Restriction map for
pFN21AE9622
(Linear) (Six-base) MAPSORT of: KIEE9622.seq Check: 3328 from: 1 to: 1683
KIEE9622.orf
With 182 enzymes: SgfI *
July 3, 2012 19:03 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1590 1683
Size: 1590 93
AccI GT'mk_AC
Cuts at: 0 538 1683
Size: 538 1145
AclI AA'CG_TT
Cuts at: 0 790 1683
Size: 790 893
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1112 1342 1683
Size: 1112 230 341
Fragments arranged by size:
1112 341 230
AflIII A'CryG_T
Cuts at: 0 277 1683
Size: 277 1406
ApaLI G'TGCA_C
Cuts at: 0 1431 1683
Size: 1431 252
ApoI r'AATT_y
Cuts at: 0 393 419 664 1515 1621 1683
Size: 393 26 245 851 106 62
Fragments arranged by size:
851 393 245 106 62 26
BanII G_rGCy'C
Cuts at: 0 1460 1569 1683
Size: 1460 109 114
Fragments arranged by size:
1460 114 109
BbsI GAAGACnn'nnnn_
Cuts at: 0 53 815 1374 1683
Size: 53 762 559 309
Fragments arranged by size:
762 559 309 53
BciVI GTATCCnnnnn_n'
Cuts at: 0 1358 1683
Size: 1358 325
BfrBI ATG'CAT
Cuts at: 0 292 1683
Size: 292 1391
Bme1580I G_kGCm'C
Cuts at: 0 1435 1683
Size: 1435 248
BmrI ACTGGGnnnn_n'
Cuts at: 0 514 1683
Size: 514 1169
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 372 1591 1683
Size: 372 1219 92
Fragments arranged by size:
1219 372 92
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 479 1683
Size: 479 1204
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1257 1287 1683
Size: 1257 30 396
Fragments arranged by size:
1257 396 30
BseYI C'CCAG_C
Cuts at: 0 1404 1683
Size: 1404 279
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 107 1683
Size: 107 1576
BsiHKAI G_wGCw'C
Cuts at: 0 1224 1435 1683
Size: 1224 211 248
Fragments arranged by size:
1224 248 211
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1240 1542 1683
Size: 1240 302 141
Fragments arranged by size:
1240 302 141
Bsp1286I G_dGCh'C
Cuts at: 0 1224 1435 1460 1569 1683
Size: 1224 211 25 109 114
Fragments arranged by size:
1224 211 114 109 25
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 98 339 1590 1683
Size: 98 241 1251 93
Fragments arranged by size:
1251 241 98 93
BsrDI GCAATG_nn'
Cuts at: 0 1065 1683
Size: 1065 618
BsrGI T'GTAC_A
Cuts at: 0 720 1683
Size: 720 963
BssSI C'ACGA_G
Cuts at: 0 1176 1683
Size: 1176 507
BstBI TT'CG_AA
Cuts at: 0 668 1471 1683
Size: 668 803 212
Fragments arranged by size:
803 668 212
BstXI CCAn_nnnn'nTGG
Cuts at: 0 645 1683
Size: 645 1038
BtsI GCAGTG_nn'
Cuts at: 0 154 1415 1683
Size: 154 1261 268
Fragments arranged by size:
1261 268 154
DraI TTT'AAA
Cuts at: 0 584 1683
Size: 584 1099
EaeI y'GGCC_r
Cuts at: 0 2 1683
Size: 2 1681
EarI CTCTTCn'nnn_
Cuts at: 0 872 1013 1476 1683
Size: 872 141 463 207
Fragments arranged by size:
872 463 207 141
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 372 1112 1342 1591 1683
Size: 372 740 230 249 92
Fragments arranged by size:
740 372 249 230 92
EcoO109I rG'GnC_Cy
Cuts at: 0 1488 1683
Size: 1488 195
EcoRI G'AATT_C
Cuts at: 0 664 1515 1683
Size: 664 851 168
Fragments arranged by size:
851 664 168
EcoRV GAT'ATC
Cuts at: 0 240 1329 1683
Size: 240 1089 354
Fragments arranged by size:
1089 354 240
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 58 322 1683
Size: 58 264 1361
Fragments arranged by size:
1361 264 58
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 370 402 1683
Size: 370 32 1281
Fragments arranged by size:
1281 370 32
HincII GTy'rAC
Cuts at: 0 759 1683
Size: 759 924
HpaI GTT'AAC
Cuts at: 0 759 1683
Size: 759 924
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 657 1068 1683
Size: 657 411 615
Fragments arranged by size:
657 615 411
MscI TGG'CCA
Cuts at: 0 4 1683
Size: 4 1679
MspA1I CmG'CkG
Cuts at: 0 252 1683
Size: 252 1431
NsiI A_TGCA'T
Cuts at: 0 294 1683
Size: 294 1389
NspI r_CATG'y
Cuts at: 0 281 705 1683
Size: 281 424 978
Fragments arranged by size:
978 424 281
PciI A'CATG_T
Cuts at: 0 277 1683
Size: 277 1406
PfoI T'CCnGG_A
Cuts at: 0 444 1683
Size: 444 1239
PpuMI rG'GwC_Cy
Cuts at: 0 1488 1683
Size: 1488 195
PvuII CAG'CTG
Cuts at: 0 252 1683
Size: 252 1431
SanDI GG'GwC_CC
Cuts at: 0 1488 1683
Size: 1488 195
SfcI C'TryA_G
Cuts at: 0 247 1644 1683
Size: 247 1397 39
Fragments arranged by size:
1397 247 39
SmlI C'TyrA_G
Cuts at: 0 494 1677 1683
Size: 494 1183 6
Fragments arranged by size:
1183 494 6
SpeI A'CTAG_T
Cuts at: 0 693 1683
Size: 693 990
StuI AGG'CCT
Cuts at: 0 1206 1683
Size: 1206 477
StyI C'CwwG_G
Cuts at: 0 5 1683
Size: 5 1678
TatI w'GTAC_w
Cuts at: 0 720 1160 1683
Size: 720 440 523
Fragments arranged by size:
720 523 440
XmnI GAAnn'nnTTC
Cuts at: 0 461 1371 1683
Size: 461 910 312
Fragments arranged by size:
910 461 312
Enzymes that do cut:
AarI AccI AclI AcuI AflIII ApaLI ApoI BanII
BbsI BciVI BfrBI Bme1580I BmrI BpmI BpuEI BsaXI
BseYI BsgI BsiHKAI BsmBI Bsp1286I BspMI BsrDI BsrGI
BssSI BstBI BstXI BtsI DraI EaeI EarI Eco57MI
EcoO109I EcoRI EcoRV FalI Hin4I HincII HpaI MmeI
MscI MspA1I NsiI NspI PciI PfoI PpuMI PvuII
SanDI SfcI SmlI SpeI StuI StyI TatI XmnI
Enzymes that do not cut:
AatII Acc65I AfeI AflII AgeI AhdI AleI AlfI
AloI AlwNI ApaI AscI AseI AsiSI AvaI AvrII
BaeI BamHI BanI BbeI BbvCI BcgI BclI BglI
BglII BlpI BmgBI BmtI BplI Bpu10I BsaI BsaAI
BsaBI BsaHI BsaWI BseRI BsiEI BsiWI BsmI BspEI
BspHI BsrBI BsrFI BssHII BstAPI BstEII BstYI BstZ17I
Bsu36I BtgI BtgZI ClaI CspCI DraIII DrdI EagI
EciI EcoICRI EcoNI FseI FspI FspAI HaeII HindIII
KasI KpnI MfeI MluI MslI NaeI NarI NcoI
NdeI NgoMIV NheI NotI NruI PacI PasI PflMI
PmeI PmlI PpiI PshAI PsiI PspOMI PspXI PsrI
PstI PvuI RsrII SacI SacII SalI SapI SbfI
ScaI SexAI SfiI SfoI SgfI SgrAI SmaI SnaBI
SphI SrfI SspI SwaI TaqII TstI Tth111I XbaI
XcmI XhoI XmaI ZraI