Restriction map for
pFN21AE9609
(Linear) (Six-base) MAPSORT of: KIEE9609.seq Check: 4557 from: 1 to: 831
KIEE9609.orf
With 182 enzymes: SgfI *
March 22, 2012 18:33 ..
AccI GT'mk_AC
Cuts at: 0 649 801 831
Size: 649 152 30
Fragments arranged by size:
649 152 30
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 154 263 316 604 831
Size: 154 109 53 288 227
Fragments arranged by size:
288 227 154 109 53
AlwNI CAG_nnn'CTG
Cuts at: 0 498 645 831
Size: 498 147 186
Fragments arranged by size:
498 186 147
ApoI r'AATT_y
Cuts at: 0 822 831
Size: 822 9
BbsI GAAGACnn'nnnn_
Cuts at: 0 251 831
Size: 251 580
BbvCI CC'TCA_GC
Cuts at: 0 50 683 689 831
Size: 50 633 6 142
Fragments arranged by size:
633 142 50 6
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 712 746 831
Size: 712 34 85
Fragments arranged by size:
712 85 34
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 397 622 831
Size: 397 225 209
Fragments arranged by size:
397 225 209
Bpu10I CC'TnA_GC
Cuts at: 0 50 203 218 275 683 689 831
Size: 50 153 15 57 408 6 142
Fragments arranged by size:
408 153 142 57 50 15 6
BsaI GGTCTCn'nnnn_
Cuts at: 0 229 764 831
Size: 229 535 67
Fragments arranged by size:
535 229 67
BsaAI yAC'GTr
Cuts at: 0 318 831
Size: 318 513
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 103 831
Size: 103 728
BseYI C'CCAG_C
Cuts at: 0 719 831
Size: 719 112
BsiHKAI G_wGCw'C
Cuts at: 0 317 831
Size: 317 514
Bsp1286I G_dGCh'C
Cuts at: 0 317 831
Size: 317 514
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 816 831
Size: 816 15
BsrBI CCG'CTC
Cuts at: 0 606 831
Size: 606 225
BssSI C'ACGA_G
Cuts at: 0 249 831
Size: 249 582
BstXI CCAn_nnnn'nTGG
Cuts at: 0 114 831
Size: 114 717
BstYI r'GATC_y
Cuts at: 0 110 831
Size: 110 721
BstZ17I GTA'TAC
Cuts at: 0 650 831
Size: 650 181
Bsu36I CC'TnA_GG
Cuts at: 0 677 831
Size: 677 154
BtgI C'CryG_G
Cuts at: 0 434 761 831
Size: 434 327 70
Fragments arranged by size:
434 327 70
BtsI GCAGTG_nn'
Cuts at: 0 212 831
Size: 212 619
EaeI y'GGCC_r
Cuts at: 0 431 831
Size: 431 400
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 37 517 831
Size: 37 480 314
Fragments arranged by size:
480 314 37
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 154 263 316 397 604 622 831
Size: 154 109 53 81 207 18 209
Fragments arranged by size:
209 207 154 109 81 53 18
EcoNI CCTnn'n_nnAGG
Cuts at: 0 49 85 202 343 831
Size: 49 36 117 141 488
Fragments arranged by size:
488 141 117 49 36
EcoO109I rG'GnC_Cy
Cuts at: 0 42 348 831
Size: 42 306 483
Fragments arranged by size:
483 306 42
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 525 557 831
Size: 525 32 274
Fragments arranged by size:
525 274 32
MfeI C'AATT_G
Cuts at: 0 266 831
Size: 266 565
MscI TGG'CCA
Cuts at: 0 433 831
Size: 433 398
MspA1I CmG'CkG
Cuts at: 0 10 831
Size: 10 821
NcoI C'CATG_G
Cuts at: 0 434 761 831
Size: 434 327 70
Fragments arranged by size:
434 327 70
PfoI T'CCnGG_A
Cuts at: 0 392 831
Size: 392 439
PpuMI rG'GwC_Cy
Cuts at: 0 42 831
Size: 42 789
PstI C_TGCA'G
Cuts at: 0 33 86 163 191 494 516 620 659
Size: 33 53 77 28 303 22 104 39
Cuts at: 659 831
Size: 172
Fragments arranged by size:
303 172 104 77 53 39 33 28
22
SbfI CC_TGCA'GG
Cuts at: 0 86 659 831
Size: 86 573 172
Fragments arranged by size:
573 172 86
SfcI C'TryA_G
Cuts at: 0 29 74 82 159 187 306 490 512
Size: 29 45 8 77 28 119 184 22
Cuts at: 512 616 655 831
Size: 104 39 176
Fragments arranged by size:
184 176 119 104 77 45 39 29
28 22 8
StuI AGG'CCT
Cuts at: 0 80 831
Size: 80 751
StyI C'CwwG_G
Cuts at: 0 233 344 434 761 831
Size: 233 111 90 327 70
Fragments arranged by size:
327 233 111 90 70
TatI w'GTAC_w
Cuts at: 0 748 831
Size: 748 83
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 426 831
Size: 426 405
Enzymes that do cut:
AccI AcuI AlwNI ApoI BbsI BbvCI BcgI BpmI
Bpu10I BsaI BsaAI BseRI BseYI BsiHKAI Bsp1286I BspMI
BsrBI BssSI BstXI BstYI BstZ17I Bsu36I BtgI BtsI
EaeI EciI Eco57MI EcoNI EcoO109I Hin4I MfeI MscI
MspA1I NcoI PfoI PpuMI PstI SbfI SfcI StuI
StyI TatI XcmI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AflII AflIII AgeI
AhdI AleI AlfI AloI ApaI ApaLI AscI AseI
AsiSI AvaI AvrII BaeI BamHI BanI BanII BbeI
BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpuEI BsaBI BsaHI BsaWI BsaXI
BsgI BsiEI BsiWI BsmI BsmBI BspEI BspHI BsrDI
BsrFI BsrGI BssHII BstAPI BstBI BstEII BtgZI ClaI
CspCI DraI DraIII DrdI EagI EarI EcoICRI EcoRI
EcoRV FalI FseI FspI FspAI HaeII HincII HindIII
HpaI KasI KpnI MluI MmeI MslI NaeI NarI
NdeI NgoMIV NheI NotI NruI NsiI NspI PacI
PasI PciI PflMI PmeI PmlI PpiI PshAI PsiI
PspOMI PspXI PsrI PvuI PvuII RsrII SacI SacII
SalI SanDI SapI ScaI SexAI SfiI SfoI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI
SwaI TaqII TstI Tth111I XbaI XhoI XmaI XmnI
ZraI