Restriction map for
pFN21AE9615
(Linear) (Six-base) MAPSORT of: KIEE9615.seq Check: 2432 from: 1 to: 1044
KIEE9615.orf
With 182 enzymes: SgfI *
July 3, 2012 19:03 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 921 1044
Size: 921 123
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 106 709 1044
Size: 106 603 335
Fragments arranged by size:
603 335 106
AleI CACnn'nnGTG
Cuts at: 0 979 1044
Size: 979 65
AlwNI CAG_nnn'CTG
Cuts at: 0 669 1044
Size: 669 375
ApaI G_GGCC'C
Cuts at: 0 676 1044
Size: 676 368
ApaLI G'TGCA_C
Cuts at: 0 244 1044
Size: 244 800
AvaI C'yCGr_G
Cuts at: 0 129 209 1044
Size: 129 80 835
Fragments arranged by size:
835 129 80
BamHI G'GATC_C
Cuts at: 0 51 1044
Size: 51 993
BanI G'GyrC_C
Cuts at: 0 93 1044
Size: 93 951
BanII G_rGCy'C
Cuts at: 0 396 676 866 1044
Size: 396 280 190 178
Fragments arranged by size:
396 280 190 178
BbsI GAAGACnn'nnnn_
Cuts at: 0 114 716 1044
Size: 114 602 328
Fragments arranged by size:
602 328 114
BglI GCCn_nnn'nGGC
Cuts at: 0 238 1044
Size: 238 806
BglII A'GATC_T
Cuts at: 0 875 1044
Size: 875 169
Bme1580I G_kGCm'C
Cuts at: 0 248 462 676 1044
Size: 248 214 214 368
Fragments arranged by size:
368 248 214 214
BmrI ACTGGGnnnn_n'
Cuts at: 0 477 494 1044
Size: 477 17 550
Fragments arranged by size:
550 477 17
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 44 1044
Size: 44 1000
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 789 1044
Size: 789 255
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 325 1044
Size: 325 719
BsaI GGTCTCn'nnnn_
Cuts at: 0 765 1044
Size: 765 279
BsaBI GATnn'nnATC
Cuts at: 0 880 1044
Size: 880 164
BsaHI Gr'CG_yC
Cuts at: 0 119 1044
Size: 119 925
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 367 397 1044
Size: 367 30 647
Fragments arranged by size:
647 367 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 658 1044
Size: 658 386
BseYI C'CCAG_C
Cuts at: 0 261 396 1044
Size: 261 135 648
Fragments arranged by size:
648 261 135
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 67 136 166 298 313 1044
Size: 67 69 30 132 15 731
Fragments arranged by size:
731 132 69 67 30 15
BsiHKAI G_wGCw'C
Cuts at: 0 248 1044
Size: 248 796
Bsp1286I G_dGCh'C
Cuts at: 0 248 396 462 676 866 1044
Size: 248 148 66 214 190 178
Fragments arranged by size:
248 214 190 178 148 66
BspHI T'CATG_A
Cuts at: 0 317 1044
Size: 317 727
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 921 1044
Size: 921 123
BsrBI CCG'CTC
Cuts at: 0 270 456 915 1044
Size: 270 186 459 129
Fragments arranged by size:
459 270 186 129
BsrDI GCAATG_nn'
Cuts at: 0 306 1044
Size: 306 738
BsrFI r'CCGG_y
Cuts at: 0 90 1044
Size: 90 954
BsrGI T'GTAC_A
Cuts at: 0 2 1044
Size: 2 1042
BstXI CCAn_nnnn'nTGG
Cuts at: 0 355 552 1044
Size: 355 197 492
Fragments arranged by size:
492 355 197
BstYI r'GATC_y
Cuts at: 0 51 875 1044
Size: 51 824 169
Fragments arranged by size:
824 169 51
Bsu36I CC'TnA_GG
Cuts at: 0 704 995 1044
Size: 704 291 49
Fragments arranged by size:
704 291 49
BtgI C'CryG_G
Cuts at: 0 331 1044
Size: 331 713
EaeI y'GGCC_r
Cuts at: 0 584 935 1044
Size: 584 351 109
Fragments arranged by size:
584 351 109
EarI CTCTTCn'nnn_
Cuts at: 0 161 319 1044
Size: 161 158 725
Fragments arranged by size:
725 161 158
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 106 709 789 1044
Size: 106 603 80 255
Fragments arranged by size:
603 255 106 80
EcoNI CCTnn'n_nnAGG
Cuts at: 0 790 1044
Size: 790 254
EcoO109I rG'GnC_Cy
Cuts at: 0 224 336 573 666 673 1044
Size: 224 112 237 93 7 371
Fragments arranged by size:
371 237 224 112 93 7
EcoRV GAT'ATC
Cuts at: 0 833 1044
Size: 833 211
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 978 1010 1044
Size: 978 32 34
Fragments arranged by size:
978 34 32
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 569 1044
Size: 569 475
MscI TGG'CCA
Cuts at: 0 586 1044
Size: 586 458
MslI CAynn'nnrTG
Cuts at: 0 979 1044
Size: 979 65
MspA1I CmG'CkG
Cuts at: 0 400 721 1044
Size: 400 321 323
Fragments arranged by size:
400 323 321
NspI r_CATG'y
Cuts at: 0 67 960 1044
Size: 67 893 84
Fragments arranged by size:
893 84 67
PflMI CCAn_nnn'nTGG
Cuts at: 0 669 1044
Size: 669 375
PfoI T'CCnGG_A
Cuts at: 0 500 551 590 1044
Size: 500 51 39 454
Fragments arranged by size:
500 454 51 39
PpuMI rG'GwC_Cy
Cuts at: 0 573 666 1044
Size: 573 93 378
Fragments arranged by size:
573 378 93
PspOMI G'GGCC_C
Cuts at: 0 672 1044
Size: 672 372
PstI C_TGCA'G
Cuts at: 0 932 1044
Size: 932 112
PvuII CAG'CTG
Cuts at: 0 400 721 1044
Size: 400 321 323
Fragments arranged by size:
400 323 321
SbfI CC_TGCA'GG
Cuts at: 0 932 1044
Size: 932 112
SexAI A'CCwGG_T
Cuts at: 0 419 1044
Size: 419 625
SfcI C'TryA_G
Cuts at: 0 928 1044
Size: 928 116
SmaI CCC'GGG
Cuts at: 0 211 1044
Size: 211 833
SmlI C'TyrA_G
Cuts at: 0 340 1044
Size: 340 704
SphI G_CATG'C
Cuts at: 0 960 1044
Size: 960 84
StuI AGG'CCT
Cuts at: 0 59 1044
Size: 59 985
StyI C'CwwG_G
Cuts at: 0 791 1044
Size: 791 253
TatI w'GTAC_w
Cuts at: 0 2 1044
Size: 2 1042
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 29 367 399 1044
Size: 29 338 32 645
Fragments arranged by size:
645 338 32 29
XmaI C'CCGG_G
Cuts at: 0 209 1044
Size: 209 835
XmnI GAAnn'nnTTC
Cuts at: 0 520 1044
Size: 520 524
Enzymes that do cut:
AarI AcuI AleI AlwNI ApaI ApaLI AvaI BamHI
BanI BanII BbsI BglI BglII Bme1580I BmrI BplI
BpmI BpuEI BsaI BsaBI BsaHI BsaXI BseRI BseYI
BsgI BsiHKAI Bsp1286I BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BstXI BstYI Bsu36I BtgI EaeI EarI Eco57MI
EcoNI EcoO109I EcoRV Hin4I MmeI MscI MslI MspA1I
NspI PflMI PfoI PpuMI PspOMI PstI PvuII SbfI
SexAI SfcI SmaI SmlI SphI StuI StyI TatI
TstI XmaI XmnI
Enzymes that do not cut:
AatII AccI Acc65I AclI AfeI AflII AflIII AgeI
AhdI AlfI AloI ApoI AscI AseI AsiSI AvrII
BaeI BbeI BbvCI BcgI BciVI BclI BfrBI BlpI
BmgBI BmtI Bpu10I BsaAI BsaWI BsiEI BsiWI BsmI
BsmBI BspEI BssHII BssSI BstAPI BstBI BstEII BstZ17I
BtgZI BtsI ClaI CspCI DraI DraIII DrdI EagI
EciI EcoICRI EcoRI FalI FseI FspI FspAI HaeII
HincII HindIII HpaI KasI KpnI MfeI MluI NaeI
NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI
PacI PasI PciI PmeI PmlI PpiI PshAI PsiI
PspXI PsrI PvuI RsrII SacI SacII SalI SanDI
SapI ScaI SfiI SfoI SgfI SgrAI SnaBI SpeI
SrfI SspI SwaI TaqII Tth111I XbaI XcmI XhoI
ZraI