Restriction map for
pFN21AE9626
(Linear) (Six-base) MAPSORT of: KIEE9626.seq Check: 9809 from: 1 to: 2580
KIEE9626.orf
With 182 enzymes: SgfI *
July 3, 2012 19:03 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1815 2580
Size: 1815 765
AccI GT'mk_AC
Cuts at: 0 137 246 949 2580
Size: 137 109 703 1631
Fragments arranged by size:
1631 703 137 109
Acc65I G'GTAC_C
Cuts at: 0 2275 2580
Size: 2275 305
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 940 982 2323 2498 2580
Size: 940 42 1341 175 82
Fragments arranged by size:
1341 940 175 82 42
AflIII A'CryG_T
Cuts at: 0 1233 1602 2580
Size: 1233 369 978
Fragments arranged by size:
1233 978 369
AleI CACnn'nnGTG
Cuts at: 0 529 2580
Size: 529 2051
AlwNI CAG_nnn'CTG
Cuts at: 0 1877 2580
Size: 1877 703
ApaI G_GGCC'C
Cuts at: 0 33 2580
Size: 33 2547
ApoI r'AATT_y
Cuts at: 0 376 772 2580
Size: 376 396 1808
Fragments arranged by size:
1808 396 376
AvaI C'yCGr_G
Cuts at: 0 386 2580
Size: 386 2194
AvrII C'CTAG_G
Cuts at: 0 175 2580
Size: 175 2405
BanI G'GyrC_C
Cuts at: 0 48 70 398 2275 2580
Size: 48 22 328 1877 305
Fragments arranged by size:
1877 328 305 48 22
BanII G_rGCy'C
Cuts at: 0 33 1511 2580
Size: 33 1478 1069
Fragments arranged by size:
1478 1069 33
BbsI GAAGACnn'nnnn_
Cuts at: 0 63 1582 2580
Size: 63 1519 998
Fragments arranged by size:
1519 998 63
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 643 677 2054 2088 2580
Size: 643 34 1377 34 492
Fragments arranged by size:
1377 643 492 34 34
BclI T'GATC_A
Cuts at: 0 1094 2580
Size: 1094 1486
BglII A'GATC_T
Cuts at: 0 1111 2580
Size: 1111 1469
Bme1580I G_kGCm'C
Cuts at: 0 33 53 2580
Size: 33 20 2527
Fragments arranged by size:
2527 33 20
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 539 1240 2580
Size: 539 701 1340
Fragments arranged by size:
1340 701 539
Bpu10I CC'TnA_GC
Cuts at: 0 437 2580
Size: 437 2143
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1510 2580
Size: 1510 1070
BsaI GGTCTCn'nnnn_
Cuts at: 0 233 1893 2580
Size: 233 1660 687
Fragments arranged by size:
1660 687 233
BsaAI yAC'GTr
Cuts at: 0 2373 2580
Size: 2373 207
BsaBI GATnn'nnATC
Cuts at: 0 761 1453 2580
Size: 761 692 1127
Fragments arranged by size:
1127 761 692
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1238 1268 1778 1808 1915 1945 2580
Size: 1238 30 510 30 107 30 635
Fragments arranged by size:
1238 635 510 107 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1661 2580
Size: 1661 919
BseYI C'CCAG_C
Cuts at: 0 25 32 2193 2580
Size: 25 7 2161 387
Fragments arranged by size:
2161 387 25 7
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1100 1407 2387 2580
Size: 1100 307 980 193
Fragments arranged by size:
1100 980 307 193
BsiWI C'GTAC_G
Cuts at: 0 2373 2580
Size: 2373 207
BsmBI CGTCTCn'nnnn_
Cuts at: 0 822 2580
Size: 822 1758
Bsp1286I G_dGCh'C
Cuts at: 0 33 53 1511 2580
Size: 33 20 1458 1069
Fragments arranged by size:
1458 1069 33 20
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1025 1815 2025 2409 2580
Size: 1025 790 210 384 171
Fragments arranged by size:
1025 790 384 210 171
BsrBI CCG'CTC
Cuts at: 0 553 2580
Size: 553 2027
BsrDI GCAATG_nn'
Cuts at: 0 2511 2580
Size: 2511 69
BsrGI T'GTAC_A
Cuts at: 0 2380 2538 2580
Size: 2380 158 42
Fragments arranged by size:
2380 158 42
BstAPI GCAn_nnn'nTGC
Cuts at: 0 405 1114 2580
Size: 405 709 1466
Fragments arranged by size:
1466 709 405
BstYI r'GATC_y
Cuts at: 0 1111 1173 2514 2580
Size: 1111 62 1341 66
Fragments arranged by size:
1341 1111 66 62
BstZ17I GTA'TAC
Cuts at: 0 950 2580
Size: 950 1630
BtgI C'CryG_G
Cuts at: 0 74 1296 1934 2580
Size: 74 1222 638 646
Fragments arranged by size:
1222 646 638 74
BtsI GCAGTG_nn'
Cuts at: 0 665 1390 2580
Size: 665 725 1190
Fragments arranged by size:
1190 725 665
ClaI AT'CG_AT
Cuts at: 0 653 2580
Size: 653 1927
DraI TTT'AAA
Cuts at: 0 222 1200 2580
Size: 222 978 1380
Fragments arranged by size:
1380 978 222
DraIII CAC_nnn'GTG
Cuts at: 0 1639 2580
Size: 1639 941
EaeI y'GGCC_r
Cuts at: 0 903 2580
Size: 903 1677
EarI CTCTTCn'nnn_
Cuts at: 0 959 2122 2580
Size: 959 1163 458
Fragments arranged by size:
1163 959 458
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 539 940 982 1240 2323 2498 2580
Size: 539 401 42 258 1083 175 82
Fragments arranged by size:
1083 539 401 258 175 82 42
EcoNI CCTnn'n_nnAGG
Cuts at: 0 145 338 578 2580
Size: 145 193 240 2002
Fragments arranged by size:
2002 240 193 145
EcoO109I rG'GnC_Cy
Cuts at: 0 19 29 2580
Size: 19 10 2551
Fragments arranged by size:
2551 19 10
EcoRV GAT'ATC
Cuts at: 0 2446 2580
Size: 2446 134
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 744 776 1328 1360 1778 1810 1898 1915
Size: 744 32 552 32 418 32 88 17
Cuts at: 1915 1930 1947 2580
Size: 15 17 633
Fragments arranged by size:
744 633 552 418 88 32 32 32
17 17 15
HincII GTy'rAC
Cuts at: 0 201 1970 2580
Size: 201 1769 610
Fragments arranged by size:
1769 610 201
KpnI G_GTAC'C
Cuts at: 0 2279 2580
Size: 2279 301
MluI A'CGCG_T
Cuts at: 0 1233 2580
Size: 1233 1347
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 2154 2580
Size: 2154 426
MslI CAynn'nnrTG
Cuts at: 0 529 2231 2297 2471 2580
Size: 529 1702 66 174 109
Fragments arranged by size:
1702 529 174 109 66
MspA1I CmG'CkG
Cuts at: 0 25 960 1422 2197 2343 2580
Size: 25 935 462 775 146 237
Fragments arranged by size:
935 775 462 237 146 25
NcoI C'CATG_G
Cuts at: 0 74 1296 1934 2580
Size: 74 1222 638 646
Fragments arranged by size:
1222 646 638 74
NdeI CA'TA_TG
Cuts at: 0 2232 2580
Size: 2232 348
NspI r_CATG'y
Cuts at: 0 1314 2580
Size: 1314 1266
PflMI CCAn_nnn'nTGG
Cuts at: 0 576 912 1810 1926 2580
Size: 576 336 898 116 654
Fragments arranged by size:
898 654 576 336 116
PfoI T'CCnGG_A
Cuts at: 0 1919 1940 2580
Size: 1919 21 640
Fragments arranged by size:
1919 640 21
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1498 1530 2580
Size: 1498 32 1050
Fragments arranged by size:
1498 1050 32
PpuMI rG'GwC_Cy
Cuts at: 0 19 2580
Size: 19 2561
PshAI GACnn'nnGTC
Cuts at: 0 170 2580
Size: 170 2410
PsiI TTA'TAA
Cuts at: 0 1964 2580
Size: 1964 616
PspOMI G'GGCC_C
Cuts at: 0 29 2580
Size: 29 2551
PstI C_TGCA'G
Cuts at: 0 2331 2580
Size: 2331 249
PvuII CAG'CTG
Cuts at: 0 960 1422 2197 2343 2580
Size: 960 462 775 146 237
Fragments arranged by size:
960 775 462 237 146
ScaI AGT'ACT
Cuts at: 0 416 2580
Size: 416 2164
SexAI A'CCwGG_T
Cuts at: 0 2423 2580
Size: 2423 157
SfcI C'TryA_G
Cuts at: 0 305 1546 2253 2327 2580
Size: 305 1241 707 74 253
Fragments arranged by size:
1241 707 305 253 74
SmlI C'TyrA_G
Cuts at: 0 1525 2580
Size: 1525 1055
SnaBI TAC'GTA
Cuts at: 0 2373 2580
Size: 2373 207
SspI AAT'ATT
Cuts at: 0 729 1038 1400 1731 2580
Size: 729 309 362 331 849
Fragments arranged by size:
849 729 362 331 309
StuI AGG'CCT
Cuts at: 0 864 2580
Size: 864 1716
StyI C'CwwG_G
Cuts at: 0 74 175 1296 1934 2531 2580
Size: 74 101 1121 638 597 49
Fragments arranged by size:
1121 638 597 101 74 49
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1880 2243 2580
Size: 1880 363 337
Fragments arranged by size:
1880 363 337
TatI w'GTAC_w
Cuts at: 0 414 2380 2538 2570 2580
Size: 414 1966 158 32 10
Fragments arranged by size:
1966 414 158 32 10
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 270 302 825 857 1238 1270 2580
Size: 270 32 523 32 381 32 1310
Fragments arranged by size:
1310 523 381 270 32 32 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 93 2580
Size: 93 2487
XmnI GAAnn'nnTTC
Cuts at: 0 1324 1408 2580
Size: 1324 84 1172
Fragments arranged by size:
1324 1172 84
Enzymes that do cut:
AarI AccI Acc65I AcuI AflIII AleI AlwNI ApaI
ApoI AvaI AvrII BanI BanII BbsI BcgI BclI
BglII Bme1580I BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaXI BseRI BseYI BsgI BsiWI BsmBI Bsp1286I BspMI
BsrBI BsrDI BsrGI BstAPI BstYI BstZ17I BtgI BtsI
ClaI DraI DraIII EaeI EarI Eco57MI EcoNI EcoO109I
EcoRV Hin4I HincII KpnI MluI MmeI MslI MspA1I
NcoI NdeI NspI PflMI PfoI PpiI PpuMI PshAI
PsiI PspOMI PstI PvuII ScaI SexAI SfcI SmlI
SnaBI SspI StuI StyI TaqII TatI TstI XcmI
XmnI
Enzymes that do not cut:
AatII AclI AfeI AflII AgeI AhdI AlfI AloI
ApaLI AscI AseI AsiSI BaeI BamHI BbeI BbvCI
BciVI BfrBI BglI BlpI BmgBI BmrI BmtI BplI
BsaHI BsaWI BsiEI BsiHKAI BsmI BspEI BspHI BsrFI
BssHII BssSI BstBI BstEII BstXI Bsu36I BtgZI CspCI
DrdI EagI EciI EcoICRI EcoRI FalI FseI FspI
FspAI HaeII HindIII HpaI KasI MfeI MscI NaeI
NarI NgoMIV NheI NotI NruI NsiI PacI PasI
PciI PmeI PmlI PspXI PsrI PvuI RsrII SacI
SacII SalI SanDI SapI SbfI SfiI SfoI SgfI
SgrAI SmaI SpeI SphI SrfI SwaI Tth111I XbaI
XhoI XmaI ZraI