Restriction map for
pFN21AE9627
(Linear) (Six-base) MAPSORT of: KIEE9627.seq Check: 513 from: 1 to: 2583
KIEE9627.orf
With 182 enzymes: SgfI *
July 3, 2012 19:03 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 305 557 754 1034 1634 1888 2251 2428
Size: 305 252 197 280 600 254 363 177
Cuts at: 2428 2583
Size: 155
Fragments arranged by size:
600 363 305 280 254 252 197 177
155
AfeI AGC'GCT
Cuts at: 0 2428 2583
Size: 2428 155
AflII C'TTAA_G
Cuts at: 0 1623 2583
Size: 1623 960
AhdI GACnn_n'nnGTC
Cuts at: 0 368 2583
Size: 368 2215
AleI CACnn'nnGTG
Cuts at: 0 1391 2583
Size: 1391 1192
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 832 864 2583
Size: 832 32 1719
Fragments arranged by size:
1719 832 32
AlwNI CAG_nnn'CTG
Cuts at: 0 1135 2010 2583
Size: 1135 875 573
Fragments arranged by size:
1135 875 573
ApaI G_GGCC'C
Cuts at: 0 39 1114 2583
Size: 39 1075 1469
Fragments arranged by size:
1469 1075 39
ApaLI G'TGCA_C
Cuts at: 0 1451 2583
Size: 1451 1132
ApoI r'AATT_y
Cuts at: 0 669 880 1364 2583
Size: 669 211 484 1219
Fragments arranged by size:
1219 669 484 211
AseI AT'TA_AT
Cuts at: 0 1459 2583
Size: 1459 1124
AvaI C'yCGr_G
Cuts at: 0 14 2583
Size: 14 2569
AvrII C'CTAG_G
Cuts at: 0 103 2583
Size: 103 2480
BanII G_rGCy'C
Cuts at: 0 15 39 70 1114 1157 1847 2024 2583
Size: 15 24 31 1044 43 690 177 559
Fragments arranged by size:
1044 690 559 177 43 31 24 15
BbsI GAAGACnn'nnnn_
Cuts at: 0 435 2268 2583
Size: 435 1833 315
Fragments arranged by size:
1833 435 315
BbvCI CC'TCA_GC
Cuts at: 0 110 2583
Size: 110 2473
BglII A'GATC_T
Cuts at: 0 462 2583
Size: 462 2121
Bme1580I G_kGCm'C
Cuts at: 0 39 1114 1455 2583
Size: 39 1075 341 1128
Fragments arranged by size:
1128 1075 341 39
BmrI ACTGGGnnnn_n'
Cuts at: 0 14 369 978 1329 2583
Size: 14 355 609 351 1254
Fragments arranged by size:
1254 609 355 351 14
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1861 2583
Size: 1861 722
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 178 1223 1869 2276 2583
Size: 178 1045 646 407 307
Fragments arranged by size:
1045 646 407 307 178
Bpu10I CC'TnA_GC
Cuts at: 0 110 1151 2583
Size: 110 1041 1432
Fragments arranged by size:
1432 1041 110
BsaBI GATnn'nnATC
Cuts at: 0 580 1412 2583
Size: 580 832 1171
Fragments arranged by size:
1171 832 580
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 176 206 208 238 554 584 2583
Size: 176 30 2 30 316 30 1999
Fragments arranged by size:
1999 316 176 30 30 30 2
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 251 1853 2583
Size: 251 1602 730
Fragments arranged by size:
1602 730 251
BseYI C'CCAG_C
Cuts at: 0 1915 2080 2583
Size: 1915 165 503
Fragments arranged by size:
1915 503 165
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 780 2583
Size: 780 1803
BsiHKAI G_wGCw'C
Cuts at: 0 991 1157 1455 1847 2583
Size: 991 166 298 392 736
Fragments arranged by size:
991 736 392 298 166
BsmBI CGTCTCn'nnnn_
Cuts at: 0 2108 2583
Size: 2108 475
Bsp1286I G_dGCh'C
Cuts at: 0 15 39 70 991 1114 1157 1455 1847
Size: 15 24 31 921 123 43 298 392
Cuts at: 1847 2024 2583
Size: 177 559
Fragments arranged by size:
921 559 392 298 177 123 43 31
24 15
BspHI T'CATG_A
Cuts at: 0 258 665 2583
Size: 258 407 1918
Fragments arranged by size:
1918 407 258
BsrDI GCAATG_nn'
Cuts at: 0 1796 2073 2583
Size: 1796 277 510
Fragments arranged by size:
1796 510 277
BsrGI T'GTAC_A
Cuts at: 0 1726 2583
Size: 1726 857
BssSI C'ACGA_G
Cuts at: 0 1873 2583
Size: 1873 710
BstBI TT'CG_AA
Cuts at: 0 822 2583
Size: 822 1761
BstEII G'GTnAC_C
Cuts at: 0 2304 2583
Size: 2304 279
BstYI r'GATC_y
Cuts at: 0 462 2583
Size: 462 2121
Bsu36I CC'TnA_GG
Cuts at: 0 1770 2583
Size: 1770 813
BtgI C'CryG_G
Cuts at: 0 39 144 1562 2583
Size: 39 105 1418 1021
Fragments arranged by size:
1418 1021 105 39
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 2117 2583
Size: 2117 466
BtsI GCAGTG_nn'
Cuts at: 0 71 1123 2583
Size: 71 1052 1460
Fragments arranged by size:
1460 1052 71
ClaI AT'CG_AT
Cuts at: 0 1892 2583
Size: 1892 691
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 2141 2176 2583
Size: 2141 35 407
Fragments arranged by size:
2141 407 35
DraI TTT'AAA
Cuts at: 0 1548 2583
Size: 1548 1035
EaeI y'GGCC_r
Cuts at: 0 141 1793 2118 2464 2526 2583
Size: 141 1652 325 346 62 57
Fragments arranged by size:
1652 346 325 141 62 57
EarI CTCTTCn'nnn_
Cuts at: 0 449 2583
Size: 449 2134
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 1935 2583
Size: 1935 648
EcoICRI GAG'CTC
Cuts at: 0 1155 1845 2583
Size: 1155 690 738
Fragments arranged by size:
1155 738 690
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 178 305 557 754 1034 1223 1634 1869
Size: 178 127 252 197 280 189 411 235
Cuts at: 1869 1888 2251 2276 2428 2583
Size: 19 363 25 152 155
Fragments arranged by size:
411 363 280 252 235 197 189 178
155 152 127 25 19
EcoO109I rG'GnC_Cy
Cuts at: 0 35 1110 1111 1132 2583
Size: 35 1075 1 21 1451
Fragments arranged by size:
1451 1075 35 21 1
EcoRV GAT'ATC
Cuts at: 0 200 2583
Size: 200 2383
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1008 2583
Size: 1008 1575
HaeII r_GCGC'y
Cuts at: 0 2430 2583
Size: 2430 153
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 225 257 354 386 1524 1556 2583
Size: 225 32 97 32 1138 32 1027
Fragments arranged by size:
1138 1027 225 97 32 32 32
MfeI C'AATT_G
Cuts at: 0 713 2583
Size: 713 1870
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1364 2583
Size: 1364 1219
MscI TGG'CCA
Cuts at: 0 1795 2466 2528 2583
Size: 1795 671 62 55
Fragments arranged by size:
1795 671 62 55
MslI CAynn'nnrTG
Cuts at: 0 1391 1567 1573 2381 2583
Size: 1391 176 6 808 202
Fragments arranged by size:
1391 808 202 176 6
MspA1I CmG'CkG
Cuts at: 0 41 60 1915 1970 2124 2402 2583
Size: 41 19 1855 55 154 278 181
Fragments arranged by size:
1855 278 181 154 55 41 19
NcoI C'CATG_G
Cuts at: 0 1562 2583
Size: 1562 1021
NspI r_CATG'y
Cuts at: 0 1572 2079 2583
Size: 1572 507 504
Fragments arranged by size:
1572 507 504
PasI CC'CwG_GG
Cuts at: 0 1114 2583
Size: 1114 1469
PflMI CCAn_nnn'nTGG
Cuts at: 0 2281 2511 2583
Size: 2281 230 72
Fragments arranged by size:
2281 230 72
PfoI T'CCnGG_A
Cuts at: 0 2280 2583
Size: 2280 303
PsiI TTA'TAA
Cuts at: 0 698 2583
Size: 698 1885
PspOMI G'GGCC_C
Cuts at: 0 35 1110 2583
Size: 35 1075 1473
Fragments arranged by size:
1473 1075 35
PvuII CAG'CTG
Cuts at: 0 60 1915 2124 2583
Size: 60 1855 209 459
Fragments arranged by size:
1855 459 209 60
SacI G_AGCT'C
Cuts at: 0 1157 1847 2583
Size: 1157 690 736
Fragments arranged by size:
1157 736 690
SacII CC_GC'GG
Cuts at: 0 42 2583
Size: 42 2541
SapI GCTCTTCn'nnn_
Cuts at: 0 449 2583
Size: 449 2134
SexAI A'CCwGG_T
Cuts at: 0 87 2583
Size: 87 2496
SfcI C'TryA_G
Cuts at: 0 520 1035 1816 2583
Size: 520 515 781 767
Fragments arranged by size:
781 767 520 515
SmlI C'TyrA_G
Cuts at: 0 1623 2583
Size: 1623 960
SphI G_CATG'C
Cuts at: 0 1572 2583
Size: 1572 1011
SspI AAT'ATT
Cuts at: 0 639 2583
Size: 639 1944
StuI AGG'CCT
Cuts at: 0 108 2583
Size: 108 2475
StyI C'CwwG_G
Cuts at: 0 103 1389 1562 2583
Size: 103 1286 173 1021
Fragments arranged by size:
1286 1021 173 103
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1672 2583
Size: 1672 911
TatI w'GTAC_w
Cuts at: 0 1726 2072 2583
Size: 1726 346 511
Fragments arranged by size:
1726 511 346
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 176 208 554 586 2583
Size: 176 32 346 32 1997
Fragments arranged by size:
1997 346 176 32 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 202 1481 2583
Size: 202 1279 1102
Fragments arranged by size:
1279 1102 202
XmnI GAAnn'nnTTC
Cuts at: 0 1382 2583
Size: 1382 1201
Enzymes that do cut:
AcuI AfeI AflII AhdI AleI AloI AlwNI ApaI
ApaLI ApoI AseI AvaI AvrII BanII BbsI BbvCI
BglII Bme1580I BmrI BplI BpmI Bpu10I BsaBI BsaXI
BseRI BseYI BsgI BsiHKAI BsmBI Bsp1286I BspHI BsrDI
BsrGI BssSI BstBI BstEII BstYI Bsu36I BtgI BtgZI
BtsI ClaI CspCI DraI EaeI EarI EciI EcoICRI
Eco57MI EcoO109I EcoRV FalI HaeII Hin4I MfeI MmeI
MscI MslI MspA1I NcoI NspI PasI PflMI PfoI
PsiI PspOMI PvuII SacI SacII SapI SexAI SfcI
SmlI SphI SspI StuI StyI TaqII TatI TstI
XcmI XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AflIII AgeI AlfI
AscI AsiSI BaeI BamHI BanI BbeI BcgI BciVI
BclI BfrBI BglI BlpI BmgBI BmtI BpuEI BsaI
BsaAI BsaHI BsaWI BsiEI BsiWI BsmI BspEI BspMI
BsrBI BsrFI BssHII BstAPI BstXI BstZ17I DraIII DrdI
EagI EcoNI EcoRI FseI FspI FspAI HincII HindIII
HpaI KasI KpnI MluI NaeI NarI NdeI NgoMIV
NheI NotI NruI NsiI PacI PciI PmeI PmlI
PpiI PpuMI PshAI PspXI PsrI PstI PvuI RsrII
SalI SanDI SbfI ScaI SfiI SfoI SgfI SgrAI
SmaI SnaBI SpeI SrfI SwaI Tth111I XbaI XhoI
XmaI ZraI