Restriction map for
pFN21AE9616
(Linear) (Six-base) MAPSORT of: KIEE9616.seq Check: 8827 from: 1 to: 1068
KIEE9616.orf
With 182 enzymes: SgfI *
March 22, 2012 18:34 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1041 1068
Size: 1041 27
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 202 459 536 1000 1068
Size: 202 257 77 464 68
Fragments arranged by size:
464 257 202 77 68
ApoI r'AATT_y
Cuts at: 0 155 1068
Size: 155 913
BanII G_rGCy'C
Cuts at: 0 359 1068
Size: 359 709
BbsI GAAGACnn'nnnn_
Cuts at: 0 776 1068
Size: 776 292
BclI T'GATC_A
Cuts at: 0 63 1068
Size: 63 1005
BlpI GC'TnA_GC
Cuts at: 0 358 1068
Size: 358 710
BmrI ACTGGGnnnn_n'
Cuts at: 0 924 1068
Size: 924 144
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 145 1068
Size: 145 923
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 230 1068
Size: 230 838
BsaBI GATnn'nnATC
Cuts at: 0 1050 1056 1068
Size: 1050 6 12
Fragments arranged by size:
1050 12 6
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 233 1068
Size: 233 835
BsiHKAI G_wGCw'C
Cuts at: 0 359 1068
Size: 359 709
Bsp1286I G_dGCh'C
Cuts at: 0 359 1068
Size: 359 709
BspHI T'CATG_A
Cuts at: 0 60 1068
Size: 60 1008
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1041 1068
Size: 1041 27
BsrFI r'CCGG_y
Cuts at: 0 314 362 1068
Size: 314 48 706
Fragments arranged by size:
706 314 48
BstYI r'GATC_y
Cuts at: 0 984 1045 1051 1057 1068
Size: 984 61 6 6 11
Fragments arranged by size:
984 61 11 6 6
Bsu36I CC'TnA_GG
Cuts at: 0 457 1068
Size: 457 611
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 333 1068
Size: 333 735
EcoICRI GAG'CTC
Cuts at: 0 357 1068
Size: 357 711
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 145 202 459 536 1000 1068
Size: 145 57 257 77 464 68
Fragments arranged by size:
464 257 145 77 68 57
EcoNI CCTnn'n_nnAGG
Cuts at: 0 179 1068
Size: 179 889
EcoRI G'AATT_C
Cuts at: 0 155 1068
Size: 155 913
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 8 40 48 80 660 692 703 735
Size: 8 32 8 32 580 32 11 32
Cuts at: 735 1068
Size: 333
Fragments arranged by size:
580 333 32 32 32 32 11 8
8
HindIII A'AGCT_T
Cuts at: 0 1012 1068
Size: 1012 56
MfeI C'AATT_G
Cuts at: 0 70 1068
Size: 70 998
MspA1I CmG'CkG
Cuts at: 0 903 1068
Size: 903 165
NspI r_CATG'y
Cuts at: 0 975 1068
Size: 975 93
PshAI GACnn'nnGTC
Cuts at: 0 219 1068
Size: 219 849
PstI C_TGCA'G
Cuts at: 0 908 1068
Size: 908 160
PvuII CAG'CTG
Cuts at: 0 903 1068
Size: 903 165
SacI G_AGCT'C
Cuts at: 0 359 1068
Size: 359 709
SfcI C'TryA_G
Cuts at: 0 904 1021 1068
Size: 904 117 47
Fragments arranged by size:
904 117 47
SmlI C'TyrA_G
Cuts at: 0 245 1068
Size: 245 823
SphI G_CATG'C
Cuts at: 0 975 1068
Size: 975 93
SspI AAT'ATT
Cuts at: 0 830 1068
Size: 830 238
StuI AGG'CCT
Cuts at: 0 910 1068
Size: 910 158
StyI C'CwwG_G
Cuts at: 0 162 1068
Size: 162 906
TatI w'GTAC_w
Cuts at: 0 951 1068
Size: 951 117
XmnI GAAnn'nnTTC
Cuts at: 0 155 215 1068
Size: 155 60 853
Fragments arranged by size:
853 155 60
Enzymes that do cut:
AarI AcuI ApoI BanII BbsI BclI BlpI BmrI
BpmI BpuEI BsaBI BseRI BsiHKAI Bsp1286I BspHI BspMI
BsrFI BstYI Bsu36I EciI EcoICRI Eco57MI EcoNI EcoRI
Hin4I HindIII MfeI MspA1I NspI PshAI PstI PvuII
SacI SfcI SmlI SphI SspI StuI StyI TatI
XmnI
Enzymes that do not cut:
AatII AccI Acc65I AclI AfeI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaI ApaLI AscI
AseI AsiSI AvaI AvrII BaeI BamHI BanI BbeI
BbvCI BcgI BciVI BfrBI BglI BglII Bme1580I BmgBI
BmtI BplI Bpu10I BsaI BsaAI BsaHI BsaWI BsaXI
BseYI BsgI BsiEI BsiWI BsmI BsmBI BspEI BsrBI
BsrDI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstZ17I BtgI BtgZI BtsI ClaI CspCI DraI DraIII
DrdI EaeI EagI EarI EcoO109I EcoRV FalI FseI
FspI FspAI HaeII HincII HpaI KasI KpnI MluI
MmeI MscI MslI NaeI NarI NcoI NdeI NgoMIV
NheI NotI NruI NsiI PacI PasI PciI PflMI
PfoI PmeI PmlI PpiI PpuMI PsiI PspOMI PspXI
PsrI PvuI RsrII SacII SalI SanDI SapI SbfI
ScaI SexAI SfiI SfoI SgfI SgrAI SmaI SnaBI
SpeI SrfI SwaI TaqII TstI Tth111I XbaI XcmI
XhoI XmaI ZraI