(Linear) (Six-base) MAPSORT of: KIEE9603.seq  Check: 254  from: 1  to: 621



KIEE9603.orf

 With 182 enzymes: SgfI * 

                        March 22, 2012 18:32  ..

AhdI GACnn_n'nnGTC

Cuts at:      0     423     621 
   Size:        423     198
 
AlwNI CAG_nnn'CTG

Cuts at:      0      25     385     621 
   Size:         25     360     236

  Fragments arranged by size: 

                 360     236      25
 
BanII G_rGCy'C

Cuts at:      0     544     621 
   Size:        544      77
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     542     576     621 
   Size:        542      34      45

  Fragments arranged by size: 

                 542      45      34
 
BclI T'GATC_A

Cuts at:      0     140     621 
   Size:        140     481
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     538     621 
   Size:        538      83
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     181     247     621 
   Size:        181      66     374

  Fragments arranged by size: 

                 374     181      66
 
BsaWI w'CCGG_w

Cuts at:      0     316     621 
   Size:        316     305
 
Bsp1286I G_dGCh'C

Cuts at:      0     544     621 
   Size:        544      77
 
BspHI T'CATG_A

Cuts at:      0     585     621 
   Size:        585      36
 
BsrBI CCG'CTC

Cuts at:      0     497     621 
   Size:        497     124
 
BsrFI r'CCGG_y

Cuts at:      0      84     621 
   Size:         84     537
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0      25     621 
   Size:         25     596
 
BstBI TT'CG_AA

Cuts at:      0     562     621 
   Size:        562      59
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     402     621 
   Size:        402     219
 
EaeI y'GGCC_r

Cuts at:      0     209     621 
   Size:        209     412
 
EarI CTCTTCn'nnn_

Cuts at:      0     532     621 
   Size:        532      89
 
EcoO109I rG'GnC_Cy

Cuts at:      0     150     621 
   Size:        150     471
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     409     441     567     599     621 
   Size:        409      32     126      32      22

  Fragments arranged by size: 

                 409     126      32      32      22
 
HindIII A'AGCT_T

Cuts at:      0     328     621 
   Size:        328     293
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0      85     621 
   Size:         85     536
 
MspA1I CmG'CkG

Cuts at:      0     214     621 
   Size:        214     407
 
NaeI GCC'GGC

Cuts at:      0      86     621 
   Size:         86     535
 
NgoMIV G'CCGG_C

Cuts at:      0      84     621 
   Size:         84     537
 
PstI C_TGCA'G

Cuts at:      0      21     621 
   Size:         21     600
 
SapI GCTCTTCn'nnn_

Cuts at:      0     532     621 
   Size:        532      89
 
SfcI C'TryA_G

Cuts at:      0      17     621 
   Size:         17     604
 
SmlI C'TyrA_G

Cuts at:      0     196     262     621 
   Size:        196      66     359

  Fragments arranged by size: 

                 359     196      66
 
StyI C'CwwG_G

Cuts at:      0     518     621 
   Size:        518     103
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     275     307     621 
   Size:        275      32     314

  Fragments arranged by size: 

                 314     275      32
 

 Enzymes that do cut:

     AhdI    AlwNI    BanII     BcgI     BclI     BmrI    BpuEI    BsaWI
 Bsp1286I    BspHI    BsrBI    BsrFI   BstAPI    BstBI    BstXI     EaeI
     EarI EcoO109I    Hin4I  HindIII     MmeI   MspA1I     NaeI   NgoMIV
     PstI     SapI     SfcI     SmlI     StyI     TstI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII
   AflIII     AgeI     AleI     AlfI     AloI     ApaI    ApaLI     ApoI
     AscI     AseI    AsiSI     AvaI    AvrII     BaeI    BamHI     BanI
     BbeI     BbsI    BbvCI    BciVI    BfrBI     BglI    BglII     BlpI
 Bme1580I    BmgBI     BmtI     BplI     BpmI   Bpu10I     BsaI    BsaAI
    BsaBI    BsaHI    BsaXI    BseRI    BseYI     BsgI    BsiEI  BsiHKAI
    BsiWI     BsmI    BsmBI    BspEI    BspMI    BsrDI    BsrGI   BssHII
    BssSI   BstEII    BstYI  BstZ17I   Bsu36I     BtgI    BtgZI     BtsI
     ClaI    CspCI     DraI   DraIII     DrdI     EagI     EciI  EcoICRI
  Eco57MI    EcoNI    EcoRI    EcoRV     FalI     FseI     FspI    FspAI
    HaeII   HincII     HpaI     KasI     KpnI     MfeI     MluI     MscI
     MslI     NarI     NcoI     NdeI     NheI     NotI     NruI     NsiI
     NspI     PacI     PasI     PciI    PflMI     PfoI     PmeI     PmlI
     PpiI    PpuMI    PshAI     PsiI   PspOMI    PspXI     PsrI     PvuI
    PvuII    RsrII     SacI    SacII     SalI    SanDI     SbfI     ScaI
    SexAI     SfiI     SfoI     SgfI    SgrAI     SmaI    SnaBI     SpeI
     SphI     SrfI     SspI     StuI     SwaI    TaqII     TatI  Tth111I
     XbaI     XcmI     XhoI     XmaI     XmnI     ZraI