NaeI GCC'GGC
Cuts at: 0 2497 3003
Size: 2497 506
NarI GG'CG_CC
Cuts at: 0 2603 2748 3003
Size: 2603 145 255
Fragments arranged by size:
2603 255 145
NcoI C'CATG_G
Cuts at: 0 74 248 920 1401 1721 3003
Size: 74 174 672 481 320 1282
Fragments arranged by size:
1282 672 481 320 174 74
NdeI CA'TA_TG
Cuts at: 0 1446 3003
Size: 1446 1557
NgoMIV G'CCGG_C
Cuts at: 0 2495 3003
Size: 2495 508
NheI G'CTAG_C
Cuts at: 0 2275 3003
Size: 2275 728
NspI r_CATG'y
Cuts at: 0 2765 3003
Size: 2765 238
PasI CC'CwG_GG
Cuts at: 0 1681 2270 2598 2610 2629 3003
Size: 1681 589 328 12 19 374
Fragments arranged by size:
1681 589 374 328 19 12
PciI A'CATG_T
Cuts at: 0 2761 3003
Size: 2761 242
PflMI CCAn_nnn'nTGG
Cuts at: 0 426 1216 1323 3003
Size: 426 790 107 1680
Fragments arranged by size:
1680 790 426 107
PfoI T'CCnGG_A
Cuts at: 0 2240 2559 2845 3003
Size: 2240 319 286 158
Fragments arranged by size:
2240 319 286 158
PmlI CAC'GTG
Cuts at: 0 486 3003
Size: 486 2517
PpuMI rG'GwC_Cy
Cuts at: 0 2369 3003
Size: 2369 634
PspOMI G'GGCC_C
Cuts at: 0 2262 2481 3003
Size: 2262 219 522
Fragments arranged by size:
2262 522 219
PstI C_TGCA'G
Cuts at: 0 515 2300 3003
Size: 515 1785 703
Fragments arranged by size:
1785 703 515
PvuII CAG'CTG
Cuts at: 0 728 2033 2062 3003
Size: 728 1305 29 941
Fragments arranged by size:
1305 941 728 29
SbfI CC_TGCA'GG
Cuts at: 0 2300 3003
Size: 2300 703
ScaI AGT'ACT
Cuts at: 0 361 1900 3003
Size: 361 1539 1103
Fragments arranged by size:
1539 1103 361
SfcI C'TryA_G
Cuts at: 0 511 2296 3003
Size: 511 1785 707
Fragments arranged by size:
1785 707 511
SfoI GGC'GCC
Cuts at: 0 2604 2749 3003
Size: 2604 145 254
Fragments arranged by size:
2604 254 145
SmaI CCC'GGG
Cuts at: 0 2902 3003
Size: 2902 101
SmlI C'TyrA_G
Cuts at: 0 971 994 1067 1999 3003
Size: 971 23 73 932 1004
Fragments arranged by size:
1004 971 932 73 23
StuI AGG'CCT
Cuts at: 0 1888 3003
Size: 1888 1115
StyI C'CwwG_G
Cuts at: 0 74 137 248 920 977 1401 1721 1889
Size: 74 63 111 672 57 424 320 168
Cuts at: 1889 2582 2640 3003
Size: 693 58 363
Fragments arranged by size:
693 672 424 363 320 168 111 74
63 58 57
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 595 1557 2418 3003
Size: 595 962 861 585
Fragments arranged by size:
962 861 595 585
TatI w'GTAC_w
Cuts at: 0 359 1898 2138 3003
Size: 359 1539 240 865
Fragments arranged by size:
1539 865 359 240
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 429 461 552 584 3003
Size: 429 32 91 32 2419
Fragments arranged by size:
2419 429 91 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 1993 2367 3003
Size: 1993 374 636
Fragments arranged by size:
1993 636 374
XbaI T'CTAG_A
Cuts at: 0 1188 3003
Size: 1188 1815
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 927 1451 3003
Size: 927 524 1552
Fragments arranged by size:
1552 927 524
XmaI C'CCGG_G
Cuts at: 0 2900 3003
Size: 2900 103
Enzymes that do cut:
AarI AccI AcuI AflII AflIII AhdI AleI AlwNI
ApaI ApoI AvaI AvrII BamHI BanI BanII BbeI
BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI
Bme1580I BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BstAPI BstBI BstXI BstYI Bsu36I BtgI BtgZI BtsI
CspCI DraIII DrdI EaeI EciI Eco57MI EcoNI EcoO109I
EcoRI EcoRV HaeII Hin4I KasI MmeI MscI MslI
MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NspI
PasI PciI PflMI PfoI PmlI PpuMI PspOMI PstI
PvuII SbfI ScaI SfcI SfoI SmaI SmlI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AatII Acc65I AclI AfeI AgeI AlfI AloI ApaLI
AscI AseI AsiSI BaeI BfrBI BmgBI BsaXI BseRI
BsiWI BsmBI BspEI BssHII BssSI BstEII BstZ17I ClaI
DraI EagI EarI EcoICRI FalI FseI FspI FspAI
HincII HindIII HpaI KpnI MfeI MluI NotI NruI
NsiI PacI PmeI PpiI PshAI PsiI PspXI PsrI
PvuI RsrII SacI SacII SalI SanDI SapI SexAI
SfiI SgfI SgrAI SnaBI SpeI SphI SrfI SspI
SwaI XhoI XmnI ZraI