Restriction map for
pFN21AE9628
(Linear) (Six-base) MAPSORT of: KIEE9628.seq Check: 550 from: 1 to: 2646
KIEE9628.orf
With 182 enzymes: SgfI *
July 3, 2012 19:03 ..
AccI GT'mk_AC
Cuts at: 0 127 2227 2646
Size: 127 2100 419
Fragments arranged by size:
2100 419 127
Acc65I G'GTAC_C
Cuts at: 0 772 2646
Size: 772 1874
AclI AA'CG_TT
Cuts at: 0 437 2646
Size: 437 2209
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1049 2646
Size: 1049 1597
AflIII A'CryG_T
Cuts at: 0 2513 2646
Size: 2513 133
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 950 982 2646
Size: 950 32 1664
Fragments arranged by size:
1664 950 32
ApoI r'AATT_y
Cuts at: 0 505 1312 1375 2646
Size: 505 807 63 1271
Fragments arranged by size:
1271 807 505 63
AseI AT'TA_AT
Cuts at: 0 952 2646
Size: 952 1694
AvaI C'yCGr_G
Cuts at: 0 26 1401 2646
Size: 26 1375 1245
Fragments arranged by size:
1375 1245 26
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 356 389 1779 1812 2646
Size: 356 33 1390 33 834
Fragments arranged by size:
1390 834 356 33 33
BanI G'GyrC_C
Cuts at: 0 66 772 2646
Size: 66 706 1874
Fragments arranged by size:
1874 706 66
BanII G_rGCy'C
Cuts at: 0 39 113 1911 2476 2646
Size: 39 74 1798 565 170
Fragments arranged by size:
1798 565 170 74 39
BbsI GAAGACnn'nnnn_
Cuts at: 0 1037 2646
Size: 1037 1609
BbvCI CC'TCA_GC
Cuts at: 0 1196 2646
Size: 1196 1450
BciVI GTATCCnnnnn_n'
Cuts at: 0 1753 2646
Size: 1753 893
BclI T'GATC_A
Cuts at: 0 489 2646
Size: 489 2157
BglII A'GATC_T
Cuts at: 0 2093 2646
Size: 2093 553
BmgBI CAC'GTC
Cuts at: 0 650 2646
Size: 650 1996
BmrI ACTGGGnnnn_n'
Cuts at: 0 151 2646
Size: 151 2495
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1927 2646
Size: 1927 719
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 2110 2327 2646
Size: 2110 217 319
Fragments arranged by size:
2110 319 217
Bpu10I CC'TnA_GC
Cuts at: 0 1196 2646
Size: 1196 1450
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 203 2490 2646
Size: 203 2287 156
Fragments arranged by size:
2287 203 156
BsaBI GATnn'nnATC
Cuts at: 0 467 1741 2646
Size: 467 1274 905
Fragments arranged by size:
1274 905 467
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 2232 2262 2646
Size: 2232 30 384
Fragments arranged by size:
2232 384 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 206 209 218 221 1919 2646
Size: 206 3 9 3 1698 727
Fragments arranged by size:
1698 727 206 9 3 3
BseYI C'CCAG_C
Cuts at: 0 247 1880 2646
Size: 247 1633 766
Fragments arranged by size:
1633 766 247
BsiEI CG_ry'CG
Cuts at: 0 139 2646
Size: 139 2507
BsiHKAI G_wGCw'C
Cuts at: 0 87 213 589 2646
Size: 87 126 376 2057
Fragments arranged by size:
2057 376 126 87
BsmBI CGTCTCn'nnnn_
Cuts at: 0 2638 2646
Size: 2638 8
Bsp1286I G_dGCh'C
Cuts at: 0 39 87 113 213 589 1911 2476 2646
Size: 39 48 26 100 376 1322 565 170
Fragments arranged by size:
1322 565 376 170 100 48 39 26
BsrBI CCG'CTC
Cuts at: 0 567 2646
Size: 567 2079
BsrDI GCAATG_nn'
Cuts at: 0 1517 2197 2646
Size: 1517 680 449
Fragments arranged by size:
1517 680 449
BssSI C'ACGA_G
Cuts at: 0 2584 2646
Size: 2584 62
BstYI r'GATC_y
Cuts at: 0 2093 2646
Size: 2093 553
Bsu36I CC'TnA_GG
Cuts at: 0 242 2646
Size: 242 2404
BtgI C'CryG_G
Cuts at: 0 1604 2646
Size: 1604 1042
BtsI GCAGTG_nn'
Cuts at: 0 1696 2646
Size: 1696 950
ClaI AT'CG_AT
Cuts at: 0 2140 2646
Size: 2140 506
DraIII CAC_nnn'GTG
Cuts at: 0 1174 2646
Size: 1174 1472
EaeI y'GGCC_r
Cuts at: 0 136 553 2646
Size: 136 417 2093
Fragments arranged by size:
2093 417 136
EagI C'GGCC_G
Cuts at: 0 136 2646
Size: 136 2510
EarI CTCTTCn'nnn_
Cuts at: 0 1401 2066 2646
Size: 1401 665 580
Fragments arranged by size:
1401 665 580
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1049 2110 2327 2646
Size: 1049 1061 217 319
Fragments arranged by size:
1061 1049 319 217
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1915 2646
Size: 1915 731
EcoO109I rG'GnC_Cy
Cuts at: 0 76 1155 2646
Size: 76 1079 1491
Fragments arranged by size:
1491 1079 76
EcoRI G'AATT_C
Cuts at: 0 1312 2646
Size: 1312 1334
EcoRV GAT'ATC
Cuts at: 0 404 1974 2646
Size: 404 1570 672
Fragments arranged by size:
1570 672 404
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 2218 2646
Size: 2218 428
HaeII r_GCGC'y
Cuts at: 0 265 519 2554 2646
Size: 265 254 2035 92
Fragments arranged by size:
2035 265 254 92
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 393 425 520 552 1642 1674 2646
Size: 393 32 95 32 1090 32 972
Fragments arranged by size:
1090 972 393 95 32 32 32
HincII GTy'rAC
Cuts at: 0 128 441 2228 2369 2627 2646
Size: 128 313 1787 141 258 19
Fragments arranged by size:
1787 313 258 141 128 19
KpnI G_GTAC'C
Cuts at: 0 776 2646
Size: 776 1870
MfeI C'AATT_G
Cuts at: 0 962 2646
Size: 962 1684
MslI CAynn'nnrTG
Cuts at: 0 1615 2278 2646
Size: 1615 663 368
Fragments arranged by size:
1615 663 368
MspA1I CmG'CkG
Cuts at: 0 141 1200 2646
Size: 141 1059 1446
Fragments arranged by size:
1446 1059 141
NcoI C'CATG_G
Cuts at: 0 1604 2646
Size: 1604 1042
NspI r_CATG'y
Cuts at: 0 337 2517 2646
Size: 337 2180 129
Fragments arranged by size:
2180 337 129
PciI A'CATG_T
Cuts at: 0 2513 2646
Size: 2513 133
PflMI CCAn_nnn'nTGG
Cuts at: 0 1824 2574 2646
Size: 1824 750 72
Fragments arranged by size:
1824 750 72
PfoI T'CCnGG_A
Cuts at: 0 2343 2646
Size: 2343 303
PpuMI rG'GwC_Cy
Cuts at: 0 76 2646
Size: 76 2570
PshAI GACnn'nnGTC
Cuts at: 0 1864 2646
Size: 1864 782
PsiI TTA'TAA
Cuts at: 0 2444 2522 2646
Size: 2444 78 124
Fragments arranged by size:
2444 124 78
PstI C_TGCA'G
Cuts at: 0 146 176 1703 2646
Size: 146 30 1527 943
Fragments arranged by size:
1527 943 146 30
PvuII CAG'CTG
Cuts at: 0 1200 2646
Size: 1200 1446
SalI G'TCGA_C
Cuts at: 0 126 2226 2646
Size: 126 2100 420
Fragments arranged by size:
2100 420 126
SanDI GG'GwC_CC
Cuts at: 0 76 2646
Size: 76 2570
SapI GCTCTTCn'nnn_
Cuts at: 0 2066 2646
Size: 2066 580
SexAI A'CCwGG_T
Cuts at: 0 302 2646
Size: 302 2344
SfcI C'TryA_G
Cuts at: 0 142 172 993 1080 1539 1585 1699 2646
Size: 142 30 821 87 459 46 114 947
Fragments arranged by size:
947 821 459 142 114 87 46 30
SmaI CCC'GGG
Cuts at: 0 1403 2646
Size: 1403 1243
SmlI C'TyrA_G
Cuts at: 0 218 2469 2646
Size: 218 2251 177
Fragments arranged by size:
2251 218 177
StyI C'CwwG_G
Cuts at: 0 1604 2646
Size: 1604 1042
TatI w'GTAC_w
Cuts at: 0 2484 2646
Size: 2484 162
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 2107 2139 2646
Size: 2107 32 507
Fragments arranged by size:
2107 507 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 526 1523 2646
Size: 526 997 1123
Fragments arranged by size:
1123 997 526
XmaI C'CCGG_G
Cuts at: 0 1401 2646
Size: 1401 1245
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AloI ApoI AseI
AvaI BaeI BanI BanII BbsI BbvCI BciVI BclI
BglII BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaBI
BsaXI BseRI BseYI BsiEI BsiHKAI BsmBI Bsp1286I BsrBI
BsrDI BssSI BstYI Bsu36I BtgI BtsI ClaI DraIII
EaeI EagI EarI Eco57MI EcoNI EcoO109I EcoRI EcoRV
FalI HaeII Hin4I HincII KpnI MfeI MslI MspA1I
NcoI NspI PciI PflMI PfoI PpuMI PshAI PsiI
PstI PvuII SalI SanDI SapI SexAI SfcI SmaI
SmlI StyI TatI TstI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AgeI AhdI AleI AlfI
AlwNI ApaI ApaLI AscI AsiSI AvrII BamHI BbeI
BcgI BfrBI BglI BlpI Bme1580I BmtI BsaI BsaAI
BsaHI BsaWI BsgI BsiWI BsmI BspEI BspHI BspMI
BsrFI BsrGI BssHII BstAPI BstBI BstEII BstXI BstZ17I
BtgZI CspCI DraI DrdI EciI EcoICRI FseI FspI
FspAI HindIII HpaI KasI MluI MmeI MscI NaeI
NarI NdeI NgoMIV NheI NotI NruI NsiI PacI
PasI PmeI PmlI PpiI PspOMI PspXI PsrI PvuI
RsrII SacI SacII SbfI ScaI SfiI SfoI SgfI
SgrAI SnaBI SpeI SphI SrfI SspI StuI SwaI
TaqII Tth111I XbaI XhoI XmnI ZraI